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Citations for Millipore Sigma :
6651 - 6700 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... membranes were incubated overnight at 4°C in the following primary antibodies: Fas (EMD Millipore 05-351, 1:500), Phospho-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were resolved on 10% SDS-PAGE and transferred to PVDF membrane (Immobilon-FL, Millipore Corp., cat. no. IPFL00010) using a ThermoFisher Powerblotter (cat ...
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bioRxiv - Immunology 2023Quote: ... Proteins were separated by SDS-polyacrylamide gel electrophoresis and transferred onto a nitrocellulose membrane (Amersham Protran, GE10600001, Sigma Aldrich) by the wet transfer method ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were washed three times for 5 min with Tris-buffered saline with 0.05% Tween 20 (Sigma-Aldrich) and incubated with the secondary (Santa Cruz Biotech ...
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bioRxiv - Cell Biology 2023Quote: ... The cell extracts and SN were resolved in 15% SDS-PAGE gels and transferred to PVDF membranes (#ISEQ00010, Millipore) at 100V during 3 h ...
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bioRxiv - Immunology 2023Quote: ... membrane at the bottom (Multiscreen HTS 0.45 μm Hydrophobic High Protein Binding Membrane 96-well Filtration plate (Product No. MSIPS4510; Millipore-Sigma) (65 ...
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bioRxiv - Neuroscience 2023Quote: ... the membrane was incubated overnight at +4°C with the following primary antibodies: anti-TGFBR2 (1:1000, SAB5700739, Sigma), anti-TGFB1 (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... The diluted reaction mixture was then purified by UF/DF using 10K MWCO PES membrane (Millipore Pellicon 2 Mini) against PBS buffer (30X ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were then transferred to a nitrocellulose membrane and blocked with PBS + 0.1% Tween 20 + 5% Milk powder (Sigma) and incubated overnight with primary antibodies at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was probed with an α-FLAG antibody (Sigma; 1:1,000 in 1x TBS-T containing 3% BSA) at 4 °C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... The upper layer was removed and filtered with a 0.45 μm pore size Durapore membrane Ultrafree-HV microcentrifuge filtration unit (Millipore). Samples were mixed with an equal volume of 0.3% NaPTA and 2% sarkosyl and incubated at 37 °C for 10 min before centrifugation for 90 min at 16 ...
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bioRxiv - Bioengineering 2023Quote: ... The membranes were then washed three times with 1x PBS supplemented with 0.1% Tween 20 (Sigma Aldrich, P1379; PBST) for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... Infected CAD5 cells were seeded in ELISPOT plates (multiscreen 96-well filter plates with high protein-binding Immobilon-P membrane, 0.45 µm, Millipore) activated with 70% ethanol ...
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bioRxiv - Cancer Biology 2023Quote: ... USA) The membrane was stripped and re-stained using an anti-β-actin AC-15 antibody (Sigma Aldrich, USA). Western blot bands were quantified relative to the β-actin loading control using Fiji (ImageJ).[101]
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bioRxiv - Cancer Biology 2023Quote: ... PVDF membrane was blocked by 30 min incubation in TBS-T (TBS 10x: 200mM Tris (Sigma, #1185-53-1), 1.37M NaCl (Sigma ...
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bioRxiv - Immunology 2023Quote: ... Proteins were subsequently transferred onto nitrocellulose membranes and immunoblot analysis was performed with following antibodies: HA (Sigma-Aldrich, H6908), FLAG (M2 ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were incubated overnight with primary antibodies diluted in 5% BSA in TBS (Fisher) with 0.1 % Tween20 (Sigma-Aldrich). Membranes were incubated with secondary antibodies (anti-mouse or anti-rabbit ...
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bioRxiv - Microbiology 2023Quote: ... A volume of 2.5 μL (OD600 ∼0.5) was used to seed sterile 13-mm polycarbonate membrane (WHA10417401, Sigma-Aldrich) on TSA plates and incubated at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... equal amounts of protein per sample were subjected to SDS–PAGE and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore). For immunoprecipitation of FLAG-tagged proteins ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked in TBS+0.1% tween and milk or 5% bovine serum albumin (Sigma-Aldrich, cat# A3912-50G) in TBS+0.1% tween (phospho-protein samples ...
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bioRxiv - Neuroscience 2023Quote: ... The input sample and washed beads sample were subjected to 8% SDS-PAGE gels and transferred to 0.45 μm PVDF membranes (Millipore), the membrane was blocked by 5% Nonfat dry milk (BIO-RAD ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was incubated for 2 h at room temperature with a monoclonal anti-FLAG antibody (Sigma Aldrich, F1804) diluted 1:1,000 in blocking buffer ...
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bioRxiv - Microbiology 2023Quote: ... and separated on SDS-PAGE gels (10–12.5% polyacrylamide) and electro-transferred to the polyvinylidene difluoride (PVDF) membrane (Millipore). The membranes were then blocked in 5% nonfat dry milk (NFDM ...
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bioRxiv - Microbiology 2023Quote: ... A Hybond N+ membrane (GE lifesciences, 45000854) and 6 sheets of 3 mm Whatman cellulose paper (Sigma Aldrich, WHA3030861) were pre- soaked for 5 minutes in room temperature 0.5X TBE ...
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bioRxiv - Microbiology 2023Quote: ... The phosphorylated histidine was detected by Western blotting by then incubating the membrane for 60 min with 1:1.000 anti-N1-phosphohistidine antibody (Merck Millipore) in TBS-T followed by a washing step for 30 min with TBS-T and a final 60 min incubation with with 1:10.000 horseradish peroxide-conjugated anti-rabbit IgG (Promega ...
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bioRxiv - Microbiology 2023Quote: ... Antibodies bound to the cell mono-layers were detected by the addition of 3,3’,5,5’-tetramethylbenzidine liquid substrate system for membranes (Sigma-Aldrich). The cell culture infectious dose 50% (CCID50 ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein samples were run on EZ Protein any KD PAGE (LIFE-iLAB) and then transferred to 0.22um PVDF membranes (Millipore). Membranes were blocked using 5% non-fat milk in PBS-Tween20 0.1% for 1 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... SDS samples were subjected to SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to Immobilon-P membranes (Millipore, IPVH00010). The membranes were blocked with 5% skim milk (Megmilk Snow Brand ...
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bioRxiv - Cancer Biology 2023Quote: ... 30–50 μg of protein was fractionated by 10% SDS–PAGE and blotted onto an Immobilon PVDF membrane (Millipore). Non-specific binding sites were blocked by incubation with 5% BSA in Tris-buffered saline (TBS ...
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bioRxiv - Microbiology 2023Quote: ... Membrane attack complex (MAC) binding was assessed by staining with rabbit anti-human C5b-C9 antibody (Catalog #204903, Sigma) at 1:1000 dilution at room temperature for 1 hour followed by washes x 3 then staining with goat anti-rabbit HRP-conjugated antibody (Catalog #204903 ...
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bioRxiv - Biochemistry 2023Quote: We measured TEER in of RPE plated on transwell filter membranes using an Epithelial Volt- Ohm Meter (Millipore, #MERS00002) and ERS Probes (Millipore ...
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bioRxiv - Molecular Biology 2023Quote: ... samples were centrifuged at 14,000 rpm at room temperature for 5 minutes or filtered in multiscreenHTS-HV 0.45 µm 96 well filter plates with PVDF membranes (Merck Millipore) to remove cell debris and nucleic acid aggregates ...
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bioRxiv - Neuroscience 2024Quote: ... The resolved proteins were transferred from the gel to Immobilon®-P PVDF membranes (Millipore Sigma, Cat# IPVH00010, USA) in 10x Tris/Glycine buffer (BioRad ...
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bioRxiv - Cancer Biology 2024Quote: ... proteins were transferred to a nitrocellulose blotting membrane (Amersham 0.45 µm, 10600016) and blocked in 5% milk in PBS/0.1% Tween (Sigma, P1379) followed by primary antibody incubation overnight in a cold room ...
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bioRxiv - Bioengineering 2024Quote: ... Supernatants were then centrifuged to remove cell debris and filtered through a 0.45 µm PVDF membrane (Millipore Sigma, SE1M003M00) prior to concentration using a 100 kDa Amicon centrifugal filter concentrator (Millipore Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... and the protein was filtered through polyvinylidene fluoride ultra-free membrane filter with a 0.22 μm pore size (Millipore). The concentrated and filtered sample was then finally passed through a HiLoad 10/300 Superdex 75 column (GE Healthcare ...
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bioRxiv - Microbiology 2024Quote: ... a 1 μl bait inoculum was spotted on an approximately 5 mm x 5 mm sterile 0.22 μm pore size nitrocellulose membrane (Millipore) positioned at the usual bait inoculation location ...
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bioRxiv - Microbiology 2024Quote: ... A 5 mL of the fixed co-culture was filtered on a polycarbonate membrane filter with 0.1 μm pores (VCTP, 25mm, Merck Millipore). Cells trapped on the filter were washed by passing through 500 μL of pure water five times ...
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bioRxiv - Microbiology 2024Quote: ... 150 to 2,000 mL (Table S1.1) of pre-filtered water were filtered onto 0.22-µm membranes (PES, Millipore Express, Germany). Filters were flash-frozen in liquid nitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... Protein lysates were resolved on 4-12 % precast Bis-Tris gels (Thermo) or poured 7% Bis-Tris gels and transferred to 0.2 μm PVDF membranes (Millipore). Membranes were blocked with 5% non-fat milk in TBST and then probed overnight with primary antibodies in 5% non-fat milk in TBST at 4°C ...
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bioRxiv - Physiology 2024Quote: ... to detect newly dead cells from the damage to the integrity of the cell membrane and DAPI (Sigma-Aldrich) to label cell nuclei ...
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bioRxiv - Plant Biology 2024Quote: ... The proteins were separated by 7.5% SDS-PAGE and transferred to an Immobilon-P membrane (Millipore, Billerica, MA, USA), as previously described (Towbin et al. ...
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bioRxiv - Cell Biology 2024Quote: ... To induce mitochondrial membrane depolarization MEF and HeLa cells were treated with 25µM or 10µM CCCP (Sigma-Aldrich #C2759), respectively ...
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bioRxiv - Cell Biology 2024Quote: ... Yeast protein extracts were separated on protein gels containing 10% polyacrylamide and transferred to an Immobilon-P membrane (Millipore). The membranes were probed with anti-Myc antibodies (9E10 ...
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bioRxiv - Microbiology 2024Quote: ... which was then vacuum filtered and dialyzed for 72 hours in water with a cellulose dialysis tubing membrane (Sigma). The colloidal chitin was then homogenized using a Bosch SilentMixx Pro blender ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was blocked using 5% skimmed milk (American Bioanalytical) in TBS with 0.1% Tween 20 (TBST; Sigma-Aldrich) and incubated in TBST overnight at 4°C with the following primary antibodies diluted 1:2000 ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were subjected to SDS-PAGE and transferred onto a polyvinylidene difluoride membrane (IPVH00010; Merck Millipore, Burlington, MA, USA). Antibodies against SPNS1 (HPA041995 ...
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bioRxiv - Immunology 2024Quote: ... Cells were seeded at 2.5×105 cells per well in at least duplicates in mixed cellular ester membrane plates (Millipore) which were previously (1-7 days ...
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bioRxiv - Cancer Biology 2024Quote: ... Supernatant containing lentivirus was collected 48 and 72 hr post-transfection and filtered through a 0.45-μm PVDF membrane filter (Millipore). To infect cells ...
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bioRxiv - Microbiology 2024Quote: ... The saturated donor and recipient cultures (100 µl each) were combined in 10 ml BHI and concentrated onto a 0.45-µm membrane filter disc (Millipore-Sigma) using vacuum filtration ...