Labshake search
Citations for Millipore Sigma :
7201 - 7250 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Ratios between the oxidized form NAD+ and the reduced form NADH were measured using NAD+/NADH quantification kit (MAK037, Sigma) with whole cell lysates of wild type S ...
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bioRxiv - Genomics 2023Quote: ... The material was ground with mortar and pestle and the RNA was extracted with the Spectrum Plant Total RNA kit (Sigma) and treated with DNAse I amplification grade (Sigma) ...
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bioRxiv - Genomics 2023Quote: ... immunoprecipitated NS from the four S-phase fractions collected by flow cytometry or from an asynchronous cell population were amplified by whole-genome amplification (GenomePlex Complete Whole Genome Amplification kit #WGA2; Sigma) according to the manufacturer’s recommendations to obtain sufficient DNA amount ...
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bioRxiv - Genomics 2022Quote: ... Genome extraction was then performed using the GenElute Bacterial Genomic DNA (gDNA) extraction kit (Sigma-Aldrich, St. Louis, MO, USA) as per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... the supernatant was collected and used for ATP assay using an adenosine 5-triphosphate (ATP) bioluminescent assay kit (Sigma-Aldrich). The generated bioluminescence signal was measured with a SpectraMax M5 plate reader (Molecular Devices).
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bioRxiv - Cell Biology 2023Quote: The proximal ligation assay (PLA) was performed using the Duolink In Situ Orange Starter Kit Mouse/Rabbit (DUO92103; Sigma-Aldrich), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was extracted using GenEluteTM Mammalian Total RNA Miniprep Kit and On-Column DNase I Digestion Set (both Sigma-Aldrich) and a total of 9 samples fulfilling the submission requirements (0.5-1.0μg total RNA in nuclease free dH2O with A260/A280 and A60/A230 ratios above 1.8 ...
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bioRxiv - Genomics 2023Quote: ... The bisulfite-treated DNA fragments were ligated with a nine-nucleotide adapter and amplified using a GenomePlex Single Cell Whole Genome Amplification Kit (Sigma) for three rounds ...
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bioRxiv - Bioengineering 2023Quote: ... (Shrock, Shrock, and Elledge 2022) The ligated inserts were packaged with the T7Select packaging kit (Millipore Sigma, St. Louis, MO) as per manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... 1.5µl of haemolymph was used to measure the glucose and trehalose according to the Glucose assay kit (Sigma-Aldrich #GAGO20). To remove cells and tissue debris ...
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Critical contribution of mitochondria in the development of cardiomyopathy linked to desmin mutationbioRxiv - Pathology 2023Quote: ... The quantity of extracellular vesicles was evaluated by measuring the protein concentrations that were measured using Bicinchoninic Acid Kit (Sigma). Cardiomyocytes or spheroids were treated with pelleted vesicles corresponding to 0.1 mg of proteins per 25 000 cells every two days from day 10 until day 24 after the initiation of differentiation.
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bioRxiv - Pathology 2023Quote: Genomic DNA was isolated from mouse tail biopsies using the GenElute Mammalian Genomic DNA extraction kit (Millipore-Sigma, G1N350-1KT) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: Genomic DNA was isolated from mouse tail biopsies using the GenElute Mammalian Genomic DNA extraction kit (Millipore-Sigma, G1N350-1KT) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... and determination of the released glycerol in 10 µl of the upper phase with a commercial Glycerol Assay Kit according to the manufacturer’s instructions (Sigma-Aldrich).
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bioRxiv - Synthetic Biology 2024Quote: ... run on a 2.5 % (w/v) agarose gel at 100 V for 2 h and purified using a gel extraction kit (Sigma-Aldrich). The products were then used as templates for a second PCR with distinct combinations of barcoded primers (Supplementary Table 10 ...
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bioRxiv - Neuroscience 2024Quote: ... Acetyl Coenzyme A (CoA) levels in the brain tissues were analysed using Acetyl-Coenzyme Assay Kit (Sigma-Aldrich, #MAK039-1KT) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Glycerolipid content of male and female whole bodies and female ovaries and fat bodies was measured 12 days after dsGFP and dsXPO1 microinjection using the colorimetric Serum Triglyceride Determination kit (Sigma, Triglyceride Reagent T2449 ...
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bioRxiv - Microbiology 2023Quote: ... and DNA was extracted from a 500 mg crushed root sample using GenEluteTM Soil DNA Isolation kit (Sigma-Aldrich, Canada) with slight modifications ...
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bioRxiv - Microbiology 2023Quote: ... The insoluble matter was removed by centrifugation 10000×g for 5 min at 4 °C and protein was estimated using BCA kit (Sigma) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: The subtype of the mAb was IgG1 as determined using a subtype identification kit (Sigma-Aldrich, St. Louis, MO, USA). Non-immunoglobins in the ascites were removed by precipitation with caprylic acid ...
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bioRxiv - Neuroscience 2023Quote: ... Acyl-ghrelin concentrations in mouse plasma samples were determined by commercial ELISA kits (EMD Millipore, Billerica, MD; Catalog #EZRGRA-90K) (91).
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bioRxiv - Cancer Biology 2023Quote: The effect of Cul9 on GC cell proliferation was assessed by EdU assay using an In Vivo EdU Click kit (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The abundance of cytokines was determined using a MILLIPLEX Mouse 32-Plex Cytokine Panel 1 kit (Millipore # MCYTMAG-70K-PX32) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... Cytokine analysis was performed by Cincinnati Children’s Hospital Research Flow Cytometry Core per kit instructions (Milliplex MAP human protein panel, Millipore-Sigma).
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bioRxiv - Immunology 2023Quote: ... Neisserial genomic DNA was extracted from cell suspensions of overnight culture plates using the GenElute Bacterial Genomic DNA Kit (Sigma). Genomic libraries were generated using Illumina Nextera DNA Flex Library Prep according to the manufacturer’s instructions and sequenced on Illumina MiSeq 2×250bp paired end platform ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The concentration of tGSH (nM/mg of erythrocyte pellet) was assayed using a commercial kit (Sigma-Aldrich, St Louis, MO) and according to (Galván and Alonso-Alvarez ...
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bioRxiv - Plant Biology 2024Quote: Seedlings were harvested in liquid nitrogen and total RNA was extracted using a Spectrum Plant Total RNA kit (Sigma-Aldrich). The sequencing was made on an Illumina HiSeq 2500 system using 100-bp single-end reads protocol ...
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bioRxiv - Plant Biology 2024Quote: All samples were homogenised using Geno/Grinder 2010 (SPEX SamplePrep) and total RNA was isolated from homogenised samples using the Sigma Spectrum Plant Total RNA kit (Sigma) supplemented with the On-Column DNase I Digestion step to remove genomic DNA ...
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bioRxiv - Cancer Biology 2024Quote: ... The total ATP production was measure in nm/µg of protein by the ATP colorimetric assay kit (Sigma, MAK190-1KT) and experiment was performed as per provided manufacturer’s procedure ...
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bioRxiv - Neuroscience 2024Quote: ... Levels of individual cytokines were determined using a Milliplex bead assay kit (Milliplex MAP human cytokine/chemokine magnetic bead panel, #MCYTOMAG-70K; Millipore) and involving the Luminex MAPIX system with data analysis using xPONENT 4.2 software (Luminex) ...
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bioRxiv - Cell Biology 2024Quote: ... The amount of sialic acid in the liposomes was determined using a Sialic Acid Assay Kit (MAK314-1KT, Sigma Aldrich), and the concentration of liposomes containing a ganglioside was adjusted to the same level for use in microscopic observation.
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bioRxiv - Evolutionary Biology 2024Quote: ... kidney and gonads where available and from adult testis and ovaries with the GenElute Mammalian Total RNA Miniprep Kit (Sigma) and DNase treated using DNA-free (Ambion ...
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bioRxiv - Biochemistry 2024Quote: ... 400 μg total RNA in 250 μl water was applied to a GeneElute mRNA miniprep kit (Sigma-Aldrich; MRN70-1KT), following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Aβ and pTau levels in the organoids were quantified with Milliplex human amyloid beta and tau kit (Millipore HNABTMAG-68K) on the Luminex ...
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bioRxiv - Cancer Biology 2024Quote: ... The isolation of nuclear proteins was achieved with the CelLytic™ NuCLEAR™ extraction kit (Sigma Aldrich, St. Louis, MO) using the manufacturer’s recommended protocol ...
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bioRxiv - Neuroscience 2024Quote: Binding of cGAS and cytoplasmic mtDNA in human and mouse brain sections was determined by a PLA kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... in primary neuronal lysate after Aβ or gAβ treatment from mouse or human brain were quantified by the Glycerol-3-Phosphate Assay Kit (Sigma), High Sensitivity DHAP Fluorometric Assay Kit (Sigma) ...
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bioRxiv - Physiology 2024Quote: ... tissues were homogenized in 150 μL of ATP Assay Buffer from the ATP Colorimetric/Fluorimetric Assay Kit (Sigma-Aldrich, MAK190). For protein extraction ...
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bioRxiv - Microbiology 2024Quote: ... The plasmid containing the desired complement was extracted from the confirmed colonies using GenElute™ Plasmid Miniprep Kit (Sigma-Aldrich). The plasmid containing the required complement was then electroporated into the phage-resistant mutants ...
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bioRxiv - Microbiology 2024Quote: The effect of GpsB on the inorganic phosphate released by FtsZ and truncated FtsZ was examined using malachite green phosphate assay kit (Sigma). Briefly ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA from control and treated samples was extracted after Sirodesmin PL treatment using a Plant Spectrum Total RNA Kit (Sigma), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: Approximately 100 milligrams of tissue from either in vitro-grown plantlets or leaf tissue from greenhouse-grown plants was used for genomic DNA extraction (GenElute™ Plant Genomic DNA Miniprep Kit, Sigma). PCR was performed using primers SWEET10a_g-F and SWEET10aPro-R (Table S1) ...
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bioRxiv - Plant Biology 2020Quote: ... which was extracted from the pool of six biological replicates of both control and salt treated plants by using Sigma spectrumTM Total RNA kit (Sigma, USA) and cDNA synthesis was performed by using the PrimeScriptTM 1st strand cDNA synthesis kit (TAKARA ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell pellets were snap frozen in liquid nitrogen and nuclei were extracted from cell pellets (Sigma Nuclei Isolation Kit #NUC-101). Chromatin was fragmented in sonication buffer (50mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: Periodic acid-Schiff (PAS) staining was performed to stain mucin-producing goblet cells in murine EDMs using a PAS Kit (Sigma-Aldrich) as described previously [8] ...
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bioRxiv - Immunology 2021Quote: ... ROS release was monitored for three hours with measurements every two minutes using a fluorometric hydrogen peroxide assay kit (Sigma-Aldrich) and a Glomax microplate reader (Promega ...
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bioRxiv - Immunology 2021Quote: ... Liver triglyceride and total cholesterol concentrations were measured using commercial kits (all from Instruchemie) and expressed as nanomoles per milligram of total protein content using the Bradford protein assay kit (Sigma-Aldrich).
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bioRxiv - Immunology 2021Quote: Egg white immunoglobulins (300 μl) were purified using a commercially available protein A affinity chromatography kit (PURE-1A, Sigma Aldrich Co.). The manufacturer’s instructions were followed ...
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bioRxiv - Evolutionary Biology 2020Quote: ... GMA24F1A cells were cultured in 150 mm dishes and harvested for immunoprecipitation using the EZ-Magma ChIP HiSens kit (EMD Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... and plasma was immediately separated from peripheral blood mononuclear cells and polymorphonuclear leukocytes using a dual density gradient separation kit (Histopaque 1119/1077, Sigma-Aldrich), according to the manufacturer’s protocol ...