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Citations for Millipore Sigma :
6951 - 7000 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... The proteins were separated by SDS-PAGE under reducing conditions and transferred to polyvinyl difluoride (PVDF) membranes (Millipore, Billerica, MA). Antibody incubations were performed in 3% BSA in TBS buffer using the following antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... gels and transferred (30 V; Thermo-Fisher mini-blot wet transfer system; 1 hr) to Immobilon FL polyvinylidene fluoride (PVDF) membranes (Millipore) for immunoblotting ...
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bioRxiv - Molecular Biology 2020Quote: ... The membrane was then blocked with 5% non-fat milk in phosphate buffer saline (PBS)-0.1% Tween20 (Sigma-Aldrich, P1379) for 1h at RT and probed with specific primary antibodies (α-p21 (1:500 ...
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bioRxiv - Molecular Biology 2020Quote: ... 30 μl of extract was resolved on 10 or 12% SDS-PAGE gels and after transfer the membranes were probed with 1:1000 dilution of anti-HA antibody (Sigma). Imaging of blots was done by Biorad ChemiDoc ...
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bioRxiv - Neuroscience 2021Quote: ... dissected hippocampi were cut into 400 μm slices with a tissue chopper and placed on a porous membrane (Millicell CM, Millipore). Cultures were maintained at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were incubated overnight at 4°C with the following primary antibodies: mouse anti-RELN G10 (MAB5364, Millipore 1:2000) or 142 (for human RELN ...
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bioRxiv - Neuroscience 2021Quote: ... Forty micrograms protein/lane was electrophoretically separated on a 10% SDS polyacrylamide gel and electrically transferred onto a Hybond-P PVDF transfer membrane (Millipore) for 1.5 h ...
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bioRxiv - Microbiology 2020Quote: ... 150 mM NaCl and 0.1% Tween 20) were performed before incubating the membrane with the anti-FLAG antibody 1:5000 for 1 h (Sigma). Next ...
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bioRxiv - Neuroscience 2021Quote: ... 200µl of total protein was further fractionated into soluble and aggregated forms using membrane based centrifugal filter unit (Millipore MRCF0R100) 500X g for 15 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... The fractions containing EfgA were pooled and concentrated in an Amicon centrifugal filter concentrator with a 10 kDa cutoff membrane (Millipore). The concentrated protein was then further purified by size-exclusion chromatography using a Superdex-200 column (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was washed with PBS-Tween 20 0.05% and incubated with peroxidase-conjugated anti-human IgG antibody (Sigma, USA) at a dilution of 1 ...
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bioRxiv - Microbiology 2020Quote: ... Samples containing equal amounts of protein were separated by SDS-PAGE (4% stacking and 12% resolving gel) and transferred onto a PVDF membrane (Millipore). The membrane was blocked with 5% skim milk (for detection of E ...
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bioRxiv - Neuroscience 2020Quote: ... Cold dissection media was used to separate and rinse the slices before placing them onto Millicell-CM membranes (Sigma-Aldrich). Slices were maintained in culture medium consisting of 25 % (vol/vol ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were resolved on 8-15% SDS-PAGE depending on protein size and transferred to polyvinylidene difluoride membrane (Immobilon-P, Millipore). Membranes were blocked with PBS-T (PBS ...
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bioRxiv - Immunology 2021Quote: ... equal amounts of cell lysates and immunoprecipitants were resolved by 10-12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and were then transferred to a polyvinylidene difluoride (PVDF) membrane (Millipore). After incubation with primary and secondary antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... Supernatant recovered after 48h from 293T transfected cells was filtered by a 0.45□μm pore membrane and added to MDA-MB-231 plated cells supplemented with 4□μg/mL polybrene (Sigma-Aldrich). After viral infection ...
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bioRxiv - Neuroscience 2020Quote: ... three organoids embedded in Matrigel (at day 21) and three unembedded organoids were manually dissected from transwell membranes and enzymatically digested with Accumax dissociation reagent (Sigma) with manual trituration ...
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bioRxiv - Cancer Biology 2021Quote: ... and 10-30 μg total cellular protein were resolved on SDS-PAGE and transferred onto Immobilon-FL PVDF membranes (Millipore). Bound antibodies were detected with IRDye infrared secondary antibodies using the Odyssey Infrared Imaging System (LI-COR Biosciences) ...
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bioRxiv - Neuroscience 2022Quote: ... was cut at the level of the somatosensory cortex and plated on Millicell-CM organotypic culture membrane inserts (Merck Millipore). Slices were cultured in a medium containing 50% Optimem-1 ...
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bioRxiv - Neuroscience 2022Quote: ... dissected hippocampi were cut into 400 μm slices with a tissue chopper and placed on a porous membrane (Millicell CM, Millipore). Cultures were incubated at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... The total protein extract was separated via SDS-PAGE and then transferred onto the 0.22 µm or 0.45 µm PVDF membrane (Millipore, Massachusetts, USA). The membrane was blocked with either 5% BSA (SRL ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were centrifuged at 15,000 x g for 15 min at 4 °C and the supernatants were separated in SDS–PAGE and transferred onto Immobilon-FL membranes (Millipore). Following blocking with 5% skim milk in PBS containing 0.05% Tween-20 (PBST ...
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bioRxiv - Genetics 2022Quote: ... Prepared cellular extracts were separated by 13 or 15 % SDS-PAGE and subsequently transferred to an Immobilon-PSQ Membrane (Millipore). The membrane was blocked by 1 % (w/v ...
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bioRxiv - Microbiology 2022Quote: ... Equal amounts (in micrograms) of lysates were separated on 4-15% SDS-PAGE gel and transferred to Polyvinylidene difluoride (PVDF) membrane (Millipore). Subsequently ...
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bioRxiv - Cancer Biology 2022Quote: ... Horseradish peroxidase conjugated secondary antibodies were added to the membranes (anti-rabbit, Invitrogen; 31460 and anti-mouse EMD Millipore; AP308P). Membranes were developed with ECL Western Blotting Substrate (Thermo Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... and cell surface biotin was cleaved off by incubating the cells on ice with 100 mM of membrane-impermeable reducing agent MESMA (2-mercaptoethane sulphonic acid (Sigma) as described (Nitzsche et al. ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was run on 12% acrylamide SDS-PAGE gel at a final concentration of 1 µM and transferred to polyvinylidene difluoride (PVDF) membrane (Millipore) using a semi-dry transfer apparatus ...
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bioRxiv - Cell Biology 2022Quote: ... After blocking in 5% BSA, membranes were incubated with Anti-FASN rabbit antibody (#3189, CST) antibody or mouse anti-tubulin antibody (Sigma). Protein was next incubated with HRP-conjugated secondary antibodies and was detected using chemiluminescence.
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bioRxiv - Bioengineering 2020Quote: ... the cells migrated through the membrane to the lower layer well were counted by H33342 staining (Sigma, St Louise, USA).
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... Protein samples were separated by sodium dodecyl sulfate (SDS)-PAGE and transferred onto a nitrocellulose membrane (Millipore, Temecula, CA, USA). After blocking with 5% non-fat powdered milk ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 μg proteins were separated by SDS-PAGE and electrotransferred onto Immobilon®-P transfer membranes (Merck Millipore, MA, USA), blocked with 5% skim milk in Tris-buffered saline with 5% Tween 20 (TBST) ...
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bioRxiv - Synthetic Biology 2020Quote: ... proteins were electrotransferred during a 30-min interval from the gel onto an Immobilon-P membrane of pore size of 0.45 µm (Merck Millipore, USA) under constant electric current of 0.1 A per gel and voltage of 5-7 V using Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... Equivalent amounts of protein or IP pulldown samples were resolved by SDS-PAGE and transferred to polyvinylidene fluoride (PVDF) microporous membrane (Millipore), blocked with 5% skim milk in TBS containing 0.1% Tween 20 (TBST) ...
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bioRxiv - Microbiology 2020Quote: ... and the resulting adhered bacteria on the cover slip were stained with 200 μL of the membrane stain N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide (FM4-64) (Sigma) at a final concentration of 20 μg/mL for 5 minutes ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto 45-μm polyvinylidene difluoride (PVDF) membrane (IPVH00010, Merck Millipore). Membranes were blocked using 5% non-fat dry milk in Tris-buffered saline with 0.1% Tween 20 (655204 ...
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bioRxiv - Microbiology 2020Quote: ... The unilamellar vesicles were prepared by extrusion of the hydrated lipid mixture through a 0.1 μm Whatman Nuclepore Track-Etched polycarbonate membrane (Sigma, USA) assembled in a LiposoFast Basic apparatus (Avestin ...
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bioRxiv - Immunology 2020Quote: ... 10-15 μg of proteins were loaded on to a 96-well plate with a polyvinylidene fluoride (PVDF) membrane at the bottom (Millipore-Sigma), which had been previously activated and primed ...
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bioRxiv - Neuroscience 2021Quote: ... were loaded on 10% acrylamide SDS-PAGE gels for separation by electrophoresis and then transferred onto PVDF membranes using a transblot (Millipore). The membranes were blocked with 5% milk in Tris-Buffered saline solution with Tween (TBST ...
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bioRxiv - Physiology 2020Quote: ... the membrane was incubated with rabbit anti-Urn8R (1:1000) and mouse anti-tubulin (1:2500; Sigma-Alrich, MO, USA) in blocking buffer supplemented with 0.2% Tween 20 (w/v) ...
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bioRxiv - Developmental Biology 2021Quote: ... The protein lysates were quantified and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (Millipore). This was followed by immunoblotting with primary antibodies NFATc1 (SAB2101576 ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins (40 μg) from cell homogenates were separated on a 10% SDS-polyacrylamide gel and electrotransfered to polyvinylidene fluoride membranes (Millipore). Each membrane was washed with Tris-buffered saline Tween-20 ...
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bioRxiv - Molecular Biology 2020Quote: ... The prepared samples were separated by SDS-polyacrylamide gel electrophoresis and transferred onto a polyvinylidene fluoride (PVDF) membrane (Merck Millipore). The PVDF membrane was blocked in 5% skimmed milk in Tris-buffered saline containing Tween for 1 hour and incubated with primary antibodies overnight at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... Mosquito homogenates were clarified by centrifugation at 20,000×g (4 °C for 30 min) and filtered through a 0.45 μm membrane filter (Millipore, Billerica, USA). Supernatants were collected and stored at −80 °C ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 120 mL of water sample was also collected per time point and filtered through a 0.2 µm PES membrane filter (Millipore, Inc.) for shotgun metagenomic analyses ...
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bioRxiv - Molecular Biology 2020Quote: ... equal masses of protein samples (20 µg) were resolved using SDS PAGE and then transferred to Immobilon-P PVDF membrane (Millipore). Recombinant NOCT (64-431 ...
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bioRxiv - Plant Biology 2021Quote: ... 2.5 to 40 µg of total protein extracts were separated on SDS-PAGE gels and blotted onto PVDF Immobilon-P membrane (Millipore). AGO1 protein was detected using the anti-AGO1 antibody (rabbit polyclonal ...
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bioRxiv - Immunology 2020Quote: ... MS2-FP8 VLPs were concentrated from SEC purified fractions by ultra-centrifugal filtration using Amicon® Ultra-15.0mL 100 K membrane (Merck Millipore Ltd. ...
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bioRxiv - Immunology 2021Quote: ... The CTCS was concentrated by centrifugal filtration (Centricon Plus-70, Ultracel-PL Membrane, 100 kDa size exclusion, Millipore Sigma # UFC710008), with ∼120 mLs CTCS concentrated to ∼0.5 mLs ...
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bioRxiv - Neuroscience 2020Quote: ... dissected hippocampi were cut into 350 μm slices with a tissue chopper and placed on a porous membrane (Millicell CM, Millipore). Cultures were maintained at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... The membrane was blocked (for 1 hr) and incubated for 1 hr with primary rabbit anti-FLAG antibody (Sigma-Aldrich). Following buffer wash the membrane was incubated for 1 hr with HRP-conjugated IgG mouse anti-rabbit antibodies (ThermoFisher Sci.) ...