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301 - 350 of 10000+ citations since 2019

• Altered firing output of VIP interneurons and early dysfunctions in CA1 hippocampal circuits in the 3xTg mouse model of Alzheimer’s disease

  Felix Michaud, et al., bioRxiv - Neuroscience 2024

  Quote: ... AAV.mDlx.GCaMP6f (Addgene, #83899) was used to target inhibitory interneurons and AAV1-CamKII-GCaMP6f-WPRE (Penn Vector Core ...
• A senescence-specific lncRNA controls metabolic rewiring of senescent cells

  Elena Grossi, et al., bioRxiv - Cell Biology 2024

  Quote: ... its shorter isoform has been amplified from IMR90 cDNA with specific primers and cloned in pcDNA3.1(+) vector (purchased from Addgene). For overexpression studies ...
• An evolutionary-conserved VPS34-PIKfyve-TRPML1-Myosin II axis regulates the speed of amoeboid cell migration

  Philippe Dehio, et al., bioRxiv - Cell Biology 2024

  Quote: ... (a kind gift from Harald Stenmark; Addgene, Cat. # 140047) electroporation was performed using a 4D-Nucleofector (Lonza ...
• Visualization of endogenous G proteins on endosomes and other organelles

  Wonjo Jang, et al., bioRxiv - Pharmacology and Toxicology 2024

  Quote: The following plasmids were used as received from Addgene: mRuby-Golgi-7 (GalT ...
• An inappropriate decline in ribosome levels drives a diverse set of neurodevelopmental disorders

  Chunyang Ni, et al., bioRxiv - Developmental Biology 2024

  Quote: ... and pCXLE-hUL (Addgene #27082 ...
• Next Generation Neuropeptide Y Receptor Small Molecule Agonists Inhibit Mosquito Biting Behavior

  Emely V. Zeledon, et al., bioRxiv - Pharmacology and Toxicology 2024

  Quote: ... and Aedes aegypti NPYLR7 (Addgene #52392) (Duvall et al. ...
• In vivo perturb-seq of cancer and microenvironment cells dissects oncologic drivers and radiotherapy responses in glioblastoma

  S. John Liu, et al., bioRxiv - Genomics 2024

  Quote: Lentivirus was produced from transfected HEK293T cells with packaging vectors (pMD2.G #12259, Addgene, and pCMV-dR8.91, Trono Lab) following the manufacturers protocol (#MIR6605 ...
• In vivo perturb-seq of cancer and microenvironment cells dissects oncologic drivers and radiotherapy responses in glioblastoma

  S. John Liu, et al., bioRxiv - Genomics 2024

  Quote: ... GL261 cultures were partially transduced with sgRNA expression lentiviruses with a GFP tag (Addgene 187241)40 ...
• Ultrasound pulse repetition frequency preferentially activates different neuron populations independent of cell type

  Jack Sherman, et al., bioRxiv - Neuroscience 2024

  Quote: ... We then infused a total of 0.5uL of AAV9-syn-GCaMP7f (titer 2.3×1013 GC/ml, Addgene 104488-AAV9, Watertown, MA) at two separate locations within the craniotomy ...
• P66Shc Mediates SUMO2-induced Endothelial Dysfunction

  Jitendra Kumar, et al., bioRxiv - Cell Biology 2024

  Quote: ... 35 and Ubc9 (Addgene plasmid # 20082) 36 was a gift from Dr ...
• A truncation mutant of adenomatous polyposis coli (APC) impairs apical cell extrusion through elevated epithelial tissue tension

  Wan J. Gan, et al., bioRxiv - Cell Biology 2024

  Quote: ... pLL5.0-eGFP-CAAX (Addgene plasmid #187252) or pLL5.0-N-APC-GFP ...
• A truncation mutant of adenomatous polyposis coli (APC) impairs apical cell extrusion through elevated epithelial tissue tension

  Wan J. Gan, et al., bioRxiv - Cell Biology 2024

  Quote: ... together with third-generation lentiviral packaging vectors (Addgene plasmids #12251, #12253, and #12259) were transfected into HEK293T cells to generate viral particles ...
• A truncation mutant of adenomatous polyposis coli (APC) impairs apical cell extrusion through elevated epithelial tissue tension

  Wan J. Gan, et al., bioRxiv - Cell Biology 2024

  Quote: ... The sequences were cloned into pSpCas9(BB)-2A-Puro (PX459) vector (Addgene plasmid #62988), followed by lipofectamine-mediated transfection into the MCF10A cells ...
• A truncation mutant of adenomatous polyposis coli (APC) impairs apical cell extrusion through elevated epithelial tissue tension

  Wan J. Gan, et al., bioRxiv - Cell Biology 2024

  Quote: ... Lentiviral construct pLL5.0 E-cadherin shRNA/mE-cadherin-mCherry (Addgene plasmid #101282), pLL5.0-eGFP-CAAX (Addgene plasmid #187252 ...
• Seeding competent TDP-43 persists in human patient and mouse muscle

  Eileen M. Lynch, et al., bioRxiv - Pathology 2024

  Quote: ... BL21(DE3)-RIL Escherichia coli cells were transformed with pJ4M-TDP43-MBP-His (Addgene 104480) and grown on LB/Kanamycin/Chloramphenicol plates then used to inoculate 2L of TB/Kan/Cam/2g dextrose ...
• Calcyphosine is a microtubule-associated protein required for spindle formation and function

  Bipul Setu, et al., bioRxiv - Cell Biology 2024

  Quote: ... CAPS-FLAG was created by Gateway cloning the CAPS gene into pDEST-N-terminal FLAG (Addgene #18700), providing expression of N-terminally FLAG-tagged CAPS control of the cmv-promoter ...
• Calcyphosine is a microtubule-associated protein required for spindle formation and function

  Bipul Setu, et al., bioRxiv - Cell Biology 2024

  Quote: ... pGEX6P1-DEST-FLAG was a gift from Andrew Jackson & Martin Reijns (Addgene plasmid # 119754 ...
• Calcyphosine is a microtubule-associated protein required for spindle formation and function

  Bipul Setu, et al., bioRxiv - Cell Biology 2024

  Quote: ... CAPS-gfp was created by Gateway cloning the CAPS gene into pDEST-CMV-C-EGFP (Addgene #122844), to create an C-terminally-tagged gfp construct under control of the cmv promoter.
• Calcyphosine is a microtubule-associated protein required for spindle formation and function

  Bipul Setu, et al., bioRxiv - Cell Biology 2024

  Quote: ... pGEX6P1-DEST-FLAG was a gift from Andrew Jackson & Martin Reijns (Addgene plasmid # 119754; http://n2t.net/addgene:119754; RRID:Addgene_119754).CAPS-GST protein was expressed in BL21Star bacteria (NEB) ...
• Construction and Application of a Technical Platform for Determining Cell Cycle- and Autophagy-Associated Cellular Uptake of Lipid-Based Nanoparticles

  Yisha Wang, et al., bioRxiv - Cell Biology 2024

  Quote: ... pLenti-PGK-Neo-PIP-FUCCI (PIP-FUCCI) (Cat. NO. 118616) and pLenti PGK Neo DEST (empty vector) (Cat. NO. 19067) were from Addgene (Cambridge, MA, USA).
• Motor execution, but not learning, improves upon increased layer 5 specific Martinotti cell excitation

  Anna Velica, et al., bioRxiv - Neuroscience 2024

  Quote: ... the mice were injected unilaterally in the right hemisphere with 600 nl of AAV9-CamKII-GCaMP6f-WPRE-SV40 (Addgene viral prep # 100834-AAV9), at a speed of 200 nl/min ...
• Motor execution, but not learning, improves upon increased layer 5 specific Martinotti cell excitation

  Anna Velica, et al., bioRxiv - Neuroscience 2024

  Quote: ... 3 Chrna2-Crewt/wt) were first injected bilaterally with 300 nl of AAV9-hSyn-DIO-hM3Dq-mCherry (Addgene, LOT 44361-AAV9 ...
• Disinhibition enables vocal repertoire expansion after a critical period

  Fabian Heim, et al., bioRxiv - Neuroscience 2024

  Quote: ... pAAV-mDlx-ChR2-mCherry-Fishell-3 was a gift from Gordon Fishell (Addgene plasmid # 83898 ...
• Motor execution, but not learning, improves upon increased layer 5 specific Martinotti cell excitation

  Anna Velica, et al., bioRxiv - Neuroscience 2024

  Quote: ... 7 Chrna2-Crewt/wt) were injected bilaterally with 300 nl of AAV9-hSyn-DIO-hM3Dq-mCherry (Addgene, LOT 44361-AAV9 ...
• Brain-wide neuronal circuit connectome of human glioblastoma

  Yusha Sun, et al., bioRxiv - Cancer Biology 2024

  Quote: ... Lentiviruses were produced using a similar procedure with 293T cells using the psPAX2 plasmid (Addgene, 12260) instead of pUMVC.
• Brain-wide neuronal circuit connectome of human glioblastoma

  Yusha Sun, et al., bioRxiv - Cancer Biology 2024

  Quote: ... 6 μg pMD2.G (Addgene, 12259), 4 μg pUMVC (Addgene ...
• Brain-wide neuronal circuit connectome of human glioblastoma

  Yusha Sun, et al., bioRxiv - Cancer Biology 2024

  Quote: ... 4 μg pUMVC (Addgene, 8449) and 18 μg transfer plasmid were mixed in 700 μL DMEM medium (Corning ...
• Brain-wide neuronal circuit connectome of human glioblastoma

  Yusha Sun, et al., bioRxiv - Cancer Biology 2024

  Quote: ... The backbone vector for shRNAs was purchased from Addgene (pLKO.1_mCherry, 128073). We followed the shRNA construction protocol from the Genetic Perturbation Platform web portal (https://portals.broadinstitute.org/gpp/public/resources/protocols) ...
• Brain-wide neuronal circuit connectome of human glioblastoma

  Yusha Sun, et al., bioRxiv - Cancer Biology 2024

  Quote: ... AAV9-EF1α-DIO-hChR2(H134R)-EYFP-WPRE-hGHpA was a gift from Karl Deisseroth (Addgene, viral prep 20298-AAV9). The retroviral CAG-dsRed-T2A-RabiesG-IRES-TVA (RTG helper plasmid ...
• A disease-associated PPP2R3C-MAP3K1 phospho-regulatory module controls centrosome function

  Anil Kumar Ganga, et al., bioRxiv - Cell Biology 2024

  Quote: ... pCW-Tet-Cas9-BFP was prepared by Gibson assembly from pCW-Cas9 (Addgene #50661).
• A disease-associated PPP2R3C-MAP3K1 phospho-regulatory module controls centrosome function

  Anil Kumar Ganga, et al., bioRxiv - Cell Biology 2024

  Quote: ... DNA fragments containing MAP3K1 (MEKK1) or kinase-dead MAP3K1 were excised from pCDNA-MEKK1 plasmids (Addgene #12181 and #12180 ...
• Claudin-4 modulates autophagy via SLC1A5/LAT1 as a tolerance mechanism for genomic instability in ovarian cancer

  Fabian R. Villagomez, et al., bioRxiv - Cancer Biology 2024

  Quote: ... containing the viral packaging system of second generation as well as the lentiviral construct of interest pCDH-EF1a-mCherry-EGFP-LC3B (RRID:Addgene_170446), respectively ...
• A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions

  Ryan J. Farrell, et al., bioRxiv - Cell Biology 2024

  Quote: To construct the ER-GCaMP fused to mRuby3, mRuby3 was PCR amplified from the pKanCMV-mRuby3-18aa-actin (Bajar et al., 2016) (Addgene #74255). CMV ER-GCaMP6-210 was restriction digested with AgeI-HF and combined with the PCR amplified mRuby3 via ligation ...
• Detection of Fluorescent Protein Mechanical Switching in Cellulo

  T. Curtis Shoyer, et al., bioRxiv - Cell Biology 2024

  Quote: ... The original TSMod was derived from pcDNA3.1-TSMod (Addgene Plasmid #26021)30 ...
• Detection of Fluorescent Protein Mechanical Switching in Cellulo

  T. Curtis Shoyer, et al., bioRxiv - Cell Biology 2024

  Quote: ... F-tractin and Linker 1 were derived from pEGFP-C1 F-tractin-EGFP (Addgene Plasmid #5847)48 ...
• D-type cyclins regulate DNA mismatch repair in the G1 and S phases of the cell cycle, maintaining genome stability

  Gergely Rona, et al., bioRxiv - Cell Biology 2024

  Quote: ... mCherry-FokI and the catalytically dead mCherry-FokI (D540A) cDNAs were subcloned into the mCherry-LacI vector (Addgene plasmid #18985) replacing mCherry ...
• Catabolic pathway acquisition by soil pseudomonads readily enables growth with salicyl alcohol but does not affect colonization of Populus roots

  Stephan Christel, et al., bioRxiv - Microbiology 2024

  Quote: ... The mini-Tn7 vector (pUC18T-mini-Tn7T-GM, Addgene plasmid # 63121), Tn7 transposase expression plasmid (pTNS ...
• Monocyte to macrophage differentiation and changes in cellular redox homeostasis promote cell type-specific HIV latency reactivation

  Alexandra Blanco, et al., bioRxiv - Microbiology 2024

  Quote: ... were produced in HEK293T cells along with the VSV-G (Addgene plasmid # 8454) and ΔR8.2 (Addgene plasmid # 8455 ...
• Monocyte to macrophage differentiation and changes in cellular redox homeostasis promote cell type-specific HIV latency reactivation

  Alexandra Blanco, et al., bioRxiv - Microbiology 2024

  Quote: The full-length HIV vectors NL4-3 ΔEnv EGFP (HIV Reagent Program) and HIVGKO (Addgene plasmid #112234) were produced in HEK293T cells along with the VSV-G (Addgene plasmid # 8454 ...
• Utilizing a dual endogenous reporter system to identify functional regulators of aberrant stem cell and differentiation activity in colorectal cancer

  Sandor Spisak, et al., bioRxiv - Cancer Biology 2024

  Quote: ... 466 sgRNAs were selected from the H3 knock out library (Addgene # 133914) from Brown/Liu labs.
• Utilizing a dual endogenous reporter system to identify functional regulators of aberrant stem cell and differentiation activity in colorectal cancer

  Sandor Spisak, et al., bioRxiv - Cancer Biology 2024

  Quote: The pCC_01 – hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE plasmid (Addgene #139086) was used for CRISPR screen and validation ...
• Utilizing a dual endogenous reporter system to identify functional regulators of aberrant stem cell and differentiation activity in colorectal cancer

  Sandor Spisak, et al., bioRxiv - Cancer Biology 2024

  Quote: pCC_01 and pCC_09 (Addgene #139094) were modified to incorporate Capture Sequences 1 and 2 at the 3’ end of guide sequences ...
• Human coronavirus 229E infection inactivates pyroptosis executioner gasdermin D but ultimately leads to lytic cell death partly mediated by gasdermin E

  Xavier Martiáñez-Vendrell, et al., bioRxiv - Microbiology 2024

  Quote: ... envelope protein-expressing vector pCMV-VSVG (38) and the transfer vectors pEF1a-CAS9-2A-Blasticidin (#52962, Addgene) or pU6-gRNA-PGK-Puro-2A-BFP encoding CRISPR-Cas9 guide RNAs (gRNAs ...
• DePARylation is critical for S phase progression and cell survival

  Litong Nie, et al., bioRxiv - Cancer Biology 2024

  Quote: ... WT and mutant constructs were subsequently cloned into the vector pLenti CMV Neo DEST (705-1) (Addgene, #17392) by the GATEWAY cloning system ...
• DePARylation is critical for S phase progression and cell survival

  Litong Nie, et al., bioRxiv - Cancer Biology 2024

  Quote: ... The DNA Damage Response MKOv4 Library (Addgene, #140219) was from our previous study35.
• Monocyte to macrophage differentiation and changes in cellular redox homeostasis promote cell type-specific HIV latency reactivation

  Alexandra Blanco, et al., bioRxiv - Microbiology 2024

  Quote: ... and ΔR8.2 (Addgene plasmid # 8455) plasmids for lentivirus generation ...
• Translational response to mitochondrial stresses is orchestrated by tRNA modifications

  Sherif Rashad, et al., bioRxiv - Molecular Biology 2024

  Quote: ... They were incorporated into lentiCRISPR-v2 (Addgene, Cat# 52961) following the GeCKO protocol with minor adjustments(Sanjana et al. ...
• Translational response to mitochondrial stresses is orchestrated by tRNA modifications

  Sherif Rashad, et al., bioRxiv - Molecular Biology 2024

  Quote: ... and packaging plasmid (psPAX2) (Addgene, 12260) using Lipofectamine 3000 per the manufacturer’s protocol ...
• A multiprotein signaling complex sustains AKT and mTOR/S6K activity necessary for the survival of cancer cells undergoing stress

  Oriana Y. Teran Pumar, et al., bioRxiv - Cancer Biology 2024

  Quote: ... and mTOR DNA constructs were obtained from Addgene (plasmid #12133, #14129, #1861)24 ...
• The host GTPase Dynamin 2 modulates apical junction structure to control cell-to-cell spread of Listeria monocytogenes

  Serena Tijoriwalla, et al., bioRxiv - Microbiology 2024

  Quote: ... pcDNA3.1-Ha-Dynamin 2.K44A (gift of S. Schmid, Addgene 34685), pEGFPC1 (Clontech) ...