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Citations for Addgene :
501 - 550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: gRNA plasmids were constructed by cloning the targeted sequences into the pX330 vector (Addgene #42230) via the BbsI sites under the human U6 promoter ...
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bioRxiv - Bioengineering 2024Quote: ... a gift from David Liu (Addgene plasmid # 132775; http://n2t.net/addgene:132775; RRID; Addgene_132775), was digested by EcoRI and PmeI (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2024Quote: ... pSpCas9(BB)-2A-GFP (PX458) was a gift from Feng Zhang (Addgene plasmid # 48138 ...
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bioRxiv - Bioengineering 2024Quote: ... pSpCas9(BB)-2A-GFP (PX458) was a gift from Feng Zhang (Addgene plasmid # 48138; http://n2t.net/addgene:48138; RRID:Addgene_48138). PX458 was digested by EcoRI ...
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bioRxiv - Bioengineering 2024Quote: All plasmid vector backbones will be available from Addgene upon publication ...
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bioRxiv - Bioengineering 2024Quote: ... which was a gift from Magnus Essand (Addgene plasmid #80391 ...
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bioRxiv - Bioengineering 2024Quote: ... which was a gift from James Thomson (Addgene plasmid #20922; http://n2t.net/addgene:20922; RRID:Addgene_80391). β-globin IR was amplified from BAC clone RP11-1205H24 obtained from BACPAC Genomics Inc ...
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bioRxiv - Bioengineering 2024Quote: ... which was a gift from Magnus Essand (Addgene plasmid #80391; http://n2t.net/addgene:80391; RRID:Addgene_80391). The split puromycin intein sequences were synthesized by Twist Bioscience based on the sequences in Addgene plasmids 134319 and 13431939.
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bioRxiv - Bioengineering 2024Quote: pU6-pegRNA-GG-acceptor was a gift from David Liu (Addgene plasmid # 132777; http://n2t.net/addgene:132777; RRID; Addgene_132777). pegRNA-GFP and pegRNA-APOE were designed using the PrimeDesign web platform version (https://drugthatgene.pinellolab.partners.org/)20 ...
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bioRxiv - Bioengineering 2024Quote: ... and exon 2-8 was cloned from Addgene #182141 ...
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bioRxiv - Bioengineering 2024Quote: pU6-pegRNA-GG-acceptor was a gift from David Liu (Addgene plasmid # 132777 ...
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bioRxiv - Bioengineering 2024Quote: ... a gift from David Liu (Addgene plasmid # 132775 ...
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bioRxiv - Biochemistry 2024Quote: ... and 0.34 µg psPAX2 (Addgene #12260) using PEI following standard protocol ...
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bioRxiv - Biochemistry 2024Quote: ... and Peredox-mCherry (#32380) 35 constructs were obtained from AddGene. Enolase-RFP ...
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bioRxiv - Biochemistry 2024Quote: ... 0.17 µg VSV.G (Addgene #14888) and 0.34 µg psPAX2 (Addgene #12260 ...
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bioRxiv - Biochemistry 2024Quote: ... which was a kind gift from Joseph Gordon (Addgene #100796) and V5-miniTurbo-NES in pcDNA3.1 ...
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bioRxiv - Biochemistry 2024Quote: ... pGEX4T3-GST-Tev was a gift from Robert Sobol (Addgene plasmid # 177142 ...
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bioRxiv - Biochemistry 2024Quote: ... pGEX4T3-GST-Tev was a gift from Robert Sobol (Addgene plasmid # 177142; http://n2t.net/addgene:177142; RRID:Addgene_177142) (Fang et al. ...
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bioRxiv - Biochemistry 2024Quote: ... were inserted into the pHLmMBP-8 vector (Addgene, cat # 72347)85 using restriction enzymes ...
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bioRxiv - Biochemistry 2024Quote: ... coli expression vector pAIDA1 was a gift from Gen Larsson (Addgene plasmid # 79180 ...
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bioRxiv - Biochemistry 2024Quote: ... coli expression vector pAIDA1 was a gift from Gen Larsson (Addgene plasmid # 79180; http://n2t.net/addgene:79180; RRID:Addgene_79180) (Jarmander et al. ...
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bioRxiv - Biophysics 2024Quote: ... Peter Hegemann (Addgene plasmid #115337).
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bioRxiv - Biochemistry 2024Quote: ... Cells were subcultured twice a week at a ratio of 1:5 to 1:8.Transient transfection of HeLa cells (1–2 x 105 cells/6-well) with pcDNA3.1(+) NAPstar or pSC2 HyPer7 (Addgene; plasmid #136466 ...
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bioRxiv - Biochemistry 2024Quote: ... pLJM1 was a gift from David Sabatini (Addgene plasmid # 19319).(69 ...
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bioRxiv - Neuroscience 2024Quote: ... followed by injection of pAAV-hsyn-DIO-hm4D(Gi)26 (Addgene viral prep # 44362-AAV5) into the bilateral dmPFC (A-P 1.35 ...
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bioRxiv - Neuroscience 2024Quote: Viral pAAV-hsyn-DIO-hm4D(Gi) (Addgene_44362-AAV5) injected mice were administered intraperitoneally with Clozapine N-oxide dihydrochloride (CNO ...
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bioRxiv - Neuroscience 2024Quote: ... Opto-labeled dmPFC MP neurons were labeled with pAAV-CAG-hChR2-mcherry24 (Addgene viral prep # 28017-AAVrg) injected into motor cortex (A-P -0.6 ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV-Ef1a-mCherry-IRES-Cre25 (Addgene viral prep # 55632-AAVrg) was injected into bilateral motor cortex (A-P -0.6 ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV-CKIIa-stGtACR2-FusionRed16 (Addgene viral prep # 105669-AAVrg) was injected into the bilateral motor cortex (A-P-0.6 ...
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bioRxiv - Cancer Biology 2024Quote: The human CRISPR Brunello lentiviral pooled library (Addgene # 73178-LV)14 and human CRISPR Dolcetto (Set A) inhibition library (Addgene # 92386-LV)15 were used to identify genes responsible for enhanced survival of PANC-1 cells treated with nab-paclitaxel ...
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bioRxiv - Cancer Biology 2024Quote: The human CRISPR Brunello lentiviral pooled library (Addgene # 73178-LV)14 and human CRISPR Dolcetto (Set A ...
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bioRxiv - Cancer Biology 2024Quote: ... and pDEST-V2 (Addgene plasmid 73638) vectors were assembled from various recombinant and synthetic components as described previously23 ...
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bioRxiv - Cell Biology 2024Quote: ... and psPAX2 (Addgene, #12260) packaging plasmids using lipofectamine 2000 (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... and pLKO.1 ShCtrl and psPAX2 (#12260) and pMD2G (#12259) (Addgene, Cambridge, MA, USA) were used ...
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bioRxiv - Cell Biology 2024Quote: ... was cloned into the pEF-GFP plasmid (Addgene #11154) by replacing the GFP ...
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bioRxiv - Cell Biology 2024Quote: ... and a pair of TALENs (Addgene # 59025, # 59026) through co-electroporation into hPSCs ...
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bioRxiv - Cell Biology 2024Quote: ... hP53DD fragment was cloned into PEmax-P2A-hMLH1dn (Addgene #174828) at the C-terminus (hP53DD-PEmax-P2A-hMLH1dn ...
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bioRxiv - Cell Biology 2024Quote: ... and pU6-tmpknot-GG-acceptor (Addgene #174039). These fragments were then assembled with the linearized lentivirus vector to generate the lenti-pegRNA-GG-acceptor and lenti-tmpknot-GG-aceptor backbone plasmids under hU6 promoter ...
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bioRxiv - Cell Biology 2024Quote: ... the BSD fragment in pCMV-PEmax-P2A-BSD (Addgene # 174821) was first replaced with the hP53DD fragment to generate pCMV-PEmax-hP53DD ...
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bioRxiv - Cell Biology 2024Quote: ... the lenti sgRNA(MS2)_puro plasmid (Addgene #73797) was linearized with BamHI and NdeI ...
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bioRxiv - Cell Biology 2024Quote: ... PEmax or PE-Plus were constructed by replacing Cas9 in the Hygro-Cas9 donor plasmid (Addgene #86883) with the respective PE2 ...
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bioRxiv - Cell Biology 2024Quote: ... through BbsI site or into lenti-sgRNA blast (Addgene #104993) through BsmbI site ...
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bioRxiv - Cell Biology 2024Quote: ... pCMV-PEmax-P2A-BSD (Addgene # 174821) or the cloned PE-Plus.
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bioRxiv - Cell Biology 2024Quote: ... Fragment containing the RFP dropout cassette and the fragment containing the RFP cassette along with the mpknot motif were separately amplified from pU6-pegRNA-GG-aceptor (Addgene #132777) and pU6-tmpknot-GG-acceptor (Addgene #174039) ...
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bioRxiv - Cell Biology 2024Quote: ... Nicking sgRNA for PE3 editing was cloned into LsgRNA backbone (Addgene #47108) through BbsI site or into lenti-sgRNA blast (Addgene #104993 ...
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bioRxiv - Cell Biology 2024Quote: ... which were amplified from pCMV-PE2 (Addgene #132775), pCMV-PEmax-P2A-BSD (Addgene # 174821 ...
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bioRxiv - Cell Biology 2024Quote: ... we used the lentiCRISPRv2 vector (that includes a puromycin-selection cassette; a gift from Dr. Feng Zhang, Addgene, Ref. 52961), and its blasticidin or hygromycin derivatives (a gift from Dr ...
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bioRxiv - Cancer Biology 2024Quote: ... Lentiviral particles were generated by transfecting HEK 293T cells with 12 μg of pLJM1-mEGFP or pLJM1ALKBH5 plasmids with 6 μg of psPAX2 packaging plasmid and 2 μg of pMD2.G envelope plasmid (Addgene). The medium was replaced 16h after transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... and 2 μg pMD2.G envelope plasmid (Addgene plasmid #12259). Medium was changed 16 h after transfection and lentiviral particles were harvested 24 h later ...
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bioRxiv - Cancer Biology 2024Quote: mEGFP and mEGFP-ALKBH5 sequences were first ligated into the AgeI and PSTI restriction sites of a pLJM1 plasmid (Addgene). Lentiviral particles were generated by transfecting HEK 293T cells with 12 μg of pLJM1-mEGFP or pLJM1ALKBH5 plasmids with 6 μg of psPAX2 packaging plasmid and 2 μg of pMD2.G envelope plasmid (Addgene) ...