Labshake search
Citations for Millipore Sigma :
6601 - 6650 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2024Quote: ... the membranes were incubated with goat anti-rabbit AP-conjugated antibodies (1:5000 in PBS, Sigma-Aldrich, Missouri, USA), followed by four washes with PBS-T ...
-
bioRxiv - Developmental Biology 2024Quote: ... Proteins were separated using sodium dodecyl-sulfate polyacrylamide gel electrophoresis and electrophoretically blotted onto microporous polyvinylidene fluoride membranes (Millipore) under a constant current ...
-
bioRxiv - Cancer Biology 2024Quote: ... Next the membrane was blocked with PBS-milk 5% and incubated with primary antibodies s9.6 1:500 (Millipore, MABE1095) and dsDNA 1:3000 (Abcam ...
-
bioRxiv - Microbiology 2023Quote: ... the membrane was incubated with anti-FLAG polyclonal primary antibody produced in rabbit (Sigma-Aldrich Cat# F7425, RRID: AB_439687) at 4° C overnight Then the membrane was washed 3 times in PBS-T (0.5% tween 20 in 1 x PBS) ...
-
bioRxiv - Microbiology 2024Quote: ... Proteins were resolved via SDS-PAGE in 7.5% polyacrylamide gels and transferred to 0.45 μm-pore PVDF membranes (Millipore, IPVH00010) using a semi-dry transfer method ...
-
bioRxiv - Molecular Biology 2024Quote: ... and the samples were separated on SDS‒PAGE gels and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, Cat# IPVH00010). The membranes were blocked with 5% w/v skim milk and incubated with primary and secondary antibodies before being washed with TBS-T buffer (20 mM Tris-HCl (pH 7.5) ...
-
bioRxiv - Microbiology 2024Quote: ... The supernatant was discarded and the pellet was washed with sterile PBS and filtered through a sterile 0.2 μm-pore polyvinylidene difluoride (PVDF) membrane (Millex GV; Millipore). Ultracentrifugation was repeated twice ...
-
bioRxiv - Molecular Biology 2024Quote: ... The membranes probed with the appropriate primary antibodies were incubated with secondary antibodies linked to horseradish peroxidase (HRP) (Sigma). Blots were revealed using Clarity Max Western ECL Substrate (Bio-Rad ...
-
bioRxiv - Neuroscience 2023Quote: ... The slices were incubated in this solution for 30 minutes and then placed on a Millicell-CM membrane (Millipore) in culture medium (50% Minimum Essential Medium ...
-
bioRxiv - Microbiology 2024Quote: ... 4 μl of the reaction were taken and deposited on a nitrocellulose filter (MF-Millipore 0.025 μm MCE Membrane) right after 2x 6 ml of reaction buffer was used to wash the filter under vacuum ...
-
bioRxiv - Cell Biology 2023Quote: ... proteins were separated on 6-10% SDS-PAGE gels and transferred to methanol pre-activated PVDF membranes (Millipore, #IPVH00010). For MYCBP2 blots ...
-
bioRxiv - Bioengineering 2024Quote: ... Samples were filtered through 0.33 mm diameter sterile syringe filters with a 0.22 µm hydrophilic polyvinylidene difluoride (PVDF) membrane (Sigma-Aldrich) and diluted to a final concentration of 25 µM for protein samples > 35 kDa ...
-
bioRxiv - Microbiology 2023Quote: ... the 96-well plate containing the cell-phage mixtures was mounted on top of a sterile 96-well MultiScreenHTS GV Filter Plate with sterile 0.22 µm membrane (Millipore) and centrifuged at 4000 x g to remove cells and adsorbed phages ...
-
bioRxiv - Genomics 2024Quote: ... The fragmented chromatin materials were filtered with Amicon Ultra-0.5 Centrifugal Filter with Ultracel-100 regenerated cellulose membrane (Millipore) to remove the remaining SDS and be concentrated ...
-
bioRxiv - Immunology 2024Quote: ... The homogenate was mixed 50 times using two syringes connected via a 25G needle and passed 5 times through a polyethersulfone membrane filter with pore size 0.22 μm (Millipore) and the filter-sterilized adjuvant was stored at 4°C ...
-
bioRxiv - Immunology 2024Quote: ... Cell supernatant was then concentrated by Tangential Flow Filtration with a Pellicon 3 Ultracel 10 kDa membrane (Merck Millipore) and loaded onto a 10 mL CaptureSelect™ C-tagXL affinity column that had been equilibrated in Tris-buffered saline (TBS ...
-
bioRxiv - Biochemistry 2024Quote: Concentrations of biogenic and lithogenic silica (bSi and lSi) were determined by filtering subsamples onto 0.6-μm pore size polycarbonate membrane filters (47 mm PC, Isopore, Millipore), which were frozen at sea in cryovials at −20°C ...
-
bioRxiv - Bioengineering 2024Quote: ... The hemolymph mixture was subsequently centrifuged at 10,000 x g for 10 min to remove hemolymph debris and filtered through a 0.45 µm PVDF membrane filter (Millipore). The filtered fraction was centrifuged at 45,000× g for 30 min.
-
bioRxiv - Cell Biology 2024Quote: ... This was conducted in the presence of 5 mM membrane-impermeable maleimide functionalized polyethylene glycol (mPEG, Sigma-Aldrich, 63187). This compound attaches a polyethylene glycol polymer chain of approximately 5 kDa to free thiol groups of proteins ...
-
bioRxiv - Cell Biology 2024Quote: ... Whole cell lysates were resolved by SDS-PAGE and then transferred to 0.2 m pore PVDF membrane (Immobilon, Millipore). The membrane was blocked with blot wash buffer containing 3% BSA ...
-
bioRxiv - Microbiology 2023Quote: ... 1.2 L of the pre-filtered seawater was filtered through polycarbonate membrane filters (142 mm diameter, 0.2 μm pore size; Millipore) to obtain prokaryotic cells and reduce the floating viral particles ...
-
bioRxiv - Cell Biology 2023Quote: ... Membranes were probed with the antibody of interest and then visualized by ECL (0.225 mM p-coumaric acid; Sigma), 1.25mM 3-aminophthalhydrazide (Luminol ...
-
bioRxiv - Neuroscience 2023Quote: ... The supernatants were filtered over Millipore Ultrafree-MC Centrifugal filters with a 0.22 μm pore size hydrophilic PVDF membrane (Millipore) and analyzed by HPLC system ...
-
bioRxiv - Cell Biology 2023Quote: ... All samples were then filtered in a 47 mm polytetrafluoroethylene polymer (PTFE) membrane (0.45 μm pores, Merck Millipore, USA) and rinsed three times with filtered ultra-pure water ...
-
bioRxiv - Microbiology 2023Quote: ... Protein samples were analyzed on an SDS–polyacrylamide (37.5:1) gel electrophoresis and blotted on 0.45 µm pore PolyVinyliDenFluoride (PVDF) membranes (Immobilon-P from Sigma Aldrich). Membranes were blocked 2h with 1X Tris-buffered saline (TBS ...
-
bioRxiv - Molecular Biology 2022Quote: ... Virus-containing medium was centrifuged at 400xg for 5 min to pellet cell debris before filtering through a 0.45μm Durapore PVDF membrane Steriflip (Millipore SE1M003M00). 4X Lenti-X Concentrator (TaKaRa 631232 ...
-
bioRxiv - Neuroscience 2023Quote: ... were separated by 10% Bis-Tris gels and semidry transferred to polyvinylidene fluoride (PVDF) membranes (0.45 μm pore size, Millipore). After being blocked by incubation with TBST containing 5% nonfat milk ...
-
bioRxiv - Cell Biology 2022Quote: ... SDS-PAGE was performed on pre-cast Tris-Acetate gels followed by transfer to PVDF transfer membrane (Merck Millipore). Proteins were detected using primary antibodies as described followed by incubation with secondary antibodies coupled with HRP (Invitrogen) ...
-
bioRxiv - Microbiology 2023Quote: ... and equal amounts of cell extracts were separated by SDS-12 % PAGE and transferred onto a nitrocellulose membrane (Sigma). Samples were then analyzed by western blot using the desired antibodies ...
-
bioRxiv - Neuroscience 2022Quote: ... and it was centrifuged at 400 g for 5 min to remove any cells before filtering through a cellulose acetate membrane with 0.45 μm pore size (Millipore). The supernatant was snap-frozen in dry ice before storing it at -80°C ...
-
bioRxiv - Neuroscience 2022Quote: Isolated retinas were cut into four-leaf clovers and transferred onto filter paper (0.45 mm nitrocellulose membranes, MF-Millipore) with the ganglion cell layer facing up ...
-
bioRxiv - Cancer Biology 2022Quote: ... The transfer used a wet transfer protocol similar to that used for Western blot described previously but using 0.5X TBE buffer as transfer buffer and transferring to Immobilin Ny+ nylon membrane (Millipore). Membrane was put on paper towel to dry briefly ...
-
bioRxiv - Biochemistry 2023Quote: Equal amounts of protein from cell lysates were subjected to SDS-PAGE and transferred to a PVDF membrane (Millipore). HeLa GFP and HeLa TRAP1-GFP cells were treated with either 2 μg/mL of harringtonine and 100 μg/mL of emetine for 15 min at 37°C to inhibit cytosolic translation or 30 μM linezolide or 200 ng/mL chloramphenicol for 1 hr at 37°C to inhibit mitochondrial translation ...
-
bioRxiv - Microbiology 2023Quote: ... was subjected to centrifugation through a 3 kDa cut-off membrane filter (Amicon Ultra-0.5 3-kDa Ultracell, Millipore) at 4 °C ...
-
bioRxiv - Microbiology 2022Quote: ... Different amount of protein samples were electrophoresed on 10% SDS-PAGE and then transferred to PVDF membranes (Millipore, Merck) by electroblotting ...
-
bioRxiv - Microbiology 2022Quote: ... Membranes were washed thrice and bound antibodies were detected by using Immobilon ECL Ultra Western HRP Substrate (Merck Millipore) on the ChemiDoc XRS+ system.
-
bioRxiv - Microbiology 2022Quote: ... After loading the mixed solution into 96-well MultiScreen Filter Plates (product number MSHVN4510, 0.45 μm hydrophilic PVDF membrane, Millipore), filtration was performed by centrifugation ...
-
bioRxiv - Molecular Biology 2023Quote: ... for 90 min at 120 V and blotted at constant 30 V for 30 min onto 0.45 µm Immobilon-FL PVDF membrane (Merck Millipore). After the transfer ...
-
bioRxiv - Neuroscience 2023Quote: ... slices were transferred onto culture membranes (uncoated 30 mm Millicell-CM tissue culture inserts with 0.4 µm pores, Millipore) and kept in six-well culture dishes (BD Biosciences) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Proteins were then transferred to a PROTRAN 0.2 µm nitrocellulose membrane (Cytiva) and stained with Ponceau S (Sigma-Aldrich). Membranes were incubated in 5% (w/v ...
-
bioRxiv - Cell Biology 2023Quote: ... An equal amount of protein was analyzed using SDS-PAGE and transferred to polyvinylidene fluoride PVDF membranes (0.22 μm pore size, Millipore). The membranes were blocked with Odyssey Blocking Buffer (LI-COR ...
-
bioRxiv - Immunology 2023Quote: ROS production in BMDMs was measured using the membrane-permeable fluorescent probe 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFHDA) (Sigma, #D6883). BMDMs were plated in 96-well plates at 1×105 cells/well ...
-
bioRxiv - Developmental Biology 2023Quote: ... DNA was purified by the drop dialysis method using the MF-Millipore Membrane Filter (Merck Millipore, Cat. No. #VCWP04700). Length distribution of the genomic DNA was analyzed by pulsed-field gel electrophoresis ...
-
bioRxiv - Biochemistry 2023Quote: ... membranes were blocked with 5% BSA in TSMT (20 mM Tris; 150 mM NaCl, Merck; 1 mM CaCl2, Sigma; 2 mM MgCl2 ...
-
bioRxiv - Molecular Biology 2023Quote: ... The lysate was sequentially filtered through a tangential flow filtration cassette with 0.45 µm pore size (PVDF membrane; Pellicon XL 50, Millipore) and concentrated on a 1,000 kDa MWCO tangential flow filtration cassette (regenerated cellulose membrane ...
-
bioRxiv - Cell Biology 2023Quote: ... 15 μl of each sample was resolved by 9% SDS-PAGE and transferred to a PVDF membrane (Millipore IPFL00010) and blotted using an anti-EYFP antibody (Thermo MA5- 15256 ...
-
bioRxiv - Cell Biology 2022Quote: ... Both types of gel were used throughout study and gels were transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, #IPVH85R). The membrane was blocked in 5 % milk-TBST (150 mM NaCl ...
-
bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of total proteins (20μg) were separated by SDS-PAGE and transferred to a 0.45-mm Immobilon-P PVDF Membrane (Millipore, Merck) and the membrane was incubated with primary antibodies overnight at 4°C ...
-
bioRxiv - Cancer Biology 2022Quote: ... The total protein was then resolved on SDS-PAGE (8-12%) and transferred onto PVDF membrane (Millipore, Massachusetts, USA). After the transfer ...
-
bioRxiv - Biophysics 2023Quote: ... The beads were washed 3 × 1 mL with membrane resuspension buffer for KRas before the beads were eluted with membrane resuspension buffer supplemented with 0.2 mg/mL 3X FLAG peptide (SAE0194, Sigma). Aliquots of samples were collected at each stage (input to anti-FLAG resin ...