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Citations for Millipore Sigma :
6501 - 6550 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Samples were run on 12% SDS-polyacrylamide gels and subsequently transferred to 0.2 μm polyvinylidene difluoride membrane (PVDF) (Millipore). Membranes were washed with 1X TBS and incubated with a 5% solution of BSA for 1 hr at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... lysed in Laemmli SDS-loading dye and separated in 15% acrylamide gels and transferred to 0.45 μm hydrophobic Immobilon-P PVDF membrane (Merck Millipore). Immunoblotting was as described previously 19 ...
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bioRxiv - Microbiology 2020Quote: ... cells were stimulated with pools of S1 or S2 peptides (final concentration of 2μg/ml) on IPVH-membrane plates (Millipore) coated with 5μg/ml anti-mouse IFN-γ (AN18) ...
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bioRxiv - Neuroscience 2021Quote: ... Punches were placed into 100 μm-deep membrane carriers and tissue was surrounded with 20% bovine serum albumin (Sigma) in cacodylate buffer to prevent air pockets ...
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bioRxiv - Microbiology 2020Quote: ... 1 OD600*mL of the donor was added to 1 OD600*mL of the recipient community or isolate and the mixture was plated on a 0.45 μm mixed cellulose ester membrane (Millipore) topping a plate of the recipient’s preferred media without DAP ...
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bioRxiv - Neuroscience 2022Quote: ... Each protein sample was electrophoresed on 10% (w/v) SDS-polyacrylamide gel electrophoresis (PAGE) and subsequently transferred onto 0.22 μm polyvinylidene difluoride (PVDF) membranes (Millipore). Then ...
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bioRxiv - Cancer Biology 2022Quote: ... the protein extracts (15 μg/sample) were blotted on a primed 0.45 μm polyvinylidene fluoride (PVDF) membrane (Merck-Millipore). Protein spots were stained using Direct Blue (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins samples (20 μg/lane) were separated by SDS–PAGE and transferred onto PVDF membranes (0.45μm, Millipore, USA) using a transfer unit (Bio–RAD ...
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bioRxiv - Microbiology 2022Quote: ... The blocked membrane was incubated with the primary antibodies: mouse anti-HA (2,000×, Sigma-Aldrich HA-7, Massachusetts, USA), mouse anti-α-actin (20,000× ...
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bioRxiv - Genomics 2022Quote: ... Proteins from whole cell lysate were separated in Laemmli buffer by SDS-PAGE and transferred to PVDF membranes (Millipore). Membranes were blocked in 5% milk in Tris-buffered saline with 0.1% Tween-20 (TBST ...
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bioRxiv - Neuroscience 2022Quote: ... 25 μg of protein were resolved by 8% dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and then electrophoretically transferred to a 0.45 μm polyvinylidene difluoride membrane (PVDF; Immobilon, Merck Millipore). Membranes were blocked in blocking buffer (5% non-fat milk or 5% FBS diluted in TBS-T buffer (0.05% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Systems Biology 2022Quote: Protein samples were subjected to SDS-PAGE (14% gel) and wet-transferred to a 0.2 μm Immobilon-PSQ PVDF membrane (Millipore) at 110 V for 90 min ...
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bioRxiv - Immunology 2022Quote: ... Protein lysates were separated on 10% SDS-PAGE gels and then transferred to PVDF membranes (Millipore, Billerica, MA, USA). The membranes were blocked with 5% nonfat dry milk at room temperature for 2 hours and then incubated at 4°C overnight with primary antibodies against β-tubulin (Abcam ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was dried overnight at RT and blocked for 30min with 2%(W/V) BSA in PBS (Sigma), incubated while shaking ON at 4°C with rabbit anti-Histone H3(Abcam ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell lysates were subjected to SDS-polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride membranes (Millipore Corporation, Billerica, MA). After blocking with 5% milk or 5% BSA in Tris-buffered saline/0.1% Tween for 1 h ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were resolved on 12% SDS–PAGE and proteins were transferred to methanol-activated polyvinylidene fluoride (PVDF) membrane (Millipore) using anode buffer (25 mM Tris HCl pH 10.4 ...
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bioRxiv - Biochemistry 2020Quote: ... A 96-well dot blot apparatus (Schleicher & Schuell) was set up with a 0.45-μm-pore-size polyvinylidene difluoride (PVDF) membrane (Immobilon-P; Millipore), and each dot was rinsed with 500 μl of TBS ...
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bioRxiv - Physiology 2020Quote: ... Membranes were probed overnight at 4°C with anti-phosphotyrosine antibody (1/1000, 05-321, clone 4G10, Merck Millipore) in blocking solution ...
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bioRxiv - Immunology 2020Quote: ... denatured cell lysates were separated by 10% SDS polyacrylamide gel electrophoresis and transferred to a polyvinylidene fluoride membrane (Millipore) for probing with specific primary antibodies and HRP-conjugated secondary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... Fractions 7-9 were individually collected (500 μL/fraction) and concentrated by Amicon Ultra-0.5 centrifugal filter unit with 10 kDa membrane (Merck Millipore) with 14,000 x g at 4 °C for 8 min centrifugation to have final volume less than 100 μL ...
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bioRxiv - Pathology 2020Quote: ... cell lysates containing equal protein amounts were separated by SDS-PAGE and transferred to PVDF membranes (Cat.#ISEQ00010, Millipore). Membranes were sequentially probed with primary antibodies and appropriate peroxidase-conjugated secondary antibodies ...
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bioRxiv - Microbiology 2020Quote: ... 24-hr old biofilms were formed on sterilized polycarbonate membrane filters with a 0.2 µM pore size (Millipore Sigma) by diluting an overnight culture to an OD600 of 0.001 and pipetting 100 µl on 4 membranes on a tryptic soy agar (TSA ...
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore, Merck KGaA, Darmstadt, Germany) diluted in 2 % skim milk for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysate was cleared by centrifugation at 40,000 x g for 30 min at 4°C and the supernatant filtered through a syringe-driven 0.45-μm PVDF membrane (Millipore). To prepare the beads ...
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bioRxiv - Molecular Biology 2020Quote: ... The samples were electrophoresed on denaturing SDS-polyacrylamide gels and transferred onto Immobilon-P PVDF membranes (Merck Millipore, USA). The membranes were then blocked using 5% non-fat milk in PBS for 30 minutes followed by overnight incubation at 4°C with primary antibody against protein of interest at the desired dilution in 2% non-fat milk prepared in PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... Proportional volumes of bacterial and yeast cells were mixed and filtered through 0.45 µm mixed cellulose membrane (Merck, Millipore) before transferring onto IM agar supplemented with 200 µM acetosyringone ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein samples were separated by 10% SDS-PAGE gel and then electrically transferred to 0.45μm PVDF membranes (Millipore, IPVH00010). Membranes were then blocked with 5% non-fat milk in TBST for one hour at room temperature ...
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bioRxiv - Immunology 2020Quote: ... was then used to block empty sites on the AuNP and filtered via a 0.22 μm PVDF membrane (Millipore). Coated particles were subsequently added to the test antibody solution and incubated for 2 hours at RT before measuring absorbance from 510 to 570 nm on a plate reader ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were blocked with 5% nonfat milk in phosphate-buffered saline with 0.1% Tween 20 (Sigma-Aldrich, P9416; PBST). The blots were incubated overnight at 4°C with primary antibodies ...
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bioRxiv - Microbiology 2020Quote: ... US28-expressing membrane extracts were coated in a 96 well MicroWell™ MaxiSorp™ flat bottom plate (Sigma-Aldrich) overnight at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... The polyvinylidene difluoride (PVDF) membranes were incubated overnight at 4°C with appropriate antibodies: anti-Arp (dilution 1:1,000; Sigma); anti-FHOD1 (dilution 1:1,000 ...
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bioRxiv - Microbiology 2020Quote: ... The culture medium containing the secreted proteins was harvested by centrifugation (5000 g for 20 min at RT) and filtered through a 0.22 µm membrane filter (Millipore). Recombinant proteins were purified by immobilized metal affinity chromatography (IMAC ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted fraction containing purified bacteriocin was concentrated to 4.6 mg/mL in an Amicon centrifugal filter concentrator with a 3 kDa cutoff membrane (Millipore) in buffer comprising 20 mM sodium phosphate (pH 6 ...
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bioRxiv - Neuroscience 2020Quote: ... 5μg of total cell lysate proteins were resolved on 4-12% SDS-PAGE and transferred onto 0.45 μm polyvinyl difluoride membrane (Millipore) as described above under western blotting of EVs.
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bioRxiv - Neuroscience 2020Quote: ... the membrane was incubated for 30 minutes at room temperature with anti β-actin (1:10000, A3854 Sigma-Aldrich), wich is used as a normalizer ...
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bioRxiv - Immunology 2021Quote: ... and two pools of S2 peptides (pools 3 and 4) (final concentration of 2μg/mL) on IPVH-membrane plates (Millipore) coated with 5μg/mL anti-mouse IFNγ (AN18) ...
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bioRxiv - Immunology 2020Quote: ... donkey serum TBS IHC wash buffer and filtered using a centrifugal filter with a 0.1 mm PVDF membrane (Ultrafree-MC, Merck Millipore). Sections were stained with the antibody mix ...
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bioRxiv - Neuroscience 2020Quote: Organotypic hippocampal slices (350-µm) were prepared from male Wistar rats (P7) and cultured on Millicell CM membranes (Millipore). Slices were maintained in culture media (Life Technologies/Sigma Aldrich) ...
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bioRxiv - Physiology 2020Quote: ... Membranes were then washed using a commercial HRP Substrate solution (Immobilion Forte Western HRP, #WBLUF0100, Millipore Sigma, Oakville, Canada). After protein quantification ...
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bioRxiv - Microbiology 2021Quote: ... Extracellular proteins (10 μg/mL; totally 700 mL) were filtered through a 0.22-μm nitrocellulose membrane (47-mm diameter; Millipore) and concentrated using the Amicon Ultra Centrifugal Filters to 7 mL (cut-off of 30 kDa) ...
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bioRxiv - Biochemistry 2021Quote: ... adjusted to pH 5.8 using 3 M HCl and filtered with 0.2 μm nylon membrane filters (GNWP04700, 0.2 μm pore size, Merck Millipore Ltd.), while mobile phase B consisted of 100% methanol ...
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bioRxiv - Biochemistry 2021Quote: ... adjusted to pH 8 using 3 M HCl and filtered with 0.2 μm nylon membrane filters (GNWP04700, 0.2 μm pore size, Merck Millipore Ltd.), while mobile phase B consisted of 100% methanol (method described in Table 2) ...
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bioRxiv - Microbiology 2022Quote: ... and equal amounts of cell extracts were separated by SDS-12 % PAGE and transferred onto a nitrocellulose membrane (Sigma). Samples were then analyzed by western blot using the desired antibodies ...
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bioRxiv - Biochemistry 2020Quote: ... the membrane was washed four times with TTBS and secondary antibody goat-anti rabbit-HRP (catalog no. A7058, Sigma) was added at 1:60,000 in 5% milk for 2 hours at RT ...
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bioRxiv - Immunology 2020Quote: ... splenocytes were stimulated with S peptide pools at a final concentration of 2μg/ml on IPVH-membrane plates (Millipore) coated with 5μg/ml anti-mouse IFN-γ (clone AN18 ...
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bioRxiv - Neuroscience 2021Quote: ... The SC explants were quickly cut on a stereomicroscope and transferred to culture membranes (Millipore Millicell-CM, Bedford, MA) and cultured in a phenol-red-free Dulbecco’s modified eagle medium (DMEM ...
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bioRxiv - Biophysics 2021Quote: ... The mobile phase buffer consisted of 150 mM AMAC in water and filtered using a 0.22 µm disposable membrane cartridge (Millipore) before use ...
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bioRxiv - Immunology 2020Quote: ... concentrated and desalted 5X using a 10 kDa Amicon® 8400 Ultra filter membrane (Sigma Aldrich, St. Louis, MO). The purified and dialysed native CP extracellular protein was stored at −20 °C until further use.
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bioRxiv - Immunology 2020Quote: ... concentrated and desalted 5X using a 10 kDa Amicon® 8400 Ultra filter membrane (Sigma Aldrich, St. Louis, MO). The supernatant was collected as the cell wall protein fraction and stored −20 °C until further use.
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bioRxiv - Biophysics 2020Quote: Immunoprecipitation samples were fractionated on 6% or 15% SDS-polyacrylamide gels and transferred to polyvinylidene fluoride membranes (PVDF) (Millipore). Membranes were blocked in TBS solution (0.05% Tween-20 in PBS containing 5% milk) ...