Labshake search
Citations for Millipore Sigma :
7051 - 7100 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... the titrations of total protein were separated on 12% SDS-PAGE gel followed by blotting to Immobilon PVDF membrane (Millipore). Membranes were dried and total protein was stained with Sypro Ruby (Thermo-Fisher ...
-
bioRxiv - Molecular Biology 2023Quote: ... were subjected to SDS-PAGE with 8% or 15% Tris-Glycine gels and transferred to Immobilon- P PVDF membranes (Millipore) using a TransBlot SD Semi-Dry Transfer Cell (BioRad) ...
-
bioRxiv - Microbiology 2023Quote: ... The resulting broth MSP was then filter-sterilized by using a 0.22-μm membrane filter unit (Millex®-GP, Merck Millipore Ltd ...
-
bioRxiv - Developmental Biology 2023Quote: ... The membrane was blocked with 5% milk or BSA (PAN-Biotech, P06-1391100) in Tris-Buffer Saline 0.2% Tween (Sigma, P9416) (TBS-T ...
-
bioRxiv - Physiology 2023Quote: Fractions were assessed by SDS-PAGE and pooled for dialysis in 3.5 kDa MWCO dialysis tubing (SpectraPor 3 Dialysis Membrane, catalog 132724, Sigma Aldrich). For all variants in pET28b except CLC ...
-
bioRxiv - Physiology 2023Quote: Purified GS proteins were assessed by SDS-PAGE and pooled for dialysis in 3.5 kDa MWCO dialysis tubing (SpectraPor 3 Dialysis Membrane, catalog 132724, Sigma Aldrich). Proteins were dialyzed at 25 °C for 4 hours against 25 mM Tris-HCl ...
-
bioRxiv - Plant Biology 2023Quote: ... the samples were loaded in SDS-PAGE and transferred to Immobilon®-PSQ Transfer Membrane (0.2 μM, Merck Millipore Ltd.) in 1x transfer buffer using a Bio-Rad transfer apparatus ...
-
bioRxiv - Biophysics 2023Quote: ... Samples were then transferred to a PVDF membrane and analyzed by Western blot using a mouse-anti-myc primary antibody (Sigma) and HRP-coupled goat-anti-mouse secondary antibody (Jackson ImmunoResearch) ...
-
bioRxiv - Developmental Biology 2023Quote: ... 2–3 endodermal explants (2PP or 3/4PP endoderm) were combined with 2–3 mesenchymal explants on Nucleopore membrane filters (Millipore) supported by fine meshed metal grids (Goodfellows) ...
-
bioRxiv - Neuroscience 2023Quote: ... We used direct blue stains of total protein as loading controls by saturating PVDF membrane for 10 min in 0.008% Direct Blue 71 (Sigma-Aldrich) dissolved in 40% ethanol and 10% acetic acid ...
-
bioRxiv - Microbiology 2023Quote: ... Samples were separated by SDS-PAGE on a 12% (vol/vol) acrylamide gel and transferred to polyvinylidene difluoride (PVDF) Immobilon-P membranes (Millipore). Primary antibodies specific for human IL-18 (MLB International PM014) ...
-
bioRxiv - Neuroscience 2023Quote: ... #111.035.045) were used at 1:4000 for 1 h at room temperature and membranes were developed using Luminata Crescendo (Millipore). Images were acquired using Fusion Solo 7S Edge (Vilber).
-
bioRxiv - Immunology 2023Quote: ... The eluted IgGs were buffered in 1 M Tris (pH=8.0) and then underwent buffer exchange to phosphate-buffered saline (PBS) and concentration using Amicon 30 kDa spin membranes (Millipore). The purified IgGs were stored at 4°C until further use.
-
bioRxiv - Cancer Biology 2023Quote: ... All primary antibodies were incubated overnight with membranes in TBS blocking buffer supplemented with 0.1% Tween-20 (Sigma-Aldrich # P1379), while secondary antibodies (HRP conjugate ...
-
bioRxiv - Microbiology 2023Quote: ... and splenocytes at 105 cells/well were added to 96-well high-protein-binding Immobilon-P membrane plates (Millipore, UK) that had been precoated with 5 µg/ml anti-IFN-γ monoclonal antibody (mAb ...
-
bioRxiv - Microbiology 2023Quote: ... The indexed PCR products were again normalized by loading 15 µl indexed PCR products onto Sequal Prep TM Normalization plates and were pooled and concentrated using a Amicon® Ultra 0.5 ml centrifugal filter (30K membrane, Merck Millipore). A NanoDrop™ One Microvolume Spectrophotometer (Thermo Scientific™ ...
-
bioRxiv - Immunology 2023Quote: ... Lysates were loaded on 10% home-made SDS- PAGE gel and transferred onto a 0.45 µm PVDF membrane (Millipore, IPFL00010) using TransBlot Turbo System (Bio-Rad) ...
-
bioRxiv - Plant Biology 2023Quote: ... Fractionated proteins were separated on NuPAGE 4-12% Bis-Tris mini gels (Life Technology) and transferred onto Immobilon-P membranes (Millipore) for immunoblotting analysis ...
-
bioRxiv - Molecular Biology 2023Quote: ... About 30 µg of total cell protein was separated on 8% SDS-PAGE and blotted onto Immobilon-P PVDF membrane (Millipore). The membrane was blocked in 0.2% I-Block (Tropix) ...
-
bioRxiv - Biophysics 2023Quote: ... The eluate was then concentrated using a 15 ml Amicon spin concentrator with a 100-kDa molecular weight cutoff membrane (Millipore) and purified by size exclusion chromatography (SEC ...
-
bioRxiv - Neuroscience 2022Quote: Samples were separated in standard SDS-PAGE gels and transferred to Immobilon-P PVDF membrane (Pore size 0.2 μM, EMD Millipore). The membrane was then blocked in 5% (w/v ...
-
bioRxiv - Neuroscience 2022Quote: ... Protein samples (40 μg) were separated by 4-10% SDS-PAGE and transferred to a PVDF membrane (Immobilon-P, Millipore). Membrane was stained with anti-OXPHOS (1:1000 ...
-
bioRxiv - Neuroscience 2022Quote: ... dissociated hippocampal cells were plated onto 1-μm pore and 30-mm diameter polyethylene tetra-phthalate (PET) membrane filter inserts (Millipore) that were matrix-coated with poly-L-lysine (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2022Quote: ... 20 μg of protein extract was resolved in 4-20% polyacrylamide SDS–PAGE gel and transferred onto polyvinylidene difluoride (PVDF) membranes (Immunobilon-FL, Millipore). After blocking with BSA in PBST (Phosphate-buffered saline with 0.1% Tween-20) ...
-
bioRxiv - Molecular Biology 2022Quote: 15-30 μg of protein extract were resolved on an 8% or 12% polyacrylamide-SDS gel and transferred to a polyvinylidene difluoride membrane (PVDF, Millipore). Primary antibodies were used at the recommended dilutions in 5% milk or 3% BSA diluted in TBS-Tween ...
-
bioRxiv - Microbiology 2022Quote: ... Membranes were cut and placed in a 24-well plate and permeabilised in 0.2% Triton-X100 (Sigma-Aldrich, United Kingdom) in PBS for 20 min at RT ...
-
bioRxiv - Microbiology 2022Quote: ... Pellet containing E64-treated schizonts were resuspended in cRPMI medium and filtered through a 2 µm Isopore polycarbonate membrane (Sigma) to purify free merozoites.
-
bioRxiv - Microbiology 2022Quote: ... The cell membrane and nuclei of the midgut tissue were labeled for 30 min with FM1-43 (5 μg/ml) (Sigma) and DAPI (0.5 μg/ml ...
-
bioRxiv - Microbiology 2022Quote: ... Samples were heated at 100°C for 5 minutes and then separated by SDS/PAGE and transferred to PVDF membranes (Millipore). Membranes were then probed with primary antibodies specific for murine caspase-11 (#C1354 ...
-
bioRxiv - Microbiology 2022Quote: ... The resulting virus-enriched filtrate was concentrated by tangential flow filtration (TFF) over a 30 kDa filter (Biomax 30 kDa membrane, catalogue #: P3B030D01, Millipore). Subsequently ...
-
bioRxiv - Neuroscience 2022Quote: Immunoblots were performed by resolving 10 μg of RAB or RIPA fraction through 10% denaturing SDS-PAGE and then transferred to polyvinylidene fluoride (PVDF) membranes (Millipore). Blots were probed by primary and secondary antibodies ...
-
bioRxiv - Plant Biology 2022Quote: ... 1mL of the supernatant was filtered through a syringe with an IC Millex-LG 13-mm mounted 0.2-mm low protein binding hydrophilic millipore (polytetrafluoroethylene [PTFE]) membrane filter (Millipore Ireland). The KTI in solution was separated on an Agilent 1260 Infinity series (Agilent Technologies ...
-
bioRxiv - Cancer Biology 2022Quote: ... The protein samples were separated by 10% SDS-PAGE gel and then transferred to a 0.22 μm polyvinylidene difluoride (PVDF) membrane (Millipore, USA) in a wet electron transfer device ...
-
bioRxiv - Molecular Biology 2024Quote: ... An equivalent of 1 μl of the original IVT was used for TCA precipitation and filtered onto a GF/C membrane (Millipore). The cap-analogue beads were washed 5 times with binding buffer and the final bead fraction suspended in SDS-PAGE sample buffer ...
-
bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein samples were subjected to SDS-polyacrylamide gel electrophoresis and transferred to a PVDF membrane (Sigma-Aldrich). Membranes were blocked with 5% milk in TBST for 1 h and incubated with antibodies as listed in Table S1 ...
-
The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... both the excised control spots and the peptide membranes were incubated with HRP-tagged anti-Biotin antibody (1:10,000, Sigma-Aldrich) and washed thrice again with Binding Buffer ...
-
bioRxiv - Bioengineering 2024Quote: ... or general membrane cell tracker (Cellvue Claret Far Red, PKH26 Red Fluorescent Cell Linker, or PKH67 Green Fluorescent Cell Linker, Sigma) following the manufacture’s protocol to facilitate downstream analysis ...
-
bioRxiv - Cell Biology 2024Quote: ... mitochondria enriched heavy membrane fractions (200 μg) from control fibroblasts were chemically cross-linked with 1 mM dithiobis-sulfosuccinimidyl propionate (DSP) (Sigma) in HIM buffer ...
-
bioRxiv - Immunology 2023Quote: ... Immunoblot detection was achieved by exposure with a chemiluminescence imaging system (PXI Syngene, Ozyme) after membrane incubation with ECL (Immobilon Western, Millipore). The bands intensity values were normalized to that of β-actin using ImageJ software.
-
bioRxiv - Cell Biology 2024Quote: ... ShOpa1 transduced cells were lysed and 35 μg of protein was electroblotted to PVDF membranes (EMD Millipore, Burlington, MA, USA). The membranes were blocked and incubated overnight at 4°C with antibodies against anti-BNIP3 (3769) ...
-
bioRxiv - Cell Biology 2024Quote: ... The cell lysate was then separated by electrophoresis in a 10% polyacrylamide gel and transferred to a PVDF membrane (Merck Millipore). The membrane was blocked with 5% milk in PBS-T ...
-
bioRxiv - Cell Biology 2024Quote: ... Extracted proteins were resolved on a 10% SDS-PAGE gel and electroblotted onto a PVDF membrane (Sigma Aldrich, Cat # IPVH00005). The membranes were blocked with 1X BlockerTM FL 10X Fluorescent blocking buffer (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein samples were loaded onto SDS-PAGE gels and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, USA) which were blocked with 5% bovine serum albumin (BSA ...
-
bioRxiv - Cancer Biology 2024Quote: ... Membranes were then incubated overnight with primary antibodies in 1X TBST containing 5% BSA (Sigma-Aldrich, St Louis, MO, USA). Membranes with multiple probes were stripped using 0.2 M NaOH before probing with the next primary antibody ...
-
bioRxiv - Molecular Biology 2024Quote: ... The gels were run at 150 V for approximately two hours at 4 °C and transferred onto 0.2 µm PVDF membranes (Millipore IPVH00010) using the Mini Trans-Blot cell system (BioRad 1703930 ...
-
bioRxiv - Microbiology 2024Quote: ... Proteins in the lysate were then fractionated by SDS-PAGE and transferred to a PVDF membrane (Merck Millipore, Cat#IPVH00010). After blocking with 1% nonfat milk overnight at 4°C ...
-
bioRxiv - Neuroscience 2024Quote: ... The supernatant was mixed with loading dye and separated on SDS -12% polyacrylamide gel and transferred onto PVDF Membrane (Millipore) before incubation with rabbit anti-OPTN (1:1000 ...
-
bioRxiv - Molecular Biology 2024Quote: ... protein was loaded onto a 10% or 8% SDS-PAGE gel and was transferred to Immobilon-FL PVDF membrane (IPFL00005, Millipore). The blot was blocked for one hour and hybridized with primary antibody overnight in 0.5% fish gelatin (G7041 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Membranes previously incubated with anti-RplA were incubated with the secondary antibody anti-rabbit IgG-HRP (#A8275, 1:10000, Sigma) for 1 h at room temperature and then washed three times with T-PBS ...
-
bioRxiv - Genomics 2024Quote: ... 1 mL of the supernatant was filtered through a syringe using an IC Millex-LG 13-mm mounted 0.2-mm low protein binding hydrophilic Millipore (polytetrafluoroethylene [PTFE]) membrane filter (Millipore Ireland).