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Citations for Addgene :
4301 - 4350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... USA (pAAV5-CaMKIIa-hM4D(Gi)-mCherry and pAAV5-CaMKIIa-hM3D(Gq)-mCherry were gifted from Bryan Roth (Addgene viral prep # 50477and # 50476; pAAV-CaMKIIa-mCherry was a gift from Karl Deisseroth (Addgene plasmid # 114469). Viral constructs were used as is or diluted in saline before use ...
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bioRxiv - Neuroscience 2024Quote: ... eGTACR1 was then amplified from pAAV-CAG-DIO-NLS-mRuby3-IRES-eGTACR1-ST (a gift from Dr. Hillel Adesnik; Addgene plasmid # 109048) with a BmtI restriction site on the 5’ end and a AflII restriction site on the 3’end ...
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bioRxiv - Microbiology 2024Quote: ... and psPAX2 (Addgene, #12260) packaging plasmids using JetOptimus DNA transfection reagent (Polyplus ...
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bioRxiv - Neuroscience 2024Quote: ... Four TALENs segments were digested from an 832-plasmid library (Addgene) using BsmBI and ligated into a TALENs vector carrying a FokI endonuclease domain ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV.Dlx.DIO.TVA was constructed by inserting the hDlx promoter fragment from pAAV-VTKD2 (Addgene #170847) into the backbone pAAV-EF1a-flex-TVA (Addgene #69618) ...
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bioRxiv - Neuroscience 2024Quote: pAAV.Dlx.DIO.jGcamp8m plasmid was constructed by first flipping the MCS with BcuI with the backbone pAAV-VTKD2 (Addgene #170847)65 ...
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bioRxiv - Neuroscience 2024Quote: ... we used 2 male adult PV-cre mice injected with AAV-EF1a-DIO-tdTomato (University of North Carolina Vector Core, Chapel Hill, NC USA) and AAV-Syn-GCaMP7f (Addgene, Watertown, MA USA) and 2 male adult mice expressing Ca2+ indicator jRGECO1a in cells under the Thy1 promoter (Thy1-jRGECO1a ...
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bioRxiv - Neuroscience 2024Quote: ... (Addgene # 46296 pCAG_GPHN.FingR-eGFP-CCR5TC), was a gift from D ...
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bioRxiv - Neuroscience 2024Quote: ... and pAAV-hSyn Coff/Fon hChR2(H134R)-EYFP (Addgene plasmid # 55648; http://n2t.net/addgene:55648; RRID:Addgene_55648) were gifts from Karl Deisseroth15 ...
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bioRxiv - Neuroscience 2024Quote: ... eGFP was virally expressed in STN neurons through unilateral injection of AAV9-hSyn-eGFP (RRID:Addgene_50465 ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV-Syn-flex-PSAM4-GlyR-IRES-EGFP was a gift from Scott Sternson16 (Addgene plasmid # 119741 ...
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bioRxiv - Neuroscience 2024Quote: ... mCherry-RubiconWT (RRID: Addgene 221658), mCherry-RubiconCGHL (RRID ...
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bioRxiv - Neuroscience 2024Quote: ... mCherry-LC3 (RRID: Addgene 221656), mCherry-RubiconWT (RRID ...
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bioRxiv - Neuroscience 2024Quote: ... EGFP-N1 (RRID: Addgene 6085-1). HeLa cells were transfected with 1.5 µg of untagged human Parkin (RRID ...
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bioRxiv - Neuroscience 2024Quote: ... The following plasmids were used for transfections: EGFP-Rubicon (RRID: Addgene 221657), LAMP1-Halo (RRID ...
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bioRxiv - Neuroscience 2024Quote: ... with 1µl of AAV9-CaMKII-GCaMP6f (Addgene, #100834-AAV9, dilution 1/15) at 0.75µl.min−1 (microinjection pump ...
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bioRxiv - Neuroscience 2024Quote: ... HeLa cells were transfected with 1.5 µg of untagged human Parkin (RRID: Addgene 187897) using Lipofectamine 2000 for 18-24 hrs prior to treatment.
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bioRxiv - Neuroscience 2024Quote: ... mCherry-RubiconCGHL (RRID: Addgene 221659) and EGFP-Rab7A (RRID ...
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bioRxiv - Neuroscience 2024Quote: ... LAMP1-Halo (RRID: Addgene 221655), mCherry-LC3 (RRID ...
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bioRxiv - Neuroscience 2024Quote: ... Targeting of the transgene cassettes into an endogenous gene locus was accomplished via CRISPR/Cas9-mediated genome editing using circularized targeting vector in combination with a gene-specific guide vector (Addgene plasmid #42230). The129S6B6F1 ES cell line ...
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bioRxiv - Neuroscience 2024Quote: The following AAV vectors were purchased from Addgene (Watertown, MA, USA): AAV9-hSyn-DIO-hM4D(Gi)-mCherry (#44362) ...
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bioRxiv - Neuroscience 2024Quote: ... packaging plasmid and the pMD2.G (Addgene plasmid #12259) envelope plasmid ...
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bioRxiv - Neuroscience 2024Quote: ... HEK293T cells were triple transfected with lentivirus packaging plasmid psPAX2 and the envelope plasmid pCMV-VSV-G (Addgene plasmids #12260 and #8454) in addition to the transgene of interest using FuGene HD (Promega) ...
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bioRxiv - Neuroscience 2024Quote: ... AAV.Ef1a.DIO.EYFP (Addgene #27056-AAV5, 1×1012 vg/ml) was used as a control virus for the optogenetic real time place preference task ...
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bioRxiv - Neuroscience 2024Quote: ... the pcDNA3-deltaCKAR plasmid (Addgene #31526) was digested by HindIII and XbaI ...
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bioRxiv - Neuroscience 2024Quote: The following viruses were gifts from Karl Deisseroth: AAV5-CaMKIIa-hChR2(H134R)-EYFP (Addgene plasmid #26969); AAV5-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (#20298) ...
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bioRxiv - Neuroscience 2024Quote: ... AAV.Ef1a.DIO.hChR2(E123T/T159C)-EYFP (Addgene #35509-AAV5, 1x1012 vg/ml vg/ml) was used for patch-clamp recording and for optogenetic stimulation during the optogenetic real time place preference/avoidance task ...
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bioRxiv - Neuroscience 2024Quote: ... rgAAV.hSyn.DIO.hM4D(Gi)-mCherry (Addgene #44362-AAVrg, 1.2×1013 vg/ml) and rgAAV.hSyn.DIO.mCherry (50459-AAVrg ...
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bioRxiv - Neuroscience 2024Quote: Rats were anesthetized with isoflurane (induction: 4%; maintenance: 1–2.5%) and received either AAV8-hSyn-hM4Di-mCherry (Addgene #50475-AAV8) or AAV8-hSyn-mCherry (Addgene #114472-AAV8) ...
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bioRxiv - Neuroscience 2024Quote: ... or AAV8-hSyn-mCherry (Addgene #114472-AAV8), bilaterally into the OFC or the DH ...
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bioRxiv - Neuroscience 2024Quote: ... or rgAAV.hSyn.DIO.mCherry (1.8x1013vg/ml, 100nl/hemisphere in NAc, 50nl/hemisphere in TuS, catalog #50459-AAVrg, Addgene) as control ...
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bioRxiv - Neuroscience 2024Quote: ... AAV9-hsyn-DIO-HM4D(Gi)-mCherry and AAV9-hsyn-DIO-HM3D(Gq)-mCherry were obtained from Addgene (viral prep #50459 ...
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bioRxiv - Neuroscience 2024Quote: We obtained the following AAV vectors from Addgene (titer is shown as genome particles [gp] per ml) ...
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bioRxiv - Neuroscience 2024Quote: ... 0.85 μl of virus coding for either muscarinic-receptor-based DREADD AAV8-hSyn-hM4Di(Gi)-mCherry or control virus AAV8-hSyn-EYFP (AddGene, Cambridge, MA) was injected bilaterally into either the mPFC (AP ...
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bioRxiv - Neuroscience 2024Quote: ... and pAAV-hSyn Coff/Fon hChR2(H134R)-EYFP (Addgene plasmid # 55648 ...
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bioRxiv - Neuroscience 2024Quote: We measured dopamine release using fiber photometry and GRABDA sensors (AddGene #140554). We injected AAV9-hsyn-GRAB DA2h to drive expression of the GRAB sensor ...
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bioRxiv - Neuroscience 2024Quote: Glutamate was monitored in primary astrocytes using the fluorescent reporter for glutamate pAAV.GFAP.iGluSnFR3.v857.GPI (Addgene plasmid # 178338) which was a gift from Kaspar Podgorski (Aggarwal et al. ...
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bioRxiv - Neuroscience 2024Quote: ... and control mice in this experiment received AAV8-DIO-mCherry (Addgene #50459-AAV8, 2.2 x 1013 gc/mL). After post hoc histology (described below in the “Immunofluorescence labeling” subsection) ...
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bioRxiv - Neuroscience 2024Quote: ... we used AAV8-DIO-hM3Dq-mCherry (Addgene #44361-AAV8, 2.1 x 1013 gc/mL), and control mice in this experiment received AAV8-DIO-mCherry (Addgene #50459-AAV8 ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV-hSyn-DIO-hM3D(Gq)-mCherry was a gift from Bryan Roth (Addgene plasmid # 44361; http://n2t.net/addgene:44361; RRID:Addgene_44361). pAAV-Syn-flex-PSAM4-GlyR-IRES-EGFP was a gift from Scott Sternson16 (Addgene plasmid # 119741 ...
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bioRxiv - Neuroscience 2024Quote: ... eNpHR3.0-eYFP or ChR2(H134R)-eYFP was virally expressed in parvalbumin-expressing STN neurons in PV-Cre mice through unilateral injection of Cre-dependent AAV9-Ef1a-DIO eNpHR3.0-eYFP (RRID:Addgene_26966 ...
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bioRxiv - Neuroscience 2024Quote: ... Experiments in APP-KI mice used pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA (Addgene #61591), in which the CMV-promoter was replaced with the human Synapsin1 promoter using Gibson cloning ...
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bioRxiv - Neuroscience 2024Quote: ... HEK293T cells in 150 mm dishes were co-transfected (using Polyethylenimine) with the vector plasmid pRep2/CapPHPeB (obtained from Addgene(35), and pAd-Helper plasmid(55) ...
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bioRxiv - Neuroscience 2024Quote: ... The rabies virus construct was a gift from Thomas Jessell (Addgene #7347180) and EnvA-pseudotyped CVS-N2c(ΔG)-FlpO-mCherry was generously shared by K ...
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bioRxiv - Neuroscience 2024Quote: ... then inserting the jGCaMP8m66 fragment from pGP-AAV-syn-jGCaMP8m-WPRE (Addgene #162375) digested by EcoRI and HindIII ...
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bioRxiv - Neuroscience 2024Quote: ... into the backbone pAAV-EF1a-flex-TVA (Addgene #69618), digested by EcoRI and HindIII ...
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bioRxiv - Neuroscience 2024Quote: ... AAV9 helper (Addgene plasmid #112865, 9 μg/dish), and the AAV expression vector (12 μg/dish) ...
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bioRxiv - Neuroscience 2024Quote: ... into the backbone pAAV-VTKD2 (Addgene #170847)65 digested by EcoRI and HindIII.
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bioRxiv - Neuroscience 2024Quote: ... a gift from Karl Deisseroth (Addgene plasmid # 27056). YFP was substituted for GFP in this construct ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV-FLEX-GFP (Addgene, #28304) was injected by retro-orbital sinus injection to label PV cells in Gabrd floxed::PVCre mice or PVCre littermates (Lu et al. ...