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Citations for Millipore Sigma :
6451 - 6500 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Proteins were separated via gel electrophoresis on 7.5-12 % polyacrylamide gel and transferred to a PVDF membrane (Sigma Aldrich) using a semi-dry blotting system ...
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bioRxiv - Microbiology 2020Quote: ... the proteins in another gel were electrophoretically transferred to a methanol-activated polyvinylidene difluoride (PVDF) membrane (Millipore, Billerica, MA) and reacted with COVID-19 hospitalized patient sera (#12) ...
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bioRxiv - Microbiology 2020Quote: ... and membrane blocked overnight while shaking at 4°C in 5% milk (Marvel) containing 0.077% sodium azide (Sigma Aldrich). Primary antibodies were diluted in 5% milk/PBS/0.1% TWEEN 20 at concentrations as follows ...
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bioRxiv - Bioengineering 2020Quote: ... were cut with a 5100mz vibratome (Lafayette Instrument) and cultured on top of PFTE 0.4 µm transwell membranes (Millipore) in 6-well plates ...
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bioRxiv - Plant Biology 2021Quote: ... The solution was then dried using a speed vacuum (Centrivap concentrator, Labconco) and passed through a 0.2-mm membrane filter (Millex-GV filter unit, Merck Millipore). The filtrate was used for sucrose ...
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bioRxiv - Microbiology 2021Quote: ... The mobile phase buffer consisted of 150 mM AMAC in water and filtered using a 0.22 µm disposable membrane cartridge (Millipore) before use ...
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bioRxiv - Microbiology 2021Quote: ... 12 μg of protein was resolved by SDS-PAGE on a 17.5% acrylamide gel and transferred to an Immobilon-P membrane (Millipore). The membranes were blocked with 5% bovine serum albumin in 1XPBS with 0.5% Tween 20 (PBST ...
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bioRxiv - Neuroscience 2020Quote: ... Primary rat hippocampal neurons were dissociated from E18 Sprague-Dawley rat embryos (Janvier labs) and plated on the SN membranes previously treated with 1 mg.ml-1 poly-lysine (Sigma) in a 0.1 M borate buffer pH 8.5 ...
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bioRxiv - Neuroscience 2020Quote: ... SCN tissue was isolated from 300 µm slices prepared using a McIlwain Tissue Chopper (RRID:SCR_015798) and cultured on a Millicell filter membrane (Millipore, RRID:SCR_015799) in 1 ml culture medium ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Liposomes were frozen and thawed in liquid nitrogen for 10 rounds and extruded through 0.4 μm polycarbonate membrane (Millipore). 200 μg/ml purified proteins were added into liposomes after incubation with 1% n-octyl-ß-D-glucopyranoside (ß-OG ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were separated by 12% SDS-polyacrylamide gel electrophoresis (PAGE) and transferred by electroblotting onto polyvinylidene difluoride membrane (Millipore). After blocking 1 h with 5% nonfat dry milk ...
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bioRxiv - Microbiology 2020Quote: ... the eluted proteins were loaded to 12% SDS-PAGE gel and then transferred to PVDF membranes (Millipore, Boston, USA). After blocking with 5% non-fat milk for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ... coli total protein extract were spotted within a pre-marked grid onto a 0.2 μm pore–size nitrocellulose membrane (Merk Millipore). The membrane was left to dry to fix the proteins for 30 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant E1E2 was extracted from cell membranes using 1% NP-9 and purified via sequential Fractogel EMD TMAE (Millipore), Fractogel EMD SO3− (Millipore) ...
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bioRxiv - Microbiology 2020Quote: ... supernatant containing solubilized IBs was diluted 1:4 in lysis buffer containing 8M urea and filtered through a 0.22 μm membrane (Millipore). Solubilized IBs were purified by immobilized metal affinity chromatography (IMAC ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was then incubated in blocking buffer for 1 hour at 25°C (TBST with 3% BSA; Sigma) before being probed for LapA or MapA containing an HA tag with an anti-HA antibody (Biolegend ...
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bioRxiv - Cancer Biology 2020Quote: ... Intact and well-shaped slices (approximately 5-10 mm diameter) were then placed on top of a porous membrane insert (0.4 µm, Millipore). Then the membrane inserts were placed into 6-well plates containing 1.5 mL maintenance medium consisting of F12/DMEM (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... membranes were incubated with 5% skimmed milk in PBS supplemented with 0.05% (v/v) Tween 20 (Sigma, PBS-T) for 30 minutes at room temperature (RT) ...
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bioRxiv - Immunology 2021Quote: ... the eluted proteins were loaded onto a 12% SDS-PAGE gel and then transferred to a PVDF membrane (Millipore). After blocking with 5% nonfat milk for 1 h ...
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miR-206 inhibits estrogen-induced proliferation and invasion of ER-α36 positive gastric cancer cellsbioRxiv - Cancer Biology 2021Quote: ... The PVDF membrane was sealed with 5% skim milk powder in the PBS containing tween (Sigma-Aldrich; Merck KGaA) for 3 h ...
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bioRxiv - Cell Biology 2021Quote: ... Membrane were then blocked for 1 hr with 5% milk in Tris-buffered saline plus 0.02% Tween20 (Sigma-Aldrich). Membranes were incubated with the primary antibodies O/N at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... Eluted fractions corresponding to the full ternary complex were gathered and spun concentrated to 14.5 mg/mL using an Amicon Ultrafree 10K NMWL Membrane Concentrator (Millipore). Crystals were grown 1-3 µL hanging drops by varying the ratio of protein to mother liquor from 0.5-2:0.5-2 respectively ...
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bioRxiv - Biochemistry 2022Quote: The cell media were made with DNase- and RNase-free water and filtered through 0.22-μm filter membranes (Millex GV, Millipore) before use ...
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bioRxiv - Physiology 2022Quote: ... Mitochondria activity (membrane potential, PE channel) was measured using a tetramethylrhodamine ethyl ester (TMRE) fluorescent probe (Sigma-Aldrich, 87917) at 400 nM for 30 min at 37 °C ...
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bioRxiv - Neuroscience 2022Quote: ... for 1h.The membrane was incubated overnight in Pierce SuperBlock Blocking Buffer (Pierce #37353) with 0.1% Tween 20 (Sigma #P7949) and validated primary antibodies ...
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bioRxiv - Physiology 2022Quote: ... About 180 handpicked islets were placed onto a polycarbonate membrane (Nuclepore, Whatman) inside a polypropylene perifusion chamber (Swinnex, Millipore) and the oxygenated perifusion solution (modified Krebs-Ringer buffer as above ...
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bioRxiv - Plant Biology 2022Quote: ... The pellets were washed with cytosol extraction buffer for three times and resuspended in membrane extraction buffer (50 mM Tris-Cl pH 7.5, 150 mM NaCl, 2 mM DTT, protease inhibitor cocktail (Sigma), 0.5% NP40 ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were separated by SDS-PAGE with 8% polyacrylamide gel and transferred to PVDF membrane (Millipore, Billerica, MA, USA). Membranes were blocked with 5% skim milk in Tris-buffered saline (20 mM Tris [pH8.0] ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were cultured overnight prior to collecting conditioned media that was filtered through a 0.22-μm pore membrane (Millipore) and treated with 1 mM sodium orthovanadate and Roche Complete Protease Inhibitor Cocktail tablets (according to the manufacturer’s instructions) ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by wet transfer (stacking and resolving gel) in transfer supplemented with 20% methanol on 0.45mm PVDF membrane (IMMOBILON-P, Millipore). PVDF was selected over nitrocellulose as it shows an improved transfer of free SUMO (Castoralova et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... Wet transfers were carried out onto PVDF membranes, blocked in 5% milk in TBS-T (TBS, 0.1% Tween-20 (Sigma)) and incubated with antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... for 45 s for nuclear staining and blocked in 5% bovine serum albumin (BSA, Biowest) for membrane staining or 5% BSA-0.3% Triton-X (Sigma) for nuclear staining ...
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bioRxiv - Molecular Biology 2022Quote: ... the membrane was washed once with TBST and incubated with antibodies against FLAG (1:5000, Sigma, Cat no.# F3165), V5 (1:5000 ...
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bioRxiv - Bioengineering 2022Quote: ... Viral supernatant was harvested at 48 and 72 h post-transfection and filtered through a 0.45 μm PVDF membrane (Merck Millipore). For lentiviral infection ...
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bioRxiv - Cell Biology 2022Quote: ... Western blot signal was detected using BioRad ChemiDoc System upon soaking membranes in Luminata Crescendo HRP substrate (Millipore Sigma), ensuring that the signal was not saturated at any pixel.
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bioRxiv - Cancer Biology 2022Quote: ... Then the membranes were washed with TBST and incubated with Immobilon® Western Chemiluminescent HRP Substrate (EMD Millipore Corporation) or ImmunoStar® LD (Wako ...
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Hypoxia-mediated suppression of pyruvate carboxylase drives tumor microenvironment immunosuppressionbioRxiv - Cancer Biology 2022Quote: ... the membranes were incubated overnight at 4°C with anti-PC antibody (1:500, HPA043922, Millipore-Sigma, Darmstadt, Germany), anti-beta tubulin antibody (1:2000 ...
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Hypoxia-mediated suppression of pyruvate carboxylase drives tumor microenvironment immunosuppressionbioRxiv - Cancer Biology 2022Quote: ... the membranes were incubated overnight at 4°C with anti-PC antibody (1:500, HPA043922, Millipore-Sigma, Darmstadt, Germany), anti-beta tubulin antibody (1:2000 ...
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bioRxiv - Cancer Biology 2022Quote: ... membranes were incubated with primary antibodies diluted in 4% bovine serum albumin (BSA) (Sigma-Aldrich, St. Louis, MO, USA) in TBS-T overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... 20 μg total protein/samples were loaded in each lane of each gel on 7.5-10% SDS-polyacrylamide gel and then transferred to nitrocellulose membrane (Immobilon-P, Millipore). After blocking ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were centrifuged at 12,000 x g for 10 min at 4°C and resultant supernatants were collected and were filtered through a 0.2 mm PTFE (polytetrafluoroethylene) membrane (Millipore). Abscisic acid (ABA)-d6 (gift from Dr ...
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bioRxiv - Microbiology 2020Quote: ... Protein extracts were separated by SDS-polyacrylamide gel electrophoresis then transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, Temecula, CA). Primary antibodies and secondary antibodies were used according to manufacturers’ instructions ...
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bioRxiv - Immunology 2020Quote: ... The separated proteins were transferred to PVDF membranes and blocked in PBS containing 0.2% fish skin gelatin (Sigma-Aldrich) and 0.1% Tween-20 ...
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bioRxiv - Developmental Biology 2020Quote: ... membrane was incubated for 1 h at room temperature with anti-rabbit IgG horseradish peroxidase (HRP) conjugate (Sigma-Aldrich) at a concentration of 1:1000 in 5% (w/v ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were centrifuged at 12,000 x g for 10 min at 4°C and resultant supernatants were collected and were filtered through a 0.2 mm PTFE (polytetrafluoroethylene) membrane (Millipore).
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bioRxiv - Microbiology 2020Quote: ... Ten micrograms of the total lysate was separated in 10% SDS-polyacrylamide gels and transferred to PVDF membranes (Millipore). Primary antibodies used in this paper are mouse anti-CMV IE1/2 monoclonal antibody (MAB8131 ...
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bioRxiv - Cell Biology 2021Quote: ... Denaturated and fully-reduced proteins were resolved on Tris-glycine SDS-PAGE followed by western blot analysis onto PVDF membrane (Immobilon-P, 0.45 μM pore size, Merck Millipore) using enhanced chemiluminescence to detect bound antibodies (Pierce) ...
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bioRxiv - Molecular Biology 2020Quote: ... Fractions containing gp66 were pooled and concentrated (Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-30 membrane, EMD Millipore) to a final concentration 4 mg/ml ...
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bioRxiv - Molecular Biology 2020Quote: ... equal amounts of total protein were separated by 10% SDS-PAGE and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore). The membranes were blocked and incubated with primary antibodies against FGF2 (36769 ...
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bioRxiv - Neuroscience 2021Quote: ... The slices were incubated in this solution for 30 minutes and then placed on a Millicell-CM membrane (Millipore) in culture medium (50% Minimum Essential Medium ...