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Citations for Millipore Sigma :
5901 - 5950 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: Adult zebrafish (3 months post fertilization) were euthanized using a 25x tricaine solution (4000 mg tricaine powder (Sigma), 979 ml distilled water ...
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bioRxiv - Microbiology 2024Quote: ... the animal’s drinking water was replaced from days 3-8 with water containing 5% L-arabinose (Sigma Aldrich). InducTn-seq samples were collected 8 days post inoculation by plating fresh feces on solid LB containing 50 µg/ml kanamycin ...
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bioRxiv - Microbiology 2024Quote: ... They were washed three times with PBS and blocked with 3% bovine serum albumin (BSA) (Sigma-Aldrich, A3294) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was applied to 3 kDa MWCO filters (Amicon Ultra-0.5 Centrifugal Filter; Merck Millipore, Darmstadt, Germany), and centrifuged at 14,000 × g for 45 min at 4°C ...
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bioRxiv - Physiology 2024Quote: ... The samples were separated on a Discovery HS F5-3 column (2.1 mm I.D. x 150 mm L, 3μm particle, Sigma-Aldrich) using a step gradient of mobile phase A (0.1% formate ...
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bioRxiv - Neuroscience 2024Quote: ... Fish aged 3-30 dpf were mounted dorsal side up in 1.5% low-melting temperature agarose (Sigma-Aldrich) on a glass bottom dish glued to the bottom of a petri dish chamber ...
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bioRxiv - Neuroscience 2024Quote: ... larval fillets were incubated in 0 Ca2+ modified HL-3 supplemented with 50 μM EGTA-AM (Sigma-Aldrich) for 10 min ...
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bioRxiv - Cancer Biology 2024Quote: ... were lysed in ice-cold lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% NP-40: Sigma). Transposition was performed directly on nuclei using 25 μl tagmentation reaction mix (Tagment DNA Buffer #15027866 ...
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bioRxiv - Cell Biology 2024Quote: ... That plasma was transferred into a 15 mL conical tube containing 3 mL of Ficoll-Paque Plus (Sigma), taking great care to avoid mixing the two solutions ...
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bioRxiv - Bioengineering 2024Quote: ... samples were washed three times in PBS and permeabilised with 3% bovine serum albumin (A7906, Sigma Aldrich, Germany). Samples were stored in PBS at 4° C until further analysis.
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bioRxiv - Cell Biology 2024Quote: ... and DEA buffer solution (1M diethanolamine, 0.5 mM MgCl2, pH 9.8) containing 3 mg/ml of alkaline phosphatase substrate (Sigma). Absorbance was measured at 405 nm (Varioskan LUX reader).
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bioRxiv - Cell Biology 2024Quote: ... BMDMs was primed with LPS (100 ng/mL) for 3 hours followed by Nigericin (10 mM, Sigma; N7143) treatment ...
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bioRxiv - Cancer Biology 2024Quote: ... filtered culture supernatant from BHF cells (from Dr. George Kassiotis) and 3 ug/mL Polybrene (SIGMA H9268-5G). BHF cells are a Mus dunni tail fibroblast (MDTF ...
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bioRxiv - Cancer Biology 2024Quote: ... 25,000 ZR75.1 and 25,000 ZRR cells were seeded into their respective wells in 3% PRFMEM media (Sigma Aldrich). No cells were seeded into the outer wells of the plate ...
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bioRxiv - Cell Biology 2024Quote: ... The membranes were blocked with 3% bovine serum albumin (BSA) (Sigma-Aldrich, St. Louis, MO, USA, Cat# A7906) in Tris-Buffered Saline with Tween 20 (TBS-T ...
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bioRxiv - Cell Biology 2024Quote: ... zebrafish larvae were added to E3 media containing 0.08 mg/mL tricaine (MS222/ethyl 3-aminobenzoate; Sigma-Aldrich). Once non-motile ...
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bioRxiv - Developmental Biology 2024Quote: ... Treated wells received basal media supplemented with 3 µg/mL (5.8 µM) of doxycycline hyclate (Sigma, D5207-1G). This concentration was chosen as it represents drug blood serum levels during doxycycline oral treatment115,116 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... pyrophosphatase solution [NEB] (0.1 Unit/ml) and polyethylene glycol (PEG-8000; Sigma-Aldrich, Cat. No. 25322-68-3) to a final concentration of 5% (w/v)— was poured over the gel ...
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bioRxiv - Developmental Biology 2024Quote: ... 200 µl of DPBS (without Calcium and Magnesium) containing 3% Bovine serum albumin (BSA) (Sigma-Aldrich, cat. A3311) were added to quench the trypsin action and immediately aspirated to remove the trypsin ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were fixed with 3.7% paraformaldehyde and washed 3 times with PBS before and after incubation with mouse anti-FLAG M2 primary antibody (Sigma, diluted 1:2000 in PBS with 1% BSA) or HRP-conjugated anti-mouse IgG secondary antibody (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... for 3 min and then placed in a solution of 1% w/v Alcian blue in 3% acetic acid (pH 2.5) for 30 min (EMD Millipore, Cat. No. TMS-010-C). Slides were then rinsed in 3% acetic acid and allowed to dry overnight ...
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bioRxiv - Molecular Biology 2020Quote: Ultrafine anaphase bridges in U2OS osteosarcoma cells exogenously expressing wild-type POT1 and POT1-ΔOB were detected using the mouse monoclonal anti-PICH antibody (clone 14226-3; Millipore, 04-1540; 1:100 dilution), by following the co-extraction protocol described by (Bizard et al ...
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bioRxiv - Cell Biology 2021Quote: ... the tissue was dissolved in 3 ml freshly prepared primary dissociation buffer (collagenase 1 mg/ml, Sigma C6885, in DMEM w/o phenol red, Gibco) and passed through a cut p1000 pipet tip ten times or until smooth passing ...
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bioRxiv - Neuroscience 2021Quote: ... sample from the overnight cleavage step was concentrated to ∼5 mg/ml and injected into the Superose 6 Increase column equilibrated in SEC buffer with 1 mM DEET (Sigma Aldrich, CAS#134-62-3). Peak fractions were concentrated to ∼5 mg/ml and used immediately for grid preparation.
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bioRxiv - Biochemistry 2020Quote: ... serial 10 µm cryosections were collected starting at the superior edge of the optic nerve head on pre-labeled 1×3 mm glass slides coated with 10% poly-L-lysine (Sigma Aldrich, St. Louis, MO, USA) and maintained at 37° during sectioning ...
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bioRxiv - Genetics 2021Quote: ... followed by blocking with milk or bovine serum albumin (BSA) and antibody labelling with specific antibodies to FLAG (Sigma F1804; 1:5000 in 3 % milk) or RPS-6 (Abcam ab70227 ...
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bioRxiv - Microbiology 2023Quote: ... Parasites were permeabilized with a 0.1% Triton-X 100 solution (JT Baker, Phillipsburg, New Jersey, USA) for 10 minutes and blocked with 3% BSA (Sigma-Aldrich, St. Louis, Missouri, USA) in PBS for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... cenocepacia in a 3:1 ratio on SOB plates containing 100 μg/mL of 2,6-diaminopimelic acid (DAP, Sigma-Aldrich, St. Louis, MO, United States) for 2 days at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... a separate cohort of tissue sections were pre-incubated for 30 minutes at 37◦C with either the NOX1 inhibitor ML171 (1 µM; CAS 6631-94-3, Sigma Aldrich; St. Louis, MO, USA) or the NOX2 inhibitor GSK (50 µM ...
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bioRxiv - Molecular Biology 2023Quote: ... preadipocytes were induced to differentiate using induction medium (DIFF1) containing growth medium supplemented with 0.5 mM 3-Isobutyl-1-Methylxathine (IBMX) (Sigma-Aldrich, St. Louis, MO, USA I5879), 1 μM Dexamethasone (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... the brain sections were rinsed in phosphate buffer (PB) and incubated 1 h in a solution containing equal volumes of 3% potassium ferrocyanide (Sigma-Aldrich, Ontario, Canada, cat# P9387) and 4% osmium tetroxide (EMS ...
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bioRxiv - Plant Biology 2022Quote: ... Pellets were resuspended in 620µl of nuclei lysis buffer (50mM Tris-HCl, 10mM EDTA, 1% SDS, 2mM PMSF, 2µg/ml protease inhibitor (Sigma-Aldrich, catalog No. 200-664-3). The suspensions were sonicated for 20min with the Bioruptor® Sonication System (Diagenode ...
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bioRxiv - Cell Biology 2023Quote: ... cells were seeded in microslide channels at a final concentration of ∼5×105 cells/ml in 120 µL cell culture media containing 40 μM BrdU:BrdC mix (3:1, Cat.# B5002, Sigma and Cat.# 284555, Santa Cruz Biotec) and grown for 17-24 h ...
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bioRxiv - Biophysics 2024Quote: Adhesion of acrylic-based photoresists to the glass substrate surface was enhanced by functionalizing the 30 mm glass coverslips with 3-(trimethoxysilyl)propyl methacrylate (Sigma Aldrich; 1 mM in ethanol) for 5 minutes ...
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bioRxiv - Biophysics 2024Quote: ... 1 eq of nonlabelled α-Syn monomer was buffered exchange to the NMR buffer using a 3 kDa centrifuge filter (Millipore Sigma, St. Louis, MO) and directly added to the PDZ ...
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bioRxiv - Biochemistry 2022Quote: ... tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (34 μM, Sigma, 678937), copper(II ...
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bioRxiv - Biochemistry 2022Quote: ... tris(1-benzyl-1H-1,2,3-triazol-4-yl-)methylamine (TBTA, 100 μM, Sigma-Aldrich), and CuSO4 (1 mM ...
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bioRxiv - Cell Biology 2022Quote: ... 0.1 mM Tris-[(1-benzyl-1H-1,2,3-triazol-4-yl)-methyl]-amin (Sigma Aldrich), and 1 mM sodium ascorbate ...
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bioRxiv - Molecular Biology 2023Quote: ... tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, 678937) and palmitoyl coenzyme A (≥ 90% ...
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bioRxiv - Microbiology 2023Quote: ... 62.5 µM tris[(1-benzyl-1H-1,2,3-triazol-4- yl)methyl]amine (TBTA) (Sigma), 1.25 mM CuSO4 ...
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bioRxiv - Biophysics 2021Quote: ... The peak fractions from size exclusion column were pooled and concentrated using 3 kD cutoff ultracentrifugal device (Millipore, USA). The EspC purity was checked on 12% SDS-PAGE and by mass spectrometry ...
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bioRxiv - Cancer Biology 2021Quote: ... 50,000 cells at ∼70% viability were spun down and incubated 3 min at 4°C in 25µl of a detergent buffer containing 0.2% Igepal CA-630 (Sigma, #I8896), 0.2% Tween 20 (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... A Hybond N+ membrane (GE lifesciences, 45000854) and 6 sheets of 3 mm Whatman cellulose paper (Sigma Aldrich, WHA3030861) were pre-soaked for 5 minutes in room temperature 0.5X TBE ...
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bioRxiv - Systems Biology 2020Quote: ... The resultant pellets were resuspended in 3% acetonitrile + 0.1% trifluoroacetic acid and peptide quantification performed using the Direct Detect system (Merck Millipore). Protein samples were normalized then vacuum concentrated in preparation for mass spectrometry.
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bioRxiv - Cell Biology 2020Quote: ... Retinal organoids (n=3-4) for each time point were homogenized using a Dounce Tissue Grinder (Sigma-Aldrich, UK) and processed using SYBR™ Green Fast Advanced Cells-to-CT ™ Kit (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: HBEC6-KT and Calu-3 cells were lysed using RIPA Lysis Buffer containing Protease Inhibitor Cocktail powder (Sigma-Aldrich) for 60-90 minutes at 4°C on a rocker ...
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bioRxiv - Immunology 2021Quote: ... 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium (BCIP /NBT) liquid substrates for AP-enzyme (SIGMA) were added for overnight incubation at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Wells were washed and spots detected by adding a filtered solution of 0.2mg/mL AEC (3-amino-9-ethylcarbazole; Sigma) in 0.1M sodium acetate buffer [pH 5.0] and 0.05% hydrogen peroxide (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... Abcam-17785-1-AP) and PSD-95 ((2.5 μg/ml diluted) Abcam-ab12093) using desired concentrations in PBS containing 3% BSA (Sigma), 0.3% Sodium azide (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed 3 times and secondary staining was done in permeabilization buffer supplemented with 5% Donkey serum (Sigma) for 1 hour ...