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Citations for Millipore Sigma :
7001 - 7050 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... Hybridization was performed at 50°C O/N with the probe 1.6 kb fragment containing the sequence (TTAGGG)n63 labelled with Nick Translation Kit (Sigma-Aldrich) and developed according to62 ...
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bioRxiv - Microbiology 2019Quote: ... Total cellular phospholipid was measured in the samples prepared for the cholesterol assay using a phospholipid assay kit (MAK122, Sigma). Cholesterol concentrations were then normalized to phospholipid concentrations.
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bioRxiv - Neuroscience 2019Quote: Fish larvae at 5dpf were fixed in 4% PFA and the TUNEL assay was performed according to standard protocol using ApopTag Kit (Millipore). Briefly ...
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bioRxiv - Plant Biology 2019Quote: Glucose concentration of the spent media was determined spectrophotometrically with the commercial High Sensitivity Glucose Assay Kit (Sigma-Aldrich, U.S.). Prior to measurements ...
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bioRxiv - Molecular Biology 2019Quote: ... The percentage of immunoprecipitate (IP) to Input was calculated as described in the Magna MeRIPTM m6A kit (17-10499, Millipore). mRNA controls (m6A RNA and unmodified RNA ...
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bioRxiv - Pathology 2019Quote: ... treated with opsonized RBCs (1:10) and intracellular ROS was determined with a fluorometric intracellular ROS kit (Sigma-Aldrich, MAK142). Stained cells were visualized and captured using Leica DMI6000B with Cy5 filter set ...
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bioRxiv - Genetics 2021Quote: ... All plasmids to be used for transfection were purified with HP GenElute Midi or Mini kits (Sigma # NA0200 and NA0150).
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bioRxiv - Immunology 2021Quote: ... was used for m6A immunoprecipitation (m6A-IP) using an m6A antibody (Synaptic, 202003) following the standard protocol of the Magna MeRIP m6A Kit (Merck Millipore). RNA was enriched through RNA Clean & Concentration-5 (Zymo Research ...
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bioRxiv - Genetics 2020Quote: ... Enrichment of the chloroplast from the leaf samples was performed using ± 2 cm2 of leaf tissue with the Chloroplast Isolation Kit (Sigma, CPISO-1KT After this the DNA was extracted using a modified C-Tab protocol (Doyle JJ ...
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bioRxiv - Cancer Biology 2020Quote: ... and DNA was extracted as per the protocol in the Actetyl-Histone H3 Immunoprecipitation (ChIP) assay kit (Millipore, Billerica, MA). Anti-acetyl-Histone H3 antibody was used for the immunoprecipitation (Millipore) ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: Insulin and glucose were measured in plasma samples using the Sensitive Insulin enzyme immunoassay kit (EZRMI-13K, Millipore, Billerica, MA) and Glucose GOD-PAP enzyme immunoassay kit (Roche Diagnostics ...
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bioRxiv - Microbiology 2021Quote: ... RNA was extracted as per manufacturer instruction and genomic DNA was removed using the RNase-free DNase I Amplification Grade Kit (Sigma). All RNA samples were assessed for quality using an Agilent 2100 Bioanalyzer ...
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bioRxiv - Cell Biology 2021Quote: Proximity ligation assay (PLA) was performed using the Duolink® In Situ Red Mouse/Rabbit kit (DUO92008, Sigma-Aldrich, UK) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: Both the ADP/ATP ratio and the total cellular ATP content were measured using a bioluminescence-based ADP/ATP assay kit (Sigma) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... DNA oligonucleotides and the GenElute HP Plasmid Miniprep and Midiprep kit used for plasmid purifications were acquired from Sigma-Aldrich. Q5 High-Fidelity DNA Polymerase ...
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bioRxiv - Biochemistry 2020Quote: ... revVS and shVS were purified from cultures of transformed Escherichia coli C41(DE3) cells that were induced with the Overnight Express Autoinduction Kit System 1 (Novagen). Cells were pelleted and resuspended in Buffer A containing 10mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: The phosphatase activity of purified PP2A/B55α complexes was assessed using a commercial Ser/Thr phosphatase assay kit (EMD Millipore). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and cortex was determined following the standard Ellman reaction principle using a commercially available AChE activity assay kit (Catalog no. MAK119, Sigma-Millipore). The Ellman assay [42] was used to measure ChE activity in each brain region using 5,5’-dithio-bis (2-nitrobenzoic acid ...
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bioRxiv - Bioengineering 2021Quote: ... the pellets were collected to examine the cell lysis interference using a Glucose-6-Phosphate Dehydrogenase kit (Sigma-Aldrich, USA). The details of EPS extraction and analytical methods for various EPS components (proteins ...
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bioRxiv - Cell Biology 2021Quote: ... G6P concentrations were determined at 2 hrs and 6 hrs post activation and compared to unactivated controls using a G6P kit (Sigma) and following manufacturer’s instructions.
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Interactions with stromal cells promote a more oxidized cancer cell redox state in pancreatic tumorsbioRxiv - Cancer Biology 2020Quote: ... R-spondin was purified from 293T cells engineered to produce it using a Protein A Antibody Purification Kit (Sigma PURE1A). For PDAC cells grown as 3D organoids in co-culture ...
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bioRxiv - Cancer Biology 2020Quote: ... R-spondin was purified from 293T cells engineered to produce it using a Protein A Antibody Purification Kit (Sigma PURE1A). Organoids were grown in complete media when passaging ...
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bioRxiv - Cancer Biology 2019Quote: ... Murine mCherry-G166 GSCs glioma cells or human G166 GSCs labelled with a Red Fluorescent Cell kit PKH26 (Sigma-Aldrich) were injected into NOD SCID mice ...
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bioRxiv - Cell Biology 2021Quote: ... The PKH26:MYH10-KD co-cultures 3T3-L1 cells were labeled with 0.5λ PKH26 Fluorescent Cell Linker Kit (Sigma-Aldrich); the GFP cells were added after one day.
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bioRxiv - Cell Biology 2021Quote: ... and RANK-L (100 ng/ml) for 4 to 6 days followed by staining for Tartrate-resistant acid phosphatase (TRAP) using a kit (Sigma) per manufacturer’s instruction ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were washed once in PBS and nuclei obtained using the EZ Prep nuclei kit then incubated with 50μM Azide-PEG3-biotin conjugate (Sigma-Aldrich) was dissolved in prewarmed Buffer A and added to samples. ...
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bioRxiv - Biochemistry 2020Quote: ... The cells and media were separated after the incubation and processed for quantification of insulin levels using the RI-13K Rat Insulin RIA Kit (Millipore) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2021Quote: Immunoprecipitation was performed using SRF or MKL1 antibody or control rabbit IgG with Magnetic Protein A/G beads (Magna ChIP A/G kit, Millipore), as described previously (36) ...
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bioRxiv - Microbiology 2020Quote: ... The immunoprecipitates were applied to 8 % SDS-PAGE and the gel was stained by ProteoSilver™ stain kit (Sigma-Aldrich) according to manufacturer’s instruction ...
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bioRxiv - Plant Biology 2020Quote: ... RNA was extracted from the in vitro samples using the Spectrum™ Plant Total RNA kit (Sigma-Aldrich, MO, USA) according to the manufacturers’ instructions ...
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bioRxiv - Genomics 2021Quote: The high-quality genomic DNA was isolated from all three strains using GenElute™ Bacterial Genomic DNA Kit (Sigma-Aldrich) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Bacterial expression vectors for human Arf1 (UniProtKB: P84077) and Arf6 (UniProtKB: P62330) were constructed using a pET-41 Ek/LIC vector kit (Novagen), as described for protein expression of human Rab-family small GTPases (Tamura and Mima ...
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bioRxiv - Microbiology 2021Quote: 5′RACE (Rapid amplification of cDNA ends) assays were performed with total RNA extracted from UK1_O using a 5′RACE System kit (Sigma Aldrich), according to the instruction provided by the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... The enzymatic activity of DHFR with and without SCH-79797 treatment was measured using the Dihydrofolate Reductase Assay kit from Sigma. Briefly ...
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bioRxiv - Microbiology 2020Quote: ... transferred to DMEM (1000 mg/l) and media glucose concentration was measured over a 24 h period using a glucose assay kit (Sigma). Glucose levels were expressed relative to media collected from worms which received naïve IgG (negative control) ...
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bioRxiv - Cancer Biology 2020Quote: ... The resulting plasma supernatant was transferred to a sterile Eppendorf tube and stored at −80 °C until the cytokines were measured using a Milliplex xMAP Mouse High Sensitivity TCell Magnetic Bead Panel kit MHSTCMAG-70K (Millipore) following the kit protocol on a Luminex 200 system with Xponent (v2.7) ...
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bioRxiv - Cancer Biology 2020Quote: ... Whole genome amplification was performed using SeqPlex™ Enhanced DNA Amplification kit as described by the manufacturer (Sigma-Aldrich, SEQXE). Amplified DNA was purified using PCR purification product kit as described by the manufacturer (Macherey-Nagel ...
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bioRxiv - Cell Biology 2021Quote: ... Cardiac and H9c2 ATP content was determined using a the Adenosine 5′-triphosphate (ATP) Bioluminescent Assay Kit (Sigma #FLAA-1KT), and normalized to DNA content as described previously 50 ...
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bioRxiv - Cell Biology 2021Quote: ... and lactate and pyruvate were measured using colorimetric assays using kits per manufacturer’s instructions (insulin, Cat no. RI-13K, Millipore Sigma, Burlington ...
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bioRxiv - Cancer Biology 2021Quote: ... was used to determine the concentration of harvested cell lysates and 500 μg of protein lysate was loaded into the supplied spin column (Catch and Release Immunoprecipitation Kit; Millipore). Immunoprecipitation using antibodies against FLAG (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2022Quote: ... pre-sorted or sorted cells were stained with either trypan blue or a Calbiochem live/dead double staining kit (Millepore Sigma) which uses a cell permeable green fluorescent Cyto-dye to stain live cells and propidium iodine to stain dead cells ...
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bioRxiv - Cancer Biology 2022Quote: Oleoyl-L-α-lysophosphatidic acid (LPA, L7260) and a Periodic Acid-Schiff (PAS) Kit (395B) were purchased from Sigma-Aldrich. The PKD inhibitor CRT0066101 (A8679 ...
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bioRxiv - Physiology 2022Quote: Cellular ATP levels in FACS-sorted RPMs (10 000 cells/sample) were determined using ATP Fluorometric Assay Kit (Sigma, MAK190), as per the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... sensitivity calibration and standard curves were made on the basis of recommendations of the 20S Proteasome Activity Assay Kit (Millipore).
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bioRxiv - Plant Biology 2022Quote: ... total RNA was extracted from 14 day-old Arabidopsis thaliana seedlings using the SpectrumTM Plant Total RNA Kit (Sigma-Aldrich) and treated with DNAase I (RapidOut DNA Removal Kit ...
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bioRxiv - Plant Biology 2022Quote: ... thaliana seedlings for each line grown on a ½ MS agar plate using the SpectrumTM Plant Total RNA Kit (Sigma-Aldrich) following the manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: ... resuspended in RNA extract lysis buffer (100µL RLT lysis buffer from RNA extraction kit supplemented with 40mM DTT, Sigma, #D9779), vortexed and let sit (10min. ...
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bioRxiv - Neuroscience 2022Quote: The MILLIPLEX® MAP human amyloid beta and tau magnetic bead panel 4-plex ELISA kit (Millipore, Burlington, MA, USA) and the Bio-Plex 200 System instrument (Biorad ...
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bioRxiv - Neuroscience 2022Quote: ... Cat# AS1107) for one hour at room temperature and visualized using the Immobilon ECL substrate kit (Millipore, Merck KgaA, Germany). Primary antibodies specific to PLLP (1:700 ...
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bioRxiv - Plant Biology 2022Quote: ... obtained by grinding in liquid nitrogen and using a SpectrumTM Plant Total RNA kit (Sigma-Aldrich, Merck KGaA, Darmstadt, DE) according to the manufacturer’s instructions ...