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Citations for Addgene :
401 - 450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... MSP1E3D1(-) (a gift from Stephen Sligar, Addgene plasmid #20066 ...
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bioRxiv - Biochemistry 2024Quote: ... The cDNA of APLF full length and APLFΔFHA and APLFΔAD were amplified with respective primer pairs (Table S1) and subcloned into Flag-HA-pCDNA3.1 (Addgene #52535) (Horn et al. ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... or AAV5-hSyn-DIO-GFP virus (Addgene Cat. No. 50457-AAV5, 7x1012vg/mL). The viral injection procedures were executed as described in [32] ...
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Locomotion-dependent auditory gating to the parietal cortex mediates flexible multisensory decisionsbioRxiv - Animal Behavior and Cognition 2024Quote: ... we bilaterally injected retrograde AAV expressing Cre-recombinase (AAVrg-hSyn-Cre-WPRE-hGH, Addgene) into the right PPC or bilateral STR (0.5 μl per each site ...
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Locomotion-dependent auditory gating to the parietal cortex mediates flexible multisensory decisionsbioRxiv - Animal Behavior and Cognition 2024Quote: ... and injected ~0.5 μl of AAV-hSyn-ChrimsonR-tdTomato (Addgene) into the right M2 at two anterior-posterior (AP ...
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Locomotion-dependent auditory gating to the parietal cortex mediates flexible multisensory decisionsbioRxiv - Animal Behavior and Cognition 2024Quote: ... we injected 0.5 μl of retrograde AAV expressing tdTomato (AAVrg-CAG-tdTomato, Addgene) and 0.5 μl of retrograde AAV expressing GFP (AAVrg-CAG-GFP ...
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Locomotion-dependent auditory gating to the parietal cortex mediates flexible multisensory decisionsbioRxiv - Animal Behavior and Cognition 2024Quote: ... we injected ~0.5 μl of retrograde AAV expressing Cre-recombinase (AAVrg-hSyn-Cre, Addgene) into the right AC (bregma −2.5mm ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Mice were unilaterally injected using a Nanoject II programmable nanoliter injector with 600 nL/hemisphere with AAV5-hSyn-dLight1.2 (Addgene Cat. #111068-AAV5) [33] into the NAc core using stereotaxic bregma-based coordinates ...
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Locomotion-dependent auditory gating to the parietal cortex mediates flexible multisensory decisionsbioRxiv - Animal Behavior and Cognition 2024Quote: ... and 0.5 μl of retrograde AAV expressing GFP (AAVrg-CAG-GFP, Addgene) into the target areas respectively ...
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bioRxiv - Cancer Biology 2024Quote: ... The following plasmid was used: pCMV-Sport6-CD36-pHluorin (Plasmid#130901, Addgene). siRNA oligos were transfected into cells using Lipofectamine RNAiMax Reagent according to the manufacturer’s protocol (Invitrogen) ...
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bioRxiv - Cancer Biology 2024Quote: The Tet-pLKO-puro lentivirus vector (plasmid#21915) and the control shRNA lentivirus vector (pLKO-Tet-On-shRNA-Control, plasmid#398398) were purchased from Addgene (Watertown, USA). The construction of the two shRNA lentivirus vectors targeting the human β-catenin was performed following the Tet-pLKO Manual given by Addgene (plasmid#21915) ...
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Locomotion-dependent auditory gating to the parietal cortex mediates flexible multisensory decisionsbioRxiv - Animal Behavior and Cognition 2024Quote: ... we injected ~0.5 μl of retrograde AAV expressing GCaMP6s (AAVrg-hSyn1-GCaMP6s-P2A-nls-dTomato, Addgene) into the right STR (bregma −1.5mm ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... heterozygous Adora2a-Cre mouse neonates were injected bilaterally with the Cre-recombinase-dependent viral vectors AAV5-hSyn- DIO-hM4D-mCherry virus (Addgene Cat ...
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Locomotion-dependent auditory gating to the parietal cortex mediates flexible multisensory decisionsbioRxiv - Animal Behavior and Cognition 2024Quote: ... we injected ~0.5 μl of retrograde AAV expressing GCaMP6s (AAVrg-hSyn1-GCaMP6s-P2A-nls-dTomato, Addgene) into the right PPC (bregma −1.95mm ...
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bioRxiv - Cell Biology 2024Quote: ... N-terminal FLAG-tagged CAPS was created by recombining the human CAPS gene (CAPS/pENTR; DNAsu HscD00514950) into pEZFLAG (Addgene#18700; Guo et al., 2008) using Gateway cloning ...
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bioRxiv - Pathology 2024Quote: ... pR26 CAG/GFP Asc was a gift from Ralf Kuehn (Addgene plasmid # 74285). Subsequently ...
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bioRxiv - Cancer Biology 2024Quote: sgRNAs were ordered as oligonucleotides from Sigma-Aldrich and inserted into the PX330 CRISPR vector (Addgene 42230) by digestion with BbsI ...
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bioRxiv - Cancer Biology 2024Quote: ... pLenti-Lifeact-tdTomato (RRID: Addgene_64048)
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bioRxiv - Cancer Biology 2024Quote: ... pCDH-EF1a-mCherry-EGFP-LC3B (RRID: Addgene_170446); pLenti-Lifeact-tdTomato (RRID ...
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bioRxiv - Cell Biology 2024Quote: ... The ER-Halo and the ER-GCaMP6-150 (Addgene #86918) under a CamKII promoter were both restriction digested with AgeI and the HALO protein was ligated with Quick Ligase (NEB ...
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bioRxiv - Plant Biology 2024Quote: ... digested vector pKS diaCas9_sgRNA (Addgene #74923). The homology donor plasmid was constructed via Gibson assembly (ThermoFisher GeneArt ...
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bioRxiv - Neuroscience 2024Quote: ... V2.0 was a gift from Feng Zhang (Broad Institute, Boston, USA) (Addgene plasmid # 62988; http://n2t.net/addgene:62988; RRID:Addgene_62988). Transient transfection of PX459 into OPs was conducted using Lipofectamine 3000 (Thermo Fisher) ...
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bioRxiv - Plant Biology 2024Quote: ... TurboID was amplified from Addgene plasmid #127350 (Mair et al. ...
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bioRxiv - Neuroscience 2024Quote: ... to express the genetically encoded calcium indicator GCaMP under the pan-neuronal synapsin promoter (AAV1-Syn-jGCaMP7f- WPRE, AddGene, viral prep 104488-AAV1, titer 2x1012). The needle was left in place for 10 min to allow for virus diffusion.
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bioRxiv - Neuroscience 2024Quote: ... and pAAV-mDlx-GFP-Fishell-1 (a gift from Gordon Fishell, Addgene plasmid # 83900; http://n2t.net/addgene:83900; RRID:Addgene_83900) by restriction subcloning ...
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bioRxiv - Neuroscience 2024Quote: ... and pAAV-mDlx-GFP-Fishell-1 (a gift from Gordon Fishell, Addgene plasmid # 83900 ...
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bioRxiv - Neuroscience 2024Quote: pAAV-mDlx-eGFP-ATG5 was generated from pEGFP-C1-hApg5 (a gift from Noboru Mizushima, Addgene plasmid # 22952) and pAAV-mDlx-GFP-Fishell-1 (a gift from Gordon Fishell ...
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bioRxiv - Neuroscience 2024Quote: ... and pAAV2/rh10 (Addgene plasmid # 112866 ...
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bioRxiv - Physiology 2024Quote: ... (1.3 × 1011 plaque-forming units per mouse, ((107787-AAV8, Addgene, Watertown, MA)) ...
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bioRxiv - Physiology 2024Quote: ... 200 ng pDisplay-gGRAB5HT2m-IRES-mCherryCAAX (Addgene, #208710), 200 ng pcDNA3-5-HT2A-P2A-GCaMP8m ...
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bioRxiv - Physiology 2024Quote: ... or 200 ng pDisplay-ATP1.0-IRES-mCherryCAAX (Addgene, #167582) was transfected to HEK293T cells in 24-well plates ...
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bioRxiv - Neuroscience 2024Quote: ... and pAAV2/rh10 (Addgene plasmid # 112866; http://n2t.net/addgene:112866; RRID:Addgene_112866) were a gift from James M ...
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bioRxiv - Neuroscience 2024Quote: Calcium indicator GCaMP6f (AAV1-Synapsin-GCaMP6f-WPRE-SV40, Addgene 100837-AAV1; 6.52 ×1012GC/ml) was injected using the same procedure at 4.5-5 months after injection of AAV-GFAP-htTau or AAV-GFAP-Control ...
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bioRxiv - Neuroscience 2024Quote: ... The plasmids pAdDeltaF6 (Addgene plasmid #112867; http://n2t.net/addgene:112867; RRID:Addgene_112867) and pAAV2/rh10 (Addgene plasmid # 112866 ...
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bioRxiv - Neuroscience 2024Quote: The pCAGGS-IRES-TdTomato construct was obtained from Addgene (#83029). pCAGGS-IRES- Cre-TdTomato was generated in the lab ...
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bioRxiv - Neuroscience 2024Quote: ... The mCherry plasmid was obtained from Addgene (#84329) (van Unen et al. ...
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bioRxiv - Neuroscience 2024Quote: ... To target astrocytes with chemogenetics we injected 0.60 µl bilaterally of the AAV5-GFAP-hM3D(Gq)-mCherry (Addgene 50478) (viral titer 2×1013 vg per ml ...
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bioRxiv - Neuroscience 2024Quote: ... was used to inject 300 nl of either a non-specific GtACR2 (pAAV1-CamKIIa-stGtACR2-FusionRed, Addgene #105669), or a Cre-dependent GtACR2 (pAAV1-hSyn1-SIO-stGtACR2-FusionRed ...
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bioRxiv - Physiology 2024Quote: ... or pcDNA3.1-PPARgamma2 (#78768, Addgene, Watertown, MA, USA) plasmids using Lipofectamine 3000 (ThermoFisher Scientific) ...
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bioRxiv - Physiology 2024Quote: ... were obtained from Addgene. pAAV7-DJ-CMV-TeNT-P2A-GFP was prepared and obtained through the Stanford Viral Core ...
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bioRxiv - Physiology 2024Quote: pAAV-hSyn-mCherry (Addgene #114472-AAV8) and pAAV-hSyn-hM3D(Gq)-mCherry (Addgene #50474-AAV8 ...
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bioRxiv - Physiology 2024Quote: ... and pAAV-hSyn-hM3D(Gq)-mCherry (Addgene #50474-AAV8) were obtained from Addgene ...
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bioRxiv - Physiology 2024Quote: ... Repair templates in pDD282 (Addgene 66823) (forward primer for upstream arm ...
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bioRxiv - Physiology 2024Quote: AAV9 virus expressing jGCaMP8m under a CAG promoter was obtained from Addgene (162381-AAV9). For injections ...
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bioRxiv - Plant Biology 2024Quote: ... together with a fragment containing the nucleotides encoding for the Pip1 pro-domain but lacking the signal peptide (pJK110; Supplemental Table S4) were combined in all 64 possible combinations with pICH41264 (Addgene #4799; Weber et al., 2011) in a BpiI Golden Gate reaction (Supplemental Table S4) ...
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bioRxiv - Synthetic Biology 2024Quote: ... The plasmid backbone pAM552G (35, 47) (Addgene accession #154131) was used for testing ribosome variants from RISE in vivo (see below) ...
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bioRxiv - Synthetic Biology 2024Quote: ... pJL1sfGFP (encoding the reporter sfGFP under a T7 promoter, Addgene accession #102634) and pRDV3xFLAG-TolA-HH (encoding the 3xFLAG tag for ribosome selection under a T7 promoter ...
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bioRxiv - Synthetic Biology 2024Quote: A plasmid containing GCaMP6m was obtained from Addgene (36) and gblocks were obtained from IDT ...
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bioRxiv - Neuroscience 2024Quote: ... Virus pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 (Addgene viral prep #1120050-AAV5) was injected into layer 2/3 of the primary somatosensory barrel (S1BF ...
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bioRxiv - Neuroscience 2024Quote: ... To monitor astrocytic Ca2+ signaling we used the genetically encoded Ca2+ indicator (GECI) AAV5-gfaABC1D-cyto-GCaMP6f (Addgene 52925) (viral injections of 0.6 µl ...