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Citations for Millipore Sigma :
7101 - 7150 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: Net ALI membrane resistance for each sample was determined by measuring the sample resistance with a MillicellERS-2 Voltohmmeter (Merck Millipore) then subtracting the blank sample resistance reading ...
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bioRxiv - Neuroscience 2024Quote: ... and these were immediately transferred into cell culture inserts over membranes of 0.4 µm pore size (PIC50ORG, Millipore, MA, USA), placed in 6-well culture dishes (Falcon ...
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bioRxiv - Microbiology 2023Quote: ... The elution buffer was further exchanged with Thermo Scientific BupH Tris Buffered Saline (TBS) by using Amicon Ultra-15 Centrifugal Filter Units with Ultracel-10 membrane cutoff (Merck Millipore) and protein concentration was measured with a Qubit 2.0 fluorometer.
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bioRxiv - Microbiology 2024Quote: ... Supernatants were concentrated using Centricon Plus-70 centrifugal filter units with a membrane pore size of 100,000 Da (EMD Millipore) according to the manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant proteins were resolved on a 10% SDS-PAGE and transferred to a nitrocellulose membrane (0.45 μm, Millipore, USA). Membranes were blocked in blocking buffer (5% nonfat milk ...
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bioRxiv - Cell Biology 2024Quote: ... 25-30µg protein was loaded on resolving 12% SDS-PAGE and later transferred to a methanol-activated PVDF membrane (Millipore-# IPVH00010) by using a semi-dry Trans blot apparatus (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: Proteins were separated by electrophoresis on precast gradient SDS-polyacrylamide gels and transferred to polyvinylidene difluoride (PVDF) membranes (Immobilon; Millipore) using a wet transfer tank (Hoefer Instruments) ...
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bioRxiv - Cancer Biology 2023Quote: ... the solution was filtered using a sterile syringe filter coupled to a filter unit with a low protein binding Durapore PVDF membrane (Merck Millipore) and the solution was poured into an amber tube to protect from light exposure.
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bioRxiv - Cell Biology 2023Quote: ... Protein was transferred to a PVDF membrane 0.2 μm (Amersham) and blocked in 5% Bovine Serum Albumin (BSA) (Sigma-Aldrich) for 20 min ...
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bioRxiv - Developmental Biology 2024Quote: ... The protein lysates were electrophoretically separated on sodium dodecyl sulfate-polyacrylamide gels and electrically transferred to polyvinylidene fluoride membranes (IPVH00010, Millipore). The membranes were blocked in 5% skimmed milk for 1 hour and incubated with the primary antibodies (Table S6 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred to a PVDF membrane 0.2 μm (cat#10600021, Amersham) and blocked in 5% Bovine Serum Albumin (BSA, cat#A9418, Sigma-Aldrich) for 20 min ...
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bioRxiv - Immunology 2023Quote: ... Immunoblot detection was achieved by exposure with a chemiluminescence imaging system (PXI Syngene, Ozyme) after membrane incubation with ECL (Immobilon Western, Millipore). The bands intensity values were normalized to that of β-actin using ImageJ software.
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bioRxiv - Cell Biology 2024Quote: ... were separated by 8 % sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS/PAGE) and then transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore) at 120 V for 2 hours using transfer buffer (25 mM Tris-base ...
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bioRxiv - Cell Biology 2024Quote: ... equal amounts of protein samples were loaded onto SDS–PAGE gels and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, USA) which were blocked with 5% bovine serum albumin (BSA ...
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bioRxiv - Genetics 2024Quote: ... Protein concentration of lysates was determined and 70 µg protein was loaded onto a 10% SDS-PAGE and subsequently transferred to a PVDF-FL membrane (Millipore). Membranes were incubated overnight at 4°C in primary antibodies (Flag ...
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bioRxiv - Immunology 2024Quote: ... the samples underwent sodium dodecyl sulfate-polyacrylamide gel electrophoresis for separation and were then transferred to a polyvinylidene fluoride membrane (Millipore). The membrane was blocked using the primary antibody-blocking solution and then incubated overnight at 4 °C with primary antibodies against P16 ...
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bioRxiv - Immunology 2024Quote: ELISpot assays for OVA-specific IgE and IgG1 antibody-secreting cells (ASCs) were performed using Multiscreen white 96-well filter plates with Immobilon-P PVDF membranes (Millipore). Plates were prepared by pre-wetting with 30 µL of 35% ethanol in water for 1 minute ...
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bioRxiv - Microbiology 2024Quote: ... Protein samples were separated in SDS-PAGE and transferred onto the Immobilon-P PVDF membrane (0.45-µm-pore-size, EMD Millipore). PSB15 protein was detected using affinity purified antibody (1:2000 ...
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bioRxiv - Immunology 2024Quote: ... in RPMI 1640 without serum for indicated times and the cell culture medium (supernatant) was collected and filtered using a membrane filter unit (SLGPR33RS, Millipore). Chloroform methanol purification of proteins from the filtered supernatant was performed and dried proteins were eluted with 1× SDS loading sample buffer and boiled at 96□°C for 10□min.
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bioRxiv - Bioengineering 2024Quote: ... or general membrane cell tracker (Cellvue Claret Far Red, PKH26 Red Fluorescent Cell Linker, or PKH67 Green Fluorescent Cell Linker, Sigma) following the manufacture’s protocol to facilitate downstream analysis ...
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... both the excised control spots and the peptide membranes were incubated with HRP-tagged anti-Biotin antibody (1:10,000, Sigma-Aldrich) and washed thrice again with Binding Buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were then incubated overnight with primary antibodies in 1X TBST containing 5% BSA (Sigma-Aldrich, St Louis, MO, USA). Membranes with multiple probes were stripped using 0.2 M NaOH before probing with the next primary antibody ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein samples were subjected to SDS-polyacrylamide gel electrophoresis and transferred to a PVDF membrane (Sigma-Aldrich). Membranes were blocked with 5% milk in TBST for 1 h and incubated with antibodies as listed in Table S1 ...
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bioRxiv - Cell Biology 2024Quote: ... mitochondria enriched heavy membrane fractions (200 μg) from control fibroblasts were chemically cross-linked with 1 mM dithiobis-sulfosuccinimidyl propionate (DSP) (Sigma) in HIM buffer ...
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bioRxiv - Cell Biology 2024Quote: ... The cell lysate was then separated by electrophoresis in a 10% polyacrylamide gel and transferred to a PVDF membrane (Merck Millipore). The membrane was blocked with 5% milk in PBS-T ...
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bioRxiv - Cell Biology 2024Quote: ... ShOpa1 transduced cells were lysed and 35 μg of protein was electroblotted to PVDF membranes (EMD Millipore, Burlington, MA, USA). The membranes were blocked and incubated overnight at 4°C with antibodies against anti-BNIP3 (3769) ...
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bioRxiv - Cell Biology 2024Quote: ... Extracted proteins were resolved on a 10% SDS-PAGE gel and electroblotted onto a PVDF membrane (Sigma Aldrich, Cat # IPVH00005). The membranes were blocked with 1X BlockerTM FL 10X Fluorescent blocking buffer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein samples were loaded onto SDS-PAGE gels and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, USA) which were blocked with 5% bovine serum albumin (BSA ...
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bioRxiv - Biophysics 2023Quote: ... 21 passes each through a 200nm and 100nm poly-carbonate Nuclepore Track-Etched Membrane (Sigma-Aldrich, St Louis, MO, USA).
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bioRxiv - Plant Biology 2023Quote: ... Membranes were blocked using 5% nonfat milk and probed with anti-poly-His primary antibody (cat. no. H1029; Sigma-Aldrich) at 1:3,000 dilution or anti- GST primary antibody (cat ...
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bioRxiv - Cell Biology 2023Quote: Approximately 100 µg protein of each sample was separated by 12% SDS-PAGE and transferred to an Immobilon-PVDF membrane (Merck Millipore). The blot was blocked with 5% non-fat milk in 1× TBST (20 mM Tris base ...
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bioRxiv - Cell Biology 2023Quote: ... P1 virus was used to infect 50 ml of SF9 culture and P2 virus was harvested after 3-5 days through centrifugation and subsequent filtration through 0.45 μm PVDF membrane (Merck Millipore, SLHV033RS). P2 virus was either propagated further or stored at 4ºC in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were resolved under denaturing and reducing conditions using 4 to 12% bis-tris gels (NuPAGE) and transferred to a polyvinylidene fluoride membrane (Immobilon-P, Millipore). Membranes were blocked with 5% nonfat dried milk and incubated with CAMKIIα (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... was prepared by filtering the spent media through a membrane filter with a 0.22 μm pore size (Merck Millipore, USA). MColorpHast pH indicator strip (Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... Direct blue staining was performed as follows: PVDF membranes were incubated for 10 minutes in 0.008% Direct Blue 71 (Sigma-Aldrich) in 40% ethanol and 10% acetic acid ...
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bioRxiv - Neuroscience 2022Quote: ... The retina was dissociated from the eyecup and mounted RGC side-up as a whole retina or cut in three or four pieces on black nitrocellulose membrane (0.8 mm pore size, Millipore, Ireland).
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bioRxiv - Neuroscience 2023Quote: ... membranes were subsequently blocked for 2 h at room temperature with 5% bovine serum albumin (BSA) (Sigma-Aldrich, MN, USA) in Tris buffered saline (TBS ...
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bioRxiv - Bioengineering 2023Quote: ... The resulting cell-modified graphite electrodes were encased in a dialysis membrane (14 kDa molecular weight pore size, Sigma-Aldrich) that was previously soaked in deionized water for 1 h before insertion in the electrochemical cell ...
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bioRxiv - Genetics 2023Quote: ... Proteins (5-15 μg) were separated on a 10% NuPAGE Bis-Tris Protein Gel and then transferred to a polyvinylidene difluoride membrane (Millipore). Membranes were blocked with 5% BSA made up in Tris-buffered saline-Tween 20 (TBS-T ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were re-probed with the HRP-conjugated primary anti-β-actin antibody for 20 min (Millipore-Sigma, 1:50,000), and the β-actin immunoreactivity was visualized.
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bioRxiv - Biochemistry 2022Quote: ... Then each membrane was incubated with the corresponding primary antibody (mouse IgG anti-Lewis antigen, Sigma–Aldrich, 1:500 dilution) for 1h at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... the membrane was washed twice with TBST and stained with Ponceau S stain [0.5% (w/v) Ponceau S (Sigma-Aldrich) in 1% (v/v ...
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bioRxiv - Biophysics 2023Quote: ... Samples were then transferred to a PVDF membrane and analyzed by Western blot using a mouse-anti-myc primary antibody (Sigma) and HRP-coupled goat-anti-mouse secondary antibody (Jackson ImmunoResearch) ...
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bioRxiv - Developmental Biology 2023Quote: ... The membrane was blocked with 5% milk or BSA (PAN-Biotech, P06-1391100) in Tris-Buffer Saline 0.2% Tween (Sigma, P9416) (TBS-T ...
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bioRxiv - Cancer Biology 2023Quote: ... All primary antibodies were incubated overnight with membranes in TBS blocking buffer supplemented with 0.1% Tween-20 (Sigma-Aldrich # P1379), while secondary antibodies (HRP conjugate ...
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bioRxiv - Neuroscience 2023Quote: ... #111.035.045) were used at 1:4000 for 1 h at room temperature and membranes were developed using Luminata Crescendo (Millipore). Images were acquired using Fusion Solo 7S Edge (Vilber).
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bioRxiv - Immunology 2023Quote: ... The eluted IgGs were buffered in 1 M Tris (pH=8.0) and then underwent buffer exchange to phosphate-buffered saline (PBS) and concentration using Amicon 30 kDa spin membranes (Millipore). The purified IgGs were stored at 4°C until further use.
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bioRxiv - Microbiology 2023Quote: ... The indexed PCR products were again normalized by loading 15 µl indexed PCR products onto Sequal Prep TM Normalization plates and were pooled and concentrated using a Amicon® Ultra 0.5 ml centrifugal filter (30K membrane, Merck Millipore). A NanoDrop™ One Microvolume Spectrophotometer (Thermo Scientific™ ...
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bioRxiv - Neuroscience 2023Quote: ... the primary neuronal cells X-irradiated with 50 Gy were put into co-culture with unirradiated BV2 microglial cells in NEUROBASAL MED SFM medium using a transwell co-culture membrane insert with pore size of 0.4 μm (Millipore, USA) until analysis.
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bioRxiv - Molecular Biology 2023Quote: ... Switzerland) and further processed by tangential flow filtration (30 kDa MWCO, PES membrane; Pellicon XL 50; Millipore, Merck, Darmstadt, Germany), autoclaved ...