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Citations for Addgene :
251 - 300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... tropicalis proteins were cloned into the pHAT2 vector (Addgene #112583) using NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621) ...
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bioRxiv - Cell Biology 2024Quote: ... control or mir-218-1-overexpressing HTR8 cells were seeded into 12-well plates and were co-transfected with 1 µg/mL of 5X NFκB luciferase reporter (64) (Addgene plasmid #12453) construct and 20 ng/mL of Renilla luciferase vector (Promega ...
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bioRxiv - Cell Biology 2024Quote: ... as well as the destination vector pCW57.1 (Addgene plasmid # 41393 ...
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bioRxiv - Cell Biology 2024Quote: ... with the only exception of plasmid pMD2.G (Addgene plasmid # 12259 ...
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bioRxiv - Cell Biology 2024Quote: ... 13 µg pCMVR8.74 (gift from Didier Trono, Addgene plasmid #22036; http://n2t.net/addgene:22036; RRID:Addgene_22036) and 7 µg pMD2.G (gift from Didier Trono ...
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bioRxiv - Cell Biology 2024Quote: ... 13 µg pCMVR8.74 (gift from Didier Trono, Addgene plasmid #22036 ...
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bioRxiv - Cell Biology 2024Quote: ... pAS1NB (a gift from Mark Prescott35, Addgene plasmid # 71245), and pFa6a-3xHA-mCherry-natMX6 ...
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bioRxiv - Cell Biology 2024Quote: ... and mCherry ORFs were amplified by PCR using pFa6a-mEGFP-kanMX6 (a gift from Julien Berro72, Addgene plasmid # 87023), pAS1NB (a gift from Mark Prescott35 ...
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bioRxiv - Cell Biology 2024Quote: ... an ORF containing the mCherry coding sequence was generated by PCR using pSJ1321 (a gift from Sue Jaspersen73, Addgene plasmid #86413) as a template and primers with BamHI and PacI restriction enzyme recognition sites ...
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bioRxiv - Cell Biology 2024Quote: ... berghei Nup138 the smHA coding sequence was amplified from plasmid pCAG_smFP_HA(Addgene #59759) using primers P4121/4122 and inserted between BamHI/BsiWI immediately downstream of the Pbnup138 homology region in the plasmid pLIS0654 (Ambekar Sushma ...
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bioRxiv - Bioengineering 2024Quote: ... The full-length SpRY CBE4max construct was purchased from Addgene (Plasmid #139999). The full-length SpRY ABE8e plasmid was generated through Gibson Assembly of gBlock Gene Fragments (Integrated DNA Technologies ...
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bioRxiv - Bioengineering 2024Quote: The plasmid encoding the U6-sgRNA expression cassette was obtained from Addgene (#47108). The full-length SpRY CBE4max construct was purchased from Addgene (Plasmid #139999) ...
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bioRxiv - Biochemistry 2024Quote: ... and packaging plasmids (psPAX2, Addgene #12260; pMD2.G, Addgene #12259) was prepared in serum-free media (Opti-MEM™ ...
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bioRxiv - Biochemistry 2024Quote: ... and packaging plasmids (psPAX2, Addgene #12260; pMD2.G, Addgene #12259) was prepared in serum-free media (Opti-MEM™ ...
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bioRxiv - Biochemistry 2024Quote: ... a ∼1:1:1 molar ratio mixture of library transfer (lentiCRISPRv2; Addgene plasmid #52961) and packaging plasmids (psPAX2 ...
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Ribonuclease Inhibitor and Angiogenin collaboratively regulate cell-type-specific global translationbioRxiv - Biochemistry 2024Quote: RNH1-KO K562 cells were infected with lentiviruses expressing ANG (pLenti CMV Blast GFP-ANG) or the empty vector pLenti CMV Blast DEST (706-1, Addgene) as previously described 52 ...
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bioRxiv - Biochemistry 2024Quote: ... 1.7 μg psPAX2 (Addgene #12260), 48.8 μg PEI (1 μg/μL ...
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bioRxiv - Biochemistry 2024Quote: ... 0.85 μg pMD2.G (Addgene #12259), 1.7 μg psPAX2 (Addgene #12260) ...
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bioRxiv - Biochemistry 2024Quote: ... 2xStrep-Flag-FOXA1-HA was introduced to pINDUCER21 (Addgene #46948) to generate pINDUCER21_FOXA1 by Gateway cloning protocol (Thermo).
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bioRxiv - Biochemistry 2024Quote: ... human PLD3 sgRNA (5′-3′) was cloned into pSpCa9(BB)-2A-Puro (PX459) V2.0 (Addgene #62988) as described (Ran et al. ...
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bioRxiv - Biochemistry 2024Quote: ... The pET28a-TRBP was purchased from Addgene (Addgene plasmid # 50351). The pET28a-TRBP plasmid was transformed into E ...
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bioRxiv - Biochemistry 2024Quote: ... The pET28a-TRBP was purchased from Addgene (Addgene plasmid # 50351). The pET28a-TRBP plasmid was transformed into E ...
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bioRxiv - Biochemistry 2024Quote: ... packaging plasmid - pCMV-dR8.2 dvpr (Addgene #8455) in a 6:4:1 ratio using PEI-max (Polysciences #24765-100) ...
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Ribonuclease Inhibitor and Angiogenin collaboratively regulate cell-type-specific global translationbioRxiv - Biochemistry 2024Quote: ... the human Angiogenin (ANG) full coding sequence (ORIGEN RC 208874) was cloned into the entry vector pENTR4-GFP-C1 (W392-1, Addgene) and then recombined into the destination vector pLenti CMV Blast DEST (706-1 ...
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Ribonuclease Inhibitor and Angiogenin collaboratively regulate cell-type-specific global translationbioRxiv - Biochemistry 2024Quote: ... and then recombined into the destination vector pLenti CMV Blast DEST (706-1, Addgene) plasmid using the Gateway cloning method.
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bioRxiv - Bioengineering 2024Quote: YAP/TAZ-TRE-mStrawberry reporter was a gift from Ravid Straussman (Addgene plasmid # 158682). From this plasmid ...
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bioRxiv - Bioengineering 2024Quote: ... David Liu at Harvard University (Addgene plasmids #75145 and #75146). Sortase enzymes were expressed and purified as previously described (Supplementary Method S3 ...
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bioRxiv - Bioengineering 2024Quote: ... and pRSV-REV (Addgene #12253), and the YAP/TAZ-TRE-tdTomato plasmid using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Bioengineering 2024Quote: ... packaging plasmids pMDLG/pRRE (Addgene #12251) and pRSV-REV (Addgene #12253) ...
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bioRxiv - Bioengineering 2024Quote: ... and then HEKs were transfected with envelope plasmid pMD2.G (Addgene #12259), packaging plasmids pMDLG/pRRE (Addgene #12251 ...
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bioRxiv - Biochemistry 2024Quote: ... GFP-Sec61B (Addgene: 121159) were ordered from Addgene ...
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bioRxiv - Biochemistry 2024Quote: ... Sar1(T39N)-YFP (Addgene: 128155), EGFP-Sec23A (Addgene ...
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bioRxiv - Biochemistry 2024Quote: ... were ordered from Addgene. Grasp55-GFP/G2A and Grasp55-GFPΔPDZ1 +2 were generated using site directed mutagenesis ...
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bioRxiv - Biochemistry 2024Quote: ... The Venus-PDZ1 (ZO-1) was generated by site directed mutagenesis of the Venus-ZO-1 expression vector (Addgene: 56394) using primer pairs that delete the entire ZO-1 coding sequence except for the first PDZ1 domain ...
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bioRxiv - Biochemistry 2024Quote: ER-mScarletl70 (Addgene 137805) was used as an ER organellar marker.
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bioRxiv - Biochemistry 2024Quote: ... envelope protein-pCMV-VSV-G (Addgene #8454), packaging plasmid - pCMV-dR8.2 dvpr (Addgene #8455 ...
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bioRxiv - Biochemistry 2024Quote: HEK293T cells were transfected with pLJC5-hPLD3 or pLJC5-TMEM192-3xHA (Addgene #102930) envelope protein-pCMV-VSV-G (Addgene #8454) ...
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bioRxiv - Biochemistry 2024Quote: ... EGFP-Sec23A (Addgene: 666009), GFP-Sec61B (Addgene ...
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bioRxiv - Biochemistry 2024Quote: ... Grasp55-GFP (Addgene: 137708), Sar1(T39N)-YFP (Addgene ...
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bioRxiv - Biochemistry 2024Quote: ... SORT1 cDNA was obtained from the hORFeome V8.1 collection and cloned into pLX301 (Addgene #25895) for co-transfection with psPAX2 and pVSV-G (Addgene #138479) ...
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bioRxiv - Biochemistry 2024Quote: ... for co-transfection with psPAX2 and pVSV-G (Addgene #138479). Virus was collected from HEK293Ts 72hr post-transfection for all gene-specific constructs.
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bioRxiv - Biochemistry 2024Quote: ... and SecD (tagtGGAGTCGGTGATATGGTCAC) were integrated into the pFD152 vector67 (Addgene #125546) using Gibson assembly ...
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bioRxiv - Biochemistry 2024Quote: ... NM_018344.6 (SLC29A3):c.1427A>G (p.Ter476Trp) and NM_000343.4(SLC5A1):c.1993T>C (p.Ter665Arg)) were cloned into pCDNA3.1-HA (Addgene 128034) using Gibson assembly ...
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bioRxiv - Biochemistry 2024Quote: ... AriB or AriA-AriB were cloned into an arabi-nose-inducible UC Macrolab vector 8-B (Addgene #37502; ampicillin-resistant) and Ocr was cloned into an IPTG-inducible pTrc99A-based vector (kanamycin-resistant) ...
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bioRxiv - Biochemistry 2024Quote: ... and T7 Ocr (NCBI Accession # NP_041954.1) genes were cloned into UC Berkeley Macrolab vectors 2-BT (Addgene #29666) and 13S-A (Addgene #48323 ...
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bioRxiv - Biochemistry 2024Quote: ... The pSPAX2 and pMD2.G plasmids for lentiviral packaging were acquired from Addgene (#12260 and #12259).
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bioRxiv - Biochemistry 2024Quote: Plasmids encoding GPCRs were obtained from various sources (as indicated Key Resources Table) or subcloned into pcDNA3.1 from the PRESTO-Tango GPCR kit (Addgene Kit #1000000068) as described next ...
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bioRxiv - Biochemistry 2024Quote: ... and 13S-A (Addgene #48323) for N-terminal TEV protease-cleavable His6-tag and tagless constructs ...
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bioRxiv - Biochemistry 2024Quote: ... The plasmid pT3TS-nCas9n (Addgene #46757) was used as a template to synthesize Cas9 mRNA in vitro using the mMESSAGE mMACHINE™ T3 Transcription Kit (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: ... pFA6a-link-yoEGFP-SpHis5 (primers OM-8 and OM-9) (Addgene plasmid #44836), and pBTK522::FAST-PETase 21 (gift from Hal Alper ...