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Citations for Millipore Sigma :
6851 - 6900 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: ... 200 μL of incubation media was removed and kept to measure glycerol and FA release using commercial kits (Sigma-F6428 and Wako diagnostic NEFA-HR ...
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Potential of host serum protein biosignatures in the diagnosis of tuberculous meningitis in childrenbioRxiv - Immunology 2019Quote: All biomarkers were evaluated following the instructions of the respective kit manufacturers (R&D Systems and Merck Millipore, respectively) in a blinded manner ...
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bioRxiv - Neuroscience 2019Quote: DNA from offspring was extracted from mouse ear punches using Extract-N-Amp® kit (Sigma, St. Louis, MO) and genotyped using a multiplex PCR reaction ...
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bioRxiv - Biochemistry 2019Quote: ... the levels were measured using the Single Molecule Counting (SMC®) Immunoassay Technology using commercially available kits from Millipore / Sigma (Aβ1-40 ...
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bioRxiv - Cancer Biology 2019Quote: ... The proximity ligation assay was performed using a Duolink In Situ Red Starter Kit Mouse/Rabbit (Sigma-Aldrich DUO92101). The cells were blocked with a solution provided by the kit and then incubated overnight at 4°C in a 1:500 dilution of primary antibodies for p53 and Parkin ...
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bioRxiv - Cell Biology 2020Quote: Mitochondria were freshly isolated from IECs of Smyd5fl/fl and Smyd5ΔIEC mice as described before 20 using a mitochondria isolation kit (MITOISO1, Sigma) according to the manufacturer’s instruction.
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bioRxiv - Microbiology 2019Quote: ... Viral nucleotides were randomly amplified using a modified version of the Whole Transcriptome Amplification Kit 2 (WTA2, Sigma Aldrich) as described in Conceicao-Neto ...
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bioRxiv - Genetics 2020Quote: Purification of fatty acids was performed using the fatty acids purification kit cat MAK174 (Sigma Aldrich, St Louis, MO) from 0,15 grams of BFT flour ...
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bioRxiv - Cell Biology 2020Quote: ... 100 ul culture media was collected and acetate level was quantified with Acetate Colorimetric Assay Kit (#MAK086-1KT, SIGMA) following the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2021Quote: Human genomic DNA was isolated from HeLa cells using a GenElute™ Mammalian Genomic DNA Miniprep Kit (Sigma-Aldrich). Target genes were PCR amplified from genomic DNA using Phusion® High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Microbiology 2020Quote: ... Cleared lysates were obtained after centrifugation for 1 hour at 20,000 x g and incubated with anti-FLAG M2 affinity gel (FLAG Immunoprecipitation Kit, Sigma). Bound proteins were eluted with 3xFLAG peptide solution.
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bioRxiv - Microbiology 2020Quote: ... Cell pellets were resuspended in P1 solution (accordingly to kit mannufacturer) and incubated with with 9U lyticase (Sigma-Aldrich) at 37 °C for 30 minutes ...
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bioRxiv - Cancer Biology 2019Quote: ... and the supernatant was tested for total protein estimate using Total Protein kit from Millipore Sigma (see material section) and cell lysate was frozen at −70°C for electrophoresis where 10µg or 20 µg per sample in sample buffer was heated up at 95°C for 5 min and run in Tris-Glycine gel followed by dry transfer for Western blot detection following the instruction for using iBlot® and/or iBlot®2 Dry Blotting with Blot®2 NC Regular Stacks.
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bioRxiv - Microbiology 2019Quote: ... viral RNA (vRNA) was extracted from 100 µl of supernatant using the GenElute mammalian total RNA kit (Sigma-Aldrich), according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2019Quote: ... Plasmid DNA was purified with a NucleoBond Xtra Midi kit (Cultech, 22740410.50) and resuspended in nuclease-free water (SIGMA). Other plasmids used were pCMV-GFP expressing GFP under the CMV promoter ...
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bioRxiv - Genetics 2021Quote: ... These kits were used to purify DNA from crude lysates or from tissue samples preserved in RNAlater solution (Sigma).
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bioRxiv - Molecular Biology 2021Quote: RNA immunoprecipitation (RIP) experiments were performed using the Magna RIPTM RNA-Binding Protein Immunoprecipitation Kit (17-700, Millipore, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: The ATPase activity of MutL or MutL(R-E) was measured by an ATPase/GTPase Activity Assay Kit (Sigma). The analysis was carried out with 5 μM protein in a 40 μL reaction mixture comprised of 20 mM Tris-HCl (pH 7.8) ...
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bioRxiv - Cell Biology 2020Quote: ... The quantity of glucose produced was then measured using a Glucose assay Kit (Sigma-Aldrich, St. Louis, MO; #GAGO20). For all assays ...
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bioRxiv - Immunology 2020Quote: DHFR activity of the recombinant β-lactamase-DHFR fusion protein was evaluated using the DHFR Assay Kit (Sigma-Aldrich), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... LPS (Escherichia coli serotype 0111: B4) and Acid phosphatase leukocyte (TRAP) kit (387A) were procured from Sigma-Aldrich (USA). Macrophage-colony stimulating factor (M-CSF ...
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bioRxiv - Cell Biology 2020Quote: ... Subsequently the PLA protocol was followed according to the Duolink In Situ Red Starter Kit Mouse/Rabbit (Millipore Sigma) instructions ...
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bioRxiv - Genetics 2019Quote: ... centrifuged 20 min at 20000 g and recombinant GST-tagged proteins were purified using the GST-BindTM Kit (Novagen). To synthetize the NOVA2 RNA target ...
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bioRxiv - Neuroscience 2021Quote: ... or Gad1 were generated by in vitro transcription using cDNA templates and RNA DIG- or FITC-labeling kits (Sigma, catalog # 11277073910 or 11685619910 ...
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Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: Binding of cGAS and dsDNA in human and mouse brain sections was determined by PLA kit (DUO92101, Sigma-Aldrich) accordingly to the manufacture’s instruction ...
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bioRxiv - Developmental Biology 2022Quote: Nuclei from the 700mg of wildtype flower tissue were isolated using the Plant Nuclei isolation kit (Sigma, # CELLYTPN 1) conferring to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μL of each sample was used as input for the NAD/NADH Quantitation Kit (Sigma-Aldrich, cat# MAK037) according to the instructions provided by the manufacturer ...
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bioRxiv - Cell Biology 2022Quote: ... Chromatin shearing and immunoprecipitation were performed using EZ-Magna ChIP A/G Chromatin Immunoprecipitation Kit (Sigma, St. Louis, MO) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: Mitochondrial mass in whole liver was measured according to the manufacturer’s instructions using the Citrate Synthase Activity Assay Kit (Sigma) and normalized to protein concentration ...
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bioRxiv - Cancer Biology 2022Quote: Nuclei were isolated from 50,000 cells for each sample using Nuclei EZ prep-Nuclei Isolation Kit (Sigma-Aldrich, USA). The transposition reaction mix (25μl of 2X TD buffer ...
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bioRxiv - Bioengineering 2022Quote: ... the lipid oxidation and superoxide level assays were conducted according to manufacturer’s specifications (Lipid peroxidation assay kit, Sigma Aldrich Cat#MAK085 ...
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bioRxiv - Cell Biology 2022Quote: Total hydroxyproline content to quantify extracellular matrix deposited collagen was measured using a commercially available kit (#MAK008, Sigma-Aldrich) as previously detailed125 ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were fixed and stained with Tartrate-resistant acid phosphatase (TRAP) kit according to the manufacturer’s instructions (Sigma) to quantify osteoclast numbers ...
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bioRxiv - Genetics 2022Quote: ... Media aliquots were frozen and subjected to ENG ELISA analysis with the Human Endoglin ELISA kit (Sigma-Aldrich RAB0171) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... stems and roots of M15 plants grown outside under low light conditions using SpectrumTM Plant Total RNA Kit (Sigma). For differential gene expression analyses of secondary metabolite genes ...
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bioRxiv - Molecular Biology 2022Quote: Duolink™ proximity ligation assays were performed with the Duolink In Situ Red Starter Kit Mouse/Goat (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Plasmid library DNA was purified from twice 5 ml of culture using a GenElute Plasmid Miniprep Kit (Sigma-Aldrich) with elution in twice 20 µl of ddH2O ...
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bioRxiv - Microbiology 2022Quote: ... Size standards were generated using a gel filtration marker kit of protein molecular weights that ranges from 29,000 to 700,000 (Sigma-Aldrich).
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bioRxiv - Neuroscience 2022Quote: We measured cytokine concentration in maternal and fetal plasma by Luminex technology using multiplex kits for nonhuman primate (Millipore). Concentrations were calculated from standard curves for recombinant proteins.
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bioRxiv - Molecular Biology 2023Quote: Drug efflux by MDR1 was measured with the Multidrug Resistance Direct Dye Efflux Assay kit (Merck Millipore; ECM 910) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... The WTA product was performed using the Complete Whole Transcriptome Amplification Kit (WTA2)39 (Sigma-Aldrich, St. Louis, MO), with the PCR reaction then undertaken using a set of self-designed primer pairs based on the obtained reads ...
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bioRxiv - Physiology 2022Quote: Senescence-associated β-gal staining was performed with the Cellular Senescence Assay Kit according to the manufacturer’s instructions (Millipore).
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bioRxiv - Plant Biology 2023Quote: RNA extraction of Marchantia polymorpha and Physcomitrium patens was done using the Spectrum™ Plant Total RNA Kit (Sigma) and for Arabidopsis thaliana the NucleoSpin RNA Plant min kit (Macherey -Nagel ...
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bioRxiv - Molecular Biology 2022Quote: The content of hydroxyproline in ligament fibroblasts was measured using a hydrolysate of hydroxyproline assay kit (Sigma, MO, USA) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... and either lysed with RNA lysis buffer or fixed and stained using an Oil-red-O staining kit (Sigma) as per manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Free (non-esterified) fatty acids were measured using Roche’s Free Fatty Acid Kit (half-micro test) (Sigma Aldrich – 11383175001), which is based on the enzymatic conversion of free fatty acids to acyl CoA by acyl-Co A synthetase.
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bioRxiv - Neuroscience 2022Quote: ... and Pv were generated by in vitro transcription using cDNA templates and RNA DIG-or FITC-labeling kits (Sigma, catalog # 11277073910 or 11685619910 ...
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bioRxiv - Immunology 2022Quote: ... cell corpses were stained with the PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane Labelling (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: The cells were lysed with radio-immunoprecipitation assay (RIPA) buffer (Nacalai tesque) or ProteoExtract Subcellular Proteome Extraction Kit (Millipore) containing a protease inhibitor cocktail (Nacalai tesque) ...
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bioRxiv - Cancer Biology 2024Quote: ... ND-Methyladenosine RNA immunoprecipitation (meRIP) was performed using Magna MeRIP™ m6A Kit (Cat# 17-10499, Sigma-Aldrich, UK) as per manufacturer’s instructions ...