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Citations for Addgene :
3901 - 3950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... pMDLg/pRRE (Addgene #12251), RSV/REV (Addgene plasmid #12253) ...
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bioRxiv - Cancer Biology 2024Quote: ... RSV/REV (Addgene plasmid #12253), pMD2.G (Addgene #12259) ...
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bioRxiv - Cancer Biology 2024Quote: pLKO.1 puro (Addgene #8453), pMDLg/pRRE (Addgene #12251) ...
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bioRxiv - Cancer Biology 2024Quote: LentiCRISPRv2 was purchased from Addgene (plasmid #98290). sgRNAs targeting PRMT1 ...
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bioRxiv - Cell Biology 2024Quote: pX458 (Addgene 48138) is suitable for both expression of SpCas9 and sgRNA ...
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bioRxiv - Cell Biology 2024Quote: ... GTS-mTagBFP and mTFP1-Actin were respectively amplified by PCR from Addgene 206256 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells (2.5 x 105) were plated in a 6-well plate and transfected with pLenti-BLRR (Addgene # 158958), pLenti-trGluc (Addgene # 158959) ...
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bioRxiv - Cancer Biology 2024Quote: ... and pX330-gRNA (Addgene # 158973) plasmids with Lipofectamine 2000 ...
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bioRxiv - Cell Biology 2024Quote: The MPV17-HA plasmid was obtained by cloning the MPV17 CDS into the pKH3, a gift from Ian Macara (Mattingly & Macara, 1996) (Addgene plasmid # 12555 ...
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bioRxiv - Cell Biology 2024Quote: ... 200-800 bases apart were designed using the Synthego Knockout Guide Design tool (http://design.synthego.com) and cloned into pSpCas9(BB)-2A-GFP (PX458; Addgene #48138) and pSpCas9(BB)-2A-mCherry ...
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bioRxiv - Cell Biology 2024Quote: ... Alice Ting (Han et al., 2017) (Addgene plasmid # 129705; http://n2t.net/addgene:129705; RRID:Addgene_129705). All sequences were cloned in a pDONR201 donor plasmid ...
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bioRxiv - Cell Biology 2024Quote: The MPV17-HA plasmid was obtained by cloning the MPV17 CDS into the pKH3, a gift from Ian Macara (Mattingly & Macara, 1996) (Addgene plasmid # 12555; http://n2t.net/addgene:12555; RRID:Addgene_12555). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial), a gift from Adam Cohen (Werley et al., 2020) (Addgene plasmid # 163076; http://n2t.net/addgene:163076; RRID:Addgene_163076). The sequence of the construct was cloned into pKH3 plasmid for its use in transfection experiment for mammalian expression ...
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bioRxiv - Cell Biology 2024Quote: ... pTH726-CEN-RLuc/minCFLuc and pTH727-CEN-RLuc/staCFLuc were a gift from Tobias von der Haar (Addgene plasmid # 38210 and 38211). Egd1 and Egd2 overexpression plasmids are under the control of their endogenous promoters (p413-Egd1 ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was then subcloned in the lentiviral vector pCSC-SP-PW-GFP (pBOB-GFP) (Addgene) for neuron infection using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs) ...
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bioRxiv - Immunology 2024Quote: ... Site-directed mutagenesis was carried out to generate catalytically inactive CBL mutants using pENTR1a plasmids (Addgene). Mutagenesis was validated through Sanger sequencing ...
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bioRxiv - Immunology 2024Quote: ... The dual-guide sgRNA vector was constructed using a synthesized gene block containing the two guides of interest and an H1 promoter from Integrated DNA technologies (IDT) that were digested with BsmBI v2 on both ends and ligated to BsmBI v2 digested Lentiguide Puro vector (Addgene) with a second-generation tracRNA (81) ...
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bioRxiv - Immunology 2024Quote: ... gateway cloning was used to move the mutated CBL open reading frame from pENTR1a into lentiviral pDEST vectors (Addgene). For THP-1 cells ...
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bioRxiv - Immunology 2024Quote: ... constructs were cloned into a pLenti CMV Blast vector (Addgene). For CIM cells ...
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bioRxiv - Plant Biology 2024Quote: ... The MoClo Toolkit was a gift from Sylvestre Marillonnet (Addgene kit # 1000000044) (Weber et al ...
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bioRxiv - Microbiology 2024Quote: ... NJ) and pCSDest-TMPRSS2 (Addgene Plasmid # 53887) plasmid was obtained from Addgene as described previously (53) ...
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bioRxiv - Microbiology 2024Quote: ... pLenti-CMV-GFP-Puro (plasmid #17448) was obtained from Addgene. To generate pLenti-CMV-hAPN-Puro ...
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bioRxiv - Microbiology 2024Quote: ... plasmid was obtained from Addgene as described previously (53) ...
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bioRxiv - Synthetic Biology 2024Quote: ... CRISPR-Cas9 expression vectors (based on plasmid pCRCT, obtained from Addgene, plasmid #60621) targeting different locations on Ty retrotransposons were constructed and co-transformed into yeast cells with donor DNA (Bao et al. ...
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bioRxiv - Molecular Biology 2024Quote: The plasmid pEGFP-N1-Importinβ (Plasmid #106941) was procured from Addgene. This plasmid has been henceforth referred to as Importinβ-1 GFP ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Vector pDONR223-SYK for GST-tagged full-length SYK was purchased from Addgene (Plasmid # 23907). Vector pDONR221-FCER1G for vFlag-tagged full length FCER1G was purchased from DNASU (Clone ID HsCD00075915) ...
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bioRxiv - Biophysics 2024Quote: ... [14] and obtained from Addgene. For NLS-TagBFP expression (Figure 6) ...
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bioRxiv - Genomics 2024Quote: ATF4 reporter Viral particles were made from pSMALB-ATF4.5 vector (a gift from John Dick & Peter van Galen; Addgene plasmid # 155032; http://n2t.net/addgene:155032; RRID:Addgene_155032) pseudotyped with the vesicular stomatitis virus G (VSVG ...
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bioRxiv - Genomics 2024Quote: ... 20Q1 Cancer Dependency Map common essential genes (https://depmap.org/portal/download/) and (ii) 5% non-targeting control sgRNAs cloned into pJR101 (Addgene #187241). A second sgRNA library (dJR092 ...
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bioRxiv - Cancer Biology 2024Quote: ... using the 2nd generation packaging system (plasmids psPAX2, Addgene #12260 and pMD2.G, Addgene #12259) were applied to cells for 72h with polybrene (10 µg/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... LKB1 sgRNAs were cloned into lentiCRISPR v2 (Addgene #52961). Lentiviral particles were prepared by transfecting HEK293 cells with EV or sgLKB1 ...
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bioRxiv - Cancer Biology 2024Quote: ... VSV-G (Addgene #8454) and Δ8.91 using Lipofectamine 3000 (ThermoFisher) ...
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bioRxiv - Cancer Biology 2024Quote: sgRNAs targeting the STK11 locus were designed using CHOP-CHOP and cloned into pSpCas9(BB)-2A-GFP (Addgene #48138). KRAS-mutant NSCLC cell lines were transiently transfected with the plasmids and sorted for single clone formation by FACs ...
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bioRxiv - Cancer Biology 2024Quote: Truncated CPSF6 containing amino acids 1-358 (CPSF6-358) from human isoform 1 was cloned into the pCW57.1 backbone (Addgene 71782). A hemagglutinin (HA ...
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bioRxiv - Genomics 2024Quote: ... pBL-TRP1-hsvTKCO-hENT1CO and pBL-AUR1C-hsvTKCO-hENT1CO plasmids are derivatives of p403-BrdU-Inc (Addgene plasmid # 71789 ...
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bioRxiv - Genomics 2024Quote: ... and p306-BrdU-Inc (Addgene plasmid # 71792 ...
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bioRxiv - Cancer Biology 2024Quote: ... VSV-G (Addgene #8454), Gag-Pol (Addgene #14887 ...
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bioRxiv - Cancer Biology 2024Quote: ... Gag-Pol (Addgene #14887) using Lipofectamine 3000 (ThermoFisher ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Target-sequence cassettes were assembled by first cloning the tRNA insert from plasmid pCFD6 (Addgene #73915) between long oligos containing the gRNA target sequences using Q5 polymerase (New England Biolabs ...
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bioRxiv - Biochemistry 2024Quote: ... obtained from Addgene 45 or 500 ng 6x-FkhP with 500 ng pCS2 Flag-FOXC2 WT from Addgene 45 or mutant FOXC2 generated as described above ...
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bioRxiv - Biochemistry 2024Quote: ... Cells that were transfected with a plasmid alone were transfected with GFP-Mapper (Addgene 117721) or C1ORF43-FLAG (SinoBiologic ...
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bioRxiv - Developmental Biology 2024Quote: ... and pcU6_3-MS2-sgRNA (Addgene: 92394) plasmids were kindly donated by the Saurka-Spengler Lab ...
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bioRxiv - Genetics 2024Quote: ... pMXs.hKlf4 (Kruppel Like Factor 4RRID:Addgene_17219), pMXs.hc-Myc (MYC Proto-Oncogene ...
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bioRxiv - Cell Biology 2024Quote: ... pSpCas9(BB)-2A-Puro (PX459) V2.0 was a gift from Feng Zhang (Addgene plasmid # 62988 ...
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bioRxiv - Genetics 2024Quote: ... pMXs.hc-Myc (MYC Proto-Oncogene, RRID:Addgene_17220), or pMX.GFP (Cell Biolabs ...
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bioRxiv - Genetics 2024Quote: ... pMXs.hSox2 (SRY-Box Transcription Factor 2, RRID:Addgene_17218), pMXs.hKlf4 (Kruppel Like Factor 4RRID:Addgene_17219) ...
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bioRxiv - Neuroscience 2024Quote: ... the pCMV-PfV-Sapphire-IRES-tdTomato plasmid (Addgene, #116934) (Delarue et al. ...
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bioRxiv - Biochemistry 2024Quote: Stably integrated NCOA4383-522 cell lines were generated via retroviral transfection of the relevant NCOA4383-522 construct in a CMV-enhancer bearing pMRX plasmid (Addgene 84573) into NCOA4Δ HeLa cells (a gift from Prof ...
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bioRxiv - Biochemistry 2024Quote: ... A g-block gene fragment (IDT) containing the sequence for NCOA4383-522 was cloned into plasmid pDW363 (Addgene 8842) via HiFi assembly (New England Biolabs ...
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bioRxiv - Biochemistry 2024Quote: GST-tagged GATE16 was expressed from plasmid pGex-4T-2_GATE-16 (Addgene 73518; pl_JD338) in E ...