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Citations for Millipore Sigma :
6001 - 6050 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Actin binding domain of Rng2 sparsely bound on F-actin strongly inhibits actin movement on myosin IIbioRxiv - Biophysics 2022Quote: ... This was followed by concentration with a centrifugal concentrator (Amicon Ultra-15 3 k device, Merck Millipore, Burlington, MA), and after supplementing with 10 mM DTT ...
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bioRxiv - Cell Biology 2022Quote: ... the slides were washed 3 times for 5 mins while gently shaking at RT with PBS + 0.1% Tween (Sigma) and stained for 90 mins in blocking buffer with a combination of secondary antibodies ...
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bioRxiv - Plant Biology 2022Quote: ... Br-3’,5’-cAMP (B5386) and Br-adenosine (B6272) were purchased from Sigma (Sigma-Aldrich, St. Louis, MO, USA). Br-2’,3’-cAMP was purchased from Biolog (Biolog Life Science Institute ...
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bioRxiv - Genetics 2022Quote: Fipronil (CAS# 120068-37-3) and Spinosad (CAS# 168316-95-8) were both obtained as analytical standards from Sigma. Insecticide bioassays were performed as described previously [20] ...
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bioRxiv - Molecular Biology 2022Quote: ... cut into smaller pieces and homogenize in 3 ml of nuclei isolation buffer (nuclei EZ lysis buffer (Sigma, NUC101), 1x Halt™ protease inhibitor cocktail (Thermo Fisher ...
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bioRxiv - Biochemistry 2022Quote: ... The purified proteins were concentrated to 2 mg/mL using centrifugal filters with either 3 kDa or 30 kDa cut-off (Amicon Ultra-0.5, Merck/Millipore) and the samples were centrifuged at 100,000 g ...
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bioRxiv - Neuroscience 2022Quote: ... GLASTCreERT2;Sun1-GFP mice at ∼3 weeks of age were intraperitoneally injected with 4-OHT (Sigma-Aldrich, #H6278-50mg) in corn oil (Sigma-Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... and 0.02% (w/v) yeast extract for 15N labelling or 15NH4C1 and 13C6 D-glucose (3 g/L, Sigma) with 0.02% (w/v ...
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bioRxiv - Bioengineering 2022Quote: ... organoids in Matrigel on day 3 or 4 after passage were incubated with 100 μM rhodamine 123 (Sigma-Aldrich) in HBSS (Wako ...
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bioRxiv - Biochemistry 2022Quote: ... The two aqueous streams were supplied with the cell solution and with a 3 μM substrate solution containing lysis agents (0.7× BugBuster protein extraction reagent, Merck Millipore; 60 kU/mL rLysozyme, Novagen) in droplet assay buffer ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting transformants were spotted on SD (−Leu, −Trp) and SD (−Trp, −Leu, −His) selection media containing different concentration of 3-amino-1,2,4-triazole (Sigma, A8056) as previously reported (Putarjunan et al. ...
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bioRxiv - Microbiology 2020Quote: ... Cultures were diluted to a final concentration of 1 × 106 CFU/mL in sterile 1X PBS and were stained in the dark for membrane potentials using 30 μM membrane potential indicator dye DIO2(3) (3,3=-diethyloxacarbocyanine iodide) (320684; Sigma) for 30 min ...
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bioRxiv - Cancer Biology 2021Quote: ... The protein pellet was washed 3 times in a solution of 0.3 M guanidine hydrochloride (Sigma, Cat. No. SRE0066) in 95% ethanol ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant containing Galectin-3 protein was treated with benzamidine sepharose (HiTrap Benzamidine FF, Sigma, Cat# GE17-5143-02) to bind and remove thrombin ...
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bioRxiv - Neuroscience 2020Quote: ... before being washed in PB 0.05 M and mounted on slides using chromium (3) potassium sulfate dodecahydrate (Sigma-Aldrich) and left to dry ...
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bioRxiv - Immunology 2020Quote: ... the sections were washed 3 times in PBS and permeabilized in PBS containing 0.3% Triton-X100 (#X100, Sigma-Aldrich) for 2 hours ...
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bioRxiv - Microbiology 2021Quote: ... Polarization of Calu-3 cells was evaluated by measuring the transepithelial resistance (TER) using a Millicell ERS meter (Millipore). Cells were considered polarized when TER values reached 500-600 Ωcm-2.
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bioRxiv - Neuroscience 2021Quote: ... sections were washed twice for 10-m in 1x PBS and then incubated for 1-hr in a blocking buffer containing 3-5% Normal Goat Serum (Jackson Immuno Research, 005-000-121) and 0.01-0.03% Triton-X (Sigma, X100), made up in 1x PBS ...
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bioRxiv - Biochemistry 2021Quote: ... The following mobile phases were used for FIA-MS scouting: 20 mM formic acid pH 3 (Sigma-Aldrich F0507), 10 mM ammonium acetate pH 5 (Sigma-Aldrich 73594) ...
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bioRxiv - Neuroscience 2021Quote: ... Fish were deeply anesthetized with 0.2% 3-aminobenzoic ethyl ester (MS-222; Sigma-Aldrich, St. Louis, MO; RRID: SCR_008988) in water just before surgery or tissue preparation.
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bioRxiv - Neuroscience 2021Quote: ... and posteriorly permeabilized and blocked against nonspecific binding using a solution of 3% bovine serum albumin (BSA) (Sigma-Aldrich) and 0.1% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... and then concentrated using 15 ml Amicon spin filter cartridges (cutoff: 3 kDa, 10705884-Merck Millipore, Burlington, MA, USA) by serial addition of 10 ml of CM and centrifugation at 4000 x g until 1.5 ml were left ...
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bioRxiv - Biochemistry 2021Quote: ... One milliliter of the N-oxide solution was added dropwise to 3 mL of sodium acetate (37.5 mM; pH 5.3; Sigma Aldrich), followed by incubation at room temperature for 24 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... The unbroken cells were removed by centrifugation (3,000 x g; 4°C; 5 min; Sigma 3-16KL; rotor 11180). The membrane fraction was pelleted down by high-speed centrifugation (100,000 x g ...
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bioRxiv - Microbiology 2021Quote: ... the lysates were incubated for 3 h with 50 μl anti-FLAG packed agarose affinity gel (A-2220; Sigma) at 4 °C with rotation ...
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bioRxiv - Neuroscience 2020Quote: Sections were washed in 0.1M PBS solution before blocking by incubation with 3% normal donkey serum (NDS, Sigma; D9663), 0.2% PBS-Triton for 2 hours ...
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bioRxiv - Neuroscience 2022Quote: ... N2a NM-HAagg (EP) donor cells were treated for 3 d with methyltransferase inhibitors 5-Azacytidine (Aza) (Sigma-Aldrich), Decitabine (Dec ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 250 µL X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) (20 mg/ml) (Sigma-Aldrich, UK) and Isopropyl ß-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Neuroscience 2022Quote: A model of hyperalgesic priming was used to induce chronic widespread pain in mice with consecutive injections of carrageenan into the right gastrocnemius muscle 4 days apart (i.m. 3% 30μl, Sigma Aldrich). Control mice only received one injection on D0 (30).
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bioRxiv - Immunology 2022Quote: ... and concentrated until ~1.5 mg/ml using Amicon Ultra-15 Centrifugal Filter Unit with a 3 kDa cut-off (Millipore). Protein concentration was determined by BCA assay (Pierce/Thermo Scientific).
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bioRxiv - Immunology 2022Quote: ... MSCs were frozen at passage 3 with 1.25*106 cells/ml in freezing medium containing FBS (BCBV7611, Sigma-Aldrich) with 10% DMSO and stored in liquid nitrogen until further use ...
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bioRxiv - Immunology 2022Quote: ... and either plate-bound 3 μg/mL mouse IgG1 isotype control or anti-FLAG mAb (clone M2, Sigma-Aldrich) in complete RPMI-1640 culture medium supplemented with 10 μM BrdU ...
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bioRxiv - Immunology 2022Quote: ... and either plate-bound 3 μg/mL mouse IgG1 isotype control or anti-FLAG mAb (clone M2, Sigma-Aldrich) in complete RPMI-1640 culture medium ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining pellet was resuspended in 500 μL bovine serum albumin (BSA, 3% solution in PBS, Sigma-Aldrich, A3294) and centrifuged again at 1000xg at 4°C for 10 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... – to synchronise cells at the G2/M boundary - or with a 3-hour treatment with STLC (10μM, Sigma-Aldrich) - to arrest cells in mitosis with monopolar spindles ...
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bioRxiv - Neuroscience 2022Quote: ... then selected after 24-48 hours for 3 to 14 days with 8 µg/mL of puromycin (Sigma-Aldrich). The iPSCs with a stably integrated human NIL under a tetracycline-inducible promoter were exposed to 2 µg/mL doxycycline in neuronal induction medium (NIM) ...
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bioRxiv - Bioengineering 2022Quote: ... a differentiation medium (reduced serum + additives) adapted from Messmer et al.3 – reduced serum with insulin (Sigma Aldrich #I0516), 1-oleoyl lysophosphatidic acid (LPA ...
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bioRxiv - Developmental Biology 2022Quote: ... 50,000 of cells for each experimental replicate and/or condition were resuspended in lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2 and 0.1% IGEPAL CA-630, Sigma aldrich) to perform crude nuclei isolation ...
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bioRxiv - Biochemistry 2020Quote: ... The empty liposomes were prepared from a mix of DOPC (1,2-Dioleoyl-sn-glycero-3-phosphocholine) (Avanti lipids) and CHS (Sigma). DOPC and CHS were dissolved in chloroform at a 10:1 (wt:wt ...
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bioRxiv - Immunology 2020Quote: ... 50 μl of antibody exposed to hemin or hemin in PBS only were mixed with 150 μl of reaction buffer (0.15 M citrate-phosphate buffer pH 5) containing 0.9 mM of 2,2-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS, Sigma-Aldrich) and 6 mM H2O2 ...
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bioRxiv - Molecular Biology 2020Quote: ... was obtained by the transfection of shRNA directed against the 3’ UTR region of Aurora-A (clone NM_003600.x-985s1c1, Sigma) followed by clonal dilution and selection of Aurora-A depleted cells with 2 μg/ml of puromycin.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 12 mg RBD protein in 3 mL PBS was mixed with equal-volume Freund’s incomplete adjuvant (Sigma-Aldrich) for the second boosting ...
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bioRxiv - Cell Biology 2020Quote: ... and gravity-sedimented for 3-5 min onto coverslips coated with poly-D lysine (Sigma-Aldrich, catalog number P1024), until cells became in contact with the glass ...
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bioRxiv - Developmental Biology 2021Quote: ... and replated in neural maintenance media (NES) with 10μM Y27632 at 3×105 cells per cm2 on laminin (Sigma) coated surface ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were broken by vortexing (3 cycles, 5 min each) using 0.2 ml of glass beads (425-600 μm; Sigma)) ...
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bioRxiv - Molecular Biology 2020Quote: ... Embryos/larvae were kept in egg water (60 μg/ml Instant Ocean sea salts) at 28.5°C and treated with 0.02% ethyl 3-aminobenzoate methanesulfonate (Tricaine, Sigma-Aldrich) for anesthesia before imaging and fixation ...
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bioRxiv - Microbiology 2021Quote: ... Wells with agarose plugs were fixed 3 days later with a solution of 2.5% formaldehyde (1635-4L, Sigma-Aldrich) in 3x PBS ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 1.47 mM KH2PO4 supplemented with 5 mM DTT and concentrated to ∼10-20 mg/mL using 3 kDa Millipore concentrator and stored at -80 °C.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... we carried out final desalting and buffer equilibration using ultrafiltration (Amicon centrifugal filters, 3-kDa molecular weight cutoff, Millipore). For oligonucleotides administered in PBS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: 36 hpf or 66 hpf embryos were anesthetized with 112 μg/mL 3-aminobenzoic acid ethyl ester (tricaine, Sigma), immobilized in 1% low melting-point agarose in the center of a 35 mm glass-bottomed dish (Corning) ...