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Citations for Addgene :
401 - 450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: The coding sequence of APEX2 was PCR-amplified from APEX2 plasmid (a gift from Alice Ting, Addgene plasmid #72480, [86]) using specific primers containing restriction sites NotI (5’ ...
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bioRxiv - Cell Biology 2024Quote: ... a gift from Andrew Fire (Addgene plasmid #59936). For further details on targeting of atn-1 with Cas9 ...
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bioRxiv - Molecular Biology 2024Quote: ... plasmid pKAN-PCUP1-9myc-AID*(N) from Addgene (Morawska and Ulrich, 2013), and plasmid pGIK43 from Georgios Karras.
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bioRxiv - Molecular Biology 2024Quote: ... mCherry-LaminB1-10 plasmid was a gift from Michael Davidson (Addgene plasmid #55069 ...
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bioRxiv - Molecular Biology 2024Quote: ... Coding sequence for blasticidin resistance was amplified from pCRIS-PITCHv2_BSD_dTAG plasmid (Addgene, #9179) using primers BSD_fwd and BSD_rev ...
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bioRxiv - Molecular Biology 2024Quote: ... The expression plasmid encoding the hDicer_ΔHEL variant was purchased from Addgene (#51366) [39] ...
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bioRxiv - Molecular Biology 2024Quote: ... mCherry-LaminB1-10 plasmid was a gift from Michael Davidson (Addgene plasmid #55069; http://n2t.net/addgene:55069; RRID:Addgene_55069).
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bioRxiv - Neuroscience 2024Quote: ... pAAV.Syn.GCaMP6f.WPRE.SV40 was a gift from Douglas Kim & GENIE Project (Addgene viral prep # 100837-AAV9; http://n2t.net/addgene:100837; RRID:Addgene_100837), pAAV.CAG.GCaMP6f.WPRE.SV40 was a gift from Douglas Kim & GENIE Project (Addgene viral prep # 100836-AAV1 ...
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bioRxiv - Neuroscience 2024Quote: ... CAG::DIO-LR-Voltron2-TS-ER2-p2a-LR-CheRiff-TS-eYFP-ER2 (Addgene: #203229), and co-expressed it with CAG::Cre (Addgene ...
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bioRxiv - Molecular Biology 2024Quote: ... Lentiviral constructs expressing TRMT1-WT or TRMT1-Q530N were generated by T5 Exonuclease DNA Assembly (TEDA) of PCR fragments into pLenti-CMV-GFP-Blast (Addgene 17445) (Xia et al ...
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bioRxiv - Microbiology 2024Quote: ... Bru-GFP ΔENV was generated as previously described (63) with pmD2.G (Addgene).
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bioRxiv - Neuroscience 2024Quote: ... The he1.1:YFP cassette (he1.1:YFP_F, he1.1:YFP_R, Table 4) was amplified from p3E_he1a:YFP (Addgene, plasmid #113879), and the polyA terminator (polyA_F ...
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bioRxiv - Neuroscience 2024Quote: ... polyA_R, Table 3) and plasmid backbone (Col1E_F, Col1E_R, Table 4) were amplified from pPRISM-Stop-cmlc2-eGFP (Addgene kit #1000000154; Wierson et al., 2020). The PCR-amplified fragments were assembled using NEBuilder HiFi DNA Assembly Cloning Kit (E5520S ...
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bioRxiv - Neuroscience 2024Quote: ... Gbait-hsp70-QF2-pA (Addgene plasmid #122563), which contains a GFP bait sequence for Cas9-mediated linearization of the donor plasmid (Kimura et al. ...
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bioRxiv - Neuroscience 2024Quote: ... We injected in that region 3x750nL of an adeno-associated virus (AAV) mix of AAV1.Syn.GCaMP (6m: Addgene 100841 or 7f ...
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bioRxiv - Neuroscience 2024Quote: ... The chimeric Gqi9 protein was ordered from Addgene (Plasmid No. 125711). This was then further modified using PCR and cloned into the pcDNA3.1(+ ...
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bioRxiv - Neuroscience 2024Quote: ... We injected in that region 3x750nL of an adeno-associated virus (AAV) mix of AAV1.Syn.GCaMP (6m: Addgene 100841 or 7f: Addgene 104488; dilution 1:10 ∼ 1x1013 GC/mL) and AAV1.Syn.Flex.Chrimson.tdTomato (UNC Vector Core ...
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bioRxiv - Neuroscience 2024Quote: ... we injected a mix of AAV1.Syn.jGCaMP7f (Addgene 104488; dilution 1:10 ∼ 1x1013 GC/mL) and AAV1.Syn.Flex.tdTomato (Addgene 28306 ...
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bioRxiv - Neuroscience 2024Quote: ... and AAV1.Syn.Flex.tdTomato (Addgene 28306 ...
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bioRxiv - Neuroscience 2024Quote: ... and 20% Cre-dependent GCaMP6f (AAV.CAG.Flex.GCaMP6f.WPRE.SV4089; Addgene, #100835). For SK2-KO mice ...
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bioRxiv - Neuroscience 2024Quote: All mice were injected bilaterally with AAV9-syn-FLEX-jGCaMP7s-WPRE (Addgene, 104491-AAV9) in the AIC at coordinates A/P ...
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bioRxiv - Neuroscience 2024Quote: ... ±2.25, D/V: −3.10 (100 nl/injection, 25 nl/min infusion rate) and AAVrg-Ef1a-mCherry-IRES-Cre (Addgene, 55632-AAVrg) in the DLS at coordinates A/P ...
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bioRxiv - Neuroscience 2024Quote: ... QF2 was amplified from Gbait-hsp70-QF2-pA (Addgene plasmid #122563 ...
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bioRxiv - Neuroscience 2024Quote: ... pT3TS-nCas9n template DNA (Addgene, plasmid #46757; Jao et al., 2013) was digested with XbaI (R0145S ...
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bioRxiv - Neuroscience 2024Quote: ... The resulting DNA was cloned into the pDR274 vector (Addgene, plasmid #42250; Hwang et al., 2013) following digestion of pDR274 with BsaI (R3733S ...
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bioRxiv - Genetics 2024Quote: ... The fragment was subcloned into pPD49.83 (a gift from Andrew Fire, Addgene plasmid # 1448 ...
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bioRxiv - Genetics 2024Quote: ... pHT101 (a gift from Casonya Johnson, Addgene plasmid # 61021; http://n2t.net/addgene:61021; RRID: Addgene_61021) using Pst1 and Age1 restriction sites ...
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bioRxiv - Genetics 2024Quote: ... pHT101 (a gift from Casonya Johnson, Addgene plasmid # 61021 ...
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bioRxiv - Genetics 2024Quote: ... The fragment was subcloned into pPD49.83 (a gift from Andrew Fire, Addgene plasmid # 1448; http://n2t.net/addgene:1448 ; RRID:Addgene_1448) using BamH1 and Nco1 restriction sites ...
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The haplolethal gene wupA of Drosophila exhibits potential as a target for an X-poisoning gene drivebioRxiv - Genetics 2024Quote: The design protocol of Port and Bullock (2016) was used to clone gRNA sequences into the pCFD5_w plasmid (Addgene #112645). Three separate plasmids were generated that targeted haplolethal genes on the X chromosome ...
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bioRxiv - Biochemistry 2024Quote: Biotinylated proteins were co-expressed with BirA (PET21a-BirA, Addgene #20857) in E ...
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bioRxiv - Developmental Biology 2024Quote: ... which was then cloned into pENTR-dTOPO (Thermo) for Gateway cloning into PB-TA-ERN (Knut Woltjen, Addgene #80474, (Kim et al., 2016)) to generate PB-TA-ERN-hTFAP2A(CR)-HA ...
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bioRxiv - Developmental Biology 2024Quote: ... The TFAP2A gRNA targeted site of human TFAP2A construct (gift of Robert Tjian, Addgene #12100, (Williams and Tjian, 1991)) was mutated using Q5 site directed mutagenesis kit (NEB ...
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bioRxiv - Neuroscience 2024Quote: ... and co-expressed it with CAG::Cre (Addgene: #13775) plasmid wt/wt = 30:1 for in utero electroporation ...
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bioRxiv - Neuroscience 2024Quote: ... AAV5-Syn-FLEX-rc [ChrimsonR-tdTomato] (FLEX-Chrimson, 1.20e+13 gc/mL, 250 nL, Addgene #62723-AAV5), AAVrg-hSyn-Cre-WPRE-hGH (retro-Cre ...
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bioRxiv - Neuroscience 2024Quote: ... AAVrg-hSyn-Cre-WPRE-hGH (retro-Cre, 2.10e+13 gc/mL, 80 nL, Addgene #105553-AAVrg), AAV1-Syn-Chronos-GFP (Chronos ...
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bioRxiv - Neuroscience 2024Quote: ... To optimize expression and dendritic membrane trafficking we designed the construct CAG::LR-Voltron2-TS-ER2-p2a-LR-CheRiff-TS-eYFP-ER2 (Addgene: #203228). In this construct ...
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bioRxiv - Neuroscience 2024Quote: ... Pulled injection pipettes were beveled and back-filled with mineral oil before being loaded with one or more of the following: AAV1-Syn-ChrimsonR-tdTomato (Chrimson, 2.10e+13 gc/mL, 250 nL, Addgene #59171-AAV1), AAV5-Syn-FLEX-rc [ChrimsonR-tdTomato] (FLEX-Chrimson ...
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bioRxiv - Neuroscience 2024Quote: The Ca2+ indicator jRGECO1a (Dana et al., 2016) was expressed in IC neurons through induction with AAV vectors (Addgene #100854-AAV1), which were stereotaxically delivered as follows ...
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bioRxiv - Neuroscience 2024Quote: ... AAV-syn-FLEX-jGCaMP7b-WPRE (FLEX-GCaMP7b, 1.90e+13 gc/mL, 250nl, Addgene #104493-AAV1), ssAAV-retro/2-hSyn1-mCherry_iCre-WPRE-hGHp(A ...
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bioRxiv - Neuroscience 2024Quote: ... AAV1-Syn-Chronos-GFP (Chronos, 2.90e+13 gc/mL, 250 nL, Addgene #59170-AAV1), AAV-syn-FLEX-jGCaMP7b-WPRE (FLEX-GCaMP7b ...
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bioRxiv - Neuroscience 2024Quote: ... p3E-mKate2-myc no-pA (Addgene, 80812), and pDESTtol2pACrymCherry (Addgene ...
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bioRxiv - Molecular Biology 2024Quote: ... the FRT-STOP-FRT cassette sequence was PCR amplified from pJFRC201-10xUAS-FRT-STOP-FRT-myr::smGFP-HA (#63166; Addgene). The coding sequence of mCD8::DQVD/A was PCR-amplified from the genomic DNA of Caspase-Sensitive/Insensitive-Gal4 [20] ...
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bioRxiv - Molecular Biology 2024Quote: ... the coding sequence of mNeonGreen was PCR-amplified from pAc5-V5::mNeonGreen [17] and ligated into the EcoRI-digested pQUAST (#24349, Addgene) vector using In-Fusion ...
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bioRxiv - Microbiology 2024Quote: ... pHelper and pAAV-2.5 capsid plasmids were used (Addgene and (16). For VLP production ...
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bioRxiv - Microbiology 2024Quote: ... psPAX2 and pmD2.G were used (Addgene). For HIV-1 ΔENV production ...
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bioRxiv - Genomics 2024Quote: ... we nucleofected five million cells with five µg of spCas9/sgRNA-expression plasmids (pX330-U6-Chimeric-BB-CBh-hSpCas9, Addgene #42230) by Nucleofector 2b ...
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bioRxiv - Genomics 2024Quote: ... and then cloned into the EagI and EcoRI sites of pJK03 (AddGene #173,490) in multiple ligation reactions (NEB T4 ligase) ...
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bioRxiv - Genomics 2024Quote: ... Basal promoter inserts were prepared by amplifying the Rho basal promoter and DsRed from the plasmid pJK01 (AddGene #173,489) using the forward primer MO566 and reverse primers that add 9bp multiplexing barcodes (mBC ...
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bioRxiv - Molecular Biology 2024Quote: ... the coding sequences of FLPo were PCR-amplified from pQUAST-FLPo (#24357, Addgene) and ligated into the XhoI-digested pUASz1.0 vector [45] using In-Fusion ...