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Citations for Millipore Sigma :
7251 - 7300 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... The protein was then concentrated to about 100 μM (Amicon Ultra-15, PLQK Ultracel-PL Membrane, 50 kDa, Merck, Millipore). After size-exclusion chromatography with a Superdex 200 Increase10/300 GL column (GE Healthcare) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Samples were loaded onto a 10% polyacrylamide gel and transferred to the Immobilon-FL PVDF Membrane (Millipore Sigma, no. IPFL00010). Membranes were blocked in Intercept LI-COR blocking buffer (PBS ...
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bioRxiv - Plant Biology 2024Quote: ... 15 µg of total protein extracts were separated on SDS-PAGE gels and blotted onto PVDF Immobilon-P membrane (Millipore). NBR1 was detected using the anti-NBR1 antibody (Rabbit polyclonal ...
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bioRxiv - Neuroscience 2024Quote: ... The basement membrane overlying BWMs was enzymatically removed by 0.5 mg/ml collagenase for 10 s (C5138, Sigma-Aldrich, Germany). Integrity of BWMs and nerve cord was visually examined via DIC microscopy.
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bioRxiv - Pathology 2024Quote: ... Membranes were blocked for 1 hour at room temperature with 10% albumin in PBS containing 0.05% Tween 20 (Sigma-Aldrich) and then incubated with the appropriate primary antibody overnight at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... the samples were mixed with Laemmli buffer and fractionated by SDS-polyacrylamide gel electrophoresis The separated proteins were transferred to a polyvinylidene difluoride membrane (Millipore), which was then incubated consecutively with primary antibodies ...
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bioRxiv - Molecular Biology 2024Quote: ... SDS-PAGE was performed using 4-20% Tris-Glycine gel (Beyotime, Shanghai, China) and then transferred to PVDF membranes (Millipore). Membranes were block for 2 hours with 5% non-fat dry milk in 1x PBST containing 25 mM Tris (pH 7.5) ...
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Novel RNA-binding protein YebC enhances translation of proline-rich amino acid stretches in bacteriabioRxiv - Molecular Biology 2024Quote: ... Proteins from the other gel were semi-dry transferred to a 0.45 µm NC membrane and western blotting was performed using 1:5000 dilution of Monoclonal ANTI-FLAG M2 antibody produced in mouse (Sigma).
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bioRxiv - Molecular Biology 2024Quote: ... protein bands were transferred from the gel by tank transfer onto a nitrocellulose or Immobilon-P PVDF membrane (Sigma-Aldrich) (3.5 hr ...
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bioRxiv - Molecular Biology 2024Quote: ... Equal amounts of protein (20 μg) were separated by SDS-PAGE on polyacrylamide gel and transferred to a PVDF membrane (Millipore). The membranes were blocked with 5% BSA in TBS-T (20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2024Quote: ... of 1 L each were filtered through 0.8 μm Whatman (Cytiva, Germany) Cellulose Nitrate membrane filters (25 mm diameter) using sterile 25 mm Swinnex (Merck Millipore, Germany) Filter Holders and a Vampire Sampler (Bürkle GmbH ...
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bioRxiv - Molecular Biology 2024Quote: ... of 1 L each were filtered through 0.8 μm Whatman (Cytiva, Germany) Cellulose Nitrate membrane filters (25 mm diameter) using sterile 25 mm Swinnex (Merck Millipore, Germany) Filter Holders and a Vampire Sampler (Bürkle GmbH ...
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bioRxiv - Microbiology 2024Quote: The supernatant from the 85% samples were concentrated and buffer exchanged with fresh MES buffer using 30 kDa molecular weight cutoff centrifugation filters with PES membranes (Millipore). The washed extracellular proteins were then resuspended in 0.5 mL MES buffer and transferred to 1.5 mL conical tubes ...
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bioRxiv - Microbiology 2024Quote: ... reactions were diluted 5-fold with dH2O if performing plasmid transformation or dialyzed using MF-Millipore 0.025µm MCE membranes (Millipore Sigma # VSWP02500) in dH2O for 1 hour if performing donor library transformation or rebooting phage ...
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bioRxiv - Immunology 2024Quote: ... bEnd.3 or ImmCPE cell lines were grown on transwell collagen-coated membrane 0.4um pore size inserts (CLS3495-24EA; Sigma-Aldrich) in a 24-well plate ...
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bioRxiv - Genomics 2024Quote: ... protein samples were separated by SDS‒PAGE using a 12.5% polyacrylamide gel and then transferred to a polyvinylidene difluoride (PVDF) membrane (Millipore, USA). The membrane was blocked with 5% BSA in Tween-PBS buffer and incubated overnight at 4°C with primary antibodies ...
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bioRxiv - Genomics 2024Quote: ... then loaded onto a 4-20% BioRad Criterion TGX Stain-Free precast gel and transferred onto an Amersham Protran nitrocellulose membrane (Sigma) for 1h ...
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bioRxiv - Genetics 2024Quote: ... Equivalent amount of protein samples were separated in 8% or 10% SDS-PAGE and transferred to polyvinylidene difluoride membrane (Millipore). After blocked with 4% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was immediately separated from the cells by filtration through a 0.45-µm pore size filter (PVD membrane, Merck Millipore Ltd.) ((adapted from [59]).
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bioRxiv - Bioengineering 2024Quote: ... proteins were transferred onto PVDF membranes and probed with antibodies against Vinculin (V9131, 1:1000, Sigma, St. Louis, MO, USA) and LDHA (#2012 ...
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bioRxiv - Bioengineering 2024Quote: ... The aqueous Bombyx mori silk fibroin (BmSF) solution was then subjected to dialysis using 12 kDa cellulose membrane (Sigma-Aldrich) against distilled water for 48 h with regular water changes at every 8 h ...
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bioRxiv - Biochemistry 2024Quote: ... The sample was then placed on a 200 mesh copper grid with an LR white resin membrane (Sigma Aldrich - L9774) and washed eight times for 2 minutes each with ultrapure water ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were then incubated for 1h at room temperature with the following primary antibodies (1/1000): Actin (MAB1501) from Millipore; Firefly Luciferase (ab21176) ...
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bioRxiv - Biophysics 2024Quote: ... The sample was centrifuged at 50,000g for 50 min at 4°C and the supernatant was filtered through a 0.22-μm polystyrene membrane (Millipore Sigma). The sample was incubated with Strep-Tactin XT resin (IBA Lifesciences ...
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bioRxiv - Cell Biology 2024Quote: ... 30-40 micrograms of proteins were separated on 12% SDS-PAGE gels and transferred to polyvinylidene fluoride (PVDF) Immobilon-P transfer membranes (Millipore). After washing in PBS and blocking in blocking buffer (5% BSA in PBST ...
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bioRxiv - Cancer Biology 2024Quote: ... Equal amounts of protein were separated in 10-15 % SDS-PAGE under reducing conditions and transferred onto a polyvinylidene fluoride (PVDF) membrane (Inmobilon-P, Millipore) using Mini Trans-Blot Cell System (Bio-Rad Laboratories) ...
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bioRxiv - Cell Biology 2024Quote: ... The membranes were blocked in 4% (wt/vol) non-fat milk in PBS + 0.1% (vol/vol) Tween-20 (Sigma-Aldrich). Primary and horseradish peroxidase-conjugated secondary antibodies were diluted in blocking buffer ...
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bioRxiv - Bioengineering 2024Quote: ... Proteins were subsequently separated via SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a polyvinylidene fluoride (PVDF) membrane (Merck Millipore). To prevent non-specific binding ...
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bioRxiv - Cancer Biology 2024Quote: ... proteins were separated through SDS-PAGE and then transferred onto polyvinylidene fluoride (PVDF) membranes from Sigma-Aldrich (Cat. No. IPVH00010). After blocking with a 3% BSA solution in Tris-buffered saline with 0.1% Tween-20 (TBST) ...
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bioRxiv - Cancer Biology 2024Quote: ... PVDF membranes were incubated for 1 h at room temperature with horseradish peroxidase-conjugated anti-rabbit IgG secondary antibody (Millipore). Blots were developed with enhanced chemiluminescence substrate (EuroClone ...
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bioRxiv - Cancer Biology 2024Quote: ... Equal amounts of protein were separated by SDS-PAGE and transferred to PVDF membrane (Millipore; Billerica, MA, USA; Cat# iPVH00010). Western blotting was performed using standard procedures ...
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bioRxiv - Cell Biology 2024Quote: ... 10-30μg total protein was loaded and separated by 10% SDS-PAGE gel and transferred to PVDF membranes (Millipore; GVHP29325). Then ...
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bioRxiv - Biochemistry 2024Quote: ... The PCR amplicon was digested with DpnI and desalted for 15 min by drop dialysis using a mixed cellulose ester membrane (0.025 µm size, Merck Millipore Ltd) and then used for transformation into the L ...
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bioRxiv - Cancer Biology 2024Quote: ... The protein was separated by sodium dodecyl sulfate-polyacrylamide (SDS) gel electrophoresis and transferred onto a polyvinylidene difluoride membrane (PVDF, Merck Millipore). The membrane was then blocked for 1 h with 5% skimmed milk and incubated with the following primary antibodies overnight followed with horseradish peroxidase (HRP ...
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bioRxiv - Developmental Biology 2024Quote: ... one membrane was probed for FLAG via mouse Anti-FLAG (1:1000 F1804 Sigma Aldrich, St. Louis, MO, United States) and the other membrane was probed for MYC via mouse Anti-MYC (1:1000 MA121316 Thermo Fisher) ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein samples were separated by 10% SDS-PAGE gel under 150V for 70 min and transferred on a PVDF membrane (Millipore) under 200 A for 90 min ...
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bioRxiv - Developmental Biology 2024Quote: ... Equivalent amounts of protein lysates non-incubated or incubated with WGA agarose beads were resolved on 8% SDS–PAGE gels and transferred to PDVF membranes (Millipore). Western blot analysis of equal protein loading was performed with the following primary antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein samples (10-20 μg) were separated by SDS-PAGE and transferred onto PVDF membranes (IPVH00010, Millipore). Membranes were blocked with 5% non-fat milk in TBS-T (Tris-buffered saline with 0.1% Tween-20 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... proteins were separated by 12% or 15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to PVDF membranes (Millipore, America). Membranes were blocked with 5% skimmed milk dissolved by TBST buffer at 37 ℃ for 2 h ...
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bioRxiv - Developmental Biology 2024Quote: ... Membranes where blocked using Intercept Blocking buffer and incubated with 1:2000 mouse anti-Flag M2 antibody (Sigma-Aldrich F1804), or 1:400 Rabbit anti-Birc5b (12) ...
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bioRxiv - Developmental Biology 2021Quote: ... the total amount of glucose was determined using 100 µl of glucose (HK) assay reagent (Sigma, G3293) following a 15 min incubation at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... For the OCR measurements the assay media was supplemented with 0.5 g.L−1 of glucose (Sigma, UK) and 2 mM of L-glutamine (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... The protein concentration was then determined using the Bradford protein concentration assay (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Molecular Biology 2020Quote: ... Cell viability assays were performed using WST-1 cell viability reagent according to manufacturer’s instructions (Sigma #18993700). In both assays ...
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bioRxiv - Biochemistry 2021Quote: ... Uptake of 3H-xanthine was measured with a rapid filtration assay using 0.45 μM nitrocellulose filters (Millipore). Reactions were incubated at 23 °C for the indicated periods of time and quenched by the addition of ice-cold 100 mM potassium phosphate ...
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bioRxiv - Bioengineering 2020Quote: ... was quantified with a Chloramin-T assay [43] with trans-4-hydroxyproline as a reference (Sigma, H5534). Before assaying ...
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bioRxiv - Cancer Biology 2021Quote: ... cell viability was evaluated using a colometric formazan-based cell viability assay (WST-1; Roche, Sigma-Aldrich).
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bioRxiv - Cell Biology 2021Quote: ... Rango nuclear import assays were carried out with or without 2.5 mM ATP (pH 7.5, Sigma-Aldrich) and HEK whole cell lysate (2.5 mg/mL in TRB) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed in radioimmunoprecipitation assay (RIPA) buffer(Boston, MA USA) supplemented with protease inhibitors (Sigma-Aldrich) and phosphatase inhibitor (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed directly on the 100 mm dishes using icecold radio-immunoprecipitation assay (RIPA) buffer (Sigma) supplemented with 0.1% Triton X-100 (Sigma ...