Labshake search
Citations for Millipore Sigma :
5951 - 6000 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... and Atto 647N-labeled 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (Atto 647N DPPE, ≤90.0%) were purchased from Sigma-Aldrich. Gibco™ Dulbecco’s Modified Eagle’s Medium (DMEM ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were plated by pipetting (3 μl) or using a pinning tool (Sigma-Aldrich, St. Louis, MO, R2383-1EA) on control and selective media to assess specific phenotypes ...
-
bioRxiv - Molecular Biology 2021Quote: ... to 7.0 with HCl) for 45 minutes and a final wash in in 2x SSC (3 M NaCl (Sigma), 0.3 M sodium citrate (Sigma) ...
-
bioRxiv - Molecular Biology 2021Quote: An RNA oligonucleotide (5’ -UUUUCAUGCUACGCGUAGUUUUCUACGCG- 3’) with Cyanine 5.5 at the 5’-end was obtained from Millipore Sigma (USA). Prior to the reaction ...
-
bioRxiv - Developmental Biology 2022Quote: ... amiloride (EIPA) (A3085), LiCl and Sodium chloride (NaCl) (S9888), IPA-3 (I2285, used at 2.5 μM) were obtained from Sigma. Bafilomycin A1 (Baf-A1 ...
-
bioRxiv - Bioengineering 2021Quote: Sections were incubated in a permeabilization and blocking solution of 0.3% Triton X (A4975, PanREAC AppliChem) and 3% BSA (A7906, Sigma) in PBS for 24 hours at 4°C ...
-
bioRxiv - Plant Biology 2020Quote: ... 10 ml of alkaline phosphatase (100mM Tris-HCl pH 9.5, 100 mM NaCl, 3 mM MgCl2) containing 80 μl NBT (Sigma) (35mg/ml in70% DMSO and 30 μl de BCIP (Sigma ...
-
bioRxiv - Neuroscience 2020Quote: ... sections were incubated overnight at 4°C in a humid chamber with primary antibodies (Table 3) in 0.1 M PBS containing 10% normal horse/goat serum and PBS-0.3% Triton X-100 (Sigma-Aldrich). After primary antibody incubation ...
-
bioRxiv - Bioengineering 2021Quote: ... pH 7.4 by centrifugation through 3 kDa molecular weight cut off centrifugal filters (Amicon Ultra®, Millipore, Dublin, Ireland). Absorbance at 555 and 280 nm was measured for labelled samples and calculations were performed according to manufacturer’s instructions using an arbitrary extinction coefficient of 100,000 and molecular mass of 100,000 to enable quantification of relative protein concentration and label substitution efficiency ...
-
bioRxiv - Bioengineering 2020Quote: ... we added 3% v/v photoinitiator Lithium phenyl-2,4,6-trimethylbenzoylphosphinate (Sigma-Aldrich, “LAP”, 39.2 mg/mL in DI water). Produced Ln/polymer droplets were collected through Tygon tubing into a 24-well plate (Thermo Fisher Scientific) ...
-
bioRxiv - Biochemistry 2021Quote: ... after a 60 min incubation at 25 °C the reactions were stopped by addition of 3 μl 3.3 × LDS sample buffer (Sigma) containing NEM (40 mM final concentration ...
-
bioRxiv - Neuroscience 2020Quote: ... Cells were released via 0.12% trypsin without EDTA and washed 3 times with PBS (Sigma-Aldrich, St. Louis, MO). 0.4% trypan blue (Thermofisher Scientific ...
-
bioRxiv - Neuroscience 2021Quote: ... Adult zebrafish (6 female and 6 male) were anesthetized in 0.02% tricaine (E10521, Ethyl 3-aminobenzoate methanesulfonate; Sigma-Aldrich). Shallow lesions were made on the skin (10 on each side ...
-
bioRxiv - Neuroscience 2020Quote: ... A 96-well PCR plate was prepared with one 3 mm diameter borosilicate solid-glass bead (Millipore Sigma #Z143928) and 100 µl PBS in each well ...
-
bioRxiv - Neuroscience 2021Quote: ... and immediately fused to 18 mm glass coverslips (#1.5) that had been treated with 3-aminopropyl-triethoxysilane (Sigma 440140). Samples were washed with PBS 3x 5 min ...
-
bioRxiv - Neuroscience 2020Quote: ... The rest was incubated with 30μl of Protein G Dynabeads and 3 μl of anti-FLAG M2 antibody (Sigma) overnight to immunoprecipitate (IP ...
-
bioRxiv - Cancer Biology 2021Quote: ... gRNA sequence: 5’-ACTTGGCCAAGGAGTACGG-3’) in line with GFP from the (p04) U6-gRNA:CMV-eCas9-2a-tGFP plasmid (Sigma). One day later ...
-
bioRxiv - Cell Biology 2021Quote: ... and cells were isolated by digesting the pulp tissue with collagenase type I (3 mg/ml, Sigma-Aldrich, Germany) for 60 minutes at 37°C ...
-
bioRxiv - Biophysics 2020Quote: ... the culture medium was removed and cells were rinsed 3 times with Dulbecco’s Phosphate Buffered Saline (DPBS, Sigma-Aldrich).
-
bioRxiv - Cancer Biology 2020Quote: ... CM (and the same volume of SM as control) was then concentrated by 3 kDa Amicon filters (Merck-Millipore) to a final volume of 100 μl/mouse and stored at −20°C until usage ...
-
bioRxiv - Cell Biology 2021Quote: ... The target cell lines at 50-60% confluency were then infected using 3 mL virus with polybrene reagent (Sigma). After 24 hours ...
-
bioRxiv - Physiology 2021Quote: ... Mice were fed ad libitum or fasted at ZT0 and given CL316243 (3 mg/kg body weight; Sigma Aldrich) or vehicle (0.5% carboxymethylcellulose in sterilized water ...
-
bioRxiv - Biochemistry 2021Quote: ... The C-terminal domain (CTD) of DRH-3 (residues 940-1108) was cloned into the expression vector pET15b (Novagen), and the resulting clone carries an N-terminal His6 tag and a thrombin cleavage site ...
-
bioRxiv - Biochemistry 2020Quote: ... each N-Cdh sample was desalted against PBS using Amicon centrifugal filters with a 3 kDa molecular weight cutoff according to the manufacturer’s protocol (Merck-Millipore). Unprocessed xCGE-LIG N-glycocomics raw data are available upon request.
-
bioRxiv - Bioengineering 2021Quote: ... 0.1% solution of Triton X-100 in PBS (v/v) and 3% Bovine Serum Albumin solution (Sigma Aldrich, A3803) in PBS (w/v ...
-
bioRxiv - Cell Biology 2021Quote: ... the surfaces of the coverslips were amino-modified with 3-(Trimethoxysilyl)propyl methacrylate (#M6514, Sigma-Aldrich, Saint-Louis, USA) to allow firm gel attachment ...
-
bioRxiv - Cell Biology 2021Quote: ... Fixed cells were then rinsed 3 times with PBS followed by permeabilization with 0.1% triton X-100 (Sigma, X100) in PBS for 10 minutes at room temperature ...
-
bioRxiv - Cell Biology 2021Quote: For analysing autophagy flux 72-hours old larvae were transferred to fresh medium containing 3 mg/ml chloroquine (Sigma) as described previously (Low et al. ...
-
bioRxiv - Plant Biology 2021Quote: ... Polysomes were eluted by resuspension of the washed gel in polysome wash buffer containing 3×FLAG peptide (Sigma-Aldrich). Total RNA was extracted from the final elution using Direct-zol RNA kit (Zymo research) ...
-
bioRxiv - Plant Biology 2020Quote: ... Trypsin digestion was stopped either by heating at 95 °C for 3 min or by ultrafiltration using 0.5 ml Amicon Ultra centrifugal devices (20 kDa MWCO, Millipore). A five microliters aliquot was kept for MALDI-TOF MS analysis and the rest was used for the enrichment of biotinylated peptides by affinity chromatography.
-
bioRxiv - Plant Biology 2021Quote: ... Col-0 plants grown on soil for 3-4 weeks were spray-treated with 0.5 mM SA (Sigma-Aldrich) and tissues harvested at indicated time-points for further analysis.
-
bioRxiv - Plant Biology 2020Quote: ... The algal suspension was mixed into an equal volume of 3% (w/v) sodium alginate (Sigma, Product number 180947) solution then a syringe was used to generate 3 – 4 mm diameter algal-alginate beads by adding drops of the sodium alginate-algal mixture into a 2% (w/v ...
-
bioRxiv - Plant Biology 2021Quote: ... The remaining 200 µL was transferred to a 3 kDa Amicon Ultra 0.5 mL filtering device (Millipore, Burlington, MA) for the quantification of AAs ...
-
bioRxiv - Microbiology 2021Quote: ... amoebae were left unstained and incubated with either 3×106 or 1.5×107 fluorescent red carboxylate-modified polystyrene 2.0 μm latex beads (Sigma-Aldrich) or incubated in the absence of latex beads ...
-
bioRxiv - Microbiology 2020Quote: ... Plates were then washed 3 times with PBST before the addition of 100ul of .5mg/ml o-Phenylenediamine (Sigma) diluted in phosphate-citrate buffer with sodium perborate (Sigma) ...
-
bioRxiv - Cell Biology 2021Quote: ... were immobilized on freshly made 3% agarose pad in a drop of M9 media containing 0.4 mM (0.05%) tetramisole (Sigma Aldrich) and 3.8 mM (0.5% ...
-
bioRxiv - Microbiology 2021Quote: ... 50 µg of purified Cu-LpmP or CslZ protein were incubated for 3 h at room temperature with 0.2% peptidoglycan (PG) from Bacillus subtilis (Sigma), 5 mg α-chitin from shrimp shells (Sigma ...
-
bioRxiv - Microbiology 2021Quote: Cells transfected with NL-43 or pNL4-3-△5’ were lysed using freshly made lysis buffer (0.5% Igepal CA-630, Sigma; 50 mM Tris ...
-
bioRxiv - Plant Biology 2021Quote: Seedlings were germinated vertically for 3 days on half strength MS plates containing 0.4% Gelzan™ CM (Millipore Sigma), and then transferred to plates containing either 0 (-P ...
-
bioRxiv - Plant Biology 2021Quote: ... Fractions were desalted on C18 Cartridges (Empore™ SPE Cartridges C18, bed I.D. 7 mm, volume 3 ml, Sigma), and reconstituted in 0.1% (v/v ...
-
bioRxiv - Cancer Biology 2020Quote: ... MD2G (3 μg) and pLKO-LV-gene-specific (6 μg) plasmids in the presence of PEI transfection reagent (Sigma). The generated lentiviral particles were collected 24 hours post-transfection ...
-
bioRxiv - Cell Biology 2021Quote: ... series was made in a 96-well plate and cells were spotted onto YPD or YPD + 500μM auxin (indole-3-acetic acid dissolved in DMSO; Sigma) plates ...
-
bioRxiv - Cell Biology 2020Quote: ... Media was changed every 3 days and cells were fixed two weeks after initial seeding with 0.5% glutaraldehyde (Sigma) in PBS for 20 min and left drying overnight ...
-
bioRxiv - Biophysics 2020Quote: The GYMC52_3505 and pMHTDelta238 plasmids were transformed to Rosetta2(DE3)pLysS competent cells (Cat. No. 71401-3, EMD Millipore). Transformation reactions were spread on LB plates with ampicillin (Amp ...
-
bioRxiv - Cell Biology 2020Quote: ... in DMEM solution for two 45 minute periods and transferred to 0.5 mg/mL Collagenase D in DMEM solution supplemented with 3% Liberase TL (Sigma) overnight ...
-
bioRxiv - Cell Biology 2020Quote: ... Cell medium was replaced with 3 mL medium containing 20 μL of the purified lentivirus and 3μl polybrene transfection reagent (Merck Millipore). Medium was supplemented with 10 µg/mL puromycin for selection of successfully transduced cells two to three days after transduction.
-
bioRxiv - Cell Biology 2021Quote: ... cells were washed 3 times in PBS and blocked in PBS supplemented with 0.5% fish skin gelatin (Sigma-Aldrich) for 20 min ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were then washed 3 times in staining buffer (PBS with 0.1% Bovine Serum Albumin (cat. num. A8806, Sigma), 0.05% NaN3 (cat ...
-
bioRxiv - Cell Biology 2022Quote: ... Coverslips were finally washed 3 times in PBS-Tween 20 0.1% and mounted in Mowiol 4-88 (81381, Sigma).
-
bioRxiv - Biophysics 2022Quote: PC-3 cells were seeded at 1,400 cells/100 μL in 96 well plates in RPMI media (Sigma-Aldrich) with 10% FBS ...