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Citations for Addgene :
201 - 250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... cells were transduced with an inducible system Tet-pLKO-puro (21915, Addgene) encoding an shRNA targeting TARDBP sequence (TRCN0000016038 ...
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bioRxiv - Neuroscience 2024Quote: ... and pMD2.G (12259, Addgene) plasmids (both a gift from Dr ...
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bioRxiv - Neuroscience 2024Quote: ... peGFP-N1-FLAG (Addgene plasmid # 60360).
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bioRxiv - Systems Biology 2024Quote: ... and pMW#3 (Addgene #13350) destination vectors using LR Clonase (ThermoFisher #11791100) ...
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bioRxiv - Neuroscience 2024Quote: AAV serotype 5 OTp-hM3Dq-Myc (2.9 × 1012 gp/mL) (Corresponding plasmid: Addgene #184753)84
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bioRxiv - Neuroscience 2024Quote: ... The AAV serotype DJ hSyn-DIO-hM4Di-mCherry (6.9 × 1012 gp/mL) was generated by the Fukushima Medical University School of Medicine Viral Vector Core facility with the corresponding plasmid (Addgene #44362).
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bioRxiv - Neuroscience 2024Quote: AAV serotype 1 CaMK2a-EYFP (1.0 × 1013 gp/mL) (Addgene #105622)
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bioRxiv - Systems Biology 2024Quote: ... coli origin of replication and ampicillin resistance gene were PCR amplified from pCRISPRyl (Addgene #70007) (Schwartz et al. ...
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bioRxiv - Systems Biology 2024Quote: ... PpHIS4 gene was PCR amplified from pMJA089 (Addgene #128518) (Yang et al. ...
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bioRxiv - Systems Biology 2024Quote: ... PCR amplified constructs were inserted into mCherry-CRY2clust (Addgene #105624), using BsrGI/HpaI sites to obtain mCherry tagged JNK2α2 and SgrAI/HpaI sites to obtain mCherry tagged ASK1 constructs ...
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bioRxiv - Systems Biology 2024Quote: ... pPBbsr-JNKKTR-mCherry plasmid containing JNK activity reporter (JNK-KTR) was obtained from Addgene (#115493). mCherry in this plasmid was exchanged with iRFP713 (from Addgene #111510 ...
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bioRxiv - Systems Biology 2024Quote: ... ClopHensor plasmid was obtained from Addgene (#25938). JNK2α2 and ASK1 were amplified from pCDNA3 Flag Jnk2a2 (Addgene #13755 ...
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bioRxiv - Systems Biology 2024Quote: ... mCherry in this plasmid was exchanged with iRFP713 (from Addgene #111510) fluorophore using NotI and SalI enzymes ...
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bioRxiv - Systems Biology 2024Quote: ... JNK2α2 and ASK1 were amplified from pCDNA3 Flag Jnk2a2 (Addgene #13755) and pCMV6-Myc-MAP3K5 (ASK1 ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... 2 μg pMD2.G (Addgene #12259), and 36 μL Turbofect (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... into the BsmBI-digested pBPK1520 plasmid (BPK1520 was a gift from Keith Joung. Addgene#65777; http://n2t.net/addgene: 65777; RRID:Addgene_65777)138 ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli chromosome at the λ integration site by electroporating this plasmid into DH10B cells containing the helper plasmid pInt-ts (Addgene #66076) and selecting for kanamycin resistant colonies ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli DH10GFP (Addgene #109392), a strain constructed in the same way in the prior study of burden by Ceroni et al.21
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bioRxiv - Synthetic Biology 2024Quote: ... we inserted the constitutive GFP expression cassette cloned into pAH63 (Addgene #66073) into the E ...
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bioRxiv - Neuroscience 2024Quote: ... into the BsaI-digested pU6-peg-GG-acceptor (pU6-pegRNA-GG-acceptor was a gift from David Liu. Addgene #132777; http://n2t.net/addgene: 132777; RRID:Addgene_132777). Prime editing nicking guide plasmids (ngRNAs ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli BW25113 carrying pDY118A using a donor plasmid (pKAW086, Figure S3, Table S2, Addgene ID 217967) containing an editing cassette comprised of kanR flanked by regions of homology to the genome (Table S6) ...
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bioRxiv - Neuroscience 2024Quote: ... Plasmid based prime editing: 500 ng pCMV-PE2-GFP (a gift from David Liu, Addgene#132776)115 ...
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bioRxiv - Neuroscience 2024Quote: ... pegRNA plasmids for prime editing were cloned by ligating annealed oligonucleotide pairs (Supplemental table 5) into the BsaI-digested pU6-peg-GG-acceptor (pU6-pegRNA-GG-acceptor was a gift from David Liu. Addgene #132777 ...
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bioRxiv - Synthetic Biology 2024Quote: ... The editing cassette (Table S7) was amplified from pKAW066 (Figure S4, Table S2, Addgene ID 217968) using the manufacturer’s protocol for NEB Q5 Hot Start DNA Polymerase (M0494L ...
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bioRxiv - Synthetic Biology 2024Quote: ... and eleven were procured from Addgene (Watertown, MA, USA). One plasmid solution was taken from a transfection kit where the mixture contains two plasmids with the same vector backbone ...
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bioRxiv - Neuroscience 2024Quote: ... or AAVretro-Cre (pAAV-Ef1a-mCherry-IRES-Cre, 55632-AAVrg, 1.1-3.5 x 1012 viral genomes/ml, Addgene) into the ipsilateral posterior medial nucleus of the thalamus (POM) ...
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bioRxiv - Neuroscience 2024Quote: ... AAV2/5.CaMKII.tdTomato (Neurophotonics, 661-aav5) was used to label excitatory neurons and AAV9.CaMKII.GCaMP6s.WPRE.SV40 (Addgene, 107790) was employed to image excitatory neuronal calcium activity ...
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bioRxiv - Neuroscience 2024Quote: ... 2.3 nL of AAV1-CAG-tdTomato (59462-AAV1; Addgene) were injected twice ...
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bioRxiv - Synthetic Biology 2024Quote: ... pUS116 (6.6 kb) (AddGene #84578) is a Mycobacterium-E ...
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bioRxiv - Synthetic Biology 2024Quote: ... all plasmids deposited to Addgene were independently subjected to whole-plasmid sequence confirmation by Addgene.
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bioRxiv - Synthetic Biology 2024Quote: ... These parts were constructed by obtaining a template plasmid from Addgene, amplifying the desired fragment by PCR with primers that introduce the required SapI cut sites and overhangs ...
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bioRxiv - Synthetic Biology 2024Quote: ... pIB184-Km (Addgene plasmid # 90195) and pIB185 (Addgene plasmid # 90196 ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmids pIB166 (Addgene plasmid # 90189), pIB184-Km (Addgene plasmid # 90195 ...
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bioRxiv - Systems Biology 2024Quote: ... pcDNA3 VP4:VP2 (Addgene, 216787) was prepared by PCR cloning of amino acids 1–332 from pcDNA3 CVB3 into the BamHI–EcoRI sites of pcDNA3 ...
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bioRxiv - Systems Biology 2024Quote: ... 4.5 μg of psPAX2 (Addgene #12260), 1.5 μg of pMD2.G (Addgene #12259 ...
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bioRxiv - Synthetic Biology 2024Quote: ... and pIB185 (Addgene plasmid # 90196) were donated by Indranil Biswas ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV-mDlx-ChR2-mCherry-Fishell-3 was a gift from Gordon Fishell (Addgene plasmid # 83898; http://n2t.net/addgene:83898; RRID:Addgene_83898) 51 to express ArchT 67 fused to TS-EYFP-ER sequence that was cloned from a pcDNA3.1 CMV-ChRmine-TS-EYFP-ER plasmid gift of the Hegemann laboratory at the Humboldt Universität ...
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bioRxiv - Cancer Biology 2024Quote: ... The CDS’ were then used to replace the KRAB-dCas9-DHFR coding region in an inducible-expression lentiviral plasmid (RRID:Addgene_167935) by Gibson assembly (Vazyme ...
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bioRxiv - Cancer Biology 2024Quote: ... Lentiviral particles were generated by co-transfecting LentiGuide-sgRNA constructs and packaging constructs (psPAX2, RRID:Addgene_12260; and pMD2.G, RRID:Addgene_12259) to 293T cells ...
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bioRxiv - Cancer Biology 2024Quote: ... Lentiviral particles were generated by co-transfecting LentiGuide-sgRNA constructs and packaging constructs (psPAX2, RRID:Addgene_12260; and pMD2.G, RRID:Addgene_12259) to 293T cells ...
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bioRxiv - Neuroscience 2024Quote: ... Unilateral (right hemisphere) 250nl injections of dLight1.2 [AAV5-hSyn-dLight1.2] (Addgene) were stereotaxically targeted to the mPFC (AP ...
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bioRxiv - Cancer Biology 2024Quote: ... plasmid and the negative control pMSCV-Neo-GFP/Empty (Addgene #105505) were purchased on Addgene ...
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bioRxiv - Molecular Biology 2024Quote: ... and pMD2.G (all from Addgene) into HEK293FT cells using PEI transfection reagent ...
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bioRxiv - Neuroscience 2024Quote: ... Pups were injected unilaterally with 1 μl of AAV9-hSyn-DIO-ChrimsonR-mRuby2-ST (2 × 1012 gc ml−1; Addgene #105448) or 1 μl of AAV9-CAG-Flex-ArchT (2 × 1012 gc ml−1 ...
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bioRxiv - Neuroscience 2024Quote: ... itself a derivative of the Fire vector pPD49.2 (Addgene plasmid # 1686 ...
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bioRxiv - Biochemistry 2024Quote: Xenopus tropicalis TTLL10 (res. 105-570) was cloned into pCoofy28 (Addgene plasmid #44004) and bacmid was produced by transformation of DH10EMBacY (Geneva Biotech) ...
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bioRxiv - Cancer Biology 2024Quote: ... Lentiviruses were produced using a similar procedure with 293T cells using the psPAX2 plasmid (Addgene, 12260) instead of pUMVC.
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bioRxiv - Cancer Biology 2024Quote: ... 6 μg pMD2.G (Addgene, 12259), 4 μg pUMVC (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: ... 4 μg pUMVC (Addgene, 8449) and 18 μg transfer plasmid were mixed in 700 μL DMEM medium (Corning ...
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bioRxiv - Cancer Biology 2024Quote: ... The backbone vector for shRNAs was purchased from Addgene (pLKO.1_mCherry, 128073). We followed the shRNA construction protocol from the Genetic Perturbation Platform web portal (https://portals.broadinstitute.org/gpp/public/resources/protocols) ...