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Citations for Millipore Sigma :
6551 - 6600 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Protein samples were separated on a 12.5% SDS-PAGE gel and transferred to a polyvinylidenedifluoride (PVDF) membrane (Immobilon-P, Millipore). Blots were blocked with 3% non-fat milk-0.05% Tween-20 in Tris buffer saline (TTBS ...
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bioRxiv - Microbiology 2021Quote: ... Lentiviral supernatants were harvested at 72 h post-transfection and filtered through a 0.22 μM membrane (Millipore, Burlington, MA). One day before transduction ...
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bioRxiv - Microbiology 2021Quote: ... proteins were separated using a 10% SDS-PAGE gel and transferred onto a PVDF Immobilon-P membrane (Merck Millipore). The membrane was blocked with 5% w/v milk in TBST (50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Microbiology 2021Quote: ... The viscous materials including cells after the second step of culture were collected by filtration using a 1.0-μm pore PTFE membrane (Millipore). The trapped materials were gently and carefully recovered from the membrane using MilliQ water with the aid of flat-tip tweezers ...
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bioRxiv - Microbiology 2020Quote: ... parvula at 105 cells mL−1 were placed into 50 mL conical tubes separated by a 0.22 μm membrane (Steriflip-GP, Millipore). Three conditions were tested ...
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bioRxiv - Biochemistry 2021Quote: ... Gels were then subjected to wet transfer (30% MeOH transfer buffer) of histones to 0.22 μm PVDF membranes (Millipore) and protein was detected by Western blot analysis using primary αRb H3K27me3 antibody (Millipore #07-449) ...
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bioRxiv - Microbiology 2020Quote: ... 70 L of groundwater were filtered onto a 0.1 μm pore-size PTFE membrane filter (Merck Millipore, Darmstadt, Germany). The flow-through was collected in a sterilized container ...
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bioRxiv - Immunology 2021Quote: ... peptide cocktails were combined with membrane disrupting antimicrobials effective against relevant pathogens beginning at: 10 μM Cecropin A (Sigma), 500μg/mL ampicillin ...
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bioRxiv - Microbiology 2021Quote: ... and the resulting total membrane fraction was homogenized in TBS buffer containing 1.5% Lauryl-dimethylamine oxide (LDAO) (Sigma, UK). Membrane proteins were extracted by stirring (60 minutes ...
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bioRxiv - Immunology 2020Quote: ... Semidry transfer was performed at 20 volts for 45 minutes onto a polyvinylidene difluoride (Immobilon-FL) membrane (EMD Millipore). Li-Cor Odyssey® blocking buffer (Li-Cor ...
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bioRxiv - Immunology 2020Quote: The assay for membrane depolarization activity of rBjRPS15 and BjRPS1545-67 was performed with the membrane potential-sensitive dye 3,3’-dipropylthiacarbocyanine iodide (DiSC3-5; Sigma) and the bacteria hydrophila ...
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bioRxiv - Immunology 2021Quote: ... The supernatant containing pseudo-virus was centrifuged at 3000g and filtered through a 0.45μm sterilized membrane (Millipore, Burlington, MA). The titer of virus generated by engineered Zhou-COVID-19-Spike plasmid is ten-fold higher than non-engineered Spike protein sequence (data not shown) ...
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bioRxiv - Cell Biology 2020Quote: Proteins were separated by electrophoresis on 12.5% SDS-polyacrylamide gels and then transferred to polyvinylidene difluoride (PVDF) membranes (Immobilon; Millipore) using a wet transfer tank (Hoefer Instruments) ...
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bioRxiv - Cell Biology 2021Quote: ... samples were cooled to room temperature and ∼50 µg of proteins were resolved on a 10% SDS-PAGE gel and transferred using a wet transfer setup onto a PVDF membrane with 0.45 µm pore size (Immobilon, Millipore). Membranes were blocked using Tris-buffered saline containing 5% nonfat dry milk and 0.1% Tween 20 (TBST) ...
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bioRxiv - Genetics 2020Quote: ... Protein samples were subjected to SDS-PAGE and wet-transferred to a 0.2 μM Immobilon-PSQ PVDF membrane (Millipore) at 200 mA for 90 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... IP samples were resolved on 8% or 10% SDS-PAGE gels and transferred onto Immobilon-P membranes (Merck Millipore) using a semidry transfer apparatus ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were blocked in Odyssey blocking buffer TBS (Licor) or 5% non-fat milk or 1% BSA (Sigma, A7906) for 1 hour before over-night incubation at 4°C with primary antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were run on SDS-polyacrylamide gels (SDS-PAGE) and then transferred onto a polyvinylidene difluoride (PVDF) membrane (Millipore). We blocked protein-containing membranes with Odyssey blocking buffer (LiCor ...
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bioRxiv - Neuroscience 2020Quote: ... and the retina was mounted on a nitrocellulose membrane (type AAWB, 0.8 micron pore size, Millipore, Burlington, MA, USA) and fixed by immersion in 4% paraformaldehyde for 30 mins ...
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bioRxiv - Physiology 2020Quote: ... Membranes were washed in TBS-T and proteins were visualized using a chemiluminescent substrate (Sigma Aldrich, Oakville, ON, Canada).
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bioRxiv - Microbiology 2022Quote: ... washed twice with ice-cold buffer A (50 mM Tris-HCl, 200 mM KCl, pH 8.0 at 4 °C, filtered through a 0.22-μM Millipore membrane), resuspended in buffer A with 20 mM imidazole added ...
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bioRxiv - Cancer Biology 2022Quote: Cell lysates were separated using Sodium dodecyl-sulfate polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride membranes (IPVH00010, Millipore) at 80V for 3h at 4°C ...
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bioRxiv - Physiology 2022Quote: ... onto PVDF membrane then blotted overnight at 4°C with anti-RBM20 antibody (Myomedix) or anti-γ-tubulin (Sigma) antibodies ...
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bioRxiv - Biophysics 2022Quote: ... Elutions were pooled down and concentrated using Vivaspin 6 centrifugal unit with 30 kDa cut off membrane (EMD Millipore) before being loaded onto a Superdex 200 increase 10/300 GL size-exclusion column (Cytiva ...
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bioRxiv - Biophysics 2022Quote: ... Elutions were pooled down and concentrated using Vivaspin 6 centrifugal unit with 30 kDa cut off membrane (EMD Millipore) before being loaded onto a Superdex 200 increase 10/300 GL size-exclusion column (Cytiva ...
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bioRxiv - Cancer Biology 2022Quote: ... were loaded onto a 10% or 12.5% SDS-PAGE gels and transferred to a polyvinylidene fluoride membrane (PVDF; Millipore-Sigma).
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bioRxiv - Biophysics 2022Quote: ... Purified Vnucfree solubilized in 0.03% n-DDM was mixed with the lipid stock and membrane scaffold protein MSP1E3D1 (Sigma) at a specific molar ratio of enzyme ...
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bioRxiv - Biochemistry 2022Quote: The enriched biomass in the liquid phase was filtered on a 0.22-μm pore size polycarbonate membrane (Millipore, USA) and a half was used for DNA extraction and the other half for RNA extraction ...
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bioRxiv - Cell Biology 2022Quote: ... gels were either fixed and stained with Coomassie Brilliant Blue R 250 or electrophoretically transferred onto nitrocellulose membranes (Millipore) by standard methods ...
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bioRxiv - Cell Biology 2022Quote: ... proteins were separated by electrophoresis through 12% polyacrylamide gels containing SDS (0.1%) and transferred to a PVDF membrane (Millipore). The membrane was blocked with 2% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... The viral supernatant was collected after 48 h and 72 h and filtered through a 0.45 µm PVDF membrane (Merck Millipore).
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bioRxiv - Genomics 2022Quote: ... Proteins from whole cell lysate were separated in Laemmli buffer by SDS-PAGE and transferred to PVDF membranes (Millipore). Membranes were blocked in 5% milk in Tris-buffered saline with 0.1% Tween-20 (TBST ...
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bioRxiv - Plant Biology 2022Quote: ... Membranes were then washed with TBST three times for 10min before incubating with a second antibody (anti-rabbit (Sigma), 1:2000 dilution ...
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bioRxiv - Microbiology 2022Quote: ... Plates were dried and wells were incubated with 50 µl of Tetramethylbenzidine (TMB) Liquid Substrate for Membranes (Sigma Aldrich) for 30 min at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... 40 μg total proteins were loaded into 10% acrylamide gels and transferred to Immobilon-P membrane (Millipore, Cat. IPVH00010). The antibody were diluted as follows ...
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bioRxiv - Neuroscience 2022Quote: ... Morphologically nice slices were carefully transferred using a plastic transfer pipette onto small pieces of PTFE membrane (Millipore FHLP04700) on membrane inserts (Millicell PICM0RG50 ...
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bioRxiv - Microbiology 2023Quote: ... The water samples were filtrated through 0,45 µm pore-sized cellulose membrane filters (Millipore Sigma Aldrich, St. Louis, MO) with a vacuum/pressure pump system (Pall Life Sciences ...
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bioRxiv - Plant Biology 2022Quote: ... Then total proteins were resolved in 8% SDS-PAGE and transferred to immobilon-P membrane (MILLIPORE cat. No. IPVH00010). GFP antibody (trans #HT801) ...
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bioRxiv - Microbiology 2023Quote: ... Culture volumes corresponding to OD600 of 10 were then harvested and rapidly filtered through a membrane (0.45 μm, Millipore). The cells on the membrane were washed twice with 5 ml cold saline and immediately quenched in ice-cold 60% ethanol containing 2 μM Br-ATP as an internal control ...
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bioRxiv - Physiology 2023Quote: ... membranes were exposed to overnight at 4°C with 1:15,000 anti-β-actin (Sigma Aldrich; Catalogue number: A5441) and for 1h at RT goat anti-mouse IRDye 680LT (LI-COR ...
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bioRxiv - Plant Biology 2023Quote: ... The resulting pellets were resuspended in distilled water and filter-sterilized through 0.22-μm-pore-size PVDF membrane filter (Millipore) before beening stored at −80°C.
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bioRxiv - Immunology 2022Quote: ... Protein extracts were loaded onto 8/15% polyacrylamide gels and then transferred onto a PVDF membrane (Millipore, Burlington, MA). Membrane was then blocked by incubating in 5% skim milk/TBS for 1 h at RT ...
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bioRxiv - Microbiology 2022Quote: ... The total membranes were solubilized with a buffer containing 1×PBS (pH 7.4) and 0.5% N-lauroylsarcosine sodium (Sigma-Aldrich) for 1 hr at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... followed by a 4000 g for 10 mins to further clarify the media before filtering through a 0.2 μm nylon membrane (Millipore). The media was then incubated with 1.5 mL of Ni-NTA beads (Qiagen ...
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bioRxiv - Neuroscience 2022Quote: Cultures fixed in 4% PFA were labelled after membrane permeabilization and saturation with 10% FBS and 0.02% Triton X-100 (Sigma) in PBS for 1 hour ...
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bioRxiv - Molecular Biology 2022Quote: ... by electrophoresis and subsequently transferred onto Immobilon-P™ PVDF membranes of 0.45 μm pore size (Millipore®; Merck). Details of primary antibodies used are provided in Supplementary Table 2 ...
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bioRxiv - Neuroscience 2022Quote: ... The resulting solutions were fractioned by 8–20% precast polyacrylamide gels and transferred to 0.2 µm pore-size PVDF membranes (ISEQ00010, Millipore). Blot membranes were heated for 5 minutes in PBS for epitope retrieving before blocking ...
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bioRxiv - Systems Biology 2022Quote: ... The membrane was rinsed with TBST (Tris-buffered saline [TBS] containing 0.1% Tween 20; catalog number P7949, Sigma-Aldrich) and blocked for 1 h with 5% BSA–TBS or 5% nonfat dry milk ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was washed three times in PBST and then developed by using Luminata Forte Western HRP substrate (Millipore).
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bioRxiv - Biochemistry 2024Quote: ... Equal amounts of lysate were loaded onto a 4–20% sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE) for electrophoresis and transferred to a 0.22 μm polyvinylidene fluoride (PVDF) membrane (Millipore). The membrane was blocked with 3 % bovine serum albumin in TBST buffer (20 mM Tris ...