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Citations for Millipore Sigma :
6751 - 6800 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... RNA from young kiwifruit tissue culture leaves was extracted using the Spectrum Plant Total RNA kit (Sigma-Aldrich), following the manufacturer’s protocol except the incubation temperature of lysed tissue sample was increased to 65°C.
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bioRxiv - Biochemistry 2023Quote: ... CoA-SH released during the catalyzed reaction was monitored using the Coenzyme A Assay Kit (MAK034, Sigma-Aldrich). All assays were performed on a 200 μl scale in an IP buffer with 0.01% GDN ...
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bioRxiv - Cell Biology 2023Quote: Please note that most of the solutions listed are contained within the Duolink® PLA Kit (Sigma DUO92008). Upon receiving the PLA kit (Sigma DUO92008) ...
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bioRxiv - Microbiology 2023Quote: ... stained with PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane (Sigma-Aldrich, Saint Louis, Missouri, USA) according to manufacturer’s instructions and added at a concentration of 5×105 monocytes per model the day before adding epithelial cells.
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bioRxiv - Microbiology 2023Quote: ... under the control of the IPTG inducible T7 promoter using the pET-30 Ek/LIC Cloning Kit (Novagen). His/Strep-GlnA3Mt was synthesized in E ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Further extraction was done using RNA the Spectrum™ Plant Total RNA Kit (Sigma-Aldrich Chemie GmbH, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... Genomic DNA was extracted from ear slices with the GenElute Mammalian Genomic DNA Mini-prep Kit (Sigma-Aldrich). PCR was performed with PrimeSTAR® DNA Polymerase (Takara Bio ...
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bioRxiv - Bioengineering 2023Quote: ... an additional step of dialysis using Pur-A-LyzerTM Mini 6000 dialysis kit (PURN60030, Sigma-Aldrich, MO, USA) was added to remove iodixanol before grid preparation ...
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bioRxiv - Biochemistry 2023Quote: ATPase activity of FtsEX-EnvC was measured using a malachite green based colorimetric ATPase kit (MAK307, Sigma Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and myeloperoxidase (MPO) activity was determined using a Colorimetric Activity Assay Kit (Sigma-Aldrich, St. Louis, MO, USA), and the optical density of the reaction was measured at 412 nm in a micro-plate reader ...
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bioRxiv - Cell Biology 2023Quote: ... Isolated samples were homogenized and processed to evaluate hydroxyproline concentration using hydroxyproline assay kits (Sigma-Aldrich, Cat. # MAK008) following manufacturer’s instruction.
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bioRxiv - Neuroscience 2024Quote: The interaction of BRN1/2 with the indicated genes was studied by ChIP-qPCR in E14.5 wild-type brains using the ChIP assay kit following the manufacturer’s instructions (Millipore). Immunoprecipitation was performed with 5 µg of anti-BRN1/2 (PA5-30124 Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... the 6×His tag on the proteins was further cleaved with Thrombin CleanCleave kit (Sigma-Aldrich, Cat# RECMT) overnight at 4 °C on the rocking table after desaltig in 50 mM Tris-HCl ...
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bioRxiv - Bioengineering 2024Quote: Cathepsin B activity was assessed using the InnoZyme Cathepsin B Activity Assay Kit (MilliPore Sigma, catalog no. CBA001). Total cell lysates were extracted from CAR T cells using the provided cell lysis buffer according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ChIRP was performed in HeLa cells by using the Magna ChIRPTM RNA Interactome Kit (Sigma, cat #17-10494) strictly following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Cytoskeleton fraction characterization was performed using the ProteoExtract® Cytoskeleton Enrichment and Isolation Kit (Sigma-Aldrich, 17-10195) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: The blood phenylalanine levels were measured by an enzymatic method using the Phenylalanine Assay Kit (MAK005, Millipore Sigma) according to the manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... the QCM Chemotaxis 5µm 96-Well Cell Migration Assay Kit (Merck, Sigma-Aldrich, Darmstadt, Germany; Cat. No. ECM512). Human peripheral blood mononuclear cells (PBMCs ...
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bioRxiv - Plant Biology 2024Quote: ... Approximately 100mg of tissue was used for RNA extraction using Spectrum Plant Total RNA Kit (Sigma-Aldrich, USA) according to the manufacturer instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... toruloides strains cultured in the nitrogen-limited medium using the Spectrum Total RNA Kit (Sigma-Aldrich, Oakville, Canada). First-strand cDNA was synthesized with the SuperScript IV first-strand cDNA synthesis kit (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Triglyceride and free fatty acid levels were assessed using colorimetric kits (Cayman Chemicals #10010303 and Sigma-Aldrich #MAK044), respectively.
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bioRxiv - Neuroscience 2024Quote: ... antibodies were conjugated to plus-and minus-oligonucleotides using the Duolink PLA Probemaker kits (Sigma, #DUO92009 and #DUO92010) according to manufacturer’s proto-col ...
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bioRxiv - Developmental Biology 2024Quote: ... 14µm sections made with a Leica CM3050s cryostat were stained using a Masson Trichrome Staining Kit (Sigma-Aldrich). Manufacturer’s protocol was followed except for the omission of deparaffinization and microwaving ...
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bioRxiv - Cancer Biology 2021Quote: ... Counterstaining of nucleus was performed using Mayer’s hemalum solution and slides were mounted with Kaiser’s glycerol gelatin (the Kit and all reagents were obtained from Merck Millipore). Images were acquired as described above.
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bioRxiv - Developmental Biology 2021Quote: Cells were lysed with 0.5% SDS in EB buffer and bisulphite treated with the Imprint DNA Modification kit (Sigma). The resulting DNA was purified using columns and reagents from the EZ DNA Methylation Direct kit (Zymo Research) ...
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bioRxiv - Cell Biology 2019Quote: ... The membrane was blocked for 1 hour in 1% BSA/TBS-T and probed with antibodies provided in the kit according to the manufacturer’s instructions (Millipore).
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bioRxiv - Molecular Biology 2021Quote: ... 1/1000) antibodies for 2 h at 37 °C in antibody diluent solution (Duolink in situ kit, Sigma-Aldrich). The cells were then labeled with the PLA antimouse PLUS probe (DUO92001 ...
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bioRxiv - Molecular Biology 2021Quote: RNA extraction with on-column DNAse treatment were done using the GenElute Mammalian Total RNA Miniprep Kit (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA was isolated from the aboveground tissues of seedlings using the Spectrum Plant RNA Isolation Kit (Sigma-Aldrich). The RNA was treated with RNase-free RQ1 DNase (Promega) ...
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bioRxiv - Neuroscience 2021Quote: ... and performed western blotting analysis.Western blotting: Protein concentration of lysates was determined with Bicinchoninic Acid Kit (#BCA1, Sigma Aldrich). Equal amount of proteins was loaded on the poly-acrylamide gels ...
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bioRxiv - Neuroscience 2021Quote: ... collected by centrifugation and cell viability was determined by flow cytometry with Muse® Count &Viability Assay Kit (Millipore), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: Yolk sacs were dissected from embryos and used for DNA extraction with the Red Extract-N-Amp kit (Sigma). Usp9x status was assessed by PCR using Phire Green Hot Start II PCR Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... PLA and EdU counterstaining were performed according to the manufacturer’s instructions using the Duolink In Situ Red kit (Sigma) and goat anti-mouse Alexa Fluor 488 antibody.
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bioRxiv - Evolutionary Biology 2022Quote: ... We extracted DNA from our cultured microbiomes with GenElute Bacterial Genomic DNA kits (Millipore-Sigma, St. Louis, MO, USA). We sent ≈10 ng of DNA from each extraction to Genome Qúebec (McGill University ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We extracted DNA from our cultured microbiomes with GenElute Bacterial Genomic DNA kits (Millipore-Sigma, St. Louis, MO, USA). We sent ≈10 ng of DNA from each extraction to Genome Qúebec (McGill University ...
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bioRxiv - Molecular Biology 2019Quote: Whole-tissue sample lysates were batch processed for glycogen determination using commercially-available fluorometric kits (catalog #: MAK016; Millipore Sigma) as per the manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... and 30 min of working mode and LDH levels were measured using the TOX 7 assay kit (Sigma-Aldrich). Duplicate 75 μL aliquots were assayed according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... gDNA for illumina sequencing of the eleven other isolates was extracted using a Genelute Plant DNA Miniprep Kit (Sigma) using the manufacturers protocol with the following modifications ...
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bioRxiv - Evolutionary Biology 2020Quote: Total RNA was extracted from flower buds or leaves with the Spectrum Plant Total RNA kit (Sigma, Inc., USA), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... The protein band was excised from gel and digested with trypsin using trypsin profile IGD kit (Sigma-Aldrich, India). The peptides obtained after trypsin digestion were analyzed by Matrix-assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF ...
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bioRxiv - Plant Biology 2020Quote: ... France) and 500 μl of lysis/2-Mercaptoethanol solution of the SpectrumTM Plant Total μ RNA Kit (Sigma-Aldrich, St ...
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bioRxiv - Physiology 2019Quote: ... HDL-cholesterol and LDL-cholesterol concentration were evaluated enzymatically using assay kits (Sigma Chemical Co, St Louis, MO, USA). VLDL-cholesterol was calculated as triglycerides and LDL-cholesterol was calculated by the equation ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were subsequently fixed in 8% formaldehyde and stained using the Alkaline-Phosphatase kit (86R 1KT, Sigma-Aldrich, Germany) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: ... cells were collected at day 8 and RNA was extracted using GenElute Mammalian Total RNA MiniPrep Kit (Sigma #RTN350). cDNA was synthesized using oligodT primers using SuperScript II Reverse Transcriptase (Thermo Fisher Scientific #18064014) ...
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bioRxiv - Genetics 2020Quote: Blood ammonia levels were measured by ammonia colorimetric assay kits (BioVision Incorporated; Cat# K370-100 or Sigma; Cat#AA0100) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: In vitro PI3 Kinase activity measurements were performed with the PI3K Kinase Activity/Inhibitor assay kit following manufacturer’s instructions (Merck Millipore). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... PLA was performed according to the manufacturer’s recommendation (Duolink™ In Situ Red Starter Kit Mouse/Rabbit, Sigma-Aldrich). Primary antibodies against GFP (ab13970 ...
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bioRxiv - Neuroscience 2020Quote: Proteasome activity in mice brain tissue was analysed following the 20S Proteasome activity assay kit protocol by Millipore (APT280). The assay detects the fluorophore 7-Amino-4-methylcoumarin (AMC ...
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bioRxiv - Cancer Biology 2020Quote: ... an intracellular flow cytometry protocol was modified from the FlowCellect™ Autophagy LC3 Antibody-based Assay Kit (Millipore, FCCH100171). After RBC lysis ...
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bioRxiv - Neuroscience 2020Quote: PP2A activity was measured by using immunoprecipitation phosphatase assay kit according to the manufacturer’s instructions (Catalog # 17-313, Millipore). Statistical differences were analyzed using post hoc test with Bonferroni’s correction following one-way ANOVA ...