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Citations for Millipore Sigma :
6701 - 6750 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: Snap frozen lungs were homogenized and nuclear extracts were obtained using the CelLyticTM NuCLEARTM Extraction Kit (Millipore-Sigma). Protein concentrations were determined by Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... and fruits of three replicate were isolated by using RaFlex Total RNA isolation Kit (Merck Millipore, Massachusetts, USA) by following the standard protocol defined by the manufacturer ...
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bioRxiv - Neuroscience 2022Quote: ... or MG132 using a MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-based in vitro toxicology kit (TOX1-1KT, Sigma). 10 µl MTT solution was applied to cells at the end point of the experiment and incubated for 4 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Congo red staining was performed using a Congo red amyloid stain kit procedure (Sigma Aldrich, St Louis, MO). Pathological tau was evidenced with CP13 (1/50 ...
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bioRxiv - Physiology 2024Quote: ... Citrate synthase activity in C2C12 myotubes was measured with a Citrate Synthase Activity Assay Kit (MAK193, Sigma-Aldrich) and following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 1 µL of the supernatant was then mixed with 4 µL of glucose assay kit (Sigma GAHK20-1KT) and incubated for 1 h at 37°C ...
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bioRxiv - Neuroscience 2024Quote: Cell viability was measured using the cell counting kit-8 (CCK-8) (Sigma-Aldrich, St. Louis, MO, USA) as previously described 104 ...
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bioRxiv - Immunology 2024Quote: Plasma aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were assessed using kits from Sigma-Aldrich (St. Louis, MO), and IL-1β was assessed using a kit from R&D systems (Minneapolis ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were extracted from the residue debris pellets and quantified using a BCA kit (Sigma-Aldrich, Taufkirchen, Germany). Lipid profiling was performed using a Sciex ExionLC AD coupled to a Sciex ZenoTOF 7600 under control of Sciex OS 3.0 (Sciex ...
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bioRxiv - Molecular Biology 2024Quote: ... Blue Dextran from the Gel Filtration Molecular Weight Markers Kit for Molecular Weights 29,000–700,000 Da (Sigma Aldrich) was used as a void volume marker and the six proteins (Albumin ...
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bioRxiv - Molecular Biology 2024Quote: ... the cells were incubated with anti-mouse minus and anti-rabbit plus PLA probes (PLA kit from Sigma) at 37°C for 1 hour ...
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bioRxiv - Microbiology 2024Quote: ... α-Amylase activity assay was performed following the user manual of an Amylase Activity Assay Kit (Sigma-Aldrich). Total salivary esterase activity was conducted according to the method adapted from Pérez-Jiménez et al ...
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bioRxiv - Neuroscience 2024Quote: ... Upper layer was used for TG measurement using Serum Triglyceride Determination kit (TR0100, Sigma Aldrich, Saint Louis, USA). Serum leptin was measured with Mouse Leptin ELISA KIT #90030 ...
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bioRxiv - Neuroscience 2023Quote: ... 1 µL of each sample was then mixed with 4 µL of glucose assay kit (Sigma GAHK20-1KT) and incubated for 1 h at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... All constructs were prepared for transfection using either the GenElute HP Endotoxin-Free Plasmid Maxiprep Kit (Sigma-Aldrich) or the NucleoBond Xtra Midi EF kit (MACHEREY-NAGEL) ...
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bioRxiv - Cell Biology 2023Quote: ... All constructs were prepared for transfection using either the GenElute HP Endotoxin-Free Plasmid Maxiprep Kit (Sigma-Aldrich) or NucleoBond Xtra Midi EF kit (MACHEREY-NAGEL).
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bioRxiv - Cancer Biology 2024Quote: ... 5 µl of Alexa Fluor 488 click reaction cocktail (Click-iT Plus EdU Flow Cytometry Kit; Sigma C10633) was added for 30 minutes in the dark at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... we extracted total RNA with the GenElute™ Mammalian Total RNA Miniprep Kit (#RTN350-1KT, Sigma Aldrich, USA). For both RNA-Seq and WGBS ...
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bioRxiv - Developmental Biology 2024Quote: The concentration of TGs in embryos was measured using the Triglyceride Quantification Colorimetric Kit from Sigma (Cat. #MAK266). 50 stage5 embryos were homogenized in Eppendorf tubes on a Nonidet P40 Substitute (Sigma ...
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bioRxiv - Genomics 2024Quote: ... and full blooming flowers (i.e. petals were fully unfolded) using the Spectrum Plant Total RNA Kit (Sigma Aldrich) and generated 80.00 million PE 150-bp reads (Illumina NovaSeq ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Protein quantification for each sample was conducted by BCA Assay in accordance with kit instructions (Millipore Sigma 71285M).
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bioRxiv - Cell Biology 2024Quote: ... the invaded cells were fixed and stained with a Harleco Hemacolor staining kit (EMD Millipore, Oakville, ON, Canada). The non-invaded cells remaining at the top of the membrane were removed with a cotton swab ...
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bioRxiv - Cancer Biology 2024Quote: ... genomic DNA was extracted from cryopreserved patient tissue samples using GenElute Mammalian Genomic DNA Miniprep Kit (Sigma-Aldrich) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: The proximity ligation assay was performed using the Duolink In Situ Orange starter kit mouse/rabbit (Millipore-Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: The proximity ligation assay was performed using the Duolink In Situ Orange starter kit mouse/rabbit (Millipore-Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Total RNA was isolated from 1 million cells in triplicates using GenElute Mammalian Total RNA Miniprep Kit (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Proximity ligation assay (PLA) was performed using the Duolink In Situ Red Starter Kit Mouse/Rabbit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The results from capillary electrophoresis were subsequently benchmarked against a commercially available creatine assay kit (MAK079, Sigma-Aldrich), which measures creatine without phosphocreatine due to a lack of creatine phosphokinase ...
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bioRxiv - Cell Biology 2023Quote: ... and triglyceride from HTM cells were extracted and measured with a phospholipid quantification assay kit (#CS0001, Sigma-Aldrich), cholesterol quantitation kit (#MAK043 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and three stages of development of the floral head) using a Spectrum Plant Total RNA Kit (Sigma, USA) with on-column DNA digestion following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Quantification of carbohydrates in the dried hydrolysates was performed using the total carbohydrate assay kit from Sigma-Aldrich according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Enzyme-linked immunosorbent assay (ELISA) kits were used to measure the levels of mouse HGF (Sigma-Aldrich, RAB0214), mouse EGF (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... the protein concentrations were measured using a standard BCA assay with Pierce™ BCA Protein Assay Kit (Sigma). 30 μg of proteins was loaded into a pre-cast gel (Novex™ 16% Tricine Gels ...
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bioRxiv - Biochemistry 2023Quote: In vitro DHFR activity was analyzed by using a colorimetric assay kit according to manufacturer’s instructions (Sigma-Aldrich) to quantify DHFR-dependent NADPH turnover by monitoring absorbance at 340 nm ...
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bioRxiv - Microbiology 2023Quote: ... All pCC2Fos replicons were extracted using the GenEluteTM HP Plasmid Miniprep Kit (Sigma Aldrich, St. Louis, Missouri, USA) and NucleoBond® Xtra Midi Plus (MACHEREY-NAGEL ...
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bioRxiv - Microbiology 2022Quote: ... the presence of Fucα1-2Gal-R was detected with a TMB (3,3’,5,5’-tetramethylbenzidine) kit (Sigma-Aldrich, USA), and the signal intensities [the optical density at 450 nm (OD450)] were read with a Multiskan Sky plate reader (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... Samples extracted with Direct-zol RNA miniprep kit were disrupted by vortexing with glass beads (Sigma-Aldrich #Z250465) for 5 minutes in TRI reagent ...
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bioRxiv - Cancer Biology 2023Quote: The Duolink in situ PLA was performed using Duolink In Situ Red Starter Kit Mouse/Rabbit (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: Mouse High Sensitivity T Cell Magnetic Bead kits were purchased from EMD Millipore (MHSTCMAG-70KPMX, Burlington, MA, USA) and used to quantify 18 different mouse cytokines in accordance with the manufacturer’s recommendations (belonging to 4 main categories ...
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bioRxiv - Bioengineering 2023Quote: ... The conversion of pyruvate to lactate by lactate dehydrogenase was assessed with a pyruvate assay kit (Sigma, MAK332), and the color change of X-gal was observed in the channels and buffers for the β-galactosidase assay.
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bioRxiv - Plant Biology 2023Quote: ... The acetate content in the supernatant was quantified using the Acetate Colorimetric Assay Kit (Sigma, Cat No. MAK086) according to manufacture instructions.
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bioRxiv - Plant Biology 2023Quote: ... qPCR data shown in figures 3B and S3B was done using the Spectrum RNA extraction kit (Sigma-Aldrich), followed by DNase treatment using AMPD1 DNase I (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... ATP and ADP were extracted and quantified using the ATP/ADP ratio quantification kit (MAK-135, Sigma Aldrich) as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... proximity ligation assay (PLA) was performed in accordance with the Duolink kit (Sigma-Aldrich, Saint Louis, MO, USA). Briefly ...
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bioRxiv - Plant Biology 2023Quote: ... Tissue was grounded in liquid nitrogen and RNA was extracted with the Spectrum Plant Total RNA Kit (Sigma) including on-column DNase digestion (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... after 42°C heat shock transformation and extracted using the GenElute HP Plasmid Miniprep Kit (Sigma-Aldrich, Merck). To generate pARL-PfCen1-GFP ...
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bioRxiv - Systems Biology 2023Quote: ... Proximity ligation was performed with the Duolink In Situ Orange Starter Kit Mouse/Rabbit (Millipore Sigma, DUO92102-1KT) according to the manufacturer’s recommendation ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA was extracted from cells using the GenElute™ Mammalian Total RNA Miniprep Kit (Sigma-Aldrich, RTN350). cDNA was reversely transcribed from 1 µg of RNA using the iScript cDNA synthesis kit (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: The intracellular ROS levels in C6 cells were determined using the Fluorometric Intracellular ROS Kit (MAK142; Sigma, USA), which was performed in a 96-well plate format with detection at an excitation/emission wavelengths of 650/675 nm 29.
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bioRxiv - Plant Biology 2023Quote: ... The extract was used for estimation of oxalate with oxalate assay kit (Sigma-Aldrich, St Louis, MO, USA) following the manufacturer’s protocol.