Labshake search
Citations for Millipore Sigma :
6951 - 7000 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: PLA experiments were performed using the DuoLink In Situ Red Starter Kit Mouse/Rabbit (Millipore/Sigma-Aldrich # DUO92101-1KT) and following manufacturer protocol ...
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bioRxiv - Molecular Biology 2023Quote: DRIP-WB assay was performed using Magna Nuclear RIPTM (Native) RNA-Binding Protein Immunoprecipitation Kit (Millipore/Sigma # 17-10522) following the manufacturer’s recommended procedure with modifications ...
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bioRxiv - Molecular Biology 2023Quote: The quantification of glucose released in the preceding procedures was performed using the Glucose Assay Kit (Sigma-Aldrich, #MAK263) in accordance with the instructions provided by the manufacturer.
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bioRxiv - Cancer Biology 2023Quote: Nuclei from 10-15 million AMO1 cells were isolated using the Nuclei EZ Prep Nuclei Isolation kit (Sigma Aldrich), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Cytokine analysis was performed by Cincinnati Children’s Hospital Research Flow Cytometry Core per kit instructions (Milliplex MAP human protein panel, Millipore-Sigma).
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bioRxiv - Cell Biology 2023Quote: ... retinoic acid and epidermal growth factor (EGF) as per the SAGM Bullet Kit and 0.1 μg ml−1 cholera toxin (cat. no. C8052, Sigma), 5% FBS and 1% Pen/Strep ...
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bioRxiv - Molecular Biology 2023Quote: PLA experiments were performed using the DuoLink In Situ Red Starter Kit Mouse/Rabbit (Millipore/Sigma-Aldrich # DUO92101-1KT) and following manufacturer protocol ...
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bioRxiv - Bioengineering 2024Quote: ... the cells remaining in the microtube were labeled using the PKH26 lipophilic dye kit (Sigma-Aldrich, St. Louis, MO) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Cells harvested at required timepoints post-infection were estimated for ATP content using ATP Colorimetric Assay Kit (Sigma, MAK190) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: Nuclei were isolated from 10-μm fresh-frozen sections using the Nuclei PURE Prep Nuclei Isolation Kit (Sigma-Aldrich), with specific modifications ...
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bioRxiv - Bioengineering 2024Quote: The cell proliferation and viability after BNPs incubation was evaluated using cell counting kit-8 (cck-8, Sigma Aldrich), following the manufacturers’ instructions ...
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bioRxiv - Microbiology 2024Quote: ... These oligonucleotides were used to create a phage display library using the T7Select 415-1 Cloning Kit (Merck Millipore) using Liga5™ (A&A Biotechnology ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from cotton leaves using a Spectrum Plant Total RNA Kit (Sigma-Aldrich, Burlington, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... the Foxp3/Transcription Factor Staining Kit (eBioscience) was used accordingly with conjugated antibodies against Olig2 (clone 211F1.1; Sigma Millipore). An Attune Nxt (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... the Foxp3/Transcription Factor Staining Kit (eBioscience) was used accordingly with conjugated antibodies against Olig2 (clone 211F1.1; Sigma Millipore). An Attune Nxt (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... cells were washed once with PBS and RNA extraction was performed using GenElute Mammalian Total RNA Miniprep Kit (Sigma), according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA extraction from the thalli was carried out using the Spectrum Plant Total RNA kit from Sigma Aldrich. The quality and concentration of the extracted RNA samples were assessed using a Bioanalyzer (Agilent 2100) ...
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bioRxiv - Cell Biology 2024Quote: Glucose concentration in the medium was determined using the Glucose (GO) Assay Kit (Sigma-Aldrich, St. Louis, Missouri, USA) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... RNA was isolated from stressed and control plants (three biological replicates each) using SpectrumTM Plant Total RNA Kit (Sigma). Quality control of all the extracted RNA (triplicates for each condition ...
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bioRxiv - Physiology 2024Quote: ... The supernatant was then diluted 1:1 with sterile water and 20μL was used for glycogen measurement (Glycogen Assay Kit, Cat: MAK016, Sigma).
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bioRxiv - Microbiology 2020Quote: ... centrifuged at 21,100 x g for 5 min and cysteine content quantified using the Cysteine Assay Kit from Sigma-Aldrich according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA was extracted from 1 x 106 stable cells using the GenElute mammalian genomic DNA kit (G1N350, Sigma-Aldrich) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Serum concentrations of Metabolic Panel (Ghrelin, Glucagon, Insulin and Leptin) were determined using the Mouse Metabolic Magnetic Bead Panel Milliplex MAP kit (Millipore).
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bioRxiv - Microbiology 2021Quote: ... IgE) and IgA in feces supernatant were determined using the Mouse Immunoglobulin Isotyping Magnetic Bead Panel Milliplex MAP kit (Millipore) and Mouse IgE Single Plex Magnetic Bead Milliplex MAP kit (Millipore) ...
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bioRxiv - Microbiology 2020Quote: ... The initial rates of reaction were normalised to total protein content as determined using the QuantiPro BCA Assay Kit (Sigma). Control reactions without any substrate (G3P for GapA and GapN ...
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bioRxiv - Microbiology 2020Quote: ... This was then followed by genomic DNA removal and cleaning using the RNase-free DNase I Amplification Grade Kit (Sigma). All RNA samples were assessed for quality using an Agilent 2100 Bioanalyzer ...
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bioRxiv - Cancer Biology 2021Quote: Tail tip fibroblasts were seeded in 96-well plates and the mitochondrial membrane potential (MMP) was determined using the mitochondrial membrane potential kit (Sigma) following the recommendations made by the manufacturer ...
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bioRxiv - Cell Biology 2020Quote: ... BAC clone (RP11-293M10) (CHORI) was fluorescently labeled using Cy3-dUTP (ENZO) and nick translation kit according to the manufacturer’s instructions (Sigma-Aldrich). Human Cot-1 (DNA ...
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bioRxiv - Cell Biology 2020Quote: ... with thiobarbituric acid to form a colorimetric product by using a lipid peroxidation assay kit according to the manufacturer’s instructions (Sigma, USA). The optical density of the reaction product was examined at 532 nm ...
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bioRxiv - Immunology 2021Quote: ... A tertiary streptavidin-HRP antibody (a part of the Mouse ExtrAvidin® Peroxidase Staining Kit from Sigma at 1:1000) was added for 2 hr ...
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bioRxiv - Genomics 2020Quote: Between 250 ng and 2 µg of gDNA extracted from each tissue sample was bisulfite treated with an Imprint DNA Modification Kit (Sigma). Control samples lacking DNA template were run to ensure that there was no contamination during the bisulfite conversion of DNA ...
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bioRxiv - Genetics 2021Quote: ... were ground to a powder under liquid nitrogen and RNA was extracted using the Spectrum Plant Total RNA Kit (Sigma) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were concentrated using centrifugal filters and concentrations were measured using the Bicinchoninic acid (BCA) protein assay kit (Millipore Sigma). Proteins were aliquoted and stored at −80°C.
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bioRxiv - Genetics 2020Quote: Bisulphite conversions were carried out using the two-step modification procedure of the Imprint® DNA Modification Kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ChIP assay was used to detect the enrichment of HDAC3 in the promoter region of the miR-17-92 cluster and that of p65 in the Pgf promoter region using the EZ-Magna ChIP TMA kit (Millipore). The lung fibroblasts of each group in logarithmic growth phase were cross-linked and cultured with 1% formaldehyde for 10 min ...
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bioRxiv - Developmental Biology 2020Quote: ... The homogenate was heat inactivated at 70 °C for 5 min and then centrifuged at 14000 rpm for 3 min. Serum triglyceride determination kit (Cat. # TR0100) from Sigma was used to quantify triglyceride levels and protein levels were measured using the Quick Start™ Bradford 1X Dye Reagent (Cat ...
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bioRxiv - Molecular Biology 2022Quote: ... genomic DNA was extracted from 5 individual embryos at mid-gastrula (st. 11) or later employing the GenElute Mammalian Genomic DNA Miniprep Kit (Sigma). The genomic region containing the CRISPR / Cas9 targeted region was PCR amplified using a nested PCR approach (Table 1 ...
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bioRxiv - Cell Biology 2022Quote: ... The total concentration of proteins in samples was determined using the Bicinchoninic Acid Assay Kit (Sigma Aldrich/Merck, Poznan, Polska). The samples containing 20 µg of protein were mixed with 4× Laemmli loading buffer (Bio-Rad ...
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bioRxiv - Cell Biology 2019Quote: ... The activity of creatine kinase (CK) and lactate dehydrogenase (LDH) in plasma were measured using creatine kinase activity assay Kit (Sigma) and LDH activity assay kit (sigma ...
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bioRxiv - Neuroscience 2020Quote: ... The ROS assay was performed using a fluorometric intracellular ROS kit according to the supplier’s protocol (Sigma-Aldrich, Diegem, Belgium). In brief ...
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bioRxiv - Neuroscience 2020Quote: The mouse cortex between P0 and P2 was collected and dissociated with papain kit and DNase I (#LK003176, Worthington; 250U/ml; #D5025, Sigma). OPCs were purified by immnopanning approach according to our previous protocol (Lang et al. ...
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bioRxiv - Neuroscience 2021Quote: ... collected by centrifugation and superoxide anion production was determined by flow cytometry with Muse® Oxidative Stress Assay Kit (Millipore), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μL samples were used in a colorimetric assay according to the manufacturer’s protocol (NAD/NADH Quantitation Kit, Sigma-Aldrich) as described 21.
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bioRxiv - Molecular Biology 2020Quote: T7-Pep2,32 a complete human proteome library was restriction cloned into the T7-SEPARATE vector using the EcoRI/XhoI sites.32,33 The library was packaged in vitro using the T7Select Packaging Kit (Millipore Sigma) and expanded by plate lysate amplification to obtain an average clonal representation of ~100 plaques per peptide ...
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bioRxiv - Molecular Biology 2020Quote: ... the MILLIPLEX® MAP 6-plex Bcl-2 Family Apoptosis Panel 1 Magnetic Bead Kit (Millipore: Cat. No. 48-682MAG) was utilized ...
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bioRxiv - Molecular Biology 2020Quote: Lactate levels in the culture medium were measured using Lactate Assay Kit (Sigma-Aldrich, Cat#MAK064, Saint Louis, MO, USA) according to manufacturer’s instructions ...
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DNA writing at a single genomic site enables lineage tracing and analog recording in mammalian cellsbioRxiv - Synthetic Biology 2019Quote: ... All plasmids to be used for transfection were purified with HP GenElute Midi or Mini kits (Sigma # NA0200 and NA0150).
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bioRxiv - Molecular Biology 2019Quote: ... Black Gold staining was conducted according to the manufacturer’s instructions (Black Gold II myelin staining kit, EMD Millipore, Billerica, MA). Briefly ...
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bioRxiv - Plant Biology 2019Quote: ... Total RNA was extracted from 100 mg of whole seedling tissue using the Spectrum Plant Total RNA Kit (Sigma, STRN50), treated with DNaseI on columns (Qiagen ...
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bioRxiv - Developmental Biology 2019Quote: ... 3 ×106 cells were allocated per ChIP assay and prepared using the Magna ChIP A/G chromatin immunoprecipitation kit (Millipore). Cells were centrifuged (300g) ...