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Citations for Addgene :
5101 - 5150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: AID12* and AID123* or EvolvR (Addgene #83260) were kind gifts from Rahul Kohli (University of Pennsylvania ...
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bioRxiv - Genetics 2024Quote: pattB-Actin5C-BE2(pActin5c-CD-dCas9m4-UDI-attB) was obtained from Addgene# 104879 (Marr and Potter ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and psPAX2 (gift from Didier Trono, Addgene plasmid # 12260), into HEK293T cells with a 1:1:1 stoichiometry ...
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bioRxiv - Cell Biology 2024Quote: ... mEmerald-γ-tubulin was from Addgene #54105 ...
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bioRxiv - Cancer Biology 2024Quote: ... pLenti CMV rtTA3 Hygro (w785-1) (Addgene: 26730), pCW57.1-4EBP1_4xAla (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: ... pCW57.1-4EBP1_4xAla (Addgene: 38240).
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bioRxiv - Cancer Biology 2024Quote: 5’ UTR elements or part of the GAPDH gene body102 were cloned into the dual luciferase assay construct (Addgene:45642). 1.5 million cells/6cm plate were transfected with 2μg of the construct using lipofectamine 2000 at a ratio of 3:1 lipofectamine to μg DNA ...
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bioRxiv - Cancer Biology 2024Quote: ... 293T cells were plated in 10cm plates and transfected using lipofectamine 2000 with 6μg lentiviral pCW57.1-4EBP1_4xAla (Addgene: 38240), 1.5μg pMD2G and 4.5μg psPax2 ...
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bioRxiv - Cell Biology 2024Quote: ... and pLenti-CRISPRvs-puro (Plasmid 98291, Addgene; target = GCAGCACCGAAGCTTCGTTG-AGG). Lentivirus was generated in HEK293 cells and placed directly on fibroblasts ...
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bioRxiv - Cell Biology 2024Quote: ... two lentiviral plasmids targeting Myh9 were constructed: pLenti-CRISPRvs-puro (Plasmid 98290, Addgene; target = ACCCTGCATCATGCTCCGGT-AGG) and pLenti-CRISPRvs-puro (Plasmid 98291 ...
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bioRxiv - Cell Biology 2024Quote: ... RPE1-iCas9 cells were transfected with Cas9-2A-EGFP (pX458; Addgene #48138) containing a gRNA against CSB ...
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bioRxiv - Neuroscience 2024Quote: ... and were a gift from Cheryl Arrowsmith (Addgene plasmid #111741; http://n2t.net/addgene:111741; RRID:Addgene_111741 and Addgene plasmid #111730; http://n2t.net/addgene:111730; RRID:Addgene_111730) [35].
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bioRxiv - Neuroscience 2024Quote: ... The viruses used for this study were jGCaMP8s (AAV9-pGP-syn-FLEX-jGCaMP8s-WPRE titer: > 1x 1013, Addgene #162377) and GRABACh3.0 (AAV9-hSyn-DIO-GRABACh3.0 titer ...
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bioRxiv - Neuroscience 2024Quote: ... (gift from Marius Wernig; Addgene plasmid # 52047).91 The NGN2 cassette was replaced with the tau scFvs and their respective mPEST ...
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bioRxiv - Neuroscience 2024Quote: ... FUW-M2rtTA (rtTA) (a gift from Dr. Rudolf Jaenisch, Addgene plasmid # 20342).92 Lentiviruses were produced in 293FT cells (ThermoFisher ...
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bioRxiv - Biophysics 2024Quote: ... and inserted into the SspI site of the 2B-T modified pET vector (Addgene plasmid #29666) using the ligation-independent cloning protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and pMD2.G (Addgene # 12259), both produced by Didier Trono ...
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bioRxiv - Cancer Biology 2024Quote: ... lentiCRISPR v2 hBAX (Addgene, 129580) and/or LentiCRISPR v2 hBAK1 (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: ... and/or LentiCRISPR v2 hBAK1 (Addgene, 129579) plasmids using Lipofectamine 2000 ...
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bioRxiv - Cancer Biology 2024Quote: Tet-pLKO-puro (Addgene #21915) was used to express doxycycline-inducible shRNA ...
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bioRxiv - Neuroscience 2024Quote: ... pcDNA-SADB19G (32633) and pBOB-synP-HTB (30195) were acquired from Addgene. The original pSADdeltaG-F3 plasmid was modified by subcloning the encoding sequence of mCherry protein between NheI and SacII restriction sites ...
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bioRxiv - Neuroscience 2024Quote: ... adult male and female wildtype mice received 500 nL of AAV9-CaMKIIa-GCaMP6s-WPRE-SV40 (Addgene, Watertown, MA) in the right parabrachial nucleus (PBN ...
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bioRxiv - Neuroscience 2024Quote: ... iPSCs were transfected with pC13N-dCas9-BFP-KRAB and TALENS targeting the human CLYBL intragenic safe harbor locus (between exons 2 and 3) (pZT-C13-R1 and pZT-C13-L1, Addgene #62196, #62197) using DNA In-Stem (VitaScientific) ...
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bioRxiv - Neuroscience 2024Quote: ... or retrograde serotype AAV-CAG-tdTomato (gift from Edward Boyden; Addgene Addgene viral prep # 59462-AAVrg) were injected via a glass capillary nanoinjector (Neurostar ...
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bioRxiv - Genetics 2024Quote: ... Synthetic oligonucleotides incorporating the gRNA sequences were ligated into pX330 plasmid (Addgene, Watertown, MA 02472) to co-express Cas9 and gRNA ...
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bioRxiv - Genetics 2024Quote: ... individual single and 3-plex crRNA constructs were cloned into the human U6 promoter-driven expression vector pRG212 (Addgene 149722, originally from 29). Library1 ...
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bioRxiv - Genetics 2024Quote: We began with an intronized Cas9 variant known as zCas9i (pAGM55285, which was a gift from Sylvestre Marillonnet, Addgene #153212)116 ...
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bioRxiv - Genetics 2024Quote: To assemble the gRNA cassette we used the shuttle vectors from Stuttmann et al (pDGE332, pDGE333, pDGE335, and pDGE336, which were a gift from Johannes Stuttmann, Addgene #153241, 153242, 153243, and 153244)118 ...
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bioRxiv - Genetics 2024Quote: ... the open reading frame was cloned into a piggyBac vector backbone (Addgene #133568) and expressed from a CAG promoter ...
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bioRxiv - Genetics 2024Quote: ... The denAsCas12a open reading frame was PCR amplified from pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776) (Addgene plasmid # 107943, from 31). AsCas12a variants described were generated by using the denAsCas12a open reading frame as starting template and introducing the specific mutations encoded in overhangs on PCR primers that serve as junctions of Gibson assembly reactions ...
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bioRxiv - Cell Biology 2024Quote: ... GFP–P4M-SidMx2 (51472; Addgene) was amplified by PCR and subcloned into pMRXIP.
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bioRxiv - Cell Biology 2024Quote: ... and pMRXIB (harboring a blasticidin-resistant marker) (Morita et al., 2018) together with enhanced GFP or mRuby3 (codon-optimized, Addgene #74252). STX17 fragments and their point mutations were generated by a standard PCR method or PCR-mediated site-directed mutagenesis.
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bioRxiv - Evolutionary Biology 2024Quote: ... pDR274 (plasmid # 42250; Addgene) was digested with the BsaI restriction enzyme ...
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bioRxiv - Developmental Biology 2024Quote: ... and PMD2G (Addgene #12259). Seventy-two hours after transfection ...
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bioRxiv - Developmental Biology 2024Quote: ... we cloned Hdac3 sgRNA into lentiCRISPR v2 vector (Addgene #52961; Watertown, MA). Seventy-percent confluent Lenti-XTM 293T cells (Takara Bio USA ...
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bioRxiv - Developmental Biology 2024Quote: ... PsPAX2 (Addgene #12260) and PMD2G (Addgene #12259) ...
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bioRxiv - Cancer Biology 2024Quote: ... Drp1 coding sequence were subcloned into pLenti-CMV-MCS-GFP-SV-puro (Addgene, 73582) with a N-terminal GFP tag and sequenced to confirm successful cloning of Drp1(−/17 ...
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bioRxiv - Cancer Biology 2024Quote: ... mitochondria were labelled in cells by transduction with pLV-mitoDsRed virus (Addgene, Plasmid 44386) harvested post expression in 293-FT cells and lentiviral particle production ...
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bioRxiv - Cell Biology 2024Quote: ... The sgRNA sequences (Extended Data Table 2) were inserted into the pDD162 vector (Addgene #47549) by linearizing the vector with 15 base pairs (bp ...
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bioRxiv - Plant Biology 2024Quote: ... The assembled plasmid pDL was deposited at Addgene (Addgene no ...
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bioRxiv - Physiology 2024Quote: ... We also injected AAV-phSyn1(s)-FLEX-tdTomato-T2A-SypGFP-WPRE (Addgene 51509-AAV1) into the DMV (500 nl of 4 x 1012 pfu/ml per site ...
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bioRxiv - Plant Biology 2024Quote: ... mScarletI sequence derived from Addgene 98839 ...
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bioRxiv - Plant Biology 2024Quote: ... directly followed by the mTFP sequence (Addgene 55503), 3XGGGS linker (GGT GGA GGC GGT TCA GGC GGA GGT GGC TCT GGC GGT GGC GGA TCG) ...
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bioRxiv - Physiology 2024Quote: ... ChATCre mice received an AAV.Php.eB-flex-tdTomato (titer; 1.9×1013 pfu/ml, Addgene 28306-PHP.eB) injection into the liver ...
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bioRxiv - Cell Biology 2024Quote: ... The pInducer20 was a kind gift from Stephen Elledge (Addgene #44012). The respective transgenes were first sub-cloned by TOPO cloning into a Gateway™ entry vector (pENTR™/D-TOPO™ ...
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bioRxiv - Cell Biology 2024Quote: ... and 3.5 µg pMD2.G (Addgene # 12259), encoding for the viral envelope components were gently mixed with H2O to a total volume of 597 µl ...
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bioRxiv - Cell Biology 2024Quote: ... The mEGFP-C1 plasmid was a kind gift from Michael Davidson (Addgene plasmid #54759).
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bioRxiv - Developmental Biology 2024Quote: ... mRor2 (Addgene, #22613), single or in combinations (totalling to 0.2μg for HEK293 cells and 0.6μg for frontonasal mass cultures) ...
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bioRxiv - Cell Biology 2024Quote: ... The sgRNA spacers were cloned into pSpCas9(BB)-2A-Puro (PX459) V2.0 plasmid (Addgene, cat. #62988) as described previously [91] ...
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bioRxiv - Neuroscience 2024Quote: ... pGP-AAVrg-hSyn-mCherry (7× 1012GC/ml; Addgene) was infused into the NAc (A/P ...