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Citations for Millipore Sigma :
6101 - 6150 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were anaesthetised in a solution of buffered ethyl 3-aminobenzoate methanesulfonate salt (100 mg/L; MS-222, Sigma) in seawater ...
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bioRxiv - Immunology 2022Quote: ... 7.5×107/15ml RPMI+3%FBS and stimulated with 25nM phorbol 12-myristate 13-acetate (PMA; Millipore-Sigma #P8139) Plates were incubated at 37°C ...
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bioRxiv - Immunology 2022Quote: ... 7.5×107/15ml RPMI+3%FBS and stimulated with 25nM phorbol 12-myristate 13-acetate (PMA; Millipore-Sigma #P8139) Plates were incubated at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... then fixed with 4% paraformaldehyde for 30 min at room temperature and washed 3 times in PBS.Cells were permeabilized with 0.1% Triton-X100 (Sigma T8787) and washed 3 times in PBS ...
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bioRxiv - Immunology 2023Quote: ... which was precoated for 3 hours at 37°C with 4ug/mL fibrinogen (Sigma-Aldrich; Saint Louis, MO, USA). After letting the cells settle for 3 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-tagged CLAMP proteins were eluted for 3 h at 4°C on a rotating wheel in the presence of 0.5 mg/ml FLAG-Peptide (Sigma) in 1 ml Buffer C containing 1x PIC ...
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bioRxiv - Bioengineering 2023Quote: ... they were incubated with 4 mg/mL of N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC; Sigma-Aldrich, Schnelldorf, Germany) dissolved in 100 mM 2-(N-morpholino ...
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bioRxiv - Biophysics 2023Quote: ... washed 3×20min with milli-Q water and stained overnight with Coomasie Brillant Blue (G250, Sigma, Saint-Louis, USA). They were finally rinced 3×20 min with milli-Q water.
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bioRxiv - Microbiology 2023Quote: ... Calu-3 cells (ATCC, HTB-55) were maintained in Eagle’s minimum essential medium (EMEM) (Sigma-Aldrich, Cat# M4655-500ML) containing 10% FBS and 1% PS ...
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bioRxiv - Microbiology 2023Quote: ... Zebrafish larvae were anesthetized with egg water containing 0.02% buffered 3-aminobenzoic acid ethyl ester (Tricaine, Sigma-Aldrich, Netherlands) for bacterial infection and imaging experiments.
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bioRxiv - Immunology 2023Quote: ... and fixed for 3 hr at room temperature (RT) in freshly prepared Carnoy’s fixative [60% anhydrous methanol (Sigma-Aldrich), 30% chloroform (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... slices were then blocked and permeabilized in 3% BSA in PBS containing 0.1% Triton X-100 (Sigma-Aldrich # T8787) and stained with rabbit anti-GFP (2 μg/mL ...
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bioRxiv - Immunology 2023Quote: ... Tumors were digested for 45 minutes with gentle shaking at 37°C in HBSS containing 3 mg/ml Collagenase IV and 2.8 mg/ml DNase-I (Sigma). Cells from BRAFi (n = 5 ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was concentrated and using using Amicon Ultra-15 centrifugal filters (3 kDa cut-off, Merck-Millipore) and then buffer was exchanged to buffer B (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Neuroscience 2023Quote: Cells in the tectum were transfected using in vivo electroporation of stage 46 animals anesthetized in 0.01%MS-222 (3-aminobenzoic acid ethyl ester, Sigma) dissolved in Steinberg’s media ...
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bioRxiv - Neuroscience 2023Quote: ... 3-5 organoids per condition were dissociated by incubation with a solution of 0,5 mg/ml Collagenase/Dispase (Sigma) + 0,22 mg/ml EDTA (Euroclone ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then permeabilized with 0.1% Triton-X for 10 min and blocked with 3% goat serum (GS) (Sigma) for 30 mins at room temperature (unless stated otherwise) ...
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bioRxiv - Biophysics 2023Quote: ... NMR samples were buffer exchanged into NMR buffer (pH 7.4 or pH 7.8) using Amicon Ultra-15 centrifugal concentrators (3-kDa cutoff, Millipore Sigma) to a final nucleic acid concentration of ∼1 mM ...
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bioRxiv - Neuroscience 2023Quote: ... tissues were washed 3 times with PBS-T (0.2% Triton X-100 in PBS (PBST) with 0.01% sodium azide (Sigma Aldrich)) and blocked for 1 hour in 10 % Goat Serum (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-tagged MSL proteins were eluted for 3 h at 4°C on a rotating wheel in the presence of 0.5 mg/ml FLAG-Peptide (Sigma) in 1 ml Elution buffer ...
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bioRxiv - Microbiology 2023Quote: ... wells were incubated with 200 µL of a blocking buffer (PBS - Bovine Serum Albumin 3% (BSA reference: A9418, Sigma)) during one hour at 35°C ...
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bioRxiv - Neuroscience 2023Quote: ... by dispensing individual cells in 5 nL drops directly into 3 µL ice-cold single cell lysis buffer (scLB, 0.134% Triton X-100 [Sigma] ...
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bioRxiv - Neuroscience 2023Quote: Estrus was induced in ovariectomized females with a subcutaneous injection of 10 µg β-estradiol 3-benzoate (E2) (Sigma) in 0.05 mL sesame oil ∼48 hours before testing ...
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bioRxiv - Microbiology 2023Quote: ... permeabilised with 0.1% Triton X-100 in PBS for 10 minutes and blocked in 3% BSA (Sigma Aldrich, #A9418) for 1 hour at RT ...
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bioRxiv - Microbiology 2024Quote: ... The antigen-bound magnetic beads were washed five times with lysis buffer and eluted with 2.5 volumes of 3× FLAG peptide (150 ng/μl, Sigma) for 3 h at 4°C with gently shaking ...
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bioRxiv - Neuroscience 2022Quote: ... Passive dextran permeability was measured by culturing iBECs on Ø 0.4 μm pore polyester or polycarbonate Transwell inserts and cells were exposed to 0.5 mg/mL fluorescein isothiocyanate (FITC)-conjugated 3 – 5 kDa dextran (Sigma) for 24h ...
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bioRxiv - Cell Biology 2022Quote: ... 6- to 8-week-old mice of the indicated genotypes were intraperitoneally injected with 2.5 ml 3% thioglycollate (T9032, Sigma). On day 4 after the thioglycollate injection ...
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bioRxiv - Neuroscience 2022Quote: ... then selected after 24-48 hrs for 3 to 14 days with 8 µg/mL of puromycin (Sigma-Aldrich).
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bioRxiv - Plant Biology 2022Quote: Analytical reference standards were used for the following analytes: indole-3-acetic acid (IAA; Sigma-Aldrich St. Louis, MO), N-(3-indolylacetyl)-DL-aspartic acid (IAA-Asp ...
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bioRxiv - Bioengineering 2022Quote: ... MSCs were frozen at passage 3 with 1.25*106 cells/ml in freezing medium containing FBS (BCBV7611, Sigma-Aldrich) with 10% dimethylsulfoxide (DMSO ...
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bioRxiv - Biochemistry 2024Quote: Exoproteome fractions were concentrated to 1 mg mL-1 total protein (approximately 10 times concentration) using an Amicon Ultra-0.5 Centrifugal Filter Unit with a molecular weight cutoff of 3 kDa (Merck Millipore). A 10 µL aliquot of the concentrated exoproteome fraction was reduced with 5 mM tris (2-carboxyethyl ...
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bioRxiv - Biochemistry 2024Quote: ... Colour was developed by adding 100 µL of 3 mg/mL disodium 4-nitrophenyl phosphate solution (DNPP, Sigma Aldrich) dissolved in 100 mM Tris ...
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bioRxiv - Molecular Biology 2024Quote: ... The linear DNA backbone was then extracted from the gel via electroelution in D-tubes (EMD Millipore 71508-3), followed by phenol chloroform extraction and butanol concentration.
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bioRxiv - Neuroscience 2024Quote: ... The device was then pulsed with 3 bolus each of 4 different concentrations of dopamine hydrochloride (Sigma, H8502-10G), 0.5 μM ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by staining of ossified bones with a 50 mg/l of alizarin red S (Sigma, #130-22-3) solution in 2% KOH for 3 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... reconstituted in 3 µL of 66% acetonitrile and analyzed by a ZIC-HILIC column (150 × 2.1 mm, EMD Millipore) coupled with a Q Exactive HF Orbitrap MS (Thermo Fisher ...
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bioRxiv - Biophysics 2024Quote: ... Mice were decapitated and the nose was dissected en bloc and embedded in 3% low-grade agar (A7002, Sigma) prepared with Ringer’s saline solution containing (in mM) ...
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bioRxiv - Cancer Biology 2024Quote: ... HUVECs were seeded into the collagen gel channel with media containing 3% dextran (Leuconostoc spp., Mr 450,000–650,000, Sigma-Aldrich) at a density of 1 × 107 cells/mL ...
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bioRxiv - Cell Biology 2024Quote: ... Flat mounts were incubated in 300 µL of blocking buffer (3% (W/V) bovine serum albumin (BSA) (Catalog #BP9703-100) and 0.1% (V/V) Triton X-100 (Sigma) in HBSS (Fisher Scientific Catalog # MT21023CV ...
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bioRxiv - Microbiology 2024Quote: ... Coloring reactions were performed with nitro blue tetrazolium/5-Bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) solution (Sigma), and then sections were washed in PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... were incubated with 3 µg of D-Val-Leu-Lys 4-nitroanilide dihydrochloride chromogenic substrate (S-2251) (Sigma-Aldrich) in the presence of 1 µg of rFhENO or 1% BSA as negative control in a test volume of 100 µL ...
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bioRxiv - Plant Biology 2024Quote: ... After immunolabeling the coverslips were incubated in Acryloyl-X-SE 0.1 mg/mL in PBS (ThermoFischer) overnight at RT and then in the monomer solution (11.7% NaCl, 3 % acrylamide (Sigma), 0.15% N,N′-methylenebisacrylamide (Sigma) ...
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bioRxiv - Neuroscience 2024Quote: ... before being cut into 250μm parasagittal slices using a McIlwain tissue chopper and placed on Millicell membrane (3 to 4 slices each per animal, 0.4 μm membranes, Merck Millipore) in 50% BME (41010026 ...
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bioRxiv - Microbiology 2024Quote: ... Liver and spleen samples from experimentally infected fish for 3 days were fixed in 4% paraformaldehyde (PFA, Sigma, USA), embedded in paraffin and sectioned (4 μm ...
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bioRxiv - Molecular Biology 2023Quote: ... pregnant females were ovariectomized on E2.5 and afterwards injected with 3 mg medroxyprogesterone 17-acetate subcutaneously (Sigma, M-1629) on E3.5 and E5.5 ...
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bioRxiv - Cell Biology 2024Quote: ... were washed 3 x with PBS followed by incubation with 20 μg mouse anti-FLAG M2 Ab (Sigma Aldrich) for 2 h at 4 °C with rotation prior to 3 x PBS washes and resuspension in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... permeabilized using 0.2% Triton-X100 in 1x phosphate buffered saline (PBS) for 15 min and blocked using 3% BSA (Sigma) in 1x PBS for 30 min ...
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bioRxiv - Immunology 2024Quote: ... PCR was performed to add the 3XFlag tag to the N terminus of ZBP1 cDNA at the 5’ Not1 site and 3’ Sal1 site to facilitate cloning into expressing vector p3XFlag-CMV-7.1 (Sigma). The resulting plasmid containing the 3XFlag-ZBP1 was further subcloned in the Bst1 and BamHI sites of the vector pLVX-EF1α-AcGFP1-C1 (Takara ...
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bioRxiv - Microbiology 2024Quote: ... The female individuals were anesthetized with 0.02% (w/v) ethyl 3-aminobenzoate methanesulfonate (Sigma-Aldrich, St. Louis, MO, USA), euthanized by decapitation ...
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bioRxiv - Microbiology 2024Quote: ... Oat seedlings for purification and amplification were treated with 3 mM maleic hydrazide (Sigma-Aldrich, St. Louis, MO, USA) at emergence ...