Labshake search
Citations for Millipore Sigma :
6551 - 6600 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... agarose was washed in a stepwise fashion with solutions from a Chromatin Immunoprecipitation Assay Kit (Millipore Sigma), beginning with 1 mL of a low salt wash buffer ...
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bioRxiv - Immunology 2024Quote: ... and the concentrations of extracellular L-lactate were measured using the L-Lactate Assay Kit (Sigma-Aldrich), following the manufacturer’s instructions.
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bioRxiv - Physiology 2024Quote: ... Muscle glycogen concentrations were then quantified from the supernatant using a commercially available kit (Sigma-Aldrich-MAK016) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... in 1mL nuclei lysis buffer using the Nuclei Pure Prep Nuclei Isolation Kit (Sigma Adlrich, NUC-201). 3mL lysis buffer was added and incubate on ice for 15 minutes ...
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bioRxiv - Biophysics 2024Quote: The membrane fraction was isolated from Neuro2A cells using the ProteoExtract® Subcellular Proteome Extraction Kit (Sigma) and concentrated to 1-2 mg/mL using a PierceTM concentrator (3K molecular weight cutoff ...
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bioRxiv - Biophysics 2024Quote: The ATPase activities of MDA5 and LGP2 were measured utilizing an ATPase/GTPase Activity Assay Kit (Sigma). The analysis was carried out with 200 nM protein and 100 ng RNA substrate (for ssRNA ...
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bioRxiv - Biochemistry 2024Quote: ... Development was carried out using the ECL Western blot substrate kit according to the manufacturer’s instructions (Millipore). The biotinylated inner membrane-associated protein MmdC was used as a loading control (48 ...
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bioRxiv - Developmental Biology 2024Quote: PLA was performed according to the Duolink In Situ Red Starter Kit Mouse/Rabbit (Sigma-Aldrich, DUO92101) protocol with some modifications ...
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bioRxiv - Immunology 2024Quote: ChIP experiments were strictly performed according to the manual for the ChIP Assay Kit (#17-10086, Millipore) and the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: Glycogen levels in total hepatocytes were measured by using the glycogen assay kit (Sigma-Aldrich, #MAK016-1KT) according to the manufacture’s protocol.
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bioRxiv - Biochemistry 2024Quote: ... We measured the amounts of IgE in the serum with a commercially available ELISA kit (Sigma-Aldrich).
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bioRxiv - Evolutionary Biology 2024Quote: ... including on-column DNA digestion using the on-column DNAse kit (Sigma-Aldrich, product number: DNASE70-1SET). The Agilent 2100 bioanalyzer (Agilent ...
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bioRxiv - Microbiology 2024Quote: Sialic acid (SA) concentration was determined in mucus samples using a SA assay kit (Sigma-Aldrich, MAK314) and following the manufacturer protocol ...
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bioRxiv - Neuroscience 2024Quote: Proximity ligation assay (PLA) was carried out using either the Duolink PLA kits for brightfield (Sigma, #DUO92012) or fluorescent applications (Sigma ...
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bioRxiv - Plant Biology 2020Quote: ... The protein amount of the resuspended membrane fractions was quantified by Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich) and equilibrated between different samples ...
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bioRxiv - Cancer Biology 2021Quote: ... Fluorescent β-Galactosidase was performed according to the manufacturer’s instructions using the following commercial kit (Sigma-Aldrich, #F2756). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: Fractionation of intact nuclei from cytoplasm was performed using Nuclei Isolation Kit: Nuclei EZ Prep (Sigma, #NUC101-1KT) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Hexokinase activity was assayed in cell homogenates using the Hexokinase activity kit (MAK091, Sigma, St. Louis, MO, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Total intracellular glutathione content (reduced plus oxidised glutathione) was measured using a colorimetric glutathione assay kit (Sigma CS0260), measured by absorbance at 412 nm (Versamax microplate reader Molecular Devices).
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bioRxiv - Cell Biology 2020Quote: ... Mitochondria isolated were then subjected to reactive oxygen species (ROS) assay using fluorometric intracellular ROS kit (Sigma, USA). The fluorescence was acquired with excitation and emission wavelengths of 490 nm and 520 nm ...
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bioRxiv - Immunology 2021Quote: ... Cholesterol and cholesteryl esters were measured on isolated lipid fraction by using Cholesterol/Cholesteryl Ester Quantitation Kit (Sigma) according to the supplieŕs protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: RNA isolation from uninucleated microspores was performed by using the Spectrum™ Plant Total RNA Kit (Sigma-Aldrich) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... RNA extraction was carried out with the Spectrum Plant Total RNA Mini Kit (Sigma-Aldrich, St. Louis, MO) or the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Developmental Biology 2020Quote: Proximity ligation assay was performed according to the instructions of the PLA Duolink® assay kit manual (Sigma). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: Proximity ligation assays were carried out with Duolink® In Situ Red Starter Kit Mouse/Rabbit (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... and Monarch RNA Cleanup Kit T2030L) in combination with phenol:chloroform prepared from molecular grade reagents (Sigma Aldrich, USA), and TRIzol reagent (Thermo Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... genomic DNAs were isolated from clonal populations using GenElute(tm) Mammalian Genomic DNA Miniprep Kit Protocol (Sigma-Aldrich). A genomic region of RIOK2 gene encompassing Ser483 was PCR-amplified from the genomic DNAs ...
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bioRxiv - Physiology 2019Quote: ... The silver stained protein spots were de-stained using the ProteoSilver Plus Stain Kit (Sigma-Aldrich, MO, USA), and in-gel enzymatic digestion was done overnight at 37°C using Trypsin Singles ...
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bioRxiv - Physiology 2019Quote: ... Gels containing protein spots were then stained using the ProteoSilver Plus Silver Stain Kit (Sigma-Aldrich, MO, USA) according to manufacturer instructions ...
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bioRxiv - Physiology 2019Quote: ... Citrate synthase activity was measured on a 96-well plate using a commercially available kit (Sigma Aldrich, CS0720).
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bioRxiv - Plant Biology 2020Quote: ... RNA was extracted from 4-week-old Arabidopsis leaves using the Spectrum plant total RNA kit (Sigma-Aldritch) according to manufacturer’s instructions.
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bioRxiv - Plant Biology 2020Quote: ... Total RNA was extracted from both seed and seedling samples using the Spectrum Plant RNA kit (Sigma-Aldrich), including an on-column DNase step ...
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Glutathione S-transferase: A Candidate Gene for Berry Color in Muscadine Grapes (Vitis rotundifolia)bioRxiv - Genetics 2020Quote: ... RNA was isolated from berry skins using Spectrum™ plant total RNA kit (Sigma-Aldrich, St. Louis, MO) according to the manufacturer’s instructions and from the seeds as described by Reid et al ...
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bioRxiv - Genetics 2019Quote: ... 10 μL of supernatant was used to measure triglyceride by using Serum TG determination kits (Sigma, TR0100-1KT). 10 μL supernatant was treated with 0.2 μL water or trehalase (Megazyme ...
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bioRxiv - Cancer Biology 2020Quote: RNA was extracted from cell lines and homogenized tumors using a GenElute Mammalian Total RNA Purification Kit (Sigma) according to standard protocols ...
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bioRxiv - Molecular Biology 2019Quote: Paraffin-embedded sections were stained with either a Periodic acid Schiff (PAS) staining kit (Sigma-Aldrich, United States), a Trichrome (Masson ...
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bioRxiv - Genomics 2021Quote: ... Libraries were then treated with sodium bisulphite according to the manufacturer’s instructions (Imprint DNA Modification Kit; Sigma, MOD50) and amplified by PCR (10 cycles ...
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bioRxiv - Developmental Biology 2021Quote: ... and testosterone levels were measured using enzyme-linked immunosorbent assay kits (progesterone, Biovision, K7416-100; estrogen-17β, Sigma, ab108667 ...
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bioRxiv - Neuroscience 2020Quote: ... Glycerol content was assessed using a free glycerol determination kit with glycerol standard solution (Sigma-Aldrich, MO, USA). Final glycerol content and NEFA-C levels were expressed relative to the weight of the respective epididymal WAT explant.
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bioRxiv - Cancer Biology 2020Quote: Chromatin immunoprecipitation (ChIP) was carried out as described in the Acetyl-Histone H3 Immunoprecipitation Assay Kit by Millipore. DNA recovered from chromatin immunoprecipitation was analyzed by real-time PCR ...
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bioRxiv - Developmental Biology 2020Quote: ... DIG-labeled RNA probes were generated using the DIG RNA Labeling Kit SP6/T7 (Sigma-Aldrich cat. # 11175025910) according to manufacturer’s protocols ...
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bioRxiv - Bioengineering 2021Quote: ... Fractionation of the nuclear protein was performed according to the Pierce cytoplasmic and nuclear extraction kit instructions (Sigma). Samples were run on an SDS-Page gel (BioRad ...
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bioRxiv - Biochemistry 2020Quote: ... and enzyme activity assay kit for β-N-Acetyl-glucosaminidase (NAG) were purchased from Sigma (St. Louis, MO). Recombinant NAG enzyme was from New England Biolabs (Beverly ...
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bioRxiv - Biophysics 2021Quote: ... This new pellet was resuspended in CellVue Claret membrane kit-staining-component Diluent C (Sigma-Aldrich, Miniclaret-1kt). Staining of membranes took place according to manufacturer’s instructions and was stopped by adding 2% bovine serum albumin ...
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bioRxiv - Developmental Biology 2020Quote: In vitro transcription of digoxigenin-labelled probes was done with DIG RNA-labeling kit (Sigma-Aldrich, Cat # 11175025910). The following digoxigenin-labelled probes were synthetized in the lab from cDNA ...
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bioRxiv - Neuroscience 2021Quote: ... Total RNA was reverse transcribed to cDNA with the Transcriptor High Fidelity cDNA Synthesis Kit (#5081963001, Millipore Sigma) using the manufacturers protocol ...
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bioRxiv - Molecular Biology 2021Quote: Nuclei from human liver (15 mg) were isolated using with the Nuclei EZ Prep kit (Sigma-Aldrich, #NUC101) and a glass dounce homogenizer (25 strokes with lose pastel and 25 strokes with tight pastel in ice-cold Nuclei EZ Lysis Buffer) ...
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bioRxiv - Neuroscience 2020Quote: Chromatin for ChIP was prepared from immortalized human microglia using the Imprint® Chromatin Immunoprecipitation kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The plasmid was linearized and subjected to in vitro transcription with digoxigenin labeling (DIG RNA labeling kit, Sigma). Sections were hybridised with 500 ng/ml riboprobe in hybridization solution at 72°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... The NH4Cl concentrations in the growth media were determined using the Aquaquant ammonium quantification kit (Merck Millipore, UK) as per the manufacturer’s instructions ...