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Citations for Millipore Sigma :
6801 - 6850 of 8078 citations for L Asparagine H2O 13C4 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... were seeded in a 200 μL total volume to white-bottom 96 well plates pre-coated with 0.01% poly-L-lysine solution (Sigma) and cultured overnight ...
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bioRxiv - Cell Biology 2024Quote: ... of 4.67 um diameter were washed (2000g for 1 min) and incubated with 0.05% poly-L-lysine (Sigma Aldrich) for 30 mins at RT on a rotating Eppendorf mixer ...
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bioRxiv - Microbiology 2024Quote: ... A 60 µl aliquot of this mating mixture was spotted onto a 0.45 µm pore mixed cellulose ester filter disc (Millipore) placed on solid LB containing DAP and incubated at 37 °C for 2 h.
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bioRxiv - Neuroscience 2024Quote: ... The interfaces were then coated with 0.1 mg/mL Poly-L-Ornithine solution (PLO) (Sigma-Aldrich, A-004-C) overnight in a fridge at 4 °C ...
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bioRxiv - Bioengineering 2024Quote: ... the solution was drop-cast on a coverslip previously coated with Poly-L-Lysine (Sigma Aldrich, Product Number: P4832) by incubating the coverslip with Poly-L-Lysine for 30 minutes at room temperature and washed with ddH2O afterward.
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bioRxiv - Plant Biology 2024Quote: ... Germany) and sodium bicarbonate (0.08 g/l) supplemented with Guillard’s F/2 Marine Water enrichment solution (Sigma-Aldrich, Belgium) and an antibiotic mixture (500 mg/l penicillin ...
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bioRxiv - Plant Biology 2024Quote: ... on Petri dishes containing KNOP medium (pH 5.8) (Waller et al., 2023) solidified with 7g/L Gelrite (Gelzan CM, G1910 Sigma). We maintained the plants by vegetative propagation (e.g. ...
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bioRxiv - Cancer Biology 2024Quote: ... Human astrocytes (Gift from the laboratory of Anders Nykjær, Aarhus University, Aarhus, Denmark) were cultured on poly-l-lysine (Sigma)-coated dishes and kept in astrocyte basal medium (ScienCell ...
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bioRxiv - Microbiology 2024Quote: ... Brain Heart Infusion Agar (BHI, Biokar Diagnostics) supplemented with 50 mg/L amphotericin (Sigma Aldrich, Saint-Louis, MO, USA) was used to count total aerobic bacteria after 48 h of incubation at 28°C ...
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bioRxiv - Neuroscience 2024Quote: The surface of each cover slip was coated with 0.5 mg/ml poly-L-lysine diluted in boric acid buXer (Sigma). Embryos were harvested from pregnant ...
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ITPK1 is an InsP6/ADP phosphotransferase that controls systemic phosphate homeostasis in ArabidopsisbioRxiv - Plant Biology 2020Quote: Seedlings were grown vertically on half-strength MS medium supplemented with 1 % sucrose and 7 g L−1 Phytagel (P8169, Sigma), pH 5.7 for 12 days (8 h light at 22°C ...
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bioRxiv - Genetics 2019Quote: ... the whole volume was placed into a 2 L flask with 30 g of Amberlite XAD-16 beads (Sigma-Aldrich) agitated at 200 rpm for 2 h at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... 10 g/L sodium chloride) medium supplemented with 50 μg/ml carbenicillin or 100 μg/ml kanamycin (both Sigma Aldrich), if applicable.
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bioRxiv - Biophysics 2020Quote: ... the culture was diluted as 1:100 with 1 L of same media with 0.2 mM ZnCl2 (Sigma Aldrich, 96468), and the culture was grown for 90 min at same condition such as culture with 10 ml volume ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Concentrated cell suspension (500 μl) was applied to glass-bottom dishes coated with poly-L-lysine solution (P8920, Sigma-Aldrich) and left for 10-30 min until cells were sufficiently adhered ...
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bioRxiv - Microbiology 2020Quote: ... dissolved in 0.57 g ddH2O was degassed for 45 min on ice with nitrogen followed by the addition of 100 µl KPS (0.036 g/l, Sigma, 379824). After another 15 min of degassing and the addition of 4 µl TEMED per ml monomer solution ...
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bioRxiv - Molecular Biology 2020Quote: ... 50 μl of Protein G magnetic beads were used and 10 μg of mouse monoclonal anti-FLAG primary antibody M2 (Sigma) were added before overnight incubation at 4°C with the lysate ...
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bioRxiv - Molecular Biology 2020Quote: ... Dissociated neurons were resuspended in normal medium and plated in 24-well plates containing 12-mm glass coverslips pretreated with 50 µg/ml poly-L-lysine (Sigma) in borate buffer ...
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bioRxiv - Cell Biology 2020Quote: ... The digested glomeruli were washed with 750 ⎧l 1X PBS + 0.04% BSA and filtered using a 40 ⎧m Flowmi Tip Strainer (Sigma BAH136800040). 1.25 ml 1X PBS + 0.04% BSA was added and the suspension was centrifuged at 500 xg for 8 minutes ...
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bioRxiv - Cell Biology 2020Quote: Every fish in this study was euthanized by supplying 1.5 g/L of ethyl 3-aminobenzoate methanesulfonate (MS-222, E10521, Sigma-Aldrich) to the fish tank water ...
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bioRxiv - Genetics 2021Quote: ... OSS cells were cultured in M3 + 10% FBS + 10% fly extract with 60 mg L-glutathione (Sigma-Aldrich, Cat#: G6013) and 10 µg/ml insulin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: Methylcobalamin (Vitamin B12 CAS Number 13422-55-4) and L-methionine (CAS Number 63-68-3) were purchased from Sigma and dissolved in water at the highest possible soluble concentrations to prepare stock solution ...
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bioRxiv - Developmental Biology 2021Quote: Mixed cerebellar cultures or glial fractions were prepared from P0-1 pups and cultured in serum-free “cerebellum” media (BME, Gibco; 2mM L-Glutamate, Gibco; 1% v/v BSA, Sigma; ITS liquid media supplement, Sigma; 0.9% v/v glucose ...
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bioRxiv - Developmental Biology 2021Quote: Cryosections (10 μm) were adhered to poly-L-lysine-coated slides and fixed in 4 % paraformaldehyde (Cat. # P6148, Sigma-Aldrich) solution in PBS for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: Mixed cerebellar cultures or glial fractions were prepared from P0-1 pups and cultured in serum-free “cerebellum” media (BME, Gibco; 2mM L-Glutamate, Gibco; 1% v/v BSA, Sigma; ITS liquid media supplement ...
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bioRxiv - Developmental Biology 2020Quote: ... Tissue dissociation was induced through addition of an equivalent volume of Papain solution (1 ml - 700 μl reagent grade water, 100 μl fresh 50 mM L-Cysteine (Sigma), 100 μl 10 mM EDTA ...
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bioRxiv - Microbiology 2020Quote: ... were cultured in erythroid differentiation medium (cPIMDM) composed of Iscove Basal Medium (IMDM) (Biochrom) supplemented with 4mM L-Glutamine (Sigma), 330 μg/ml holo-transferrin (BBI Solutions) ...
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bioRxiv - Cell Biology 2022Quote: Cells were synchronized in G1 using 1µM palbociclib and then released in S phase (see Cell cycle synchronization and DNA replication inhibition section) in the presence of nucleosides at the following concentrations: dC 7,3mg/L (D0776 from Sigma Aldrich); dG 8,5mg/L (D0901 from Sigma Aldrich) ...
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bioRxiv - Microbiology 2022Quote: CDw32 L cells (CRL-10680) were obtained from ATCC and were cultured in DMEM supplemented with 20% FBS (Sigma Aldrich) and Penicililin/Streptomycin/L-glutamine (PSG/Corning) ...
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bioRxiv - Neuroscience 2021Quote: Gli1 het and null mice were euthanized by CO2 narcosis and sciatic nerves were rapidly dissected and placed in cold L-15 media (Sigma). Nerve cultures from each biological replicate were maintained separately ...
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bioRxiv - Cell Biology 2020Quote: Leukocytes of healthy donors were counted and incubated in RPMI medium overnight over coverslips containing poly-L-lysine (Sigma-Aldrich), at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2021Quote: EdU staining was performed on in vitro cell culture slides or on glass coverslips in 24-well plates which were precoated with poly-l-lysine (Sigma) and laminin (Thermo Fisher) ...
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bioRxiv - Neuroscience 2021Quote: ... according to protocol of manufacturer and re-plated in plastic dish (Costar) coated with 100 μg/ml Poly-L-Lysine (Sigma) and 1 μg/ml Laminin (Sigma) ...
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bioRxiv - Molecular Biology 2020Quote: 10 μL samples containing 10 μM protein (TASOR variants or PARP1 catalytic domain) in the presence or absence of benzamide (Sigma) were loaded into glass capillaries (Nanotemper ...
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bioRxiv - Bioengineering 2022Quote: The glass bottom dishes (Matsunami, Osaka, Japan) were coated by 1 mg/mL poly-L-lysine (Mw: 30000–70000, Sigma) at room temperature for 1 h ...
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bioRxiv - Bioengineering 2022Quote: ... 1 g L−1 (NH4)2-H-citrate and 1 mL antifoam (Antifoam 204, Sigma-Aldrich, St. Louis, MO, USA). The medium was supplemented with with 2 mL L−1 trace elements solution and (1 M ...
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bioRxiv - Cell Biology 2022Quote: ... Frozen TauRD-Y and TauRD-Y* cells were thawed in arginine lysine deficient SILAC media (PAA) containing light (L) (Arg0, Lys0, Sigma) and heavy (H ...
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bioRxiv - Immunology 2020Quote: CDw32 L cells (CRL-10680) were obtained from ATCC and were cultured in DMEM supplemented with 20% FBS (Sigma Aldrich) and Penicililin/Streptomycin/L-glutamine (PSG/Corning) ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 μL of fresh sperm was mixed with a drop of HEPES-HTF medium containing 10% (w/v) polyvinylpyrrolidone (PVP, Sigma). Piezo pulses were used to separate the sperm head from the tail ...
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bioRxiv - Biochemistry 2019Quote: ... which was selected on uracil-deficient agar (1.39 g/L Yeast Synthetic Drop-out Media Supplements without uracil (Sigma-Aldrich), 6.7 g/L Yeast Nitrogen Base (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2019Quote: ... A single colony of BJ5464-NpgA-EcPKS1 was used to inoculate uracil-deficient broth (5 mL; 1.39 g/L Yeast Synthetic Drop-out Media Supplements without uracil (Sigma-Aldrich), 6.7 g/L Yeast Nitrogen Base (Sigma-Aldrich) ...
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bioRxiv - Synthetic Biology 2020Quote: Enriched M9 media was prepared using M9 salts (Difco M9 Minimal Salts) supplemented with 2 mmol L−1 magnesium sulfate (Sigma), 100 μmol L−1 calcium chloride (Sigma) ...
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bioRxiv - Synthetic Biology 2020Quote: ... the transformants were grown on synthetic complete uracil drop-out (Sc-URA) media with 1.92 g/l Yeast Synthetic Drop-out Medium Supplements without uracil (Sigma-Aldrich) and 6.7 g/l Yeast Nitrogen Base Without Amino Acids (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2019Quote: ... the mouse was removed from the cage and the 5 × 5cm Whatman paper containing 30µL of water was replaced with a 5 × 5cm paper of Whatman paper containing 30µL of propionic acid (Sigma Aldrich). The mouse was gently placed in section 3 and allowed to roam for 120 seconds in the cage ...
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bioRxiv - Cell Biology 2019Quote: ... 400 mM KCl, 2 mM MgCl2, 0.002% Brij35, 1 mM β-mercaptoethanol, 1 mM imidazole, 50 mM L-Arginine, Sigma#A5006 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... N-acetyl-DL-leucine was obtained from Molekula (#73891210) and N-acetyl-L-leucine was obtained from Sigma Aldrich (#441511).
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bioRxiv - Molecular Biology 2019Quote: In vitro BBB models were constructed and 1ml of serum-free EBM-2 medium containing 10 μg/mL HRP (0.5 mmol/L, Sigma-Aldrich) culture medium was added into the upper champer of the transwell system ...
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bioRxiv - Neuroscience 2019Quote: ... Explants were cultured in 35 mm tissue culture dishes pre-coated with poly-L-lysine (20 µg/ml for 1 hr; Sigma) and laminin (20 µg/ml for 1 hr ...
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bioRxiv - Microbiology 2019Quote: ... 500 μL of phage suspension (2 × 1010 PFU·mL-1) was mixed with 500 μL of extra pure chloroform (Merck Millipore™) and kept under 250 rpm·min-1 for 1 hour ...
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bioRxiv - Microbiology 2019Quote: ... Parasites were diluted to 0.3 parasites/100 μl and 100 μl of 2 % haematocrit culture was transferred to 96 flat-bottom plates in culture medium containing 200 mM L-glutamax (Sigma). After 7 days the media was changed and 0.2 % fresh blood added ...