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Citations for Addgene :
4251 - 4300 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... which was a gift from Tilmann Weber (Addgene plasmid #125687; http://n2t.net/addgene:125687; RRID:Addgene_125687) (49) ...
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bioRxiv - Microbiology 2024Quote: ... which was a gift from Tilmann Weber (Addgene plasmid #125687 ...
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bioRxiv - Microbiology 2024Quote: ... pXPR_011 plasmid was a gift from John Doench & David Root [34] (Addgene, #59702). Transduced cells were transferred to 6 well plates on day 3 post transduction and treated with puromycin ...
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bioRxiv - Microbiology 2024Quote: ... a VSV-GFtail plasmid was made by replacing G tail (G protein 490-511aa) with SeV F tail (F protein 524-565aa) using plasmid pMD2.G (Addgene, #12259), then the pSL1180-rSeVCGFP-ΔFHN+GFtail full-length plasmid was made by cloning GFtail and deleting SeV F and HN gene from pSL1180-rSeVCeGFP through PCR and In-Fusion cloning (TaKaRA Bio ...
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bioRxiv - Microbiology 2024Quote: Lentiviruses for transduction were generated by co-transfecting a plasmid expressing the gene of interest or sgRNA together with the lentivirus packaging plasmids psPAX2 (Addgene, #12260) and pMD2.G (Addgene ...
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bioRxiv - Microbiology 2024Quote: ... and pMD2.G (Addgene, #12259) into Lenti-293T cells using a TransIT-Lenti Transfection Reagent (Mirus Bio ...
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bioRxiv - Microbiology 2024Quote: A549-Cas9 stable cells were transduced at a low MOI (∼0.3) with Human CRISPR Knockout Pooled Library Brunello (Addgene, #73178) [35] ...
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bioRxiv - Microbiology 2024Quote: A549-Cas9 stable line were generated by transducing lentiCas9-Blast plasmid (Addgene, #52962) [63] into A549 wt cells followed by Blasticidin selection and single cell cloning ...
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bioRxiv - Biochemistry 2024Quote: ... 1.7/0.3ug psPAX2 (Addgene 12260) and 0.85/0.15ug pMD2.G (Addgene 12259 ...
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bioRxiv - Neuroscience 2024Quote: ... purchased from Addgene and generously provided by Douglas Kim & the GENIE Project (RRID: Addgene_100838), was diluted in phosphate-buffered saline (1:3 ...
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bioRxiv - Neuroscience 2024Quote: ... purchased from Addgene and generously provided by Douglas Kim & the GENIE Project (RRID: Addgene_100838) into the anterior part of the EPd (AP ...
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bioRxiv - Neuroscience 2024Quote: ... purchased from Addgene and generously provided by Douglas Kim & the GENIE Project (RRID ...
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bioRxiv - Neuroscience 2024Quote: ... the same volume of EnvA G-deleted Rabies-EGFP virus (titer: 4.89E+09 TU/ml, purchased from Salk Institute viral vector core, a gift from Edward Callaway; RRID:Addgene_32635; Osakada et al., 2011) was injected into the same location with a 5 degree tilted angle ...
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bioRxiv - Neuroscience 2024Quote: ... AAV9.hSyn.GRAB_DA2h (1 x 1013GC/mL, Addgene) was used to detect dopamine (Sun et al. ...
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bioRxiv - Neuroscience 2024Quote: ... AAV9.CamKII.GCaMP6f (Addgene #100834-AAV9), pLV.PKG.GFP (Addgene #12252-LV ...
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bioRxiv - Neuroscience 2024Quote: ... are transfected with Dendra2-EB1 (Addgene Plasmid #57714) via lipofectamine DIV15-17 ...
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bioRxiv - Neuroscience 2024Quote: ... The pdDronpa-CDK5 (pLL3.7m-psCDK5, Addgene #89365) construct was used as a control for optimizing pdDronpa association and dissociation.
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bioRxiv - Biochemistry 2024Quote: ... was subcloned into the pET His 6 glutathione-S-transferase (GST) TEV LIC cloning vector (2G-T, obtained from Scotta Gradia, Addgene plasmid #29707) and transformed into BL21 (DE3 ...
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bioRxiv - Neuroscience 2024Quote: ... we used the adeno-associated viral vector AAV8-Syn-FLEX-jGCaMP7f-WPRE (Dr. Douglas Kim; Addgene, Cat.# 104492-AAV8) for Cre-recombinase-mediated expression of the calcium sensor protein GCaMP7f (Dana et al. ...
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bioRxiv - Neuroscience 2024Quote: ... an adenoassociated virus (AAV) expressing tdTomato under the PVIN specific S5E2 enhancer (AAV9-S5E2-tdTomato, Addgene 135630-AAV9) was injected in mice using coordinates ...
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bioRxiv - Neuroscience 2024Quote: ... 2.) piRES-puro vector (Addgene Cat# 25728) with EcoRI and NotI sites for site-directed mutagenesis experiments.
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Genetically encoded biosensor for fluorescence lifetime imaging of PTEN dynamics in the intact brainbioRxiv - Neuroscience 2024Quote: ... mEGFP followed by a linker of SGLRSA were fused to the N terminus of the PTEN coding sequence (a gift from Jose Esteban, Addgene plasmid # 110181). On the C terminus of PTEN ...
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Genetically encoded biosensor for fluorescence lifetime imaging of PTEN dynamics in the intact brainbioRxiv - Neuroscience 2024Quote: ... we used the PX330 backbone (a gift from Feng Zhang, Addgene plasmid 42230). We introduced gRNA sequences according to target gene (5’-3’) ...
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bioRxiv - Neuroscience 2024Quote: ... An HA-tagged N2cG and mutated TVA66T70,71 were inserted into both the Ai9 (Addgene plasmid #22799) and Ai65 (Addgene plasmid #61577 ...
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bioRxiv - Neuroscience 2024Quote: ... AAV2retro-CAG-FLEX-tdTomato (Addgene 28306-AAVrg) was injected into spinal segments C5 to C8 of Pet1-cre mice (8 weeks) ...
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bioRxiv - Neuroscience 2024Quote: AAV9-hsyn-GRAB_5-HT1.0 (Cat# 140552-AAV9) and AAV1-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] (Cat# 84445-AAV1) were purchased from Addgene and used for intracranial injections.
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bioRxiv - Neuroscience 2024Quote: ... miR7 (Addgene plasmid RRID: Addgene_216392) (21) ...
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bioRxiv - Neuroscience 2024Quote: ... miR5 (Addgene plasmid RRID: Addgene _216391), miR7 (Addgene plasmid RRID ...
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bioRxiv - Neuroscience 2024Quote: ... miR5 (Addgene plasmid RRID: Addgene _216391), miR7 (Addgene plasmid RRID ...
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bioRxiv - Biochemistry 2024Quote: ... and 0.85/0.15ug pMD2.G (Addgene 12259) using PEI (PolySciences) ...
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bioRxiv - Neuroscience 2024Quote: ... AAVrg-PGK-Cre (Addgene), AAV-2/1/CAG-Flex-EGFP (Addgene) ...
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bioRxiv - Neuroscience 2024Quote: ... pENN.AAV.hsyn.Cre.WPRE.hGH (AAV1, Addgene), pOOTC1032 – pAAV-EF1a-Nuc-flox(mCherry)-EGFP and Red Retrobeads™ IX (LumaFluor) ...
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bioRxiv - Neuroscience 2024Quote: We used the following viruses and tracers: AAV2/9-syn-jGCaMP8s-WPRE (Addgene), AAVrg-PGK-Cre (Addgene) ...
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bioRxiv - Neuroscience 2024Quote: ... AAV-2/1/CAG-Flex-EGFP (Addgene), pENN.AAV.hsyn.Cre.WPRE.hGH (AAV1 ...
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bioRxiv - Neuroscience 2024Quote: ... and AAVS1-Neo-M2rtTA (Addgene #60843) were nucleofected into 3 million cells by LONZA 4D-Nucleofector using program CB-150 ...
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bioRxiv - Neuroscience 2024Quote: ... and replaced with EPAC-S-H187 from Epac-S-H187 (Addgene #170348)34 ...
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bioRxiv - Neuroscience 2024Quote: ... AAV9 encoding hM4D(Gi)-mCherry (Addgene, 50475) or mCherry control (Addgene ...
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bioRxiv - Neuroscience 2024Quote: The injected retrograde viruses were pAAV-hsyn-Jaws-KGC-GFP-ER2 (Addgene; Plasmid #65014) and AVV-Syn_ChR2(H134R)-GFP (control virus ...
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bioRxiv - Neuroscience 2024Quote: ... and AVV-Syn_ChR2(H134R)-GFP (control virus; Addgene; Plasmid #58880). The retrograde tracers injected were Fluorogold 2% (Fluorochrome ...
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bioRxiv - Neuroscience 2024Quote: ... GPI-mApple (Addgene plasmid # 182868 ...
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bioRxiv - Neuroscience 2024Quote: ... AAV-DJ-EF1α::CVS-G (Produced by Stanford University Neuroscience Gene Vector and Virus Core using Addgene, plasmid #67528)54 ...
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bioRxiv - Neuroscience 2024Quote: ... A beveled glass micropipette was used to inject a combination of 3μg/μl of pCAG-GFP (Addgene; #11150; a gift from C. Cepko78) and 1μg/μl of pCAG-Kir2.1-T2A-tdTomato (Addgene ...
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bioRxiv - Neuroscience 2024Quote: ... and 1μg/μl of pCAG-Kir2.1-T2A-tdTomato (Addgene; #60598; a gift from M. Scanziani79) with 0.05% of FastGreen in the fourth ventricle of mouse embryos ...
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bioRxiv - Neuroscience 2024Quote: Viral prep AAV-FLEX-GFP was commercially acquired (Addgene; #28304-PHPeB; a gift from E. Boyden). All the other viral preps were produced as described previously91 ...
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bioRxiv - Neuroscience 2024Quote: ... was used to inject 60 nL of AAV9-syn-jGCaMP8m-WPRE (Addgene #162375) alone or mixed with PHP.eB-E6-S5E2-dTom-nlsdTom (Addgene #135630) ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... An AAV9 expressing GCaMP6f under a CaM Kinase II promoter (pENN.AAV9.CamKII.GCamp6f.WPRE.SV40, titer ≥ 1×10¹³ vg/mL, Addgene) was then injected bilaterally into the medial prefrontal cortex (mPFC) ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Mice were also injected bilaterally with virus (AAV5-CaMKIIa-eNpHR3.0-mCherry, Addgene) in the mediodorsal thalamus ...
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bioRxiv - Biochemistry 2024Quote: CRISPR-Cas9-mediated disruption of NACHO was performed using pSPCas9(BB)-2A-Puro (PX459) plasmid (Addgene) encompassing the gRNA 5’-GGCCACAATAGTTACGGTTC-3’ ...
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bioRxiv - Biochemistry 2024Quote: ... according to manufacturer protocol with 2:1 donor:sleeping beauty transposase plasmids (Addgene Plasmid #34879)80 ...
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bioRxiv - Biochemistry 2024Quote: ... Subsequent clones were created in different vectors according to the expression system following the In Vivo Assembly (IVA) cloning technique.47 MOV10 plasmid was purchased from Addgene (# 10976) and recloned into an in-house mGFP-C1 vector following the IVA technique ...