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Citations for Millipore Sigma :
6801 - 6850 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... was used to perform HIV-1 entry assays The following reagents were used PEI (Sigma Aldrich, Servapore dialysis membranes (3.5 kDa MWCO ...
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bioRxiv - Neuroscience 2022Quote: ... Flies used in optogenetic assays were reared on food containing 0.25mM all-trans-retinal (Sigma-Aldrich) in darkness ...
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bioRxiv - Cell Biology 2022Quote: ... and the protein concentration of the supernatant assessed by Pierce 660nm protein assay reagent (Sigma, 22660). All samples were diluted to a final 1X Laemmli sample buffer (40% glycerol ...
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bioRxiv - Cell Biology 2022Quote: ... Reagents for the 3D sprouting angiogenesis assay were purchased from Sigma-Aldrich (Saint-Louis, MI, USA) (Fibrinogen/#F8630 ...
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bioRxiv - Genetics 2022Quote: Kidneys and cells were lysed in radioimmunoprecipitation assay (RIPA) buffer (Sigma-Aldrich, St. Louis, MO, USA) containing protease and phosphatase inhibitors (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... the impact of the various programs on cell viability was assessed by Resazurin viability assay (Sigma) in accordance with manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... a competition assay was performed in which 50 mM of 6-aminocaproic acid (ε-ACA) (Sigma) was added to the wells together with PLG ...
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bioRxiv - Plant Biology 2022Quote: ... Protein concentration was determined in the supernatants using Bradford protein assay method (Bradford reagent, Sigma–Aldrich), a standard curve was performed using BSA (bovin serum albumine ...
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bioRxiv - Neuroscience 2023Quote: Insulin was measured in EDTA plasma using a radio immuno assay (RIA, Merck Millipore, RI-13K). IAPP was measured by an enzyme-linked immunosorbent assay (ELISA ...
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bioRxiv - Biochemistry 2024Quote: ... and lysed with radio immunoprecipitation assay (RIPA) buffer containing 1% IGEPAL CA-630 (Sigma-Aldrich, I8896), 0.1% SDS (Carl Roth ...
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bioRxiv - Immunology 2024Quote: Luminex cytokine assay was performed utilizing the Mouse Cytokine/ Chemokine Magnetic Bead Panel (Millipore# MCYTOMAG-70K). Reagents were prepared as per kit instructions (including wash buffers ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell lysates for protein measurements were prepared by radioimmunoprecipitation assay (RIPA) buffer (20-188, Millipore-Sigma) supplemented with protease and phosphatase inhibitor cocktail (78440 ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell lysates for protein measurements were prepared by radioimmunoprecipitation assay (RIPA) buffer (20-188, Millipore-Sigma) supplemented with protease and phosphatase inhibitor cocktail (78440 ...
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bioRxiv - Biochemistry 2024Quote: ... All assays were carried out directly in a 96-well filtration plate (Millipore, Billerica, MA, USA) at room temperature and protected from light ...
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bioRxiv - Cell Biology 2024Quote: ... RPE cells were lysed in Radio- Immunoprecipitation Assay (RIPA) buffer containing protease inhibitor cocktail (Sigma- Aldrich). Subsequently ...
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bioRxiv - Systems Biology 2024Quote: ... The required amount of cells/assay were suspended in BME with 10% collagen I (Sigma Aldrich, St ...
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bioRxiv - Immunology 2024Quote: ... The multiparameter flow cytometry assays were carried out using Guava EasyCyteTM HT (Millipore Minneapolis, NM, USA), and the analyses were conducted using InCyte software (Millipore) ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by the addition of 60 µL NADPH (N7505, Sigma; 2 mg/3 mL assay buffer). The formation of TNB was monitored kinetically at 412 nm over 5 min in a spectrophotometer (Fluostar Omega ...
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bioRxiv - Neuroscience 2024Quote: ... After determining the protein concentration through a Bradford protein assay using Bradford reagent (Sigma, cat# B6916), each sample was diluted with an appropriate volume of RIPA buffer mixed with 1x Halt protease & phosphatase inhibitor cocktail to achieve a final protein amount of 20 µg protein ...
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bioRxiv - Neuroscience 2024Quote: ... individual organoids were collected after the assay’s completion and lysed in IX RIPA buffer (Sigma #R0278) for quantification with Precision Red (Cytoskeleton ...
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bioRxiv - Genomics 2024Quote: HCT116-POLR2A-AID2 cells were lysed using Radio Immuno Precipitation Assay (RIPA) buffer (Sigma-Aldrich, #R0278) with protease inhibitor added (Sigma-Aldrich ...
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bioRxiv - Biophysics 2024Quote: Cell metabolic activity was quantified thanks to a fluorescent resazurin-based assay (TOX8-1KT, Sigma-Aldrich). The assay was performed 2 hours (day 0 ...
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bioRxiv - Biochemistry 2024Quote: ... The enzymes of the coupled assay consisted of phosphoglucomutase (PGM from rabbit muscle, Sigma-Aldrich P3397) and glucose-6-phosphate dehydrogenase (G6PDH from L ...
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Transmembrane domains of H1 and H3 hemagglutinin contribute to membrane fusion in a different mannerbioRxiv - Biophysics 2024Quote: ... Bis[N,N−bis(carboxymethyl)aminomethyl]fluorescein (calcein) used in leakage assay was purchased from Sigma. 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(5-dimethylamino-1-naphthalenesulfonyl ...
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bioRxiv - Bioengineering 2024Quote: Cell viability was assessed using 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazoliumbromide reagent (MTT) assay (Sigma-Aldrich). After the treatment of interest or sham treatment ...
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bioRxiv - Cancer Biology 2024Quote: ... All cell lines were tested regularly to be mycoplasma-free by Mycoplasma detection assay (Sigma Aldrich).
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bioRxiv - Molecular Biology 2024Quote: ... A commercial cell proliferation kit (Millipore Sigma, Cell Proliferation Kit I (MTT)) was used for mesenchymal stem cells and endothelial cells ...
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bioRxiv - Bioengineering 2021Quote: ... Leukocyte (TRAP) Kit (Sigma). Immunohistochemistry analysis was performed on decalcified paraffin-embedded tissue sections using the HRP/DAB ABC IHC KIT (abcam ...
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bioRxiv - Cell Biology 2021Quote: ... Milliplex map kits (Millipore) were used to quantitate serum levels of human and mouse serum cytokines using a Luminex 200 (Luminex ...
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bioRxiv - Neuroscience 2023Quote: ... ECL-kit (Millipore-WBKLS0500), Bradford reagent (Bio-Rad-500-0006) ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were incubated with primary antibodies (Table 3) diluted in Intercept (TBS) blocking buffer with 0.05% Tween-20 (Sigma-Aldrich) overnight at 4°C on an orbital shaker.
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bioRxiv - Microbiology 2020Quote: ... Fifty µg of protein from each sample were resolved in a 12% (w/v) SDS–PAGE and transferred to polyvinylidene difluoride (PVDF) membranes (Merck Millipore). The phosphorylated fractions of the MAP kinase ...
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bioRxiv - Synthetic Biology 2021Quote: ... purified Sx-Cm-MOMP and Sx-CtE-MOMP proteins were spotted directly onto nitrocellulose membranes and incubated for 5 min before being blocked with 5% BSA (Sigma) and probing with the same primary antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were separated by SDS-PAGE gel electrophoresis, transferred to Polyvinylidene fluoride (PVDF, immobilon) membranes and blocked in 5% skimmed milk (Sigma) in TRIS-buffered saline (TBS ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were blocked with either Odyssey Blocking Buffer (Licor Cat #927-40000) or 5% skimmed milk in TBST buffer (SIGMA) and incubated for 1h at room temperature or overnight at 4° ...
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bioRxiv - Immunology 2021Quote: ... the membrane was incubated with anti-rabbit IgG antibody conjugated to horseradish peroxidase (Cat # A9044, Sigma Aldrich; 1:10,000 dilution) for 1 hr at RT and proteins visualized using Clarity ECL western blotting substrate (BioRad ...
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bioRxiv - Microbiology 2021Quote: ... Equal amounts of protein lysate were resolved by SDS-PAGE and subsequently transferred onto polyvinylidene difluoride Immobilon-FL membrane (Millipore). Following transfer ...
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bioRxiv - Molecular Biology 2021Quote: ... The proteins were separated by SDS-PAGE using a 10% polyacrylamide gel (Super SepTM Ace, Wako) and transferred to a PVDF membrane (Millipore). After transfer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Three more washing steps with TBS were performed before resolving the staining with 5 ml of substrate per membrane: a 30 mg tablet of 4-chloro-1-naphthol (Sigma) dissolved in 10 ml of methanol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 5 µg of protein lysates were loaded onto an SDS-PAGE gel and wet-transferred to 0.45 µM Immobilon-P PVDF membrane (Millipore, IPVH00010) at 200 mA for 90 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... 20 ug to 200 ug of protein was run on a 7.5 or 10% SDS-PAGE gel and transferred onto a PVDF membrane (Sigma-Millipore). The membranes were blotted for 1 hr in 1 ...
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bioRxiv - Developmental Biology 2020Quote: ... Fifty or thirty-five (depending on the protein detection) micrograms of proteins was separated on 9% Tricine gels and transferred to polyvinylidene difluoride membranes (Merck Millipore). The membranes were incubated separately with anti-Mmp-9 (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were further incubated with horseradish peroxidase-conjugated secondary antibody for 1 h and reacted with ECL (Millipore, USA). Finally ...
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bioRxiv - Cell Biology 2022Quote: ... and transferred into polyvinylidene difluoride (PVDF) membrane in 1× Transfer buffer (Tris-base/Glycine/Methanol, Sigma-Aldrich/Merck, Poznan, Poland) using the Mini Trans-Blot® system (Bio-Rad ...
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bioRxiv - Biophysics 2022Quote: ... The AFM experiments of NG108-15 cell membranes were performed in the Ca2+-free Ringer solution supplemented with 10mM TCEP (Cat# C4706, Sigma). The TCEP can breakdown the disulfide bonds ...
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bioRxiv - Plant Biology 2022Quote: ... The sample was then centrifuged twice at 13000 g for 10 min at 4°C and the supermnatent was passed through Millex® GV 0.22 μm PVDF membrane filter (Merck Millipore). Next ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein samples were separated in sodium dodecyl sulfate (SDS)-PAGE and transferred to polyvinylidene fluoride (PVDF) filter membranes (Millipore, USA) for immune-hybridization ...
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bioRxiv - Physiology 2022Quote: ... Membranes were subsequently incubated overnight at 4 °C with primary antibodies (1:2,000 monoclonal mouse anti-TRPA1 antibody, Sigma-Aldrich, and 1:3,000 MF20 mouse anti-myosin heavy chain antibody ...
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bioRxiv - Neuroscience 2021Quote: ... maintained for at least 1h at 4°C and then transferred onto semiporous membranes inserts (47 mm diameter, 0.45 µm pore size; Millipore FHLP04700) which were placed in six well tissue culture plates containing 1.1 ml medium per well ...
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bioRxiv - Neuroscience 2021Quote: ... The resolved proteins were then transferred to a nitrocellulose membrane at 220mA for 2 hours and the transfer efficiency was verified by Pounceau staining (Sigma). The membranes were then blocked for 1hour in 5% milk prepared in Tris Buffered Saline with 0.1% Tween-20 (TBS-T ...