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Citations for Addgene :
3951 - 4000 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... Plasmids encoding Yap2YF and Yap3YF were constructed from pcDNA Flag-Yap1 Y357F (Addgene #18882) through site-directed mutagenesis using Neb Q5 high-fidelity polymerase (New England Biolab #M0491) ...
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bioRxiv - Developmental Biology 2024Quote: The plasmid encoding wild-type Yap was obtained from Addgene (pcDNA Flag Yap1, Addgene #18881). Plasmids encoding Yap2YF and Yap3YF were constructed from pcDNA Flag-Yap1 Y357F (Addgene #18882 ...
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bioRxiv - Developmental Biology 2024Quote: The plasmid encoding wild-type Yap was obtained from Addgene (pcDNA Flag Yap1, Addgene #18881). Plasmids encoding Yap2YF and Yap3YF were constructed from pcDNA Flag-Yap1 Y357F (Addgene #18882 ...
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bioRxiv - Developmental Biology 2024Quote: ... The gene sequences encoding N-terminally mCherry tagged Drp1 (Addgene, 49152) were inserted between the EcoRI and PstI sites of the pCDNA vector (mCherry-Drp1) ...
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bioRxiv - Developmental Biology 2024Quote: ... and peroximal targeting signal 1 (Addgene, 54520) were cloned into mCherry-bearing pcDNA6 (Golgi-mCherry and PEX-mCherry) ...
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Leveraging single cell multiomic analyses to identify factors that drive human chondrocyte cell fatebioRxiv - Developmental Biology 2024Quote: ... NFATC2 CDS was transferred into the pInducer20 lentivirus destination vector (Addgene Plasmid #4401252) using Gateway LR Clonase to generate pInducer20-NFATC2.
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bioRxiv - Developmental Biology 2024Quote: ... pHB9::EGFP (Hb9 promoter) [TJ#96] was a gift from T Jessell (Addgene plasmid # 16275 ...
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bioRxiv - Developmental Biology 2024Quote: ... pHB9::EGFP (Hb9 promoter) [TJ#96] was a gift from T Jessell (Addgene plasmid # 16275 ; http://n2t.net/addgene:16275 ; RRID:Addgene_16275) 135 ...
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bioRxiv - Genetics 2024Quote: ... counted and transduced with rtTA (Addgene 20342) and NGN2 (Addgene 99378 ...
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bioRxiv - Genetics 2024Quote: ... 250ng of Cas9 plasmid (pCAG-1BPNLS-Cas9-1BPNLS, Addgene #87108), 200ng of gRNA CVs ...
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bioRxiv - Developmental Biology 2024Quote: ... vhh-GFP4 was cut out of pUASTLOTattB_vhh-GFP4::Vkg::mCherry (Addgene #163929, (Harmansa et al., 2017) and anti-HA_fb was integrated ...
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bioRxiv - Evolutionary Biology 2024Quote: ... guide RNAs targeting PHO5 were designed using Benchling and cloned into bRA89 (AddGene 100950), a plasmid that contains the Cas9 protein and gRNA scaffold (Anand ...
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bioRxiv - Evolutionary Biology 2024Quote: ... EF06R was a gift from Eline Luning Prak (#42940, Addgene) (53) ...
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bioRxiv - Developmental Biology 2024Quote: ... or pUASTLOTattB_mCherry::CD8::vhh-GFP4 (Addgene #163930, (Harmansa et al., 2017), respectively ...
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bioRxiv - Developmental Biology 2024Quote: ... pUASTLOTattB_GrabHAExt and pUASTLOTattB-GrabHAInt were generated by replacing vhh-GFP4 of pUASTLOTattB_vhh-GFP4::CD8::mCherry (Addgene #163917, (Harmansa et al., 2015) or pUASTLOTattB_mCherry::CD8::vhh-GFP4 (Addgene #163930 ...
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bioRxiv - Cell Biology 2024Quote: ... the commercial reagent of pCS2-nCas9n (Addgene, #4729) was used in this study ...
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bioRxiv - Synthetic Biology 2024Quote: ... dCas9 was originally amplified from plasmid pJMP1159 (supplied by Addgene) removing the myc tag (Peters et al. ...
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bioRxiv - Synthetic Biology 2024Quote: ... each well was transfected with 380 ng psPAX2 (Addgene Plasmid #12260), 140 ng pMD2.G (Addgene Plasmid #12259) ...
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bioRxiv - Synthetic Biology 2024Quote: ... cloned from pCE048-SiT-Cas12a (Addgene #128124), between coding sequences and pre-crRNA motif ...
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bioRxiv - Synthetic Biology 2024Quote: ... hgRNA-E21_pLKO-Hyg (Addgene Plasmid #100563), and hgRNA-A21_pLKO-Hyg (Addgene Plasmid # 100559 ...
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bioRxiv - Synthetic Biology 2024Quote: ... hgRNA-D21_pLKO-Hyg (Addgene Plasmid #100562), hgRNA-E21_pLKO-Hyg (Addgene Plasmid #100563) ...
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bioRxiv - Synthetic Biology 2024Quote: ... and then merged it into the 3’UTR of eGFP expression cascade in LPutopia-7 (Addgene #199212) plasmid ...
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bioRxiv - Synthetic Biology 2024Quote: ... in the previous cloned pUt-mNF-GFP RMCE plasmid28 (Addgene #199220). Then MONARCH 1.0 circuit was established by inserting pre-crRNA motif (with GFP-targeting crRNA ...
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bioRxiv - Synthetic Biology 2024Quote: ... and hgRNA-A21_pLKO-Hyg (Addgene Plasmid # 100559) were ordered from Addgene6 ...
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bioRxiv - Synthetic Biology 2024Quote: ... pcDNA-Cas9-T2A-TdT (Addgene Plasmid #126424) and pcDNA-Cas9-T2A-STOP-TdT (Addgene Plasmid #126425 ...
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bioRxiv - Synthetic Biology 2024Quote: The AAVS1-targeting eSpCas9 vector (Addgene #199213) was constructed in the previous study28 ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 480 ng of pLKO (Addgene Pladmid #10878) transfer plasmid using 10 μL Lipofectamine 2000 (Thermo Fisher 11668 ...
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bioRxiv - Synthetic Biology 2024Quote: ... the LPutopia-8 genome-targeting vector was constructed based on the earlier version LPutopia-7 (Addgene #199212) assembled in the previous studies28 ...
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bioRxiv - Synthetic Biology 2024Quote: ... we first obtained the CoV-2 genomic fragment from pSLQ5080_pHR_PGK_sfGFP_CoV-F2 (Addgene #155304) and then merged it into the 3’UTR of eGFP expression cascade in LPutopia-7 (Addgene #199212 ...
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bioRxiv - Synthetic Biology 2024Quote: ... we took the template from pSLQ5429_pUC_hU6-crScaffold_EF1α-BFPv3 (Addgene #155306) and cloned in individual crRNAs using restriction-ligation method ...
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bioRxiv - Synthetic Biology 2024Quote: ... The miR-30a scaffold was PCR amplified from Addgene vector #25748 ...
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bioRxiv - Plant Biology 2024Quote: ... the CDS of AtPHF1 and AtPHO1 were amplified by PCR and subcloned into UBQ10:sXVE: S10-(MCS) (Addgene plasmid # 108177 ...
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bioRxiv - Plant Biology 2024Quote: ... the CDS of AtPHT1;1 and AtPHO2 were amplified by PCR and subcloned into UBQ10:sXVE: (MCS)-S11 (Addgene plasmid # 108179 ...
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ATP-dependent citrate lyase Drives Left Ventricular Dysfunction by Metabolic Remodeling of the HeartbioRxiv - Molecular Biology 2024Quote: ... The first gRNA was cloned into the 1179_pAAV-U6-BbsI-gRNA-CB-EmGFP backbone (Plasmid#89060, Addgene, Watertown, MA; RRID:Addgene_89060) using BbsI cloning sites ...
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bioRxiv - Molecular Biology 2024Quote: ... before being cloned into the BsmBI site (vectors had been modified to contain this site) of either the constitutive lentiviral vector FUGW (Addgene #14883) or the doxycycline-inducible vector FgH1tUTG (Addgene #70183) ...
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bioRxiv - Molecular Biology 2024Quote: ... pLVX-ATF4 mScarlet NLS (Addgene plasmid # 115969). Lentivectors expressing shRNA targeting murine Fbn1 ...
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bioRxiv - Molecular Biology 2024Quote: The dCas9a-SAM-compatible Cd19-targeting sgRNA vector was generated by first modifying the lenti sgRNA(MS2)_puro optimized backbone (Addgene #73797) to remove the puromycin selection sequence and instead incorporate a BFP marker ...
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bioRxiv - Molecular Biology 2024Quote: ... or the doxycycline-inducible vector FgH1tUTG (Addgene #70183). The 4-tandem-guide array was similarly cloned into the BsmBI site of either FUGW or FgH1tUTG.
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bioRxiv - Neuroscience 2024Quote: All viral vectors were either purchased from Addgene.org ...
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bioRxiv - Neuroscience 2024Quote: AAV9-hSyn-HI.eGFP-Cre (Addgene 105540-AAV9; titer: 5.44e11 vg/mL)
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bioRxiv - Neuroscience 2024Quote: AAV9-hSyn-GFP (Addgene 50465-AAV9; titer: 1.9e11 vg/mL)
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bioRxiv - Genetics 2024Quote: ... subtilis single gene deletion library--kanamycin was acquired from Addgene (Watertown, MA, 1000000115). The parent strain was acquired from the Bacillus Genetic Stock Center (Columbus ...
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bioRxiv - Neuroscience 2024Quote: ... 0.6-0.8 uL of a 1:1 mixture of GAD1-cre and mCherry virus (AAV8-hSyn-DIO-mCherry, ≥ 1×101 3 vg/mL; Addgene, Watertown, MA, USA) was injected bilaterally into VP ...
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bioRxiv - Evolutionary Biology 2024Quote: ... mouse RIPK1 (Addgene #115341), and mouse RIPK3 (Addgene #78805 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The protocol for the molecular cloning of this plasmid was adapted from Feng Zhang (Sanjana et al., 2014) using the transfer plasmid pLB-Cas9 (gift from Feng Zhang, Addgene plasmid # 52962). We designed the gRNA target sequence using the web tool CHOPCHOP (Labun et al. ...
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bioRxiv - Neuroscience 2024Quote: ... Gq DREADD virus (n=23, 12 males, 11 females: AAV8-hSyn-DIO-hM3Dq-mCherry, ≥ 4×101 2 vg/mL, Addgene; Watertown, MA, USA) was mixed with GAD1-cre to express excitatory designer receptors in VP GABA neurons ...
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bioRxiv - Neuroscience 2024Quote: ... or mCherry alone as control (AAV5-hSyn1-DIO- mCherry , Addgene). Coordinates were relative to Bregma according to the Franklin and Paxinos atlas third edition ...
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bioRxiv - Molecular Biology 2024Quote: ... obtained from Shinya Yamanaka (Addgene plasmid # 13459 ...
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bioRxiv - Neuroscience 2024Quote: ... and AAV9 ef1a-flex-Tdtomato viral vectors were purchased from the Canadian Neurophotonics Platform Viral Vector Core Facility (RRID:https://identifiers.org/RRID/RRID:SCR_016477) and AAVrg-flex- Tdtomato was purchased from Addgene (viral prep: 28306-AAVrg). Retrogradely infecting AAVs were used for brain injections while AAV9 was used for intravitreal injections.
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bioRxiv - Neuroscience 2024Quote: ... and AAV-SYN-DIO-GFP-IRES-tTA (Addgene #85006) in a 1:50 ratio in the barrel cortex ...