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Citations for Millipore Sigma :
6851 - 6900 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Equal protein concentrations were resolved via 8% SDS–PAGE and transferred to Immobilon-P polyvinylidene difluoride membrane (Sigma Aldrich; IPVH00010). Antibodies used ...
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bioRxiv - Neuroscience 2021Quote: ... The proteins were loaded and run on SDS-PAGE gels, transferred to PVDF membranes, and immunoblotted with antiKv2.1 antibody (K89/34, NeuroMab) and anti-α tubulin antibody (Sigma). The blots were detected using chemiluminescence reagent on a Bio-Rad Chemidoc MP and Image J was used for quantification of detected bands.
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bioRxiv - Molecular Biology 2020Quote: ... Protein samples were separated by Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) (814%) and transferred onto Polyvinylidene difluoride (PVDF) membranes (Millipore). Afterwards ...
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bioRxiv - Cell Biology 2022Quote: ... for 15 min and separated by 12% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) before being transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore). The membranes were blocked in 5% nonfat milk for 1 h and incubated overnight at 4 °C with the appropriate diluted primary antibodies or anti-flag/GAPDH antibody ...
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bioRxiv - Developmental Biology 2022Quote: ... The membranes were incubated with antibodies (total anti-HyYap serum was used at 1:1000 dilution, anti-actin (clone C4, Millipore) at 1:2000 dilution ...
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bioRxiv - Microbiology 2020Quote: ... Samples were heated for 5 min at 95°C prior to loading on 12% polyacrylamide gels followed by transfer onto NC membrane (Millipore). 3% BSA in phosphate buffer saline (PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein samples (5μg) were resolved by electrophoresis on 12% SDS–polyacrylamide gels and electrophoretically transferred to polyvinylidene difluoride (PVDF) membranes (Millipore Corporation) for 20 min at 25 V ...
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bioRxiv - Immunology 2021Quote: ... an amount of 15 μg of proteins was loaded on to a 96-well plate with a polyvinylidene fluoride (PVDF) membrane at the bottom (Millipore-Sigma), which had been previously activated and primed ...
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bioRxiv - Cell Biology 2020Quote: ... were fractionated by SDS-Page 10% and transferred to nitrocellulose membrane for immunoblot analysis using rabbit anti-GRP78 antibodies (GL-19, 1:5000 dilution from Sigma), anti-PERK antibodies (CS56833 ...
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bioRxiv - Developmental Biology 2020Quote: ... Proteins were then transferred onto polyvinylidene fluoride membrane (PVDF, Hybond-P, Amersham) in transfer buffer containing 12 mM Tris (Sigma), 96 mM glycine (BDH ...
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bioRxiv - Plant Biology 2020Quote: ... The sterilized eggs were collected from the membrane and placed on 25-μm-pore sieves in 0.01M 2-morpholinoethanesulfonic acid buffer (Sigma-Aldrich) under aseptic dark conditions for 3 days ...
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bioRxiv - Microbiology 2020Quote: ... Equal protein concentrations were loaded onto 12% SDS-PAGE gels and transferred to polyvinylidene fluoride membranes (Millipore Corporation, Billerica, Mass.). Membranes containing proteins were blocked in 5% BSA and incubated with rabbit anti-HMGB1 polyclonal antibody (1:1,000 ...
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bioRxiv - Cancer Biology 2020Quote: ... media of the 293T/T17 cultures was replaced and following 18 hours of incubation media containing viral particles were harvested and filtered through a 0.45 µm Durapore PVDF Membrane (Millipore SE1M003M00). Viral transfections were carried out over 72 hours ID8 parental cells and transduced cells were selected by their resistance to 2 μg/mL puromycin (MP Biomedicals 0219453910) ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein samples were heated and separated on sodium dodecyl sulfate (SDS)-PAGE or Tris-Acetate-PAGE gel-electrophoresis and transferred to PVDF membranes (Millipore). After blocking in phosphate-buffered saline (PBS)/Tween-20 containing 5% nonfat milk ...
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bioRxiv - Immunology 2021Quote: ... A total of 105 cells or 20 mg protein was resolved by SDS-PAGE and transferred to Immobilon-P PVDF membranes (Millipore). Membranes were blocked in 5% milk in TBS-T (25 mM Tris-HCl [pH 7.4] ...
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bioRxiv - Genetics 2021Quote: ... Equal amounts of protein were loaded and separated in 12% SDS-PAGE gels and transferred to Immobilon-FL PVDF membranes (Millipore). Membranes were blocked for 1 hour at room temperature with Intercept (TBS ...
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bioRxiv - Microbiology 2020Quote: ... The porous membranes were coated on both sides with collagen type IV from human placenta (0.5 mg/mL in water; Sigma-Aldrich) at room temperature overnight ...
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bioRxiv - Microbiology 2021Quote: ... was added to the culture (62) and cells were harvested by collecting the culture on a 0.22-micron membrane (Millipore GTTP). The filter membranes with cells were then cut into small pieces and stored at -80 °C until RNA extraction.
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bioRxiv - Neuroscience 2020Quote: ... ADAM10 and BACE1) or a Novex 10-20% Tris-Tricine gel (Aβ, C83 and C99) followed by blotting on nitrocellulose membrane (Millipore) using the following antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... the samples were separated by 5–20% gradient SDS-polyacrylamide gel electrophoresis (Nagaiki precast gels; Oriental Instruments, Ltd.) and transferred to polyvinylidene difluoride membranes (Millipore). After blocking with Odyssey Blocking Buffer-TBS (LICOR Biosciences ...
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bioRxiv - Neuroscience 2020Quote: ... Samples with equal amounts of total protein were separated in 11% SDS-polyacrylamide gel before electrophoretic transfer onto Immobilon-P membranes (#IPVH00010; Millipore). Membranes were cut horizontally ...
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bioRxiv - Neuroscience 2020Quote: ... The membrane was then stained and visualised for β-actin (rabbit anti-β-actin HRP, Sigma-Aldrich, A3854, 1:50,000) as described above.
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Membranes were probed with primary antibodies targeted against tyrosine hydroxylase (TH, 1:1000 dilution, EMD Millipore #AB152, Burlington, MA, USA) or β-Actin (loading control ...
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bioRxiv - Bioengineering 2020Quote: ... For western blotting 40 μg of protein samples were subjected to 10% in house SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto nitrocellulose membranes (Millipore). The membranes were blocked with 3% BSA in TBS containing 0.01 % Tween-20 for 1 h at room temperature and then incubated with specific primary antibodies at 4 °C overnight in the blocking buffer ...
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bioRxiv - Biochemistry 2020Quote: SDS samples were run on 10 % SDS-PAGE and transferred to 0.45 μm Immobilon-P PVDF Membrane (Millipore Sigma, IPVH00010) and immunoblotted using anti-FLAG (Sigma-Aldrich ...
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bioRxiv - Biophysics 2021Quote: Fractions were assessed by SDS-PAGE and pooled for dialysis in 3.5 kDa MWCO dialysis tubing (SpectraPor 3 Dialysis Membrane, Sigma Aldrich). For all variants except CLC ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were further incubated with secondary antibodies (mouse, 1:10000 dilution, A9044, Sigma; rabbit, 1:10000 dilution, A9169, Sigma). Immunoreactive bands were visualized by ChemiDoc XRS+ imaging system ...
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were blocked in 5% BSA TBS-T (5% BSA in 1x Tris Buffered Saline, 0.1% Tween 20, Sigma Aldrich) for 1 hour and incubated with primary antibodies overnight in 5% BSA TBS-T (MMP1 1:1000 ab137332 ...
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bioRxiv - Biophysics 2020Quote: ... The virus band was extracted and buffer exchanged to the NTE buffer using an Amicon Ultra-2 Centrifugal Filter Unit with Ultracel-100 membrane (Millipore).
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bioRxiv - Cancer Biology 2020Quote: ... Equal amounts of protein samples were subjected to SDS-PAGE and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, MA, USA). The membranes were treated with 1% blocking solution in TBS for 1 h ...
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bioRxiv - Cell Biology 2021Quote: ... the Phos-tag gels were incubated 4 × 10 min in 100 mM EDTA before electrophoretic transfer onto Nitrocellulose membrane (Sigma). Proteins were detected by immunoblotting using the following primary antibody concentrations in 5% milk (VWR)/Tris Buffered Saline -Tween20 (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of protein (11 µg) were run on a 10% SDS-PAGE gel and transferred to a polyvinylidene difluoride membrane (Millipore) overnight ...
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bioRxiv - Neuroscience 2020Quote: ... mitochondrial or synaptosomal fractions were prepared in Laemmli buffer and separated by SDS-PAGE before transfer to a PVDF membrane (Immobilon-P, Millipore). Membranes were blocked in 5 % non-fat milk in TBS-Tween 20 (0.1 % ...
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bioRxiv - Physiology 2020Quote: The membrane was washed in TBST and incubated with a peroxidase-conjugated secondary antibody (goat, anti-mouse IgG, Sigma-Aldrich) diluted 1 ...
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bioRxiv - Cell Biology 2021Quote: ... 30-50 micrograms of the protein were separated on a 15% polyacrylamide gel and electrophoretically transferred to PVDF membranes (Millipore). Membranes incubated with the primary antibodies against claudin-5 (1:1000 ...
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bioRxiv - Plant Biology 2020Quote: ... Total protein samples (30 μg) were resolved in 12%-SDS-PAGE and transferred to ImmobilonTM PVDF membranes (Millipore, Burlington, MA) in a semi-dry electroblotting apparatus (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... VLP samples were separated by 10% or 14% SDS-PAGE later blotted to PVDF membrane (Immobilon®-FL, Merck Millipore) and blocked overnight with ODYSSEY® blocking buffer ...
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bioRxiv - Microbiology 2020Quote: ... 20-30μg of protein extract was separated on a 4-20% precast polyacrylamide gel and transferred to a PVDF membrane (Millipore). Membranes were blocked with 5% nonfat milk in Tris-buffered saline (TBS ...
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bioRxiv - Microbiology 2021Quote: ... Aliquots of 2 ml of each recipient and donor strains were combined (three mating pairs in total) and filtered through sterile 0.22 µm membranes (Merck Millipore, GSWP02500), which were incubated over TSA plates for 8 days at 22°C ...
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bioRxiv - Neuroscience 2020Quote: ... whole cell lysates were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a 0.2μm pore-size nitrocellulose membrane (Millipore-Sigma, Burlington, MA). After transfer ...
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bioRxiv - Microbiology 2021Quote: ... Collected flow-throughs were pooled for replicates of the same sponge species and concentrated with Pellicon XL50 tangential filtering cassette with 100 kDa cutoff membrane (Millipore). The resultant concentrate (15-20 mL ...
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bioRxiv - Microbiology 2021Quote: ... Equal volumes of total lysates were analyzed using SDS-PAGE and transferred to a polyvinylidene difluoride (PVDF) membrane (Merck Millipore). Rabbit anti-BMV 1a antiserum (1:10,000 dilution ...
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bioRxiv - Immunology 2020Quote: ... Membrane was then stained with 1 μg/mL of HRP coupled anti-His (monoclonal mouse anti-polyHistidine-Peroxidase, Sigma-Aldrich) in 1% w/V BSA in PBS-T for 1 h at RT ...
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bioRxiv - Microbiology 2020Quote: ... Eight micrograms of protein per lane were separated by 12% SDS polyacrylamide gel electrophoresis and blotted onto Immobilon-P nylon membranes (Millipore). After transfer ...
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bioRxiv - Molecular Biology 2021Quote: ... crosslinked to the membrane by chemical treatment at 60°C using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) (Sigma) for 1 h 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was transferred to Hybond Nx nylon membranes (Amersham) and crosslinked using EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride) (Sigma) (Pall & Hamilton 2008) ...
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bioRxiv - Physiology 2020Quote: ... Protocol 2 (Pilocarpine): Six participants had 2 intradermal microdialysis membranes inserted and perfused with 1.67 mg/ml pilocarpine (Sigma-Aldrich) prior to the same B2R antagonist and agonist approach described in Protocol 1 (Figure 1B) ...
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bioRxiv - Microbiology 2021Quote: ... for 90 min at 120 V and blotted at constant 30 V for 30 min onto Immobilon-FL PVDF membrane (Merck Millipore). After the transfer ...
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bioRxiv - Plant Biology 2021Quote: ... Wild-type and mutated Pik-1 and Pik-2 proteins were detected probing the membrane with anti-FLAG M2 antibody (Sigma) and anti-HA high affinity antibody 3F10 (Roche) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Linear pST76-A plasmid was ligated with the PCR fragment using T4 ligase enzyme overnight at 16 °C followed by heat inactivation at 65 °C for 10 minutes and dialysis for 1 h on a 50 nm pore-sized membrane (Millipore). The ligation product was transformed into E ...