Labshake search
Citations for Millipore Sigma :
6601 - 6650 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ALT and AST activity was determined in serum with the commercially assay kits (Sigma-Aldrich, MAK052; and MAK055) following manufacturer’s recommendations.
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bioRxiv - Biochemistry 2020Quote: ... Incubation with kit-specific secondary antibodies (anti-rabbit MINUS Sigma Cat. DUO92005, anti-goat PLUS Sigma Cat. DUO92003), ligation ...
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bioRxiv - Neuroscience 2021Quote: ... C-reactive protein (CRP) was measured by an ELISA kit (cat. number CYT294, Millipore Sigma, Saint Louis, MO) following the manufacturer’s instructions.
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bioRxiv - Plant Biology 2020Quote: Total RNA was extracted from roots (cut at about 0.2-0.5 cm from the tip) using the Spectrum plant total RNA kit (Sigma). RNA sequencing and analysis was performed at the Technion Genome Center (LSE) ...
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bioRxiv - Plant Biology 2021Quote: ... and total RNA was extracted with the Spectrum™ Plant Total RNA Kit (Sigma Aldrich, Saint Louis, USA). The isolated RNA was treated with deoxyribonuclease I enzyme (Sigma Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... RNA was extracted from 100 mg of frozen tissue using the Spectrum total RNA plant kit (Sigma-Aldrich). The mRNA library was prepared by poly-A enrichment and paired-end sequencing was performed on Illumina NovaSeq6000 (2 × 150 bp ...
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bioRxiv - Genomics 2021Quote: The total RNA of each sample was isolated using the Spectrum™ Plant Total RNA kit (Sigma, USA). Seedlings (shoot and root ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... AmiconUltra-0.5 centrifugal filter (UFC5010) DNase I Kit (AMPD1) were purchased from Sigma-Aldrich (St. Quentin Fallavier, France). NuleoSpin® RNA/Protein extraction kit (740933.50 ...
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bioRxiv - Plant Biology 2021Quote: RNA was extracted from three biological replicates per genotype using the Spectrum Plant Total RNA Kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The cell lysate was used to perform ALP activity assays using the ALP activity kit (Sigma-Aldrich, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The pJFRC19 backbone and TkR86C fragments were ligated using Roche Rapid DNA Ligation Kit (Millipore Sigma cat#11635379001). The recovered TkR86C coding sequences (isoform B ...
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bioRxiv - Cancer Biology 2020Quote: Confluent cells were trypsinized and stained by PKH26 Red Fluorescent Cell Linker Kit (Sigma-Aldrich, Cat No. PKH26GL) following the vendor’s instruction ...
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bioRxiv - Cancer Biology 2020Quote: Glucose measurements in the cell culture medium were performed using the Glucose Assay Kit (Sigma-Aldrich, GAGO-20). In brief ...
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bioRxiv - Cancer Biology 2021Quote: ... followed analysis of cell surface annexin-V and cell permeability using the Annexin-V-FLUOS staining kit (Sigma). Flow cytometry was performed on live cells using a BD FACSCalibur cytometer at the McGill Life Sciences Complex Flow Cytometry Facility.
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bioRxiv - Cancer Biology 2021Quote: ... A leukocyte acid phosphatase kit (#387A) for tartrate-resistant acidic phosphatase (TRAP) staining was purchased from Sigma-Aldrich. LipoD293 DNA in vitro transfection reagent (#SL100468 ...
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bioRxiv - Molecular Biology 2022Quote: ... The experiment was conducted with EZ-Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore,17-700 Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The experiment was conducted with EZ-Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore,17-700 Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Chitinase activity of cell free supernatants was measured using a commercially available fluorometric chitinase assay kit (Sigma-Aldrich) following the protocol ...
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bioRxiv - Immunology 2022Quote: The ecCa2+ concentrations were measured spectrophotometrically using calcium colorimetric assay kit from Sigma-Aldrich (Sigma-Aldrich, MAK022-1KT). ecCa2+ levels were determined as follows ...
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bioRxiv - Neuroscience 2022Quote: ... HRP staining was done by incubating the sections in a solution containing Diaminobenzidine reaction (DAB kit, Sigma-Aldrich) and a Nissl counterstain ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA was reverse transcribed to cDNA using the Transcriptor High Fidelity cDNA Synthesis Kit (#5081963001, Millipore Sigma) and stored at -20°C until analysis ...
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bioRxiv - Genetics 2022Quote: Proximity Ligation Assays were performed with the Duolink In Situ Red Starter Kit Mouse/Rabbit (Sigma-Aldrich, DUO92101). Cells on coverslips were fixed in 2% PFA for 12 minutes at RT°C and then permeabilized in PBS 0,2 % Triton X-100 for 5 minutes at RT°C ...
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bioRxiv - Physiology 2022Quote: ... PLA was proceed using the Duolink In Situ Red Starter Kit Mouse/Rabbit (catalog no. DUO92101, Sigma-Aldrich) following manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... coupled to a MINUS probe using the Duolink In Situ Probemaker MINUS kit (catalog no. DUO92010, Sigma-Aldrich). Images were captured with a LSM 780 confocal microscope (ZEISS).
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bioRxiv - Microbiology 2022Quote: ... salivarius DPC6487 culture cell pellets using a GenElute™ Bacterial Genomic DNA Kit (Sigma-Aldrich; Co. Wicklow, Ireland). The purity and concentration of genomic DNA was confirmed using the NanoDrop 1000 (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... The crude RNA extract was further purified using the GenElute™ Mammalian Total RNA Miniprep kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: An RIP assay was performed using a Magna RIP RNA binding protein immunoprecipitation kit (17-700; Merck Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration was measured using Pierce™ BCA Protein Assay Kit (Sigma-Aldrich, St. Louis, Missouri United States) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA from individual larva of Tribolium was extracted using the GenElute™ Mammalian Total RNA kit (Sigma). cDNA synthesis was carried out as previously described [72,73] ...
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bioRxiv - Cell Biology 2021Quote: ... The phosphatase assay was carried out using the Ser/Thr Phosphatase Assay Kit 1 (Millipore Sigma (MA, USA)) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... PLA was performed using Duolink® In Situ Red Starter Kit Mouse/Rabbit (Sigma-Aldrich cat. DUO92101-1KT) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Blood levels of TAGs were measured by a colorimetric assay using the Triglyceride determination kit (Sigma-Aldrich, #MAK266). LCN2 plasma levels were determined by ELISA (R&D Systems ...
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bioRxiv - Physiology 2021Quote: ... we employed Masson-trichrome staining using the Accustatin Trichrome Statin Kit (#HT15-1KT; Sigma-Aldrich, St. Louis, MO) in accordance with the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... solium larval extracts were assessed for protein concentration using a BCA or Bradford protein assay kit (Sigma-Aldrich), respectively.
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bioRxiv - Microbiology 2021Quote: ATPase activity of LolCDE in DDM detergent or nanodiscs was measured using a colorimetric ATPase kit (Sigma Aldrich) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were washed with buffer A from the Duolink II proximity ligation assay kit (Olink Bioscience, Sigma), followed by the addition of Duolink II PLA probe anti-mouse Minus and Duolink II PLA probe anti-rabbit Plus ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Snap frozen lungs were homogenized and nuclear extracts were obtained using the CelLyticTM NuCLEARTM Extraction Kit (Millipore-Sigma). Protein concentrations were determined by Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: RIP assay and RNA pull down assay were performed using RIP RNA-Binding Protein Immunoprecipitation kit (Sigma, USA) and Magnetic RNA-Protein Pull-Down Kit (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... An Ellman assay (Ellman et al., 1961) was then performed using an Acetylcholinesterase activity assay kit (Sigma-Aldrich). Absorbance was read at 412 nm every minute for 10 minutes using a VersaMax (Molecular Devices ...
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bioRxiv - Genetics 2022Quote: ... 50 thoraces per group were dissected and single nuclei were prepared using the nuclei EZ isolation kit (Sigma) according to manufacturer instructions ...
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bioRxiv - Systems Biology 2022Quote: ... the amounts of glycerol in the supernatant and fresh medium was measured using glycerol assay kit (Sigma Aldrich) by a coupled enzyme assay involving glycerol kinase and glycerol phosphate oxidase ...
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bioRxiv - Evolutionary Biology 2022Quote: ... total genomic DNA was isolated using the GenElute Plant Genomic DNA Miniprep kit (Sigma-Aldrich, St. Louis, MO) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: Multiplex cytokine analysis on PBMC culture supernatants were performed using Milliplex multiplexed cytokine/chemokine panel kits (EMD Millipore) and a Luminex FlexMAP 3D instrument with the xPONENT version 4.0 software ...
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bioRxiv - Developmental Biology 2022Quote: Proximity Ligation Assay (PLA) was performed using the Duolink In Situ Red Started Kit Mouse/Rabbit (Sigma Aldrich) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: The total RNA was extracted using Spectrum™ Plant Total RNA kit as per manufacturer’s protocol (Sigma-Aldrich). The qRT-PCR was performed with mixture of 2 μL of primer mix (2 μM from each F & R primer) ...
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bioRxiv - Microbiology 2019Quote: ... Cell-free extracts were then tested for trehalose levels according to the Glucose Assay Kit protocols (Sigma AGO20). Results from biological triplicate experiments were averaged ...
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bioRxiv - Microbiology 2019Quote: ... The cell lysate was used to perform ALP activity assays using the ALP activity kit (Sigma-Aldrich, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Pellets were processed according to the NXTRACT kit (CelLytic™ NuCLEAR™ Sigma-Aldrich, Milan, Italy, protocol B), to obtain native and functional nuclear proteins ...
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bioRxiv - Neuroscience 2019Quote: ... large-scale DNA plasmid purification was done with GenElute HP Endotoxin-Free Plasmid Maxiprep Kits (Sigma-Aldrich Corp.).
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bioRxiv - Cancer Biology 2019Quote: G6PD activity assay was performed on CD133hi and CD133lo cells using the G6PD activity assay kit (Sigma-Aldrich) according to manufacturer’s protocol.