Labshake search
Citations for Millipore Sigma :
7151 - 7200 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... free fatty acid (C8 and longer) and glycerol concentrations were assessed using commercially available colorimetric kits (MAK044 and MAK117, Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Anti-PP1α antibody (2 μg) was added to the supplied spin column (Catch and Release Immunoprecipitation Kit; cat no. 17-500; Millipore) along with the cell lysates (500 mg ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA from sucrose gradient fractions with heparin (55 u/ml) were extracted with the RNA Purification kit (Sigma RNB100-50RXN). Northern blots were performed as described elsewhere45 ...
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bioRxiv - Cell Biology 2022Quote: NALM6 target cells were labelled with CellVue membrane dye (CellVue Claret Far Red Fluorescent Cell Linker Kit protocol; Sigma-Aldrich) and 6.25 × 104 or 1.25 × 105 cytotoxic CD8+ T cells were used at indicated effector to target rations ...
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bioRxiv - Plant Biology 2024Quote: ... Seedlings were flash-frozen in liquid nitrogen before total RNA was extracted using SpectrumTM Plant Total RNA Kit (SIGMA Aldrich).
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bioRxiv - Biochemistry 2024Quote: ... blood plasma was collected at 9 am and ELISA kits were used following manufacturer’s instructions (Leptin: Crystal Chem, 90030; GLP-1: Sigma, EZGLP1T-36K ...
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bioRxiv - Biochemistry 2024Quote: ... Cell viability under 42 h compound treatment was determined via the Cell Counting Kit-8 (CCK-8, Sigma-Aldrich #96992) following the manufacturer’s instructions.
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bioRxiv - Biochemistry 2024Quote: ... Genomic DNA was extracted from single colonies after culturing in TYG using the GenElute™ Bacterial Genomic DNA kit (Sigma) and screened for tag insertions by PCR using primers flanking various att sites ...
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bioRxiv - Neuroscience 2024Quote: ... IL-10 and TNF-α were quantified in the FN of NL and at 21dpi aged GFAP-IL6Tg and WT mice (n= 4-6/experimental group) using a Milliplex MAP Mouse High Sensitivity kit (#MHSTCMAG-70K; Merck Millipore), following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... The remaining iron nitrate salt was allowed to cool then quantified using a colorimetric iron assay kit with 3 technical replicates for each cell type (MAK025, Sigma). The total iron from 3 technical replicates was averaged then divided by the number of cells to obtain calculated pg Fe/cell.
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bioRxiv - Physiology 2024Quote: Collagen content was analyzed by determination of hydroxyproline levels in left ventricles or in cell homogenates using the Hydroxyproline Assay kit (MAK008, Sigma) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ELISAs were used to confirm a subset of cytokines identified in the antibody arrays (CCL2, CX3CL1, and Galectin-3 rat ELISA kits, Sigma) using apical or basal supernatants from individual Transwell® inserts from the same experiment than the multiplex array.
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bioRxiv - Molecular Biology 2024Quote: ... genomic DNA was extracted from cultures that finished sexual development using GeneElute – Mammalian Genomic DNA Miniprep Kit (Merck-Sigma, Germany). PCRs were done on specific genomic regions flanking an IES (Table S6 ...
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bioRxiv - Neuroscience 2024Quote: Serum insulin and leptin were measured by an enzyme-linked immunosorbent assay (ELISA) kit (#EZRMI-13K and #EZML-82K; Millipore). Serum triglyceride levels and total cholesterol were measured using a commercial colorimetric assay kit (LaborLab® ...
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bioRxiv - Neuroscience 2024Quote: ... Free and total cholesterol levels were determined in sciatic nerves methanolic extracts by cholesterol quantitation kit (MAK043–1KT–Sigma Aldrich) following manufacturer’s instructions.
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bioRxiv - Genomics 2024Quote: ... and plasmid DNA was purified from the resulting cell pellet using a GenElute HP Plasmid Maxiprep Kit (Sigma-Aldrich #NA0310) following the manufacturer’s recommended protocol with elution in 3 mL H2O ...
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bioRxiv - Developmental Biology 2024Quote: Total ATP content in a pool of 5 oocytes was determined using the Adenosine 5′-triphosphate (ATP) bioluminescent somatic cell assay kit (FLASC, Sigma) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Infected erythrocytes were labeled with a membrane dye using the PKH26R Red Fluorescent Cell Linker Midi Kit (Sigma #MIDI26-1KT) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Lipolysis was assessed from the release of FFA and glycerol in the secretion samples using non-esterified fatty acids measurement kit (FUJIFILM Wako Chemicals Europe GmbH) and free glycerol reagents (G7793 and F6428, Sigma) respectively and normalised to total protein contents.
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bioRxiv - Immunology 2023Quote: ... EasySep™ Human NK Cell Isolation Kit) and were frozen down in CS10 CryoStor cell cryopreservation media media (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2022Quote: RNA used for ONT sequencing was poly(A+) selected in two consecutive rounds using oligo(dT) beads (GenElute mRNA Miniprep Kit, Sigma MRN10 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were fixed 72 h after transfection in -20°C methanol and subjected to PLA using the Duolink In Situ Red Starter Kit Mouse/Rabbit (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... After 24-hours BrdU was added to the media and 16h later cell proliferation was evaluated using the BrdU Cell Proliferation ELISA kit (EMD-Millipore). Colorimetric analysis was done with single channel 450nm intensity ...
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bioRxiv - Immunology 2023Quote: ... 3×106 cells were activated for 3h and lysates were obtained as described in the Technical Bulletin accompanying the Aconitase Assay Kit from Sigma. Aconitase activity in the lysates is determined per manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... was added to the reaction mixture along with 10 μL of (NH4)Fe(SO4)2/cysteine solution (Aconitase Assay Kit, Sigma). The mixture was incubated for 30 min at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... and tumor necrosis factor α (TNFα)) was performed using the Milliplex Mouse Cytokine Magnetic Kit (EMD Millipore, Cat # MCYTOMAG-70K) and Luminex MAGPIX instrument at the Vanderbilt Hormone Assay and Analytical Service Core ...
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bioRxiv - Cancer Biology 2023Quote: RIP assay was performed following the manufacturer’s instructions (EZ-Magna RIP RNA-binding Protein Immunoprecipitation Kit, Sigma-Aldrich, #17-701). Antibody against eIF4G was used for the part of immunoprecipitation ...
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bioRxiv - Genomics 2023Quote: To obtain sufficient nuclei to run the same suspension across both fixed kits we used EZ lysis buffer (Sigma NUC101), at the expense of greater debris ...
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bioRxiv - Cancer Biology 2023Quote: Proximity ligation assays (PLA) were performed using the Duolink® In Situ Red Kit Mouse/Rabbit (Sigma-Aldrich, Cat # DUO92101). For in vitro PLA ...
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bioRxiv - Cell Biology 2023Quote: ... histone H1.2 knockout heterozygous (Het) and homozygous (KO) mice are isolated using the Nuclei Isolation ‘Kit Nuclei EZ Prep’ (NUC101, Sigma). Then the nuclei are treated with 500 µM MNNG with fresh-made NAD+ (N0632 ...
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bioRxiv - Biochemistry 2023Quote: ... acetyl-CoA was quantified in a 96-well microplate format by a commercial fluorescence assay kit (MAK039, Sigma Aldrich, USA) according to the supplier’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... free phosphate release in solution was analyzed by malachite green based colorimetric assay using a Phosphate assay kit (Sigma-Aldrich) by following manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: The serum alanine aminotransferase (ALT) activity was analysed using a commercially available kit according to the manufacturer’s guidance (Sigma-Aldrich) and a Spark multimode microplate reader (Tecan ...
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bioRxiv - Immunology 2023Quote: ... the proteins of the parietal-cell cytosol and membrane/organelle fractions were extracted with the ProteoExtract Subcellular Proteome Extraction Kit (Millipore) to exclude the nucleic and cytoskeletal fractions ...
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bioRxiv - Plant Biology 2023Quote: ... and RNA was extracted from three to four replicates per plant and stage using the Spectrum Plant Total RNA kit (SIGMA). MagNA Lyser Green Beads (Roche ...
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bioRxiv - Physiology 2023Quote: Free fatty acid (FFA) content was quantified in mouse plasma samples using the Free Fatty Acid Quantitation Kit (Sigma, #MAK0044) in line with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... were allowed for growth in 6-cm cell culture plates for 48 h and subjected to isolation of the cytoplasmic and nuclear fractions by using a relevant separation kit (N-3408-200ML, Sigma). During this procedure ...
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bioRxiv - Immunology 2023Quote: ... and aspartate aminotransferase (AST) concentrations were measured using an AST and ALT Activity Assay Kit (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Physiology 2023Quote: ... GLP-1 content in the supernatant of T0 and T20 samples was assessed using a Glucagon-Like Peptide-1 (GLP-1) Total ELISA KIT (96-Well Plate Cat. # EZGLP1T-36K, EZGLP1T-36BK, Millipore).
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bioRxiv - Plant Biology 2023Quote: ... bacteria were harvested by centrifugation and pellets were resuspended in 5 ml of binding buffer (His-bind buffer kit (Millipore), proteins were extracted using a Constant cell disrupter system (Constant Systems ...
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bioRxiv - Microbiology 2023Quote: ... the cultures were quantified by isolating the genomic DNA from 1ml of inoculate using GenElute Bacterial Genomic DNA kit (Sigma) and measuring the genomic equivalents (GE ...
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bioRxiv - Immunology 2023Quote: ... The protein concentration in the supernatant was measured by the BCA protein assay kit (Sigma-Aldrich, cat. No. BCA1-1KT).
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bioRxiv - Neuroscience 2023Quote: ... neurons were fixed at 4 days in vitro (DIV4) with 4% PFA and the Duolink PLA kit was employed as previously described 7 following the manufacturer’s (Sigma-Aldrich) protocol 45.
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bioRxiv - Biochemistry 2023Quote: ... A standard cell-free reaction (2-3 ml of RM) was carried out in 50 ml tubes using a Pur-A-Lyzer Maxi dialysis kit (#PURX35050, Sigma). The TrkBtm was expressed as a precipitate ...
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bioRxiv - Cell Biology 2023Quote: ER was purified according to the protocol optimised by combining the traditional microsome-based density gradient isolation method (Endoplasmic Reticulum Isolation Kit developed by Sigma) with the cell surface biotinylation reaction method (developed and optimised by Pierce) ...
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bioRxiv - Biochemistry 2023Quote: ... the samples were further treated in a 96-well plate following the “Coenzyme A Assay Kit” protocol from Sigma-Aldrich. The absorbance of the colorimetric product (570 nm ...
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bioRxiv - Bioengineering 2023Quote: ... The phosphate concentration was then measured according to the manufacturer’s instructions (Malachite Green Phosphate Assay Kit, Sigma-Aldrich, The Netherlands). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... NPCs were differentiated to astrocytes using the Stem Cell Technologies STEMdiff astrocyte differentiation and maturation kits during which they were split weekly with Accutase (Millipore).
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bioRxiv - Cell Biology 2023Quote: ... Tissue was then blocked as per the manufacturers conditions before proceeding with the assay using the Duolink In situ proximity ligation assay PLA kit (Sigma). Antibodies used for PLA ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μM of carbonylcyanide-4-(trifluoromethoxy)-phenylhydrazone (FCCP) (Seahorse XF Cell Energy Phenotype Test Kit from Agilent or Sigma Aldrich), and 1 μM of antimycin A and rotenone (Sigma Aldrich) ...