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Citations for Millipore Sigma :
6151 - 6200 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The harvested cells were resuspended in immobilised metal chromatography (IMAC) buffer (50 mM Tris-HCl [pH 7.5], 300 mM NaCl) supplemented with 3 kU/ml rLysozyme (Novagen). The cell suspension was sonicated on ice (Ultrasonic disruptor UD-201 ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were anaesthetised in a solution of buffered ethyl 3-aminobenzoate methanesulfonate salt (100 mg/L; MS-222, Sigma) in seawater ...
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bioRxiv - Immunology 2022Quote: ... 7.5×107/15ml RPMI+3%FBS and stimulated with 25nM phorbol 12-myristate 13-acetate (PMA; Millipore-Sigma #P8139) Plates were incubated at 37°C ...
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bioRxiv - Immunology 2022Quote: ... 7.5×107/15ml RPMI+3%FBS and stimulated with 25nM phorbol 12-myristate 13-acetate (PMA; Millipore-Sigma #P8139) Plates were incubated at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... then fixed with 4% paraformaldehyde for 30 min at room temperature and washed 3 times in PBS.Cells were permeabilized with 0.1% Triton-X100 (Sigma T8787) and washed 3 times in PBS ...
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bioRxiv - Immunology 2023Quote: ... which was precoated for 3 hours at 37°C with 4ug/mL fibrinogen (Sigma-Aldrich; Saint Louis, MO, USA). After letting the cells settle for 3 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-tagged CLAMP proteins were eluted for 3 h at 4°C on a rotating wheel in the presence of 0.5 mg/ml FLAG-Peptide (Sigma) in 1 ml Buffer C containing 1x PIC ...
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bioRxiv - Neuroscience 2022Quote: ... The dopamine- synthesis inhibitor 3-Iodo-L-tyrosine (3IY; CAS: 70-78-0, Sigma Aldrich; concentration: 5 mg/ml) was added to samples of 2 ml yeast solution ...
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bioRxiv - Microbiology 2022Quote: ... the bottom channel of a polydimethylsiloxane (PDMS) device was precoated with fibronectin (3 μg/ml, Sigma-Aldrich, Cat# F1141). The microfluidic device was generated according to our previous report63 ...
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bioRxiv - Microbiology 2022Quote: ... Calu-3 cells (ATCC, HTB-55) were maintained in Eagle’s minimum essential medium (EMEM) (Sigma-Aldrich, Cat# M4655-500ML) containing 10% FBS and 1% PS ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were permeabilized with 0.01% Triton X-100 in PBS for 15 minutes and blocked with 3% bovine serum albumin (A7906, Sigma) in 0.01% Triton X-100 in PBS (blocking buffer ...
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bioRxiv - Microbiology 2023Quote: ... embryos were mechanically dechorionated and anaesthetized in 0.02 % (w/v) buffered 3-aminobenzoic acid methyl ester (pH 7.0) (Tricaine, Sigma-Aldrich). Zebrafish embryos were individually infected by microinjection with 1 nl of P ...
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bioRxiv - Bioengineering 2022Quote: ... 300 μL of supernatant was filtered under 3 kDa through Amicon Ultra-0.5 mL Centrifugal Filters (Millipore, Burlington, MA) or 96-well plates (Pall Corporation ...
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bioRxiv - Microbiology 2022Quote: ... cultures were pelleted by centrifugation at 1,600 rpm for 3 min and resuspended in a 10× volume of 5% sorbitol (Sigma), followed by incubation at 37°C for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... Calu-3 cells (ATCC, HTB-55) were maintained in Eagle’s minimum essential medium (EMEM) (Sigma-Aldrich, Cat# M4655-500ML) containing 10% FBS and 1% PS ...
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-tagged MSL proteins were eluted for 3 h at 4°C on a rotating wheel in the presence of 0.5 mg/ml FLAG-Peptide (Sigma) in 1 ml Elution buffer ...
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bioRxiv - Neuroscience 2023Quote: ... tissues were washed 3 times with PBS-T (0.2% Triton X-100 in PBS (PBST) with 0.01% sodium azide (Sigma Aldrich)) and blocked for 1 hour in 10 % Goat Serum (Sigma Aldrich) ...
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bioRxiv - Biophysics 2023Quote: ... NMR samples were buffer exchanged into NMR buffer (pH 7.4 or pH 7.8) using Amicon Ultra-15 centrifugal concentrators (3-kDa cutoff, Millipore Sigma) to a final nucleic acid concentration of ∼1 mM ...
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bioRxiv - Neuroscience 2023Quote: ... by dispensing individual cells in 5 nL drops directly into 3 µL ice-cold single cell lysis buffer (scLB, 0.134% Triton X-100 [Sigma] ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then permeabilized with 0.1% Triton-X for 10 min and blocked with 3% goat serum (GS) (Sigma) for 30 mins at room temperature (unless stated otherwise) ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was concentrated and using using Amicon Ultra-15 centrifugal filters (3 kDa cut-off, Merck-Millipore) and then buffer was exchanged to buffer B (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Neuroscience 2023Quote: ... 3-5 organoids per condition were dissociated by incubation with a solution of 0,5 mg/ml Collagenase/Dispase (Sigma) + 0,22 mg/ml EDTA (Euroclone ...
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bioRxiv - Neuroscience 2023Quote: Cells in the tectum were transfected using in vivo electroporation of stage 46 animals anesthetized in 0.01%MS-222 (3-aminobenzoic acid ethyl ester, Sigma) dissolved in Steinberg’s media ...
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bioRxiv - Immunology 2023Quote: ... Tumors were digested for 45 minutes with gentle shaking at 37°C in HBSS containing 3 mg/ml Collagenase IV and 2.8 mg/ml DNase-I (Sigma). Cells from BRAFi (n = 5 ...
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bioRxiv - Immunology 2023Quote: ... and fixed for 3 hr at room temperature (RT) in freshly prepared Carnoy’s fixative [60% anhydrous methanol (Sigma-Aldrich), 30% chloroform (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... wells were incubated with 200 µL of a blocking buffer (PBS - Bovine Serum Albumin 3% (BSA reference: A9418, Sigma)) during one hour at 35°C ...
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bioRxiv - Bioengineering 2023Quote: ... or C43 cells in M9 minimal media supplemented with 0.5 g 15N NH4Cl2 (Cambridge isotopes) and/or 3 g 13C glucose (Sigma) per liter as well as 0.2 g/L of isotope- enriched ISOGROW supplements (Sigma) ...
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bioRxiv - Pathology 2023Quote: ... HUVECs were then exposed to shear stress for 24 hours with or without Tubastatin-A (3 µM; Sigma-SML0044). For experiments where autophagic flux was blocked ...
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bioRxiv - Bioengineering 2023Quote: ... the organosilane used was hexadecyltriethoxysilane (HDTES, Gelest Inc, cat no SIH5922.0) rather than (3-aminopropyl) trimethoxy silane (APTMS, Sigma).
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bioRxiv - Bioengineering 2023Quote: ... with the substitution of hexadecyltriethoxysilane (HDTES, Gelest Inc., cat no SIH5922.0) as the organosilane rather than (3-aminopropyl) trimethoxy silane (APTMS, Sigma).
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bioRxiv - Bioengineering 2022Quote: ... 3 mM aniline dimer solution was prepared by dissolving 5.5 mg of N-phenyl-p-phenylenediamine (Sigma-Aldrich, 241393) in 10 mL of Tyrode’s solution ~20 h at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: Cells were collected by trypsinisation and resuspended in Phosphate-buffered saline and incubated with 200μM cyanide 3-chlorophenylhydrazone (CCCP, Sigma) for 15 min as TMRE negative control ...
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bioRxiv - Immunology 2023Quote: ... juvenile or adult zebrafish were added to water containing 0.08 mg/mL Tricaine (MS222/ethyl 3-aminobenzoate; Sigma-Aldrich) until fish became non-responsive to touch (<5 minutes) ...
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bioRxiv - Cell Biology 2023Quote: ... Sections of 3 μm in the thickness were conducted for Safranin O and fast green and TRAP (Sigma-Aldrich) staining ...
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bioRxiv - Biophysics 2023Quote: ... Unbound dye was removed by sequential buffer exchange in centrifugal filters Amicon Ultra 0.5 ml 3 K (Merk Millipore). Lge11-80-Dylight labelled protein was stored at -80 °C.
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bioRxiv - Neuroscience 2023Quote: ... USA). 3- sulfopropionic acid (cat. #BD01044758) was purchased from BLD pharma. Tramiprosate (cat. # A76109) was purchased from Sigma Aldrich. GM1 and GM3 ...
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bioRxiv - Neuroscience 2023Quote: ... The cell viability was measured by quantifying the activity of cellular dehydrogenases that reduce MTT (3-4,5-dimethyl thiazolyl-2,5-diphenyl-2H-tetrazolium bromide, Sigma Inc.) to a purple formazan salt [35] ...
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bioRxiv - Neuroscience 2023Quote: 17β-Estradiol (E2, E8875), β-Estradiol-3-Benzoate (EB, E8515) and Progesterone (P, P0130) were purchased from Sigma-Aldrich and dissolved in sesame oil ...
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bioRxiv - Evolutionary Biology 2023Quote: ... medaka and zebrafish were deeply anesthetized with 0.02% ethyl 3-aminobenzoate methanesulfonate (MS-222) (Sigma-Aldrich, St. Louis, MO) and fixed by perfusion with 4% paraformaldehyde (PFA ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was transferred to an Amicon Ultra-0.5 Centrifugal Filter Unit 3 kDa (Merck Millipore, Catalogue no. UFC500396) and centrifuged at 10,000 g for 20 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were again washed 3 × 5 min with PBS + 0.1% Tween 20 and incubated with Classico/Crescendo Western HRP substrate (Millipore-Sigma) for 2 min ...
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bioRxiv - Microbiology 2023Quote: ... These lysates were then additionally treated for protein precipitation with 3 volumes of ultra-pure methanol (Sigma-Aldrich, USA). Ultimately ...
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bioRxiv - Plant Biology 2023Quote: ... spy-3 and spy-4 were sprayed with either 50 µM or 100 µM BA (6-benzylaminoadenine, Sigma B3408) or mock [200 mM NaOH] three times ...
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bioRxiv - Microbiology 2023Quote: ... For the serial passaging MDCK cell were infected at a multiplicity of infection (MOI) of 0.0001 and incubated for 3 days at 37°C in DMEM containing TPCK-Trypsin (Sigma) at a final concentration of 1 µg/mL ...
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bioRxiv - Genetics 2023Quote: ... or a shRNA specific for human SOX11 (100% identity to the equine SOX11 sequence) (5’-CTGGTGGATAAGGATTTGGAT-3’ clone NM_003108.3-1235s1c1; Sigma-Aldrich) were created by transfection into HEK293T packaging cells ...
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bioRxiv - Microbiology 2023Quote: ... Calu-3 cells (ATCC, HTB-55) were maintained in Eagle’s minimum essential medium (EMEM) (Sigma-Aldrich, Cat# M4655-500ML) containing 10% FBS and 1% PS ...
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bioRxiv - Bioengineering 2023Quote: ... they were incubated with 4 mg/mL of N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC; Sigma-Aldrich, Schnelldorf, Germany) dissolved in 100 mM 2-(N-morpholino ...
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bioRxiv - Biophysics 2023Quote: ... washed 3×20min with milli-Q water and stained overnight with Coomasie Brillant Blue (G250, Sigma, Saint-Louis, USA). They were finally rinced 3×20 min with milli-Q water.
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bioRxiv - Microbiology 2023Quote: ... Zebrafish larvae were anesthetized with egg water containing 0.02% buffered 3-aminobenzoic acid ethyl ester (Tricaine, Sigma-Aldrich, Netherlands) for bacterial infection and imaging experiments.
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bioRxiv - Microbiology 2023Quote: ... permeabilised with 0.1% Triton X-100 in PBS for 10 minutes and blocked in 3% BSA (Sigma Aldrich, #A9418) for 1 hour at RT ...