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Citations for Millipore Sigma :
6401 - 6450 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... the membrane was incubated at 4°C o/n with the monoclonal anti-flag M2 primary antibody (Sigma Aldrich) in TBS-T buffer containing 5% (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE; 10 – 15% acrylamide) and transferred onto PVDF membrane (Millipore). For CD63 western blots ...
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bioRxiv - Cell Biology 2020Quote: Proteins were separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride membranes (Millipore). After in-cubated with antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... the pattern of interest was stamped first on thin Polyvinyl Alcohol membrane made up of 5% PVA solution (Sigma) and transferred the inverted membrane to the substrate ...
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bioRxiv - Developmental Biology 2021Quote: ... The membranes were incubated overnight at 4°C under agitation with the primary antibody anti-H3K9ac (Sigma, cat # H9286) or anti-H3K9bhb (PTM Biolabs cat # PTM-1250 ...
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bioRxiv - Immunology 2021Quote: ... Membranes were washed three times for 5 min in PBST before being incubated with DAPI (50 nM; Sigma-Aldrich) as a nuclear counterstain ...
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bioRxiv - Microbiology 2020Quote: ... 30 μg of total protein was analyzed by SDS-PAGE gel electrophoresis and transferred to a PVDF membrane (Millipore). The membrane was probed with antibodies at 4 °C overnight and visualized using Western Lightning™ Plus ECL (erkinElmer ...
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bioRxiv - Developmental Biology 2021Quote: ... 20 ug to 200 ug of protein was run on a 7.5 or 10% SDS-PAGE gel and transferred onto a PVDF membrane (Sigma-Millipore). The membranes were blotted for 1 hr in 1 ...
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bioRxiv - Cancer Biology 2022Quote: ... and 30µg to 50µg of total proteins were loaded onto 7.5% or 12% SDS-polyacrylamide gels for electrophoresis and transferred onto polyvinylidene difluoride membranes (Millipore). After 1h of blocking with 5% bovine serum albumin or non-fat milk prepared in Phosphate-Buffered Saline (PBS)-0.1% Tween-20 buffer ...
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bioRxiv - Physiology 2022Quote: ... Membranes were then blocked in 5 % skim-milk in 1X Tris-Buffered-Saline-Tween 20 (TBS-T, Sigma-Aldrich) for 60 min ...
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bioRxiv - Biochemistry 2022Quote: ... transferred to nitrocellulose membranes and analyzed using appropriate primary (anti-TAP-Thermo scientific #CAB 1001, anti-GFP-Sigma # 11814460001) and secondary antibodies (HRP goat anti-Mousse-Dako #P0447 ...
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bioRxiv - Neuroscience 2021Quote: ... Tracerline) and filter-sterilized by passage through a Mixed Cellulose Esters membrane (Millex-GS 0.22 μm; SLGS033SB; EMD Millipore). The dyed silicone fluid allowed visualization of the meniscus to confirm the injected volume based on the length of travel of dye along the tubing.
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bioRxiv - Neuroscience 2021Quote: ... Tissue was cut into 400 μM thick sections on a tissue chopper (McIlwain) and cultured on membranes (Millipore PICMORG50) at 37° C in 5% CO2 ...
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bioRxiv - Neuroscience 2021Quote: ... to the top of the cortical layers of brain slice placed on porous (0.4 μm) membrane (Millicell-CM; Millipore) for covering whole slice to maximize the infection rate ...
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bioRxiv - Neuroscience 2021Quote: ... 1 µg of each fraction was loaded in 10% SDS-PAGE gel and transferred to PVDF membrane (Millipore Sigma). Membranes were incubated in blocking solution for 1 h at RT (3% BSA ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were blocked with 5% skim milk and probed with primary antibodies: anti-FLAG (1:1000) (Sigma-Aldrich, F1804), anti-MRG-1 (1:1000 ...
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bioRxiv - Molecular Biology 2020Quote: Proteins were separated by 8% or 10% Tris-Glycine-SDS page gels for 1 h and transferred on PVDF membrane (0.45 um, Millipore). Then blocked the membrane with 5% skimmed milk in TBS-T buffer (20 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2022Quote: ... 1×104 epithelial cells in 25 μL media and 2×104 mesenchymal cells in 25 μL media per insert (12 mm cell culture inserts with 0.4 µm membrane Millipore) were prepared ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were washed with TBS-Tween (0.01% v/v) prior to detection using enhanced chemiluminescent (ECL) reagent (Millipore) and imaged using a ChemiDoc Imaging System (BioRad) ...
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bioRxiv - Immunology 2021Quote: ... Protein lysates (25 μg) were resolved by SDS-PAGE and transferred to Immobilon-P PVDF membrane (Sigma-Aldrich, IPVH00010). Membranes were blocked in 5% nonfat milk in TBST buffer at room temperature for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... were resuspended and treated by Laemmli buffer and then were separated in 10% SDS-PAGE and electroblotted onto 0.45 µm polyvinylidene difluoride membranes (PVDF; Millipore) using Trans-blot Turbo (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were separated on an SDS-PAGE gel and then transferred to a nitrocellulose (NC) membrane (Merck Millipore, ISEQ00010), which was incubated with individual protein-specific primary antibodies at 4 °C overnight on a shaker ...
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bioRxiv - Developmental Biology 2020Quote: ... Either 10 or 20 ul of sample were separated by 12 % SDS-PAGE and transferred onto PVDF membrane (Millipore). After blocking with 5 % Milk powder (Roth ...
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bioRxiv - Systems Biology 2020Quote: ... Cultures were grown to mid-log and 1 mL was transferred to a 0.45 μm PVDF membrane filter paper (Millipore), fixed atop a vacuum manifold ...
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bioRxiv - Cell Biology 2020Quote: ... Viability of cells after exposure to Dharmafect reagent and specific RNAi’s was confirmed by the lack of membrane damage detected by staining with propidium iodide (Sigma) and flow cytometry ...
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bioRxiv - Physiology 2020Quote: ... Proteins were transferred onto nitrocellulose membranes and immunoblot analyses were carried out using rabbit antibodies directed against ShcA (Millipore), Collagen X (Abcam) ...
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bioRxiv - Physiology 2020Quote: ... Proteins were resolved on a 4%–12% SDS-PAGE gel and transferred to a PVDF membrane (Immobilon-FL, Millipore), incubated with indicated antibodies overnight at 4°C and subsequently quantitated with the LiCOR Odyssey instrument with Odyssey Image Studio software using 2 different infrared channels ...
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bioRxiv - Biochemistry 2021Quote: ... The trace elements stock solution was sterilized by filtration through a 0.22 µm polyether sulfone membrane (Merck-Millipore, Germany). Note that insoluble elements present in this solution were removed by the filtration step ...
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bioRxiv - Plant Biology 2021Quote: ... The membranes were probed for 16 h with Concanavalin A conjugated with horseradish peroxidase (1 mg/mL; Sigma-Aldrich), washed for 45 min with PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... Proteins were separated on 4-15% acrylamide gels and transferred for 60 minutes at 12.0 V to PVDF membranes (Millipore). Membranes were blocked in 5% BSA in TBS (20 mM Tris pH 7.6 ...
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bioRxiv - Immunology 2021Quote: ... 4 million cells per condition were washed once with ice-cold saline solution (0.9% NaCl solution filtered through a 0.22 μm membrane filter) and resuspended in 1 ml of 50% (vol/vol) methanol (Sigma) (0.22 μm-filtered ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were incubated with peroxidase-coupled secondary IgG antibodies (anti-Rabbit–A0545 or anti-Mouse –A9044, Sigma Aldrich) for 2 h at room temperature ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Membranes were blocked with Odyssey blocking buffer in PBS (Li-COR) and incubated with anti-FLAG M2 (F1804; Sigma) and anti-c-Myc (C3956 ...
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bioRxiv - Biophysics 2021Quote: The spatial distribution of mitochondrial membrane potential within oocytes was measured with a potential-sensitive dye TMRM (Sigma Aldrich). Oocytes were cultured in AKSOM with 100 nM TMRM for 30 minutes before imaging ...
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bioRxiv - Neuroscience 2021Quote: ... recordings were obtained for retinal fragments (without the retinal pigment epithelium attached) placed on a cellulose membrane that had been incubated with polylysine (0.1%, Sigma) overnight ...
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bioRxiv - Biophysics 2021Quote: ... The cells were placed on a filter (25 mm, 0.4 µm, Nuclepore Track-Etch Membrane, Whatman) and dehydrated with 2,2-dimethoxipropane (Sigma-Aldrich). Subsequently ...
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bioRxiv - Bioengineering 2021Quote: ... all solutions were purified using Amicon centrifugal ultra-filtration devices (Amicon Ultra-2, Sigma Aldrich, 100 kDa membrane, Merck). Prior to use ...
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bioRxiv - Biochemistry 2020Quote: ... Solubilized proteins were separated from the insoluble fraction by centrifugation at 60,000 g for 1 hr at 4° C and the supernatant was filtered through a 0.22-μm polystyrene membrane (Millipore). GFP nanobody was coupled to CNBr-activated Sepharose Fast Flow beads (GE healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... Solubilized proteins were separated from the insoluble fraction by centrifugation at 60,000 x g at 4° C for 1 hr and the supernatant was filtered through a 0.22-μm polystyrene membrane (Millipore). 2 mL GFP nanobody resin was incubated with the sample with agitation at 4 °C for 1 hr ...
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bioRxiv - Biochemistry 2020Quote: Proteins separated on PAGE gel were transferred to a PVDF membrane (Immobilon-P/Immobilon-FL, 0.45 μm, Merck Millipore) using Trans-Blot® Turbo™ Transfer System (Bio-Rad) ...
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bioRxiv - Biochemistry 2020Quote: ... Solubilized total proteins were separated by SDS-PAGE and electro-blotted onto a PVDF membrane (Merck Millipore; Billerica, MA). The membrane was first blocked with 5% skim milk in PBST (Phosphate Buffered Saline with Tween 20) ...
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bioRxiv - Neuroscience 2021Quote: ... The purified viral particles were then concentrated using an ultrafiltration membrane unit (Amicon Ultra; EMD Millipore, Burlington, MA, USA). Virus titers were determined by quantitative PCR using a pair of primers for the WPRE sequence (forward ...
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bioRxiv - Neuroscience 2021Quote: ... for 1h at RT, membranes were developed with ECL substrate (10% Tris HCl 1M pH 8.5, 0.5% coumaric acid (Sigma-Aldrich), 0.5% luminol (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... The protein complexes associated with p53 were resolved by SDS-PAGE and transferred onto a PVDF membrane (#IPVH00010, Millipore). The membrane was blocked with 3% BSA in PBS for 1 h at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... membranes are blocked with 5% milk or BSA for phosphorylated proteins in TBS+0.2% Tween®20 (Sigma-Aldrich) for 1h and incubated 1h with primary antibody at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... and were transferred at 100 mA for 70 min onto PVDF membrane (Immobilon-P, 0.45 μM pore size, Merck Millipore) using semi-dry blotting system ...
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bioRxiv - Neuroscience 2021Quote: ... samples were heated 5 minutes at 95°C and 30µl were separated by SDS-PAGE and transferred onto PVDF membranes (Immobilion-P, Merck Millipore) according to standard protocols ...
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bioRxiv - Molecular Biology 2020Quote: ... transferred to nitrocellulose membrane and probed with primary antibodies against HA (HA-7 mouse monoclonal, 1:2000 dilution, Sigma; or rabbit polyclonal ...
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bioRxiv - Plant Biology 2021Quote: ... The samples were resolved by SDS-PAGE and transferred to nitrocellulose membrane for immunoblotting analysis with anti-GFP (Sigma), −BIP (enzolifesciences) ...
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bioRxiv - Biochemistry 2021Quote: ... The membranes were incubated for 1 h at room temperature with horseradish peroxidase-conjugated goat anti-rabbit IgG (Sigma) at a 1:10,000 dilution ...