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Citations for Millipore Sigma :
6701 - 6750 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 4.8 L of the pre-filtered seawater was again filtered through polycarbonate membrane filters (142-mm diameter, 0.2-μm pore size; Millipore) to isolate coastal prokaryotic cells ...
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bioRxiv - Microbiology 2024Quote: ... membranes were washed in TBS-T and incubated in anti-mouse secondary antibody (Sigma-Aldrich, 12-349, 1:3000) and anti-rabbit secondary antibody (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was blocked overnight at 4°C in 5% BSA (SRL) in 1X PBS containing 0.05% Tween20 (Sigma) (wash buffer) ...
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bioRxiv - Microbiology 2024Quote: ... coli in a final volume of 20 µl LB and spotted on nitrocellulose filter membranes with a 0.22 µm pore size (Millipore) located on LB agar plates supplemented with 1.2 mM DAP ...
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bioRxiv - Genomics 2024Quote: ... or microporous membrane inserts (cellQART 931 10 12, pore size 1µm) coated with Concanavalin A (Sigma-Aldrich C-2010) and Laminin (VWR 47743-734) ...
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bioRxiv - Neuroscience 2024Quote: ... The resolved proteins were transferred from the gel to Immobilon®-P PVDF membranes (Millipore Sigma, Cat# IPVH00010, USA) in 10x Tris/Glycine buffer (BioRad ...
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bioRxiv - Cancer Biology 2024Quote: ... proteins were transferred to a nitrocellulose blotting membrane (Amersham 0.45 µm, 10600016) and blocked in 5% milk in PBS/0.1% Tween (Sigma, P1379) followed by primary antibody incubation overnight in a cold room ...
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bioRxiv - Biochemistry 2024Quote: ... and the protein was filtered through polyvinylidene fluoride ultra-free membrane filter with a 0.22 μm pore size (Millipore). The concentrated and filtered sample was then finally passed through a HiLoad 10/300 Superdex 75 column (GE Healthcare ...
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bioRxiv - Microbiology 2024Quote: ... a 1 μl bait inoculum was spotted on an approximately 5 mm x 5 mm sterile 0.22 μm pore size nitrocellulose membrane (Millipore) positioned at the usual bait inoculation location ...
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bioRxiv - Microbiology 2024Quote: ... A 5 mL of the fixed co-culture was filtered on a polycarbonate membrane filter with 0.1 μm pores (VCTP, 25mm, Merck Millipore). Cells trapped on the filter were washed by passing through 500 μL of pure water five times ...
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bioRxiv - Neuroscience 2024Quote: ... Protein lysates were resolved on 4-12 % precast Bis-Tris gels (Thermo) or poured 7% Bis-Tris gels and transferred to 0.2 μm PVDF membranes (Millipore). Membranes were blocked with 5% non-fat milk in TBST and then probed overnight with primary antibodies in 5% non-fat milk in TBST at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... 150 to 2,000 mL (Table S1.1) of pre-filtered water were filtered onto 0.22-µm membranes (PES, Millipore Express, Germany). Filters were flash-frozen in liquid nitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... sonicated for 5 min and purified for 5 min at 5000 rcf in centrifugal filter units (PVDF membrane with 0.22 μm pore size; Millipore). A slowly tapering glass pipette was pulled from a borosilicate glass capillary (borosilicate glass capillaries ...
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bioRxiv - Neuroscience 2024Quote: ... Two to three slices with well-defined hippocampal neuronal layers were placed onto each semi-porous membrane insert (0.4 um, Millipore) inside a 35 mm culture dish ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cell lysates were transferred to a 0.45 µm 96-well filter-plate (Durapore, low protein-binding PVDF membrane, Merck Millipore), centrifuged for 5 min at 500 xg and 4°C to remove aggregates and collected in a 96 well polypropylene plate ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 60 µL were carefully taken off from each ultra-centrifugation tube and transferred into a 0.2 µm 96-well filter-plate (Durapore, low protein-binding PVDF membrane, Merck Millipore), centrifuged for 5 min at 500 xg and 4°C and the flow through was collected in a 96 well polypropylene plate ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cell supernatants from all samples were carefully collected and 230 µL supernatant was transferred into a 0.45 µm 96-well filter-plate (Durapore, low protein-binding PVDF membrane, Merck Millipore) to remove detached cells and cell debris ...
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bioRxiv - Microbiology 2024Quote: ... Blocked membrane was incubated overnight at 4 °C with primary antibody anti-FLAG (F3165, Sigma-Aldrich, 1:2000 dilution), washed 3 times in TBS with 1% Tween 20 (TBS-T) ...
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bioRxiv - Molecular Biology 2024Quote: Equal amount of protein (50-100μg) or immunoprecipitation samples were run on SDS-PAGE and transferred to PVDF membrane (Millipore). The membranes were then blocked in 5% skimmed milk at room temperature for 1 hour on the rocker ...
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bioRxiv - Physiology 2024Quote: ... Equal loading and protein transfer to the membrane were confirmed by Ponceau S staining (Sigma-Aldrich, St. Louis, MO). The chemiluminescence images were digitally captured using G:BOX mini imaging system (Syngene ...
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bioRxiv - Neuroscience 2024Quote: ... membranes were blocked for 1 hour in 0.5% BSA/nonfat dried milk in 1X TBST (J77500-K2, Sigma-Aldrich) and incubated with Monoclonal Anti-U2AF65 antibody (U4758 ...
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bioRxiv - Cancer Biology 2024Quote: ... 30 µg of total protein was loaded onto an SDS-PAGE gel and transferred to PVDF membranes (Millipore, USA). After blocking with 5% BSA ...
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bioRxiv - Biochemistry 2024Quote: ... An equal amount of protein was analyzed using SDS-PAGE and transferred to polyvinylidene fluoride PVDF membranes (0.22 μm pore size, Millipore). The membranes were blocked with Odyssey Blocking Buffer (LI-COR ...
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bioRxiv - Genetics 2024Quote: ... membranes were washed thrice in PBST for 5 min each and the incubated with anti-mouse (Sigma-Aldrich, F1804) or anti-rabbit (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were resolved by glycine-Tris SDS–PAGE followed by transfer onto a 0.2 μm polyvinylidene fluoride membrane (Immobilon, Millipore). Membranes were blocked with 3% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2024Quote: ... Whole cell lysates were loaded onto 12% SDS-PAGE (FD346; FUDE) followed by transfer to PVDF membranes (IPVH00010; Millipore). After protein transfer ...
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bioRxiv - Cell Biology 2024Quote: Protein samples were separated by denaturing electrophoresis on 6%-10% SDS‒PAGE gels (Epizyme) and transferred to 0.45 μm PVDF membranes (Millipore) via a wet transfer system (Tanon) ...
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bioRxiv - Cell Biology 2024Quote: ... and run at 120 V for 75 mins before transfer to PVDF transfer membrane (0.45 µm pore size, Millipore) for 240 mins at 80 V at 4ºC ...
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bioRxiv - Cancer Biology 2024Quote: ... CRISPR/Cas9-edited CD34+ cells were stained with CellVue Claret Far Red Membrane Label (Sigma-Aldrich, Cat#MINCLARET-1KT) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked in 5% BSA powder in TBST (25 mM Tris, 150 mM NaCl, 0.2% Tween-20 (Sigma), pH 7.4 adjusted with HCl ...
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bioRxiv - Cell Biology 2024Quote: ... The membranes were washed with TBS-Tween (0.01% v/v) prior to detection using enhanced chemiluminescent (ECL) reagent (Millipore) and imaged using a ChemiDoc Imaging System (BioRad) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 4× cycles of ultrafiltration in Amicon Ultra-0.5 Centrifugal Filter Units with Ultracel-10 regenerated cellulose membranes (10 kDa nominal molecular weight limit, NWML) (Millipore) using mQ water as exchange buffer were performed following the manufacturer’s recommendations ...
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bioRxiv - Evolutionary Biology 2024Quote: ... an additional cleaning step was introduced before the ultrafiltration to remove high molecular weight co-extracted proteins using Amicon Ultra-0.5 Centrifugal Filter Units with Ultracel-30 regenerated cellulose membranes (30 kDa, NWML) (Millipore).
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bioRxiv - Immunology 2021Quote: ... Several samples were also screened with MILLIPLEX MAP Human Cytokine/Chemokine 41-Plex cytokine assay (Millipore). Cytokines in mouse BALF were analyzed with the Mouse CXCL1/KC DuoSet ELISA (#DY453 ...
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bioRxiv - Physiology 2021Quote: ... The 10 ng/ml insulin standard (8013-K) and assay buffer (AB-PHK) were from Millipore.
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bioRxiv - Evolutionary Biology 2021Quote: Assay standards for Fe(II) ranging from 150 µM to 2.34 µM FeSO4•7H2O (Sigma-Aldrich) and Fe(III ...
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bioRxiv - Developmental Biology 2020Quote: ... the total GAG content of cell pellets was quantified by the dimethylmethylene blue assay (Sigma-Aldrich) and normalized with the DNA content determined separately by the PicoGreen assay (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Cytokine/chemokine measurements in sera were performed using U-PLEX Adipokine assays (Meso Scale Discovery, Millipore).
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bioRxiv - Cancer Biology 2020Quote: The QEM Endothelial cell invasion assay was carried on in accordance with manufacture’s recommendations (ECM210, Millipore). Briefly ...
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bioRxiv - Genomics 2021Quote: ... Prior to protein quantification milk was diluted 1:2 in assay buffer (Merck-Millipore, Burlington, USA). Concentrations for 15 cytokines (IL-1α ...
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bioRxiv - Cell Biology 2021Quote: The Caspase-3 colorimetric assay was also conducted according to manufacturer instructions (Sigma-Aldrich, MO, USA). 3T3-L1 cells were treated with Bhasma for 0 ...
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bioRxiv - Cell Biology 2021Quote: ... cell cycle and apoptosis assays were carried out using a Muse Cell Analyzer (Millipore, Sigma-Aldrich) following the manufacturer’s recommendations.
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bioRxiv - Cell Biology 2021Quote: ... cell cycle and apoptosis assays were carried out using a Muse Cell Analyzer (Millipore, Sigma-Aldrich) following the manufacturer’s recommendations.
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bioRxiv - Bioengineering 2021Quote: ... The viability of cells was assessed using live/dead assay (calcein-AM/propidium iodide, Sigma Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... Quantitative calcium analyses of explanted BP discs were conducted using a colorimetric calcium assay (Sigma-Aldrich), per the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... The assay was performed in RPMI-1640 buffer without phenol red and carbonate buffer (Sigma-Aldrich) containing 25 mM glucose ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and a colorimetric LDH release assay performed as per manufacturer’s instructions (Sigma-Aldrich; cat no: MAK066). Absorbance (450 nm ...
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bioRxiv - Biochemistry 2021Quote: ... the concentrated urinary proteomes were quantified via a 96 well plate Bradford protein assay (Sigma-Aldrich) using and Bovine Serum Albumin to generate the calibration curve.
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations were estimated by the bicinchoninic acid (BCA) protein assay method (Sigma-Aldrich, Merck, USA). Proteins (50 μg ...
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bioRxiv - Cell Biology 2020Quote: ... Final drug concentrations used in mammalian MDC assays were 25 mg/ml cycloheximide (Sigma-Aldrich; C1988), 250 nM Torin1 (R&D Systems ...