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Citations for Millipore Sigma :
6901 - 6950 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Samples taken from the reactor for analysis of acetate and ammonium were immediately centrifuged and filtered with a 0.22 µm pore size filter (PVDF membrane, Millipore, Ireland). PHB and biomass dry weight measurements are performed on 15 mL bioreactor samples ...
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bioRxiv - Molecular Biology 2021Quote: The high molecular weight fraction of 0.33 mg dragline silk was dissolved in the ionic liquid and filtered through a 50k MWCO membrane (Amicon Ultra, Millipore), collected in 100 µL of lysis buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... The ionic liquid solution was dialyzed into lysis buffer (6 M urea, 2 M thiourea, 2% CHAPS, 1% DTT) using a 3k MWCO cellulose membrane (Amicon Ultra, Millipore).
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bioRxiv - Neuroscience 2021Quote: ... Equal amounts of 20 to 30 μg of protein were separated by SDS-PAGE and transferred onto PVDF membranes (Immobilion-P, Merck Millipore) according to standard protocols ...
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bioRxiv - Neuroscience 2021Quote: ... The DIG-labeled probes were hybridized to the membrane in Roche DIG Easy Hyb hybridization buffer (Millipore Sigma cat# 11603558001) at 49°C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... protein of each sample was separated with 12% sodium dodecyl sulfate-polyacrylamide gel and transferred to polyvinylidene difluoride membranes (Millipore). After blocked by 5% fat-free milk (Sangon Biotech ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was blocked in the blocking buffer of 5% BSA in TBST (137 mM NaCl, 2.7 mM KCl [Sigma-Aldrich P9541] ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were washed 3x in TBST (total wash time 1 hr) and were developed with luminata crescendo ECL substrate (Millipore) on an ImageQuant LAS 4000 (GE Healthcare) ...
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bioRxiv - Biophysics 2021Quote: 5−10 μg of proteins were separated on 10% SDS-PAGE and were consecutively electro blotted onto 0.45 µm nitrocellulose membrane (Millipore, USA). The membrane was blocked with 5% non-fat skim milk in PBS-0.1% Tween 20 for 2 hours at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... dechorinated with bleach and mounted between a semipermeable membrane (Lumox film, Starstedt, Germany) and a coverslip while embedded in Halocarbon 27 oil (Sigma). Excess oil was removed with absorbent paper from the sides to flatten the embryos slightly ...
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bioRxiv - Biophysics 2021Quote: All proteins were concentrated using ultrafiltration concentrators with either 3 kDa or 30 kDa MWCO regenerated cellulose membrane (EMD Millipore), flash frozen in small aliquots in liquid nitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were loaded onto self-made 4–13% or 4-10% polyacrylamide gradient gels separated and transferred onto PFDV membranes (Merck Millipore) followed by detection by specific antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... the nitrocellulose membranes were blocked with 5% non-fat dry milk in phosphate-buffered saline (PBS) 1X/0.1% Tween 20 (Sigma-Aldrich) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: Equal amount of protein lysates was loaded and resolved on SDS-PAGE gel and transferred onto an PVDF membrane (Millipore). Blots were blocked with 5% BSA for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: ... BT-12 coverslips and Transwell membranes containing both bEND3 and HIFko cells were cut and mounted on Mowiol 4-88 (Sigma) and imaged on a Zeiss LMS880 confocal microscope.
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bioRxiv - Cancer Biology 2022Quote: ... and 10 μg of proteins were separated by electrophoresis in an 10% SDS polyacrylamide gel and transferred onto a polyvinylidene fluoride membrane (PVDF, Millipore). The membranes were blocked with 5% (v/v ...
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HNRNPA2B1 controls an unfolded protein response-related prognostic gene signature in prostate cancerbioRxiv - Cancer Biology 2022Quote: ... Proteins were separated by size by SDS-Polyacrylamide Gel Electrophoresis (SDS–PAGE) on 10% w/v gels and electroblotted onto a Polyvinylidene fluoride or polyvinylidene difluoride (PVDF) membrane (3010040001, Sigma). Luminata Crescendo Western HRP substrate (10776189 ...
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bioRxiv - Neuroscience 2022Quote: ... The supernatant was collected and filtered through a 0.45μm membrane via centrifugation (Durapore PVDF centrifugal filters, Millipore, Billerica, MA, USA). The filtrate was kept on ice until analysis ...
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bioRxiv - Biochemistry 2022Quote: ... Membrane translocation in live cells was induced by the addition of 500 nM phorbol 12-myristate 13-acetate (PMA; Sigma) and monitored by imaging in 20–30 s intervals ...
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bioRxiv - Biochemistry 2022Quote: ... The refolded solution was left static over one day at 4 °C and concentrated with a compressed nitrogen-gas (or air) driven ultrafiltration device (350 ml Amicon Stirred Cell, 3 kDa NMWL membrane, Millipore), during which time cysteine in the solution gradually react to form cystine and precipitated out and could usually clog the ultrafiltration membrane at the end phase of ultrafiltration ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were then transferred to a prewet immobilon PVDF transfer membrane (Merck Millipore Ltd; #IPFL00010; Pore Size 0.45 μm) and sandwiched between wet filter paper and cassette holder ...
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bioRxiv - Cell Biology 2022Quote: ... the membranes were incubated with an alkaline phosphatase-conjugated secondary antibody directed against the first antibody (Sigma Aldrich, Taufkirchen, Germany) for 1 h at RT and developed in a solution of nitroblue tetrazolium chloride (NBT ...
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bioRxiv - Molecular Biology 2022Quote: ... the membrane was blocked for 15 minutes at room temperature in TBST containing 1 % BSA before adding HRP-Streptavidin (Sigma) to 1:20000 dilution and incubating for further 30 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were separated by electrophoresis in NuPAGE MOPS SDS running buffer and transferred in Bjerrum Schafer-Nielsen transfer buffer (48 mM Tris, 39 mM glycine, 20 % methanol) onto Immobilon-P PVDF membrane (Millipore) using Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... dialyzed in buffer C and then concentrated in a centrifugal concentrator with a 10-kDa molecular weight cutoff membrane (Merck Millipore).
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bioRxiv - Physiology 2022Quote: ... SDS-PAGE was performed as previously described.[28] Western blotting using fluorescence detection was performed using Immobilon-FL PVDF membrane (Millipore), Chameleon Duo pre-stained protein ladder ...
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bioRxiv - Neuroscience 2022Quote: ... Each 15 μg sample of protein was separated using 8% SDS polyacrylamide gel electrophoresis (SDS–PAGE) and transferred to a nitrocellulose membrane (Sigma). The membrane was blocked with 5% not-fat milk in 0.1M Tris-buffered saline (pH 7.4)/Tween 0,1% (TBSTween ...
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Core Microbiota Drive the Prevalence of Extracellular Antibiotic Resistome in the Water CompartmentsbioRxiv - Microbiology 2022Quote: All of the water samples were initially filtered by a vacuum filter with 0.2-μm pore-size polycarbonate membranes (Millipore, USA). The filters were used to extract iDNA by FastDNA™ Spin Kit for Soil (MP Biomedicals ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein was transferred onto a polyvinylidene difluoride (PVDF) membrane and blocked with Tris-Buffered Saline supplemented with 0.1% (vol/vol) of Tween 20 (Sigma®) (TBST ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were electrophoretically transferred to a 0.45 μm nitrocellulose membrane (Amersham) and their presence was analysed using HRP-conjugated anti-Flag (Sigma, A8592) and anti-α-tubulin (Abcam ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were resolved by SDS PAGE and transferred to an Immobilon P polyvinylidene fluoride or Immobilon-FL membrane (EMD Millipore)64 ...
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bioRxiv - Physiology 2022Quote: ... were coated with laminin (100 µg/mL from Engelbreth-Holm-Swarm murine sarcoma basement membrane diluted in PBS, Sigma-Aldrich) and stored in a 12-well plate (VWR) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells having invaded the bottom part of the membrane were counted after paraformaldehyde fixation and nuclei staining with DAPI (Sigma), as described (Renault-Mihara et al 2006).
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bioRxiv - Microbiology 2022Quote: ... Proteins were the resolved in a 12% SDS-PAGE gel and transferred on a 0.2 µm nitrocellulose membrane and then immunobloted using mouse FlagM2 antibody (Sigma-Aldrich) diluted at 1/500.
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bioRxiv - Molecular Biology 2022Quote: ... Ten micrograms of sample were separated on 10-12% SDS-PAGE gels prior to transfer onto Immobilon-P PVDF membranes (Millipore). Membranes were blocked in 5% milk and incubated overnight with primary antibodies diluted in 5% BSA ...
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bioRxiv - Biochemistry 2022Quote: ... SN1 was filtered using an Ultrafree-HV microcentrifuge filtration unit (0.45 μm pore size Durapore membrane, Millipore, Prod. No. UFC30HV00). This was accomplished by loading 500 μl aliquots of SN1 and centrifugation at 12,000g for 30 sec using one filtration unit per ml of SN1 ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then fractionated by 12% sodium dodecyl sulfate (SDS)/polyacrylamide gel electrophoresis (PAGE) on polyacrylamide gels and transferred to polyvinylidene difluoride membranes (Immobilon-FL, Millipore). Membranes were blocked with Odyssey Blocking Buffer at room temperature (RT ...
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bioRxiv - Cell Biology 2022Quote: ... β-actin protein level was evaluated by western blot analysis of rehybridized membranes with anti-β-actin antibody (Sigma, A5441) at a 1:5000 dilution ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amount of proteins (20 μg) were resolved by 10% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore). 5% skim milk powder or 5% bovine serum albumin in TBST (20 mM Tris-base ...
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MEK inhibitor resistance in lung cancer cells associated with addiction to sustained ERK suppressionbioRxiv - Cancer Biology 2022Quote: ... Membranes were incubated in primary antibodies (1:1000) overnight at 4° in 5% bovine serum albumin (BSA) (Sigma, A9647-100G), washed with TBST ...
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bioRxiv - Biochemistry 2022Quote: ... were separated on 0.1% SDS polyacrylamide gels (6–12% w/v depending on the molecular mass of protein) and electroblotted onto Immobilon-PVDF Transfer Membrane (Merck Millipore). Blots were blocked in 5% w/v non-fat milk or 5% BSA (Albumin ...
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bioRxiv - Biochemistry 2022Quote: ... membranes were equilibrated at 4 °C and incubated for 30 min in the presence of 2 mg/ml iodoacetamide (Sigma). Membranes were then solubilized in 10 mM HEPES ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were analyzed by separation on SDS-PAGE gels (8-12%) and transferred to polyvinylidene difluoride (PVDF) membrane (EMD Millipore). Immunoblots were imaged using an anti-Ssa1/2 polyclonal antibody at a 1:10,000 dilution ...
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bioRxiv - Cancer Biology 2022Quote: ... and 50μg of protein was resolved on 10–12% SDS-PAGE followed by transferring to nitrocellulose or PVDF membranes (EMD-Millipore). For immunoprecipitation ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins (30 µg) from brain cell lysate were resolved by SDS–PAGE and transferred to Immobilon-P membranes (Millipore Corp.). Membranes were incubated with anti-TSP1 (1:500 ...
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bioRxiv - Microbiology 2020Quote: ... Proteins from SDS-polyacrylamide gels were electrophoretically transferred to nitrocellulose membranes (Schleicher and Schuell) and subsequently stained with Ponceau S (Sigma) to check the loading of the lanes ...
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bioRxiv - Neuroscience 2020Quote: ... 50 −100 μg of protein were loaded on a 7%-12% polyacrylamide gel and then transferred on a methanol activated PVDF membrane (Millipore). Blot membranes were blocked for one hr with 5% milk or 5% BSA in TBS with 0.1% Tween 20 (TBST) ...
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bioRxiv - Cell Biology 2021Quote: ... Aliquots of cell lysate were resolved on 10% to 12% SDS-polyacrylamide gels and subsequently transferred to a polyvinylidene difluoride membrane (Millipore). The membrane was incubated with the following primary antibodies ...
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bioRxiv - Developmental Biology 2020Quote: ... separated in a 5-20% (w/v) gradient SDS/PAGE gel (SuperSep Ace; FUJIFILM) and transferred to a PVDF membrane (Millipore). Blots were blocked with Tris-buffered saline (TBS ...
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bioRxiv - Molecular Biology 2020Quote: ... and then with Laminin from Engelbreth-Holm-Swarm murine sarcome basement membrane (1.2 μg/mL in 1xPBS, 1 h at 37 °C, Sigma-Aldrich). They were passaged every two to three days in an appropriate ratio ...