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Citations for Millipore Sigma :
6201 - 6250 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2023Quote: ... HUVECs were then exposed to shear stress for 24 hours with or without Tubastatin-A (3 µM; Sigma-SML0044). For experiments where autophagic flux was blocked ...
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bioRxiv - Bioengineering 2023Quote: ... the organosilane used was hexadecyltriethoxysilane (HDTES, Gelest Inc, cat no SIH5922.0) rather than (3-aminopropyl) trimethoxy silane (APTMS, Sigma).
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bioRxiv - Bioengineering 2023Quote: ... with the substitution of hexadecyltriethoxysilane (HDTES, Gelest Inc., cat no SIH5922.0) as the organosilane rather than (3-aminopropyl) trimethoxy silane (APTMS, Sigma).
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bioRxiv - Bioengineering 2022Quote: ... 3 mM aniline dimer solution was prepared by dissolving 5.5 mg of N-phenyl-p-phenylenediamine (Sigma-Aldrich, 241393) in 10 mL of Tyrode’s solution ~20 h at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: Cells were collected by trypsinisation and resuspended in Phosphate-buffered saline and incubated with 200μM cyanide 3-chlorophenylhydrazone (CCCP, Sigma) for 15 min as TMRE negative control ...
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bioRxiv - Immunology 2023Quote: ... juvenile or adult zebrafish were added to water containing 0.08 mg/mL Tricaine (MS222/ethyl 3-aminobenzoate; Sigma-Aldrich) until fish became non-responsive to touch (<5 minutes) ...
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bioRxiv - Cell Biology 2023Quote: ... Sections of 3 μm in the thickness were conducted for Safranin O and fast green and TRAP (Sigma-Aldrich) staining ...
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bioRxiv - Biophysics 2023Quote: ... Unbound dye was removed by sequential buffer exchange in centrifugal filters Amicon Ultra 0.5 ml 3 K (Merk Millipore). Lge11-80-Dylight labelled protein was stored at -80 °C.
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bioRxiv - Neuroscience 2023Quote: ... USA). 3- sulfopropionic acid (cat. #BD01044758) was purchased from BLD pharma. Tramiprosate (cat. # A76109) was purchased from Sigma Aldrich. GM1 and GM3 ...
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bioRxiv - Neuroscience 2023Quote: ... The cell viability was measured by quantifying the activity of cellular dehydrogenases that reduce MTT (3-4,5-dimethyl thiazolyl-2,5-diphenyl-2H-tetrazolium bromide, Sigma Inc.) to a purple formazan salt [35] ...
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bioRxiv - Neuroscience 2023Quote: 17β-Estradiol (E2, E8875), β-Estradiol-3-Benzoate (EB, E8515) and Progesterone (P, P0130) were purchased from Sigma-Aldrich and dissolved in sesame oil ...
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bioRxiv - Evolutionary Biology 2023Quote: ... medaka and zebrafish were deeply anesthetized with 0.02% ethyl 3-aminobenzoate methanesulfonate (MS-222) (Sigma-Aldrich, St. Louis, MO) and fixed by perfusion with 4% paraformaldehyde (PFA ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was transferred to an Amicon Ultra-0.5 Centrifugal Filter Unit 3 kDa (Merck Millipore, Catalogue no. UFC500396) and centrifuged at 10,000 g for 20 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were again washed 3 × 5 min with PBS + 0.1% Tween 20 and incubated with Classico/Crescendo Western HRP substrate (Millipore-Sigma) for 2 min ...
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bioRxiv - Microbiology 2023Quote: ... These lysates were then additionally treated for protein precipitation with 3 volumes of ultra-pure methanol (Sigma-Aldrich, USA). Ultimately ...
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bioRxiv - Plant Biology 2023Quote: ... spy-3 and spy-4 were sprayed with either 50 µM or 100 µM BA (6-benzylaminoadenine, Sigma B3408) or mock [200 mM NaOH] three times ...
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bioRxiv - Microbiology 2023Quote: ... For the serial passaging MDCK cell were infected at a multiplicity of infection (MOI) of 0.0001 and incubated for 3 days at 37°C in DMEM containing TPCK-Trypsin (Sigma) at a final concentration of 1 µg/mL ...
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bioRxiv - Genetics 2023Quote: ... or a shRNA specific for human SOX11 (100% identity to the equine SOX11 sequence) (5’-CTGGTGGATAAGGATTTGGAT-3’ clone NM_003108.3-1235s1c1; Sigma-Aldrich) were created by transfection into HEK293T packaging cells ...
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bioRxiv - Cell Biology 2024Quote: ... The small interfering siRNA (siRNA) oligonucleotide targeting human FCHSD2 (5’-GCAUACUCCUGAGACCUCA[dT][dT]-3’) was obtained from Sigma Aldrich. FCHSD2 siRNA knockdown in all HeLa cell lines was performed for 48 h using the DharmaFECT transfection reagent (Dharmacon ...
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: ... and then blocked for 3 hr at room temperature or overnight at 4 °C in BSA (Sigma-Aldrich A2153) 3% in PBS-T ...
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bioRxiv - Developmental Biology 2024Quote: ... they were first anesthetized using egg water containing 0.016% tricaine (3-amino benzoic acid ethyl ester) from Sigma-Aldrich. Subsequently ...
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bioRxiv - Developmental Biology 2024Quote: ... 50-60 µm cryosections were blocked for 3h at room temperature in PBS containing 3% BSA (A7906, Sigma-Aldrich) and 0.5%Triton X-100 (T9284 ...
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bioRxiv - Cell Biology 2024Quote: ... The chips were then washed with PBS 3 times before incubation with 0.5% (v/v) glutaraldehyde (Sigma-Aldrich, G6257) in PBS at RT for 10-15 min ...
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bioRxiv - Biophysics 2024Quote: ... Excised tissues are first allowed to fold into spheroids and seal in HM for 3-6 hours and are then embedded in 0.5% low gelling point agarose (Sigma) prepared in HM to reduce tissue movement during imaging ...
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bioRxiv - Systems Biology 2024Quote: ... UK) previously coated with calf- skin collagen (15 µg/cm2 and fibronectin 3 µg/cm2; both Sigma-Aldrich, UK), were transfected with one of three different commercial siRNA sequences designed to target the EGFR or an Allstars negative control siRNA sequence (final concentration 1 nM ...
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bioRxiv - Systems Biology 2024Quote: ... UK) previously coated with calf- skin collagen (15 µg/cm2 and fibronectin 3 µg/cm2; both Sigma-Aldrich, UK). The permeability of hCMEC/D3 cell monolayers to 70 kDa FITC-dextran (2 mg/ml ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were treated with RNase A (345 μL TE, 3 μL 10 mg/mL RNAse A (Sigma-Aldrich, R6513), 2 μL 20 mg/mL Glycogen (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... paraformaldehyde in PBS and incubated for 30 min in blocking buffer containing 0.3% Triton X100 and 3% BSA (Sigma) in PBS to permeabilize the cells and block nonspecific staining ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were rinsed with DPBS 3 times and dissociated with 0.5 mM EDTA (Merck Millipore, Burlington, MA, USA, #324503) for 8-10 min at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... the marine nanofibers were washed three times with 500 μL of ultrapure water using an Amicon Ultra-0.5 Centrifugal Filter Unit (3 kDa cutoff) (Merck Millipore). The collected marine nanofibers were dropped onto a copper grid ...
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bioRxiv - Neuroscience 2024Quote: All female voles were ovariectomized and primed through subcutaneous administration of estradiol benzoate (17-β-Estradiol-3-Benzoate, Sigma, 2 μg dissolved in sesame oil starting 3 days prior to the experiments ...
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bioRxiv - Microbiology 2024Quote: ... the cells underwent 3 freeze/thaw cycles followed by treatment with 0.05U/ml neuraminidase type V (Sigma-Aldrich; N2876) at 37°C for 16 hours ...
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bioRxiv - Microbiology 2024Quote: ... Cells were washed two more times with PBS then blocked and permeabilised using a 3% BSA (Sigma-Aldrich, 1073508600), 0.2% Triton X-100 (VWR ...
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bioRxiv - Microbiology 2024Quote: ... supernatants were concentrated down to 3 ml using Amicon® Ultra-15 Centrifugal Filter Unit (50 kDa cutoff, Millipore) centrifuging at 3,500 x g for 10 min at 4°C each time ...
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bioRxiv - Microbiology 2024Quote: ... Overlay was prepared by mixing equal volumes of 3% (w/v) low-temperature gelling agarose (Sigma-Aldrich, Ref. A4018) with undiluted overnight culture ...
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bioRxiv - Genomics 2024Quote: ... was added to the bead slurry and the entire solution was pipetted as a large droplet onto a 25.4 mm × 76.2 mm microscope glass slide functionalized with 3-(Trimethoxysilyl)propyl methacrylate (Sigma-Aldrich) as described previously (19) ...
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bioRxiv - Immunology 2024Quote: ... Tumor tissue was chopped into small pieces in 3-5mL of digestion buffer: 1mg/mL collagenase (Sigma-Adrich C0130), 20 µg/mL DNase I (STEMCELL Technologies 7469) ...
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Angiogenesis in the mature mouse cortex is governed in a region specific and Notch1 dependent mannerbioRxiv - Neuroscience 2024Quote: ... mice were intravenously injected with FITC-dextran (100µL of 3-5% w/v in 0.9% saline; 70kDa, Sigma #46945) or Texas Red dextran (100µL of 3% w/v in 0.9% saline ...
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bioRxiv - Neuroscience 2024Quote: Stock solutions (1mg/ml) of 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) in N,N-dimethylformamide (Sigma-Aldrich 468495) were stored at −20°C ...
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bioRxiv - Physiology 2024Quote: ... The PDMS scaffold surface was activated oxygen plasma and then treated with a 5% (3-aminopropyl) triethoxysilane (Sigma-Aldrich) solution in methanol for 45 min ...
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bioRxiv - Neuroscience 2024Quote: ... Free-floating organotypic slices or vibratome sections were incubated in permeabilization solution (0.2% Triton-X100, 3% BSA in PBS; Sigma) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... All the ASO probes were synthesized with biotin-TEG added to the 3’ end of the sequences (Sigma-Aldrich). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... washed with PBS (3 times x 5 minutes) and incubated with secondary antibodies and Hoescht 33342 (Sigma-Aldrich, #14533) diluted in 1x Antibody diluent/Block solution for 2 hours at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... the culture medium was changed to medium supplemented with 500 μM 3-Indoleacetic acid (IAA; Sigma-Aldrich, I2886-5G) and 1 μg/mL doxycycline (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were seeded at 1.0E5 cells/ml with 10 ng/ml IL-3 and 8μg/ml polybrene (Sigma-Aldrich). Virus was added to wells at 0 ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were then washed in PBS and incubated in 0.2% Sudan black in 70% ethanol at room temperature for 3 minutes to minimize autofluorescence and mounted on glass slides (Menzel-Gläser) with FluorSave (Merck Millipore).
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bioRxiv - Neuroscience 2024Quote: ... cells were washed three times in PHEM buffer and permeabilized for 3 minutes in 0.02% Triton-X 100 (Sigma). Cells were then blocked in 5% BSA (Sigma ...
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bioRxiv - Physiology 2024Quote: ... 30 μL of XTT dye (stock 0.1 mg/µL in DMSO) and 7.5 μL phenazine methosulfate (stock 3 mg/mL in PBS) (Millipore Sigma) were added to 3 mL of phenol-free DMEM ...
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bioRxiv - Cancer Biology 2024Quote: ... The fixed and permeabilized cells were then exposed to a blocking solution (3% bovine serum albumin (#A7906, Sigma‒Aldrich) in PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... was buffered to a pH of 7.0 using 0.165 mol/L 3-(N-morpholino)propanesulfonic acid (MOPS; Sigma, USA) and supplemented with 2% glucose ...