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Citations for Millipore Sigma :
6051 - 6100 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: ... Eluted proteins were exchanged into 20 mM sodium phosphate buffer containing 50 mM NaCl (pH 7.4) and concentrated using Amicon Ultra-15 centrifugal filters with a molecular weight cut-off of 3 kDa (Millipore) for STEPtag ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 isolate nCoV-WA1-2020 (MN985325.1)18 (Vero passage 3) was kindly provided by CDC and propagated once in VeroE6 cells in DMEM (Sigma) supplemented with 2% fetal bovine serum (Gibco) ...
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bioRxiv - Microbiology 2021Quote: ... and the resultant filtrates were concentrated using Amicon Ultra Centrifugal Filter Units with a 3 kDa cutoff (EMD Millipore). Protein concentration was quantified using the Bio-Rad protein assay (Bio-Rad Laboratories) ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted fraction containing purified bacteriocin was concentrated to 4.6 mg/mL in an Amicon centrifugal filter concentrator with a 3 kDa cutoff membrane (Millipore) in buffer comprising 20 mM sodium phosphate (pH 6 ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were washed 3 times with 0.1 M phosphate buffer and incubated with filtered thiocarbohydrazide (TCH, Sigma, 223220) for 45 mins at 40 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Electrodes were coated with 1,1′-dioctadecyl-3,3,3′,3′-tetramethyl-indocarbocyanine perchlorate fluorescent dye (DiI, Sigma; (DiCarlo et al., 1996)) ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were washed in PBST 3 times for 10 minutes and mounted using MOWIOL-DABCO Mounting Media (Sigma, #10891) and then dried at room temperature overnight in a dark box ...
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bioRxiv - Immunology 2021Quote: ... and two pools of S2 peptides (pools 3 and 4) (final concentration of 2μg/mL) on IPVH-membrane plates (Millipore) coated with 5μg/mL anti-mouse IFNγ (AN18) ...
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bioRxiv - Cancer Biology 2020Quote: ... the cells were cultured for 3 days in the presence of 3μg/ml Blasticidin S (Sigma, stock 2.5mg/ml) for selection and then serially diluted to generate single cell clones ...
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bioRxiv - Cancer Biology 2020Quote: CM (without BSA) were concentrated using 3 kDa molecular weight cut-off (MWCO) Amicon ultracentrifugal units (Millipore, Burlington, MA) and lyophilized overnight ...
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bioRxiv - Biochemistry 2020Quote: ... Blots were washed 3 times for 5 min and incubated with anti-mouse-HRP antibody (0.2 μg/ml – Millipore) for 1 h in TBST ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Samples were derivatized in RT for 3 min with 10 μl of 60% p-toluenesulfonyl isocyanate (PTSI) (Sigma Aldrich) in ACN (Hu et al. ...
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bioRxiv - Plant Biology 2020Quote: ... The bottom of the tilted plates was filled with 13 ml of 0.5 M glucose solution for glucose supplementation experiments or 100 μM 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU, Sigma®) for sugar starvation experiments ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Cre reverse primer 5’-GGT TTC CCG CAG AAC CTG AA-3’ (both primers were purchased from Sigma-Aldrich).
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bioRxiv - Microbiology 2021Quote: ... The strain was cultured for 6 hours in BHI with 0.2% Tween 80 and washed 3 times with M9 minimal medium (11.28 g/L M9 salts (Sigma-Aldrich), 0.1 mM CaCl2 ...
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bioRxiv - Developmental Biology 2021Quote: Embryos for imaging were anesthetized with egg water containing 0.016% tricaine (3-amino benzoic acid ethyl ester, Sigma-Aldrich), and then embedded in 1% low melting point agarose (Invitrogen 16520050) ...
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bioRxiv - Biochemistry 2021Quote: ... adjusted to pH 5.8 using 3 M HCl and filtered with 0.2 μm nylon membrane filters (GNWP04700, 0.2 μm pore size, Merck Millipore Ltd.), while mobile phase B consisted of 100% methanol ...
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bioRxiv - Biochemistry 2021Quote: ... adjusted to pH 8 using 3 M HCl and filtered with 0.2 μm nylon membrane filters (GNWP04700, 0.2 μm pore size, Merck Millipore Ltd.), while mobile phase B consisted of 100% methanol (method described in Table 2) ...
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bioRxiv - Biochemistry 2021Quote: ... 3-trimethylsilyl propionic acid (TSP) and 2,2,2-trifluoroethanol (TFE-d3) of analytical grade were obtained from Sigma-Aldrich (Ireland).
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bioRxiv - Biochemistry 2021Quote: ... Elution fractions containing the protein of interest were concentrated using a centrifugal filter (Amicon Ultra, MWCO 3 kDa, Sigma) before loading onto a size-exclusion Sepharose 200 column (Cytiva ...
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bioRxiv - Developmental Biology 2021Quote: ... the media was replaced with 5mL of digestive enzyme mixture (3 mg/mL collagenase I (Cat.No. C0130; Sigma-Aldrich), 3 mg/mL collagenase II (Cat.No ...
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bioRxiv - Plant Biology 2021Quote: ... N-hexanoyl-L-homoserine lactone (C6-HSL) and N-3-oxotetradecanoyl-L-homoserine lactone (oxo-C14-HSL) (Sigma-Aldrich) at concentrations as indicated were used in this study.
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bioRxiv - Pathology 2021Quote: ... the lyophilized antibody panel was suspended in antibody diluent buffer (TBS-IHC tween, Donkey serum 3%) and filtered with a 0.1 µm filter (Millipore).
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bioRxiv - Developmental Biology 2020Quote: ... were post-fixed with 4% para-formaldehyde and blocked with PBS solution containing 0.3% Triton-X 100 and 3% normal Donkey serum (Sigma). Antibody dilution buffer consisted of PBS containing 1% BSA and 0.3% Triton-X 100 ...
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bioRxiv - Cancer Biology 2021Quote: ... The 24h-conditioned medium was then concentrated to 0.2 mg/ml through Amicon filters (3 kDa cut-off, Millipore), and incubated in cleavage buffer with or without pepstatin A (12.5 µM ...
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bioRxiv - Biophysics 2020Quote: ... the polymerization was catalysed by adding 3 % (v/v) N,N,N’,N’-tetramethylethylenediamine (TEMED, Sigma, St. Louis, USA) and the emulsion was degassed again for 6 min ...
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bioRxiv - Immunology 2021Quote: ... in conjunction with pLKO.1 lentiviral vector targeting GFP or human c-Rel at a ratio of (1.5:3:4) using X-tremeGENE HP DNA transfection reagent (Sigma). 48h and 72h later viral particle containing media was harvested ...
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bioRxiv - Microbiology 2021Quote: ... The labeled phages were gently shaked at room temperature for 3 hours and washed with an Amicon ultra-0.5 centrifugal filter unit (100kD, Millipore) in phage storage buffer (100mM NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by a one-hour blocking step using 0.1% triton-X with 3% Bovine Serum Albumin (BSA, Sigma A7906) in PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... or and 3) without 2i and LIF supplemented with 0.25 M all-trans-retinoic acid (Sigma-Aldrich, Cat #R2625), and cultured for 4 days.
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bioRxiv - Molecular Biology 2020Quote: ... Cell disruption was carried out by vortexing (3 cycles, 5 min each) at 4°C using 0.2 ml of glass beads (425-600 μm; Sigma). For the Rpb3 immunopurification ...
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bioRxiv - Microbiology 2021Quote: ... Fluids from a hydrothermal plume above Anemone and background seawater were collected using a Seabird SBE911 CTD and 10 L Niskin bottles and 3 L of the plume and seawater fluid was filtered through 0.22 μm Sterivex filters (Millipore). DNA was extracted from the filters using a phenol-chloroform method adapted from Crump et al ...
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bioRxiv - Microbiology 2021Quote: ... 200 μL of lysis buffer (8M urea in 50mM Tris-Cl pH 7.5, supplemented with phosphatase inhibitor cocktail 3 [Millipore-Sigma]) was added to each of the pellets and pipetted up and down several time to lyse a portion of the pellet ...
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bioRxiv - Molecular Biology 2020Quote: ... The target protein was eluted with 20 ml elution buffer 3 (600 mM NaCl, 20 mM Tris-HCl, pH 7.4, 2.5 mM desthiobiotin (Sigma-Aldrich), and 1 mM DTT).
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bioRxiv - Genomics 2021Quote: ... Antigen blocking and cell permeabilization were performed using blocking buffer consisting of 3% horse serum and 0.25% Triton X-100 (Sigma) in TBS for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed 3 times in PBS and blocked in PBS supplemented with 0.5 % fish skin gelatin (Sigma Aldrich) for 20 min ...
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bioRxiv - Immunology 2021Quote: ... Wells were washed and spots were detected by adding a filtered solution of 0.2mg/mL AEC (3-amino-9-ethylcarbazole; Sigma) in 0.1M sodium acetate buffer [pH 5.0] and 0.05% hydrogen peroxide (Sigma) ...
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bioRxiv - Bioengineering 2021Quote: ... and 1H-NMR spectra were recorded on a Varian Unity-300 (300MHz) NMR spectrometer (Varian Inc.) using 3-(trimethylsilyl)propionic acid-d4 sodium salt (0.05 w/v%, Sigma) as an internal standard (Figure S20 and Table S1).
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bioRxiv - Neuroscience 2021Quote: ... animals received daily intraperitoneal (I.P) injections of L3,4-dihydroxyphenylalanine methyl (L-DOPA, 3 day at 3mg/kg, then 6mg/kg, Sigma-Aldrich) and the peripheral DOPA decarboxylase inhibitor bensezaride hydrochloride (12mg/kg ...
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Disparate regulation of imd drives sex differences in infection pathology in Drosophila melanogasterbioRxiv - Immunology 2020Quote: Each sample consisted of 3 flies that were homogenised in 75μl of TE + 0.1% Triton X-100 (Sigma Aldrich). Samples were incubated for 20 min at 75°C and stored at −80°C ...
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bioRxiv - Neuroscience 2022Quote: ... The dopamine- synthesis inhibitor 3-Iodo-L-tyrosine (3IY; CAS: 70-78-0, Sigma Aldrich; concentration: 5 mg/ml) was added to samples of 2 ml yeast solution ...
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bioRxiv - Microbiology 2022Quote: ... the bottom channel of a polydimethylsiloxane (PDMS) device was precoated with fibronectin (3 μg/ml, Sigma-Aldrich, Cat# F1141). The microfluidic device was generated according to our previous report63 ...
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bioRxiv - Microbiology 2022Quote: ... Calu-3 cells (ATCC, HTB-55) were maintained in Eagle’s minimum essential medium (EMEM) (Sigma-Aldrich, Cat# M4655-500ML) containing 10% FBS and 1% PS ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were permeabilized with 0.01% Triton X-100 in PBS for 15 minutes and blocked with 3% bovine serum albumin (A7906, Sigma) in 0.01% Triton X-100 in PBS (blocking buffer ...
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bioRxiv - Microbiology 2023Quote: ... embryos were mechanically dechorionated and anaesthetized in 0.02 % (w/v) buffered 3-aminobenzoic acid methyl ester (pH 7.0) (Tricaine, Sigma-Aldrich). Zebrafish embryos were individually infected by microinjection with 1 nl of P ...
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bioRxiv - Bioengineering 2022Quote: ... 300 μL of supernatant was filtered under 3 kDa through Amicon Ultra-0.5 mL Centrifugal Filters (Millipore, Burlington, MA) or 96-well plates (Pall Corporation ...
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bioRxiv - Microbiology 2022Quote: ... cultures were pelleted by centrifugation at 1,600 rpm for 3 min and resuspended in a 10× volume of 5% sorbitol (Sigma), followed by incubation at 37°C for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... Calu-3 cells (ATCC, HTB-55) were maintained in Eagle’s minimum essential medium (EMEM) (Sigma-Aldrich, Cat# M4655-500ML) containing 10% FBS and 1% PS ...
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bioRxiv - Neuroscience 2023Quote: ... was concentrated to under 100 μL in 0.5 mL 3 kDa molecular weight cut-off centrifugal concentrators (Millipore, UFC500396) (Surface sample in Fig 4C) ...
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bioRxiv - Microbiology 2023Quote: ... The harvested cells were resuspended in immobilised metal chromatography (IMAC) buffer (50 mM Tris-HCl [pH 7.5], 300 mM NaCl) supplemented with 3 kU/ml rLysozyme (Novagen). The cell suspension was sonicated on ice (Ultrasonic disruptor UD-201 ...