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Citations for Addgene :
451 - 500 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: UAS-Gαiact was generated from the Gαi cDNA clone (Addgene) LD22201 ...
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bioRxiv - Plant Biology 2024Quote: ... and terminators) from the GB2.0 kit purchased from Addgene (https://www.addgene.org/kits/orzaez-goldenbraid2/) ...
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bioRxiv - Neuroscience 2024Quote: ... Wickersham (Addgene plasmid no ...
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bioRxiv - Neuroscience 2024Quote: ... or pAAV-hSyn-EGFP a gift from Bryan Roth (titer ≥ 7×10¹² genome copies per ml; Addgene viral prep # 50465-AAV1 ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV-CAG-dLight1.1 a gift from Lin Tian (titer 1.3 × 1012 genome copies per ml; Addgene viral prep # 111067-AAV1 ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV-CAG-dLight1.1 a gift from Lin Tian (titer 1.3 × 1012 genome copies per ml; Addgene viral prep # 111067-AAV1; http://n2t.net/addgene:111067; RRID:Addgene_111067) or pAAV-hSyn-EGFP a gift from Bryan Roth (titer ≥ 7×10¹² genome copies per ml ...
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bioRxiv - Neuroscience 2024Quote: ... or pAAV-hSyn-EGFP a gift from Bryan Roth (titer ≥ 7×10¹² genome copies per ml; Addgene viral prep # 50465-AAV1; http://n2t.net/addgene:50465; RRID:Addgene_50465) were injected into the dorsolateral (100-200 nl ...
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bioRxiv - Neuroscience 2024Quote: ... and then 500 nL of AAV1-hSyn-GCaMP6s-WPRE-SV40 (Addgene: 100843-AAV1) was injected at a rate of 5 nL/second ...
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bioRxiv - Neuroscience 2024Quote: ... the captured genomic regions were cloned into the hSTARR-seq_ORI vector (Addgene #99296; (79)) following linearization of the vector through restriction enzyme digestion using AgeI-HF (NEB ...
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bioRxiv - Neuroscience 2024Quote: ... a co-injection of AAV5- hsyn-FLEX-PSAM-GlyR-IRES-EGFP (AAV-FLEX-PSAM-GlyR-EGFP; Addgene, #119741) and AAV1-TRE-Cre (SignaGen Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... Rats then received bilateral infusions of either AAV8-CAMKIIa-hM3D(Gq)-mCherry or AAV8-CaMKIIa-GFP (5×1012 vg/mL for both viruses; Addgene) into the VH (either A/P ...
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bioRxiv - Neuroscience 2024Quote: ... AAV expressing Cre-dependent GCaMP6s (100842-AAV9, AAV9.CAG.Flex.GCaMP6s, Addgene) was injected unilaterally above the arcuate nucleus (ARC ...
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bioRxiv - Neuroscience 2024Quote: ... The following three viruses were utilized: pAAV.1-CAG-GFP (#37825, Addgene), OE-Npbwr1-GFP ...
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bioRxiv - Neuroscience 2024Quote: ... 500 nl of AAV5-hSyn-DIO-hM4D(Gi)-mCherry virus (Addgene #44362-AAV5) was infused bilaterally at 100 nl/min ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV5-hSyn-DIO-hM4D(Gi)-mCherry (Addgene, 44362, vg/mL) or pAAV5-hSyn-DIO-hM3D(Gq)-mCherry (Addgene ...
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bioRxiv - Neuroscience 2024Quote: ... Wild-type animals were then injected whether with mix 1 or mix 2 or AAV9-CamKII-GCaMP6f-WPRE-SV40 (Addgene, 100834, vg/mL) or AAV9-CamKII-hM4D(Gi)-mCherry (construct provided by Bryan Roth ...
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bioRxiv - Neuroscience 2024Quote: ... or pAAV5-hSyn-DIO-hM3D(Gq)-mCherry (Addgene, 44361, vg/mL) were injected in Nav1.8 Cre animals in in the NTS (from bregma ...
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bioRxiv - Neuroscience 2024Quote: ... 500 nl of AAV5-EF1a-DIO-ChR2(H134R)-mCherry virus (Addgene #44361-AAV5) was injected unilaterally at 100nl/min ...
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bioRxiv - Neuroscience 2024Quote: ... plus either AAV8-hSyn-DIO-hM4Di-mCherry (2.2 x 1013 GC/mL, Addgene, Watertown, MA) or AAV8-hSyn-DIO-mCherry (Control virus ...
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bioRxiv - Neuroscience 2024Quote: ... The APP gRNA sequence was cloned into pSpCas9 (BB)-2A-Puro (PX495) v.2.0 (plasmid #62988; Addgene, Cambridge, MA, USA) using the method described by Ran et al.48 ...
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bioRxiv - Neuroscience 2024Quote: ... and pCAX APP Swe/Ind (#30145, Addgene), respectively ...
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bioRxiv - Microbiology 2024Quote: ... cells was transfected with 15ng STING (pMSCV-hygro-STING R232, Addgene 102608), 20ng encoding firefly luciferase driven by an IFN-beta promoter (IFN-Beta_pGL3 ...
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bioRxiv - Molecular Biology 2024Quote: ... pET30a-AlkB and pET30a-AlkB-D135S were a gift from Tao Pan (Addgene plasmid # 79051; http://n2t.net/addgene:79051; RRID:Addgene_79051, and Addgene plasmid # 79050; http://n2t.net/addgene:79050 ; RRID:Addgene_79050). pET30a-AlkB-D135S/L118V (20 ...
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bioRxiv - Molecular Biology 2024Quote: ... pET30a-AlkB and pET30a-AlkB-D135S were a gift from Tao Pan (Addgene plasmid # 79051; http://n2t.net/addgene:79051; RRID:Addgene_79051, and Addgene plasmid # 79050 ...
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bioRxiv - Microbiology 2024Quote: ... obtained from the pAG25 plasmid (Addgene plasmid #35121), flanked with sequences of the target allele ...
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bioRxiv - Microbiology 2024Quote: ... GOI_gRNA2_F and GOI_gRNA2_R) containing gRNA sequence were annealed and cloned into BsaI site of the pU6-Universal vector (Addgene #52694). To generate a construct for deleting the entire coding region of GOI ...
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bioRxiv - Molecular Biology 2024Quote: ... pLX209-neo-WRN K577M were a gift from Francisca Vazquez (respectively, Addgene plasmids # 125788, http://n2t.net/addgene:125788; RRID:Addgene_125788 ...
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bioRxiv - Molecular Biology 2024Quote: ... pLX209-neo-WRN K577M were a gift from Francisca Vazquez (respectively, Addgene plasmids # 125788 ...
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bioRxiv - Cell Biology 2024Quote: Plk1 FRET sensor c-jun substrate was a gift from Michael Lampson (Addgene plasmid #45203 ...
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bioRxiv - Cell Biology 2024Quote: Plk1 FRET sensor c-jun substrate was a gift from Michael Lampson (Addgene plasmid #45203; http://n2t.net/addgene:45203; RRID:Addgene 45203). Cells were transiently transfected with a and plated onto glass-bottom plates ...
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bioRxiv - Molecular Biology 2024Quote: ... SK-BR-3 HER2-knockout (SK-BR-3 KO) were obtained using pSpCas9 BB-2A-Puro (PX459) V2.0 (9200 bp, Addgene) containing a sgRNA sequence (5’-TCATCGCTCACAACCAAGTG-3’ ...
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bioRxiv - Molecular Biology 2024Quote: Mouse Two Plasmid Activity-Optimized CRISPR Knockout Library (Addgene#1000000096) was amplified in E coli ...
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bioRxiv - Neuroscience 2024Quote: ... and hOPTNΔC (Addgene, #23053) were cloned into meGFP-hTRAK1 to generate meGFP-hOPTN and meGFP- hOPTNΔC ...
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bioRxiv - Neuroscience 2024Quote: ... TRAK1 (Addgene, #127621), his-hOPTN (Addgene ...
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bioRxiv - Neuroscience 2024Quote: ... 4xmts-mScarlet-I (Addgene, #98818), TurboID (Addgene ...
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bioRxiv - Neuroscience 2024Quote: ... TurboID (Addgene, #107169) and Cre 33 were cloned and packaged into our pAM-AAV- mSncg-WPRE backbone containing the RGC-specific full-length mSncg promoter 33 ...
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bioRxiv - Microbiology 2024Quote: ... a 350-450 bp fragment corresponding to the U16 sequence was cloned into the double T7 vector L4440 (a gift from Andrew Fire, Addgene plasmid # 1654). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... We acquired wild-type α-syn/pET21a (Addgene 51486) courtesy of the Michael J ...
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bioRxiv - Microbiology 2024Quote: ... The Ebola GP-encoding plasmid was obtained from Addgene. Information about RBPs is provided in Table S1.
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bioRxiv - Microbiology 2024Quote: ... Guide RNAs targeting exon 6 of Akr1c13 were cloned into pX330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene #42230). A T7-sgRNA PCR product was amplified and in vitro transcribed as previously described (65 ...
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bioRxiv - Molecular Biology 2024Quote: Lentiviruses were produced in HEK293FT cells cultured in T225 flasks by cotransfection of 30μg of packaging plasmid (psPAX2, Addgene #12260), 30 μg of envelope plasmid (VSV-G ...
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bioRxiv - Microbiology 2024Quote: ... a gift from Didier Trono (Addgene plasmid #12260 ...
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bioRxiv - Molecular Biology 2024Quote: ... pCS2-nCas9n was a gift from Wenbiao Chen (Addgene plasmid #47929; http://n2t.net/addgene:47929; RRID: Addgene_47929). pCS2-nCas9n-nanos 3’UTR was a gift from Antonio Giraldez (Addgene plasmid # 62542 ...
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bioRxiv - Molecular Biology 2024Quote: The plasmid pLVX-EF1alpha-SARS-CoV-2-orf6-2xStrep-IRES-Puro (Addgene plasmid #141387 from Nevan Krogan) [12] was used for the overexpression of the accessory proteins ORF6 ...
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bioRxiv - Molecular Biology 2024Quote: ... pCS2-nCas9n-nanos 3’UTR was a gift from Antonio Giraldez (Addgene plasmid # 62542 ...
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bioRxiv - Cell Biology 2024Quote: ... GFP-BLM is from Addgene plasmid #80070 ...
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bioRxiv - Molecular Biology 2024Quote: ... pCS2-nCas9n was a gift from Wenbiao Chen (Addgene plasmid #47929 ...
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bioRxiv - Molecular Biology 2024Quote: ... pCS2-nCas9n-nanos 3’UTR was a gift from Antonio Giraldez (Addgene plasmid # 62542; http://n2t.net/addgene:62542; RRID: Addgene_62542). 250 ng/μl of Cas9 mRNA and 50 ng/μl of gRNA(s ...
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bioRxiv - Molecular Biology 2024Quote: ... and full-length mTagBFP2 in Puc19 (Addgene Plasmid #50005), driven by a SFFV promoter (from plasmid #pFu-62 derived from Addgene Plasmid #57827 ...
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bioRxiv - Molecular Biology 2024Quote: ... and a WPRE sequence in pLentiGuide-Puro (Addgene Plasmid #52963), driven by the EF1a promoter ...