Labshake search
Citations for Millipore Sigma :
7051 - 7100 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: Cardiac tissues were fractionated into nucleus-enriched and cytoplasmic samples by using a CelLytic™ NuCLEAR Extraction kit (Sigma, Nxtract), as previously described (32).
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bioRxiv - Genetics 2022Quote: ... To assess editing efficiency genomic DNA was isolated from CRISPR-edited and control cells using a GenElute Mammalian DNA Miniprep Kit (Sigma) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Total RNA was extracted from 100 mg of whole seedling tissue using the Spectrum Plant Total RNA Kit (Sigma, STRN50) and l μl of eluted RNA was used for complementary DNA (cDNA ...
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bioRxiv - Microbiology 2020Quote: ... histolytica trophozoites were grown with and without queuine (0.1μM for three days) and total RNA was isolated using the TRI reagent kit according to the manufacturer’s instructions (Sigma-Aldrich). Three biological replicates from each treatment condition were processed ...
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bioRxiv - Genomics 2020Quote: ... nuclei were isolated by gently homogenizing tissue with a pestle in cold Nuclei Isolation Buffer from a Nuclei PURE Prep Kit (Sigma). Nuclei were pelleted and supernatant removed before being digested by incubating in 70 µl PBS ...
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bioRxiv - Genomics 2020Quote: CTCF Chromatin immunoprecipitation (ChIP) was performed on 1 × 107 Ter119+ erythroid cells using a ChIP Assay Kit (Millipore: 17-295) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: Protein concentrations of purified luciferase enzyme variants (+4G, +4C, +4A, and +4T) were quantified using the Bicinchoninic Acid (BCA) kit by following manufacturer’s protocol (Sigma-Aldrich). Equal amounts of each luciferase variant was diluted in Passive Lysis Buffer (Promega ...
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bioRxiv - Neuroscience 2021Quote: ... plasma was isolated from tail-blood and measured (3 μL in technical duplicates) using the Cholesterol Quantitation Kit (Sigma, MAK043) or the Triglyceride Quantification Colorimetric/Fluorometric Kit (Sigma ...
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bioRxiv - Physiology 2021Quote: The assay was performed using the In Vitro Tubulin Polymerization Assay Kit (≥99% Pure Bovine Tubulin; 17-10194 Sigma-Aldrich), according to the manufacturer’s instruction.
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bioRxiv - Cancer Biology 2021Quote: ... The PLA was performed using reagents supplied in the Duolink in situ Red PLA Mouse/Rabbit kit (Sigma-Aldrich, DUO92101) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Control line C7 was derived from a 41-year-old-female and reprogrammed using the Simplicon™ reprogramming kit (Millipore). Overexpression of the Yamanaka factors was introduced by a non-integrative ...
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bioRxiv - Neuroscience 2021Quote: ... 5-DIV cultured human spinal motor neurons and C5-C7 ventral horns of adult animals 3 days after surgery were estimated using a Rac1/Cdc42 Activation Assay Kit (#17-441, Merck Millipore). Briefly ...
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bioRxiv - Immunology 2020Quote: ... The elimination of the N-terminal His tag was performed through the cleavage with biotinylated thrombin included in the Thrombin Cleavage Capture Kit (Merck, Millipore) following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Total RNA was extracted from lung tissue or sorted cells as indicated in the text with Total RNA purification kit (Sigma) and treated with DNase I (Invitrogen) ...
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bioRxiv - Bioengineering 2021Quote: ... total DNA content was isolated from the 3D constructs at 14 and 21 days using a DNA extraction kit (Sigma). The isolated DNA contents were collected and quantified by using Nano drop (Thermo Scientific-2000).
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bioRxiv - Biochemistry 2021Quote: Total RNA was isolated using the Trizol method and mRNAs were isolated with the GenElute™ mRNA Miniprep Kit (Sigma). The concentration and purity of the mRNA were measured using a NanoDrop 2000 ...
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bioRxiv - Biophysics 2020Quote: ... Cell viability under 42 h compound treatment was determined via the Cell Counting Kit-8 (CCK-8, Sigma-Aldrich #96992) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... The Millipore Mouse Cytokine 32-plex kit was used to measure the serum concentration of 32 cytokines (Cat# MCYTMAG-70K-PX32, Millipore). xPONENT (Luminex ...
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bioRxiv - Cancer Biology 2022Quote: ... 1×105 cells were used to measure NADPH and NADP+ levels using a NADP+/NADPH colorimetric quantitation kit (Sigma-Aldrich).
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bioRxiv - Cell Biology 2022Quote: In situ proximity ligation (PLA) assay were performed using materials and guidelines provided with DuoLink PLA kit (Sigma-Aldrich, USA). Briefly ...
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bioRxiv - Cell Biology 2022Quote: Immunoprecipitation was performed using SRF or MKL1 antibody or control rabbit IgG with Magnetic Protein A/G beads (Magna ChIP A/G kit, Millipore), as described previously (Chatterjee et al. ...
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bioRxiv - Immunology 2019Quote: ... FCV (39, 40) and PSaV (32) infected cells was performed as described using the GenElute total RNA extraction kit (Sigma). In vitro transcribed RNAs were generated with T7 polymerase (New England Biosciences ...
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bioRxiv - Biochemistry 2019Quote: ... or doxycycline-treated (Dox) (1 µg/ml for 6 days) AW13516-shNPM1-luc+ cells using the GenElute Mammalian Total RNA Miniprep Kit (Sigma) followed by On-Column DNase I Digestion as per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... and the non-Rab Ras superfamily small GTPases (HRas and Arf1) were constructed using a pET-41 Ek/LIC vector kit (Novagen), as described previously [27 ...
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bioRxiv - Neuroscience 2019Quote: ... Sheared crosslinked chromatin was immunoprecipitated separately with an anti-AR antibody (3 μg, 17-10489 ChIPAb + androgen receptor Assay Kit, Millipore) or an equal amount of normal IgG (3 μg ...
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bioRxiv - Microbiology 2019Quote: ... Gal-3 levels were measured using commercially available enzyme-linked immunosorbent assay (ELISA) kits (Sigma-Aldrich, St. Louis, MO, USA) according to the manufacturer’s instructions.
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... 6 µl homogenate was mixed with 94 µl Glucose Oxidase (GO) reagent from the Glucose (GO) Assay Kit (Sigma, GAGO20) containing 1 µl (0.3 U ...
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bioRxiv - Microbiology 2019Quote: ... Genomic DNA was isolated from fresh bacterial cultures of the strains of interest using the GenElute Bacterial Genomic DNA Kit (SIGMA) according to manufacturers instructions ...
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bioRxiv - Microbiology 2019Quote: ... referred to here as hCMEC were obtained from Millipore and were maintained in an EndoGRO-MV complete medium kit supplemented with 1 ng/ml fibroblast growth factor-2 (FGF-2; Millipore) (81–84) ...
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Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: The amount of Shh secreted by mouse pancreatic tumor cells was quantified by using the Mouse Shh-N ELISA Kit (#RAB0431, Millipore), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... Digoxigenin (DIG)-labeled RNA probes were prepared by in vitro transcription with linearized constructs using DIG RNA Labeling Kit (SP6/T7) (Sigma). Embryos were fixed in 4% PFA in PBS ...
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bioRxiv - Physiology 2020Quote: ... Systemic levels of vascular markers were evaluated at the end of the Fc-GDF15 and vehicle treatment of obese ZSF1 rats by using multiplex MILLIplex kits (Millipore). The rat vascular injury panels included VEGF ...
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bioRxiv - Physiology 2020Quote: ... Kidney injury markers KIM-1 and NGAL were evaluated in serum from 20-week-old lean and obese ZSF1 rats by multiplex MILLIplex kits (Millipore). Serum NT-proBNP and rat cardiac injury markers (fatty-acid-binding protein 3 [FABP3] and myosin light chain 3 [Myl3] ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The MeRIP assay was carried out using the RNA-Binding Protein Immunoprecipitation Kit (Cat# 17-701, EZ-Magna, Millipore, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... The same protocol was used when the fluorescence reporter was substituted by a luminescence readout in this case we utilize 96 well white cell-culture plates and the Firefly Luciferase Assay kit from Sigma following the manufacturer protocol.
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bioRxiv - Molecular Biology 2019Quote: The protocol for detecting SINEUP RBPs was based on the protocol for the Magna ChIRP RNA Interactome kit (Merck Millipore) with the modification of cross-linking with 300 mJ/cm of 264 nm UV light (CL-1000 Ultraviolet Cross Link ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were allowed to recover for 36 hours after media change and Nuclear-Complex Co-IP was performed using commercially available Nuclear-Complex Co-IP kit from ActiveMotif(54001) with manufacturer’s recommended protocol using mouse Anti-Flag (Sigma F3165) or mouse Anti-His (Abcam 18184 ...
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bioRxiv - Pathology 2020Quote: ... and methyl cytosine levels assessed by a specific primary and secondary HRP-conjugated antibody system following the manufacturer’s instructions (Imprint® Methylated DNA Quantification Kit-Sigma). Following development with TMB ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Ki-67 immunostaining was performed according to manufacturer’s guidelines (BD Biosciences DAB substrate kit Cat.-550880, Ki-67 antibody-Millipore-AB9260). Ki-67 positive cells were counted manually (3 frames per section ...
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bioRxiv - Bioengineering 2020Quote: ... The hiPSC line LUMC0072iCTRL01 was obtained from the hiPSC core facility at the Leiden University Medical Center where they were generated from fibroblasts using the Simplicon RNA reprogramming kit (Millipore).
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bioRxiv - Molecular Biology 2020Quote: ... The cloning was performed according to the manufacturer’s protocol using the T7Select® 415-1 Cloning Kit (Millipore, 70015-3) with 1 μL of annealed oligonucleotides.
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bioRxiv - Developmental Biology 2021Quote: ... the cells were fixed with 4% paraformaldehyde and stained for TRAPase activity using a TRAPase staining kit according to the manufacturer’s protocols (Sigma-Aldrich, Acid phosphatase leukocyte ...
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bioRxiv - Microbiology 2020Quote: ... RNA was converted to cDNA using the First Strand cDNA synthesis kit for reverse transcription (avian myeloblastosis virus [AMV]; Millipore-Sigma). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Microbiology 2021Quote: Isotyping of the monoclonal antibodies and polyclonal sera responses was performed using the Mouse Monoclonal Antibody Isotyping Kit (Sigma-Aldrich), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: The anterior half of cortices from P0 mice were dissociated then cross-linked with 1% formaldehyde and processed using EZ-ChIP kit (Millipore). Chromatin fragments bound by endogenous mouse SOX5 were pulled down by anti-SOX5 (Abcam ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA was extracted from leaf tissue using the Spectrum Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, USA) following Protocol A.
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bioRxiv - Immunology 2020Quote: ... at a density of 50,000 cells/well for indicated time were fixed in 2.5% Glutaraldehyde and Tartrate-Resistant Acid Phosphatase in osteoclasts were stained using TRAP staining kit according to manufacturer’s instruction (Sigma-Aldrich).
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bioRxiv - Immunology 2019Quote: ... dead cells were stained by PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane Labeling (Sigma-Aldrich, MO, USA) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... Total RNA was isolated from control and stressed plants root samples using the Sigma plant RNA kit (Sigma, Inc, USA). RNA quality and concentration were determined by Qubit 2.0 fluorometer (Life Technologies ...
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bioRxiv - Molecular Biology 2019Quote: ... from each experiment was collected 72 h post-treatment and the amount of glucose was quantified using a colorimetric glucose assay kit (Sigma) following the manufacturer’s instructions ...