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Citations for Addgene :
301 - 350 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: IGI-P0492 pHR-dCas9-NLS-VPR-mCherry was a gift from Jacob Corn (Addgene plasmid # 102245 ...
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bioRxiv - Cell Biology 2024Quote: ... Tom Rapoport (Harvard University, Massachusetts) (Addgene plasmid #86683 and #86678 ...
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bioRxiv - Cell Biology 2024Quote: ... the full f-tractin-mCherry coding sequence (from C1-F-tractin-mCherry, Addgene #155218) was subcloned into the lentiviral expression vector pLVX-M-puro (Takara 632164) ...
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bioRxiv - Cell Biology 2024Quote: IGI-P0492 pHR-dCas9-NLS-VPR-mCherry was a gift from Jacob Corn (Addgene plasmid # 102245 ; http://n2t.net/addgene:102245 ; RRID:Addgene_102245).
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bioRxiv - Cell Biology 2024Quote: ... Erik Snapp (Albert Einstein College of Medicine, present Howard Hughes Medical Institute Janelia Research Campus, Virginia) (Addgene plasmid # 68072), mCherry-CLIMP-63 ...
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bioRxiv - Cancer Biology 2024Quote: ... The hU6-ngRNA1-hU6-pegRNA2-EF1-α-Puro-2A-RFP-WPRE cassette was amplified from this backbone and ligated into pCMV-PEmax-P2A-hMLH1dn (Addgene plasmid 174828) between SgrDI (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... or NG-ABEmax (Addgene plasmid 124163). For prime editing experiments ...
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bioRxiv - Cancer Biology 2024Quote: ... 20ug of pCMV-mCherry-Cre (Addgene plasmid 27546) was electroporated into ∼30-40 million cells using the setting 1100V ...
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bioRxiv - Cancer Biology 2024Quote: ... 1ug of either SF3B1 sgRNA or Rosa26 sgRNA in pLKO.5 Puro-2A-GFP backbone was co-transfected with either 1ug of NG-ABE8e (Addgene plasmid 13849) or NG-ABEmax (Addgene plasmid 124163) ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.5ug of pegRNAs were co-transfected alone or with 0.5ug ngRNA with 1ug of either pCMV-PE2-P2A-GFP (Addgene plasmid 132776) or pCMV-PEmax-P2A-MLH1dn (Addgene plasmid 174828) ...
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bioRxiv - Cancer Biology 2024Quote: ... or pCMV-PEmax-P2A-MLH1dn (Addgene plasmid 174828). HEK293T ...
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bioRxiv - Cancer Biology 2024Quote: ... Cas9 HDR sgRNA sequence was designed using HDR Donor Designer (Horizon Discovery) and ligated into pSpCas9(BB)-2A-GFP (Addgene plasmid 48138) between BbsI (New England Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: ... All pegRNAs were ligated into the pU6-pegRNA-gg-acceptor vector (Addgene plasmid 132777) between BsaI (New England Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: ... GFP (35637) and p53R273H expression vectors (22934) were purchased from Addgene (Watertown, MA, USA). Each plasmid was co-transfected into HEK293T cells with psPAX2 (Addgene ...
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bioRxiv - Bioengineering 2024Quote: ... ELP48 was obtained from Addgene (Addgene Plasmid #68395). Sequencing of ELP48 showed it contained 44 ELP repeats ...
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bioRxiv - Bioengineering 2024Quote: ... ELP48 was obtained from Addgene (Addgene Plasmid #68395). Sequencing of ELP48 showed it contained 44 ELP repeats ...
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bioRxiv - Bioengineering 2024Quote: ... and pMD2.G (Addgene, catalog number 12259) into Lenti-X HEK293T ...
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bioRxiv - Bioengineering 2024Quote: ... pCMV-dR8.91 (Addgene, catalog number 12263), and pMD2.G (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: Plasmid encoding the sequence of the cleavage site for Caspase 3 (DEVD) and FLIP-GFP reporter (Addgene# 124428) was digested using the NheI_HF and XmaI (New England Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: ... the NINJ1 cDNA from pENTR223-NINJ1 (DNASU, HsCD00505254) and SLC7A11 cDNA from pENTR223-SLC7A11 (DNASU, HsCD00512940) were individually subcloned into pLVpuro-CMV-N-EGFP (Addgene, #122848) and pLVpuro-CMV-N-mCherry (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: ... pUltra-Chili (Addgene #48687), LentiV-Cas9-puro (Addgene #108100) ...
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bioRxiv - Cancer Biology 2024Quote: ... pUltra (Addgene #24129), pUltra-Chili (Addgene #48687) ...
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bioRxiv - Cancer Biology 2024Quote: ... and cloned into the pLenti_CMV_GFP_Blast (Addgene #17445) backbone ...
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bioRxiv - Cancer Biology 2024Quote: ... LGR2.1 (Addgene #108098) encoding the gRNA targeting the gene of interest ...
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bioRxiv - Bioengineering 2024Quote: The guides were integrated into a lentiviral guide expression plasmid (pcmb320, a gift from Michael Bassik (Addgene plasmid 89359). The knockout process was executed via lentiviral transduction ...
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bioRxiv - Bioengineering 2024Quote: ... or a doxycycline-inducible minimal CMV promoter (Addgene plasmid #26431). EGFP and tdTomato were co-expressed with Aqp1 and Oatp1b3 respectively using an internal ribosome entry site (IRES ...
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bioRxiv - Cancer Biology 2024Quote: GFP-SMARCA4 (Addgene, 65391) was used for the SMARCA4 mutation clone ...
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bioRxiv - Cancer Biology 2024Quote: ... were from Addgene (23484 ...
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bioRxiv - Bioengineering 2024Quote: ... 1.463μg of pMD2.G and 3.659μg of psPAX2 (Addgene, Watertown, MA) were transfected using JetPRIME (Polyplus ...
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bioRxiv - Neuroscience 2024Quote: ... and pAAV-hSyn-DIO-EGFP (Addgene) was injected at a volume of 150 nl at 3 sites ...
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bioRxiv - Molecular Biology 2024Quote: ... pUVMC (Addgene 8449)
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bioRxiv - Molecular Biology 2024Quote: ... a gift from Feng Zhang (Addgene plasmid #48138).
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bioRxiv - Molecular Biology 2024Quote: ... VSV-g (Addgene 8454), pUVMC (Addgene 8449)
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bioRxiv - Molecular Biology 2024Quote: ... B52 (Addgene 100708), VSV-g (Addgene 8454) ...
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bioRxiv - Molecular Biology 2024Quote: Plasmid vectors: pmEmerald-n1 (Addgene 53976), pBabe-puro (Addgene 10668) ...
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bioRxiv - Molecular Biology 2024Quote: ... pMcherry-c1 (Addgene 58476), B52 (Addgene 100708) ...
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bioRxiv - Molecular Biology 2024Quote: ... pBabe-puro (Addgene 10668), pCaggs (BCCM LMBP 2453) ...
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bioRxiv - Cancer Biology 2024Quote: ... 5′-GGCTGCCGTAGCGCGACGTG-3′) were cloned into pLentiCRISPRv2 (Addgene-52961). An NT gRNA (5′-GTATTACTGATATTGGTGGG-3′ ...
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bioRxiv - Cancer Biology 2024Quote: ... Efficient nuclease activity was confirmed using a reporter system (Addgene #67979, #67980; Supp. Fig. 1). We synthesized a custom single guide RNA (sgRNA ...
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bioRxiv - Cancer Biology 2024Quote: ... This library was cloned into pRC0162 (Addgene #195319) and sequence verified ...
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bioRxiv - Cancer Biology 2024Quote: ... S1 Table) were annealed and cloned into the pLKO.1 vector (RRID:Addgene_8453) (29 ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were obtained from Cell BioLabs and used to produce lentivirus by transfection with psPAX2 and pMD2.G (Addgene #12260, #12259) using Lipofectamine 3000.
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bioRxiv - Cancer Biology 2024Quote: ... The CRISPR-Cas9 cloning vector px459 (Addgene 62988; (28)) was a kind gift from Fred Dick ...
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bioRxiv - Cancer Biology 2024Quote: ... DHB-mVenus (CDK2 activity reporter) was a gift from Tobias Meyer & Sabrina Spencer (Addgene plasmid # 136461 ...
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bioRxiv - Cell Biology 2024Quote: ... pBOB-EF1-FastFUCCI-Puro was a gift from Kevin Brindle & Duncan Jodrell (Addgene plasmid # 86849; http://n2t.net/addgene:86849; RRID: Addgene_86849) (Koh et al ...
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bioRxiv - Cell Biology 2024Quote: The AAVS1 destination vector was assembled by PCR amplification and Gibson assembly from the pDTA-TK from Robert Benezra’s group (RRID:Addgene_22677) (Nam and Benezra 2009 ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lines for LysoIP were generated using constructs obtained from Addgene, details below ...
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bioRxiv - Cell Biology 2024Quote: ... The lentiviral vector lentiCRISPRv2 carrying both Cas9 enzyme and a gRNA transfected into HEK293T cells together with the packaging plasmids psPAX2 and pCMV-VSV-G (Addgene) at the ratio of 5:3:2 ...
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bioRxiv - Cell Biology 2024Quote: ... Each fragment was then cloned into the pScarlet-N1 plasmid (RRID:Addgene_128060) from Oskar Laur ...
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bioRxiv - Cell Biology 2024Quote: ... to introduce negative selection cassettes flanking the AAVS1 homology arms from the Gateway-compatible pAAVS1-P-CAG-DEST vector from Knut Woltjen (RRID:Addgene_80490). The resulting vector (pAAVS1-CAG-DEST-DTA-TK ...