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Citations for Millipore Sigma :
6901 - 6950 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and supernatant of the culture medium was collected and examined using a glucose determination commercial kit (Sigma-Aldrich, USA). For fatty acid uptake 38 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 75 μl PKH26 dye (0.5 μM, PKH26 Red Fluorescent Cell Linker Kit for Phagocytic Cell Labeling, Sigma-Aldrich, Germany) was applied at least 5 days before imaging via oropharyngeal application ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and the nuclear/cytoplasmic cell fractionation Cell Lytic™ NuCLEAR™ extraction kit were from Sigma (St. Louis, MO). Sequencing grade trypsin was purchased from Promega (Madison ...
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bioRxiv - Physiology 2023Quote: ... Total starch content was then quantified with a commercial starch kit (Amylase/Amyloglucosidase Method - Product Code STA-20, SIGMA) and a spectrometer following the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: The ATP content in LV or CM was measured using the ATP Assay Kit (Sigma-Aldrich; catalog number: MAK190) following the manufacturer’s instructions and using the Synergy HT spectrophotometer (Biotek) ...
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bioRxiv - Cell Biology 2024Quote: The PLA was performed using the Duolink In Situ labeling kit reagents according to the manufactureŕs protocol (Sigma Aldrich). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: Protein-protein interaction was measured by PLA with Duolink In Situ Orange Starter Kit Mouse/Rabbit (DUO92102, Millipore Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Samples of culture were adjusted to OD600 0.5 mL−1 and processed according to the manufacturer’s instruction ADP/ATP Ratio Assay Kit (Sigma-Aldrich). For NADH/NAD+ quantification samples from WT and ΔmftG grown in triplicates on HdB-Tyl supplemented with 10 g L−1 glucose for 24h were also quantified ...
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bioRxiv - Microbiology 2024Quote: ... The resulting extracts were treated and measured according to the NAD+/NADH Assay Kit manufactures’ protocol (MAK460, Sigma-Aldrich). The readings of each plate were acquired using a CLARIOStar microplate reader (BMG Labtech ...
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bioRxiv - Immunology 2024Quote: Hydroxyproline was measured using 10 mg of lung tissue using a hydroxyproline assay kit (Sigma-Aldrich, Cat. No. MAK008) per the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: Cell viability assay was performed using the cell proliferation reagent Cell Counting Kit-8 (CCK-8) following the manufacturer’s protocol (Sigma). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... Whole genome amplification was performed using SeqPlextm Enhanced DNA Amplification kit as described by the manufacturer (Sigma-Aldrich, SEQXE). Amplified DNA was purified using PCR purification product kit as described by the manufacturer (Macherey-Nagel ...
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bioRxiv - Immunology 2023Quote: ... 2 and 3 were detected with another multiplex assay (MILLIPLEX MAP TGFß Magnetic Bead 3 Plex Kit; Merck Millipore).
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bioRxiv - Pathology 2023Quote: Concentration of APLN in the medium and in mouse plasma was determined using APLN EIA Kit (RAB0018, Sigma-aldrich) using the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2022Quote: ... performed twice with sample rotation between runs) and total RNA was extracted (Spectrum™ Plant Total RNA Kit, Sigma). Technical replicates were pooled ...
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bioRxiv - Biochemistry 2022Quote: ... The concentration of PO4 in the samples was analyzed using the Malachite Green Phosphate Assay Kit from Sigma-Aldrich. This was done in a new 96-well plate by mixing 72 µl water with 8 µl of the samples ...
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bioRxiv - Immunology 2023Quote: ... the reaction mixture was incubated at 37°C for 1h and its aconitase activity was determined per manufacturer’s instructions (Aconitase Assay Kit from Sigma).
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bioRxiv - Pathology 2023Quote: ... 10um sections every 20th section from ligature to apex through the heart were stained using the Masson’s Trichrome Stain Kit (Sigma) and imaged using an Aperio slide scanner ...
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bioRxiv - Molecular Biology 2023Quote: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium Bromide (MTT) cell growth kit (CT02, Millipore, MA, USA) was used for measuring cell viability ...
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bioRxiv - Physiology 2023Quote: Total HDAC activity was assessed with the in situ HDAC activity fluorometric assay kit (Sigma Aldrich, St. Louis, MO). After isolation ...
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bioRxiv - Cell Biology 2023Quote: ... followed by ligation and amplification according to the manufacturer’s protocol (Duolink® In situ Red Starter kit, Sigma-Aldrich). The embryos were then mounted in ProLongTM Diamond Antifade Mountant with DAPI (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... retinoic acid and epidermal growth factor (EGF) as per the SAGM Bullet Kit and 0.1 μg ml−1 cholera toxin (cat. no. C8052, Sigma), 5% FBS and 1% Pen/Strep ...
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bioRxiv - Cancer Biology 2023Quote: ... and genomic amplification reactions were done with the GenomePlex® Single Cell Whole Genome Amplification Kit (Millipore Sigma, WGA4). Initial genomic library distributions were assessed on a 1.5% agarose gel and purified using a ZR-96 Genomic DNA Clean & Concentrator-5 Kit (Zymo Research ...
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bioRxiv - Bioengineering 2023Quote: ... The electroporated mixture was transferred into a 1.5 mL tube to which is added PKH26 dye (0.4 µL) (PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane Labeling, Sigma) mixed with 100 µL diluent C ...
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bioRxiv - Cancer Biology 2023Quote: ... with squamous cell carcinomas and 38 female age-matched controls (mean age of 50.4 ± 9.6) the Succinate Colorimetric Assay Kit (Sigma Aldrich) was used according to the manufacturers′ instruction.
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bioRxiv - Bioengineering 2023Quote: Cell viability of various materials were determined using CCK-8 assay (Sigma Cell Counting Kit-8, BCCB8166, Sigma Aldrich). SH-SY5Y cells were seeded in 96-well plates at a density of 5,000 cells per well ...
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bioRxiv - Cancer Biology 2023Quote: ... Fixed cells were transposed using barcoded Tn5 (Seqwell) in a transposition buffer (1 × TD buffer from Illumina Nextera kit, 0.1% Tween 20 (P9416, Sigma), 0.01% Digitonin (G9441 ...
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bioRxiv - Cancer Biology 2023Quote: ... USA) with the mouse Tumor Dissociation kit (Miltenui Biotec) and lymphocytes were enriched on a Ficoll gradient (Sigma-Aldrich). T cells were stained using anti-CD3-Alexa Fluor 488 ...
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bioRxiv - Biochemistry 2023Quote: ... hellmanex III and the Pur-A-Lyzert dialysis kit (molecular weight cutoff 3.5 kDa) were purchased from Sigma-Aldrich. Sodium dihydrogen phosphate monohydrate (NaH2PO4.H2O) ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were purified using Amicon Pro Affinity Concentration Kit Protein A with 10kDa Amicon Ultra-0.5 Device (Millipore-Sigma). Antibody concentrations were determined using an IgG ELISA ...
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bioRxiv - Plant Biology 2023Quote: ... the nuclei enrichment and isolation was performed on fresh leaf material using CelLytic PN Isolation/Extraction Kit (Sigma-Aldrich). DNA was extracted from plant cells and Hi-C libraries were prepared using EpiTect Hi-C Kit (Qiagen ...
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bioRxiv - Immunology 2023Quote: ... These oligonucleotides were used to create a phage display library using the T7Select 415-1 Cloning Kit (Merck Millipore). Immunoprecipitation of the library was performed in accordance with a previously published protocol7,11 ...
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bioRxiv - Cancer Biology 2023Quote: ... The proximity ligation assay (PLA) was conducted with Duolink® In Situ Red Starter Kit Mouse/Rabbit (DUO92101, Sigma) following the standard protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... that were cultured and prepared using a GenElute HP Plasmid Midi kit (NA0200-1KT, Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Biochemistry 2023Quote: ... Muse® cell analyzer kits for measurement of caspase3/7 (MCH100108) and cell cycle (MCH100106) were from EMD Millipore. Unless otherwise noted ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were manually scraped off plates to perform a plasmid DNA purification using GenElute Megaprep kit (NA0600-1KT, Sigma).
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bioRxiv - Evolutionary Biology 2023Quote: ... RNA isolations were performed using the Sigma SpectrumTM Plant Total RNA extraction kit (Sigma-Aldrich, St. Louis, MO, USA); Polyclar AT (Serva ...
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bioRxiv - Physiology 2023Quote: ... Apoptosis was assessed by terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining following manufacturer’s instructions (Millipore S7110 kit). Infarct size after LAD ligation was measured by Evans blue dye (1% ...
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Variation of wine preference amongst consumers is influenced by the composition of salivary proteinsbioRxiv - Biochemistry 2023Quote: ... α-Amylase activity assay was carried out using an amylase activity assay kit following the user manual (Sigma-Aldrich). Total salivary esterase activity was tested following María ...
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bioRxiv - Bioengineering 2023Quote: Glucose and xylose concentrations in culture supernatants were alternatively determined also by Glucose (GO) Assay Kit (Sigma-Aldrich, USA) and Xylose Assay Kit (Megazyme ...
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Machine Learning Ensemble Directed Engineering of Genetically Encoded Fluorescent Calcium IndicatorsbioRxiv - Bioengineering 2023Quote: Isolated primary cortical neurons were transfected using the calcium phosphate transfection kit from Sigma Aldrich (Sigma-Aldrich; CAPHOS-1KT). Half of the neuron media was changed 24 hours before transfection ...
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Machine Learning Ensemble Directed Engineering of Genetically Encoded Fluorescent Calcium IndicatorsbioRxiv - Bioengineering 2023Quote: Isolated primary cortical neurons were transfected using the calcium phosphate transfection kit from Sigma Aldrich (Sigma-Aldrich; CAPHOS-1KT). Half of the neuron media was changed 24 hours before transfection ...
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bioRxiv - Pathology 2023Quote: ... Masson’s trichrome staining was performed by using a commercially available stain kit (HT15, Sigma-Aldrich, St. Louis, MA, USA).
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bioRxiv - Neuroscience 2022Quote: The serum lactate from cerebral ischemia stroke patients and healthy volunteers was measured by the Lactate Assay Kit (Sigma) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... cell pluripotency was evaluated by assessing alkaline phosphatase (AP) activity using the leukocyte alkaline phosphatase kit (Sigma, 86R-1KT) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... We omitted the fragmentation step and amplified the samples for 15 rounds using the GenomePlex WGA Reamplification Kit (Sigma). We purified the samples using the QIAquick PCR Purification Kit and eluted them in 30 uL ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were purified using Amicon Pro Affinity Concentration Kit Protein A with 10kDa Amicon Ultra-0.5 Device (Millipore-Sigma). Antibody concentrations were determined using an IgG ELISA ...
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Autorepression of Yeast Hsp70 co-chaperones by intramolecular interactions involving their J-domainsbioRxiv - Biochemistry 2024Quote: Colorimetric determination of Pi produced by ATP hydrolysis was performed using the Malachite Green Assay Kit (Sigma-Aldrich, Switzerland) and as described previously (Lee ...
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bioRxiv - Plant Biology 2024Quote: Genomic DNA was extracted from a small piece of Arabidopsis leaf using Extract-N-Amp extraction kit (Sigma-Aldrich) and used as a template for PCR ...
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bioRxiv - Molecular Biology 2023Quote: PLA experiments were performed using the DuoLink In Situ Red Starter Kit Mouse/Rabbit (Millipore/Sigma-Aldrich # DUO92101-1KT) and following manufacturer protocol ...