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Citations for Addgene :
4051 - 4100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... The Sp-Cas9 plasmid (Addgene plasmid #62731) was utilized to synthesize SpCas9 as described previously (36) ...
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bioRxiv - Cancer Biology 2024Quote: ... [14] in bacteria were ordered from AddGene as bacterial stab stocks ...
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bioRxiv - Cancer Biology 2024Quote: ... and HIF2α WT and HIF2α P405A/P531A plasmids (Addgene plasmid IDs #26055 and #19006 ...
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bioRxiv - Cancer Biology 2024Quote: ... Lenti-sgRNA-neo (Addgene #104992) and lentiGuide-Puro (Addgene #52963 ...
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bioRxiv - Cancer Biology 2024Quote: Cells were transduced with lentivirus expressing pHR-SFFV-KRAB-dCas9-P2A-mCherry (Addgene #60954) and sorted for mCherry positive cells ...
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bioRxiv - Cancer Biology 2024Quote: ... and lentiGuide-Puro (Addgene #52963) digested with BsmBI were used to express 2 sets of sgRNAs (Supplementary Table 5 ...
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bioRxiv - Cancer Biology 2024Quote: ... sgRNAs were cloned into a modified U6-based expression vector pSLQ1651-sgTelomere (F+E) (Addgene #51024) that enhanced sgRNA stability and assembly with dCas9 protein64 ...
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bioRxiv - Cancer Biology 2024Quote: ... pWPXLd was a gift from Didier Trono (Addgene plasmid #12258 ...
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bioRxiv - Cancer Biology 2024Quote: ... full length BRCA1 was PCR-amplified from pCL-MFG-BRCA1 (Addgene #12341 (74)) with a triple Ty1-tag included in the reverse primer and subsequently cloned into pENTR_1A ...
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bioRxiv - Cancer Biology 2024Quote: ... were cloned into TLCV2-LoxP backbone (TLCV2-LoxP was a gift from Adam Karpf (Addgene plasmid #127098; http://n2t.net/addgene:127098; RRID:Addgene_127098) and respective constructs transformed into competent E.coli (C3040H ...
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bioRxiv - Cancer Biology 2024Quote: ... Scramble pLKO.1 shRNA control (further termed “scramble”) was a gift from David Sabatini (Addgene plasmid #1864; http://n2t.net/addgene:1864; RRID:Addgene_1864). oeELF3 ...
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bioRxiv - Cancer Biology 2024Quote: ... Scramble pLKO.1 shRNA control (further termed “scramble”) was a gift from David Sabatini (Addgene plasmid #1864 ...
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bioRxiv - Cancer Biology 2024Quote: ... 3µg of pMD2.G (Addgene) and 6µg of vector plasmid in 1.5ml of Opti-MEM (Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: 293T cells (1.5 x 106 cells in a 10-cm dish) were transfected with the pCDH-puro-CMV-VC3AI lentiviral vector (Addgene, 78907) using Lipofectamine 2000 ...
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bioRxiv - Neuroscience 2024Quote: ... Flexed GCaMP6S (AAV1.Syn.Flex.GCaMP6s.WPRE.SV40; Addgene; RRID:Addgene_105558-AAV1) diluted 1:10 in saline ...
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bioRxiv - Neuroscience 2024Quote: ... along with CaMKII-dependent Cre-recombinase (AAV1.CamKII 0.4.Cre.SV40; Addgene; RRID:Addgene_105558-AAV1) diluted 1:100 saline was injected at the centre of the craniotomy via a sharp glass pipette ...
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bioRxiv - Neuroscience 2024Quote: ... along with CaMKII-dependent Cre-recombinase (AAV1.CamKII 0.4.Cre.SV40; Addgene; RRID:Addgene_105558-AAV1) diluted 1:100 saline was injected at the centre of the craniotomy via a sharp glass pipette ...
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bioRxiv - Neuroscience 2024Quote: ... Flexed GCaMP6S (AAV1.Syn.Flex.GCaMP6s.WPRE.SV40; Addgene; RRID:Addgene_105558-AAV1) diluted 1:10 in saline ...
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bioRxiv - Neuroscience 2024Quote: ... Ai32 mice expressing Cre-dependent ChR2-eYFP were injected with AAV1.CamKII0.4.Cre.SV40 (Addgene, #105558-AAV1, titer: 3.5×10¹² vg/mL) in three different locations along RL and two different depths (250 µm and 500 µm ...
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bioRxiv - Biochemistry 2024Quote: ... The EP300 cDNA plasmid in pcDNA3.1-p300 was a gift from Warner Greene (Addgene plasmid # 23252) 84 ...
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bioRxiv - Cancer Biology 2024Quote: ... and mCherry (Addgene 84020) sequences were inserted into the pSLCAR-CD19-BBz (Addgene 135992 ...
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bioRxiv - Cancer Biology 2024Quote: ... sequences were inserted into the pSLCAR-CD19-BBz (Addgene 135992) plasmid via molecular cloning ...
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bioRxiv - Cancer Biology 2024Quote: ... the luciferase (Addgene 46793) and mCherry (Addgene 84020 ...
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bioRxiv - Cancer Biology 2024Quote: ... and lentiCas9-Blast (Addgene, Cat. 52962, RRID: Addgene_52962) plasmids were used to generate CDK12 and CDK9 KO cell lines ...
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bioRxiv - Cancer Biology 2024Quote: HEK-293T cells were transfected with a mixture of 6μg of psPAX2 (Addgene), 3µg of pMD2.G (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: ... and psPAX2 (Addgene, 12260) were co-transfected to facilitate virus production ...
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bioRxiv - Cancer Biology 2024Quote: Lentivirus was prepared by calcium phosphate transfection of 293T cells using psPAX (Addgene # 12260) and pMD2.G (Addgene # 12259) ...
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bioRxiv - Cancer Biology 2024Quote: ... [45] and pBABE-FLAG-LKB1 vector (a gift from Lewis Cantly, plasmid #8592 on AddGene) [14] in bacteria were ordered from AddGene as bacterial stab stocks ...
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bioRxiv - Cancer Biology 2024Quote: A pBABE-puro vector (a gift from Hartmut Land & Jay Morgenstern & Bob Weinberg, plasmid #1764 on AddGene) [45] and pBABE-FLAG-LKB1 vector (a gift from Lewis Cantly ...
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bioRxiv - Cell Biology 2024Quote: ... in a pLenti-CMV-Hygro vector (Addgene, 17454).
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bioRxiv - Cell Biology 2024Quote: ... Bassik (Addgene, 89359). Guide sequences for CLCC1 ...
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bioRxiv - Biophysics 2024Quote: ... and C were cloned into the pNH-TrxT vector (pNH-TrxT was a gift from Opher Gileadi (Addgene plasmid # 26106 ...
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bioRxiv - Biophysics 2024Quote: His-tagged TEV protease (pDZ2087, a gift from David Waugh (Addgene plasmid # 92414 ; http://n2t.net/addgene:92414 ; RRID:Addgene_92414)) was expressed in BL21(DE3)CodonPlus E ...
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bioRxiv - Biophysics 2024Quote: ... and C were cloned into the pNH-TrxT vector (pNH-TrxT was a gift from Opher Gileadi (Addgene plasmid # 26106 ; http://n2t.net/addgene:26106 ; RRID:Addgene_26106, (32)) that contained an N-terminal thioredoxin ...
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bioRxiv - Cell Biology 2024Quote: ... immortalized parental fibroblasts (JR20) (37) were transfected with pSpCas9(BB)-2A-Puro (PX459) V2.0 (Plasmid 62988, Addgene) with target sequence 5’-AAACTTCATCAATAACCCGC-3’ generated using established protocols (38) ...
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bioRxiv - Cell Biology 2024Quote: ... lenti MS2-P65-HSF1_Hygro (Addgene, #61426) and dCAS9-VP64_GFP (Addgene ...
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bioRxiv - Cell Biology 2024Quote: ... OPTN217-577-GFP (Addgene, plasmid #68841 [41]), or OPTND474N-GFP (Addgene ...
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bioRxiv - Cell Biology 2024Quote: ... OPTN1-577-GFP (Addgene, plasmid 27052 [54]), OPTN1-209-GFP (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: ... and pMD2.G (Addgene: 12259), facilitated with Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... the packaging plasmid psPAX2 (Addgene: 12260), and pMD2.G (Addgene ...
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bioRxiv - Cell Biology 2024Quote: ... cells were transiently transfect with BFP-KDEL (Addgene, 49150) and imaged 48 hr later ...
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bioRxiv - Cell Biology 2024Quote: ... and safe-targets (sgSAFE #5784) (see Table 1) were selected from the Bassik Human CRISPR Knockout Library (Addgene, 101926 ...
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bioRxiv - Cancer Biology 2024Quote: ... and annealed and ligated in the pLKO.1 Hygro linearized backbone (a gift from Bob Weinberg, plasmid #24150 on Addgene). Stbl3 bacteria were heat-shocked to uptake ligated plasmid ...
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bioRxiv - Cancer Biology 2024Quote: ... Three sgRNAs targeting each candidate enhancer were cloned into LentiGuide-Puro backbone (LentiGuide-Puro, Addgene, #52963). A non-targeting sgRNA and sgRNAs that target the Nupr1 gene body were used as negative and positive controls ...
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Tumor microenvironment acidosis favors pancreatic cancer stem cell properties and in vivo metastasisbioRxiv - Cancer Biology 2024Quote: Cells were seeded in 12-well plates and transfected at 50-70% confluence with 0.5 µg/well TOPflash (Addgene, #12456) and 0.3 µg pRL-TK (Promega ...
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bioRxiv - Cancer Biology 2024Quote: ... in which the TURBO-ID region was substituted by a 3HA-tag and the murine form of Sox11 (obtained from Addgene: #120387) was cloned after the tag (between restriction sites for EcoRI and BamHI) ...
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bioRxiv - Cancer Biology 2024Quote: ... James D Sutherland (Addgene: #208041) in which the TURBO-ID region was substituted by a 3HA-tag and the murine form of Sox11 (obtained from Addgene ...
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bioRxiv - Cell Biology 2024Quote: Peredox NADH/NAD+ sensor (Cat# 163060, Addgene) was transfected into HEK293 cells for lentivirus production ...
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bioRxiv - Cell Biology 2024Quote: ... was transfected together with envelope expressing plasmid 9 - PMS2.G (#12259, Addgene), and empty backbone packaging plasmid PSPAX2 (#12260 ...
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bioRxiv - Cell Biology 2024Quote: TurboID sequence was amplified from a pcDNA3 plasmid containing 3xHA-TurboID-NLS (Addgene #107171). Flag tag sequence was added to the N-terminus using a forward primer ...