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Citations for Millipore Sigma :
7151 - 7200 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... We used direct blue stains of total protein as loading controls by saturating PVDF membrane for 10 min in 0.008% Direct Blue 71 (Sigma-Aldrich) dissolved in 40% ethanol and 10% acetic acid ...
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bioRxiv - Physiology 2023Quote: Fractions were assessed by SDS-PAGE and pooled for dialysis in 3.5 kDa MWCO dialysis tubing (SpectraPor 3 Dialysis Membrane, catalog 132724, Sigma Aldrich). For all variants in pET28b except CLC ...
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bioRxiv - Physiology 2023Quote: Purified GS proteins were assessed by SDS-PAGE and pooled for dialysis in 3.5 kDa MWCO dialysis tubing (SpectraPor 3 Dialysis Membrane, catalog 132724, Sigma Aldrich). Proteins were dialyzed at 25 °C for 4 hours against 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... Afterwards, membranes were blocked with 5% BSA in TSMT (20 mM Tris; 150 mM NaCl, Merck; 1 mM CaCl2 (Sigma); 2 mM MgCl2 ...
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bioRxiv - Plant Biology 2023Quote: ... the samples were loaded in SDS-PAGE and transferred to Immobilon®-PSQ Transfer Membrane (0.2 μM, Merck Millipore Ltd.) in 1x transfer buffer using a Bio-Rad transfer apparatus ...
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bioRxiv - Developmental Biology 2023Quote: ... 2–3 endodermal explants (2PP or 3/4PP endoderm) were combined with 2–3 mesenchymal explants on Nucleopore membrane filters (Millipore) supported by fine meshed metal grids (Goodfellows) ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were electrophoretic separated on 4-20% SDS-PAGE precast gels (Beyotime) and transferred to a Hybond P polyvinylidene difluoride membrane (Millipore). The membrane was blocked in 5% skim milk for 2 h at room temperature then incubated with primary antibodies (Rabbit monoclonal to ATPB at 1/50000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... The beads were spun down and the supernatant was passed through a C8 membrane (3 M Empore™, Sigma-Aldrich), to remove any residual beads ...
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bioRxiv - Biophysics 2023Quote: ... Peak fractions were pooled using a 4 ml Amicon spin concentrator with a 100-kDa molecular weight cutoff membrane (Millipore) and used immediately for grid preparation or hydrolysis measurements.
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bioRxiv - Cell Biology 2023Quote: ... Cells were then incubated with a solution containing the membrane pore-forming protein Streptoylsin O (SLO) (Sigma catalog: S5265-25KU) sufficient to permeabilize 90% of HeLa cells ...
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bioRxiv - Microbiology 2023Quote: ... The resulting broth MSP was then filter-sterilized by using a 0.22-μm membrane filter unit (Millex®-GP, Merck Millipore Ltd ...
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bioRxiv - Molecular Biology 2023Quote: ... were subjected to SDS-PAGE with 8% or 15% Tris-Glycine gels and transferred to Immobilon- P PVDF membranes (Millipore) using a TransBlot SD Semi-Dry Transfer Cell (BioRad) ...
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bioRxiv - Molecular Biology 2023Quote: ... the titrations of total protein were separated on 12% SDS-PAGE gel followed by blotting to Immobilon PVDF membrane (Millipore). Membranes were dried and total protein was stained with Sypro Ruby (Thermo-Fisher ...
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bioRxiv - Plant Biology 2023Quote: ... Fractionated proteins were separated on NuPAGE 4-12% Bis-Tris mini gels (Life Technology) and transferred onto Immobilon-P membranes (Millipore) for immunoblotting analysis ...
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bioRxiv - Microbiology 2023Quote: ... Samples were separated by SDS-PAGE on a 12% (vol/vol) acrylamide gel and transferred to polyvinylidene difluoride (PVDF) Immobilon-P membranes (Millipore). Primary antibodies specific for human IL-18 (MLB International PM014) ...
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bioRxiv - Microbiology 2023Quote: ... and splenocytes at 105 cells/well were added to 96-well high-protein-binding Immobilon-P membrane plates (Millipore, UK) that had been precoated with 5 µg/ml anti-IFN-γ monoclonal antibody (mAb ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein extracts (40 μg) were separated via electrophoresis on 10% sodium dodecyl-sulfate-polyacrylamide gels and transferred to polyvinylidene difluoride membranes (Millipore). Membranes were blocked in 2% milk-phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a polyvinylidene difluoride (PVDF) membrane (IPVH00010, Millipore) using the Trans-Blot Turbo Transfer System (BioRad ...
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bioRxiv - Developmental Biology 2022Quote: ... MyoII:3xmKate2 or CellMask Orange Plasma membrane Stain (ThermoFisher; C10045; diluted 1:10 000 in ex vivo culture medium (Schneider’s medium (Sigma-Aldrich) supplemented with 10% Fetal bovine serum (Thermo Fisher ...
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Metabolomics reveals nucleoside analogs for regulating mucosal-associated invariant T cell responsesbioRxiv - Immunology 2023Quote: ... ELSIPOT assays were performed using a MultiScreen sterile 96-well filter plate with 0.45 um pore size of hydrophobic polyvinylidene difluoride (PVDF) membrane (Millipore S2EM004M99), antigen-presenting cells K562.hMR1 ...
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bioRxiv - Immunology 2023Quote: ... 50 µg protein in 1× Laemmli buffer was separated by SDS-PAGE and then transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore). The PVDF membranes were blotted with primary antibodies ...
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bioRxiv - Biochemistry 2023Quote: ... TEV-digested histones were then concentrated up to 4 mg/mL with ultrafiltration using Amicon YM50 membrane (MWCO 50 kDa) at 4 °C (Millipore). The concentrated sample was then injected onto a Superdex 200 10/300GL column ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM total lipid mixtures were extruded through a 0.05 μm pore size 19 mm polycarbonate membrane (Sigma, cat#WHA800308) with filter supports (Avanti ...
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bioRxiv - Microbiology 2023Quote: Protein samples were resolved by SDS–polyacrylamide gel electrophoresis and transferred to Immobilon®-FL polyvinylidene fluoride membrane (Millipore Sigma) as previously described [76] ...
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bioRxiv - Cell Biology 2023Quote: Samples were loaded on a NuPAGE 4-12% Bis-Tris protein gel and transferred onto an Immobilion PVDF membrane (Merk-Millipore) for 1 h at 35 V in a wet transfer apparatus buffered with 12.5 mM Trizma-Base (Sigma) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell extracts of equal total protein (20 μg each) were separated by SDS-PAGE and transferred to Nylon membrane (Merck Millipore). The membranes were blocked with 5% nonfat milk in TBST washing buffer (10 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates containing 50 µg protein were separated by electrophoresis on 8–12% polyacrylamide gels and the separated proteins were then electrotransferred to 0.45 μm PVDF membranes (#IPVH00010, Millipore, USA) at 200 mA for 2 h ...
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bioRxiv - Cell Biology 2022Quote: ... After transfer the membrane was blocked either by incubation in 5% dried milk (Marvel) in PBS with 0.1 % Tween-20 (Sigma-Aldrich) (PBST ...
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bioRxiv - Biochemistry 2023Quote: ... Ninety-six randomly selected clones from this transformation were grown in 500 µL AIM supplemented with 50 µg mL−1 kanamycin for 48 hours at 20 °C in 96-deep well plates sealed with Breathe-Easier membranes (Sigma). The culture medium was then separated from the cells by centrifugation for 15 minutes at 4500 x G and 10 µL of culture supernatant was assayed for xylobiose lysis using the protocol detailed in Controls for the detection of CAZyme activities in coupled reactions.
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bioRxiv - Microbiology 2023Quote: ... After blocking with 5% BSA the membrane was incubated for 1 h with 50 µg of purified PLG (Sigma-Aldrich) diluted in PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... 20-40 µg of total protein was separated on 7-10% SDS-PAGE polyacrylamide gels and transferred onto Immobilon PVDF membranes (Millipore). Membranes were blocked using 5% non-fat dry milk in TBS supplemented with 0.1% Tween-20 for 1 h at room temperature ...
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bioRxiv - Immunology 2023Quote: ... membrane at the bottom (Multiscreen HTS 0.45 μm Hydrophobic High Protein Binding Membrane 96-well Filtration plate (Product No. MSIPS4510; Millipore-Sigma) (65 ...
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bioRxiv - Neuroscience 2023Quote: ... Protein quantification was normalized to the total protein for each sample determined after transfer to the membrane using Ponceau S solution (Sigma).
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bioRxiv - Microbiology 2023Quote: ... The optimal amount of protein dissolved in 1× sodium dodecyl sulfate (SDS) loading buffer was separated on a 10% SDS-polyacrylamide gel and transferred to a polyvinylidene difluoride (PVDF) membrane (Millipore) for immunoblotting ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were washed and further incubated with secondary antibody (goat anti-mouse AP, 1:15000; Sigma Aldrich, St. Louis, MO). The membrane was washed with TBS-Tween 20 (TBS-T ...
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bioRxiv - Synthetic Biology 2023Quote: ... 200 μL of each sample was then transferred into a sterile 96-well plate and covered with a Breath-Easy membrane (Sigma). The plate was placed into a microplate reader and incubated at 37°C for 1 h (Tecan Spark ...
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bioRxiv - Immunology 2023Quote: ... Lysates were loaded on 10% home-made SDS- PAGE gel and transferred onto a 0.45 µm PVDF membrane (Millipore, IPFL00010) using TransBlot Turbo System (Bio-Rad) ...
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bioRxiv - Biophysics 2023Quote: ... The lysozyme protein solution was filtered by a 0.22 μm hydrophilic polyethersulfone (PES) membrane filter (Cat#SLGP033NS, Merck Millipore, USA). The filtered sample was stored in 1.0 mL aliquots at −45 °C until crystallization experiments were performed ...
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bioRxiv - Biophysics 2023Quote: ... The eluate was then concentrated using a 15 ml Amicon spin concentrator with a 100-kDa molecular weight cutoff membrane (Millipore) and purified by size exclusion chromatography (SEC ...
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bioRxiv - Neuroscience 2023Quote: ... Normalized total proteins were analyzed by electrophoresis in 10% polyacrylamide gels and transferred to PVDF membranes (Millipore, Billerica, MA, USA). Membranes were blocked in 5% skim milk (BD Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were washed 3x with TBS-T and subjected to chemiluminescence detection with Immobilon Western Chemiluminescent HRP substrate (Merck Millipore) using a Gel-Doc XR+ System (Bio-Rad) ...
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bioRxiv - Biophysics 2023Quote: ... The solution was filtered through a 0.22 μm and a 30 kDa centrifugal membrane filter (Millipore Sigma, UFC30GV and UFC5030). The amyloid fibrils were prepared by incubating 10 μM monomeric Aβ42 in phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2023Quote: ... the solution was transferred to a Millipore Amicon Ultra 30 kDa cut off membrane centrifugal filter unit (Merck Millipore, USA) (prewashed twice to remove glycerol ...
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bioRxiv - Molecular Biology 2023Quote: ... Supernatant was then centrifuged at 10,000 X g for 30 min at 4°C and filtered through a 0,2 μm membrane (Nalgene® bottle-top sterile filter, Z35223, Sigma). Small extracellular vesicles were pelleted at 100,000 X g for 1,5 h at 4°C by using Ti-45 rotor (Beckman Coulter ...
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bioRxiv - Neuroscience 2023Quote: ... dissociated hippocampal cells were plated on 1-μm pore and 30-mm diameter polyethylene tetra-phthalate (PET) membrane filter inserts (Millipore). These inserts were matrix-coated with poly-L-lysine and laminin on both the upper and lower surfaces ...
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bioRxiv - Molecular Biology 2023Quote: ... The eluted fractions were concentrated on Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-10 membrane (EMD Millipore, Merck, USA) and further purified by size exclusion chromatography using a Superdex 200 Increase 10/300 (GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2023Quote: ... The prepared samples were separated by SDS-polyacrylamide gel electrophoresis and transferred onto a polyvinylidene fluoride (PVDF) membrane (Merck Millipore). The PVDF membrane was blocked in 5% skimmed milk in Tris-buffered saline containing Tween for 1 hour and incubated with primary antibodies overnight at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... All samples were heat-denatured for 5 min at 100°C and run on a 4-20% Criterion Tris-HCl Protein gel and transferred to PVDF membrane (Millipore). Membranes with muscle tissue homogenates were blocked in 5% non-fat dry milk in TBS/0.2% Triton X-100 and incubated overnight in Tau12 (Millipore Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were blocked using 5% BSA or 5% non-fat dried milk in TBS containing 0.05% Tween 20 (Sigma-Aldrich) for 1h at 15-25°C ...
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bioRxiv - Neuroscience 2023Quote: ... Forty micrograms protein/lane was electrophoretically separated on a 10% SDS polyacrylamide gel and electrically transferred onto a Hybond-P PVDF transfer membrane (Millipore) for 1.5 h ...