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Citations for Addgene :
3951 - 4000 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... VSV-G (Addgene #8454) and Δ8.91 using Lipofectamine 3000 (ThermoFisher) ...
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bioRxiv - Cancer Biology 2024Quote: ... using the 2nd generation packaging system (plasmids psPAX2, Addgene #12260 and pMD2.G, Addgene #12259) were applied to cells for 72h with polybrene (10 µg/ml ...
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bioRxiv - Molecular Biology 2024Quote: ... into a vector derived from the pLKO.1 vector (Addgene #10878) with pre-crRNAs under control of the hU6 promoter and BFP under control of the EF-1α promoter.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and GST-SYK (full-length) (Addgene Plasmid #211892) in a 6-well plate (Costar® 6-well Clear TC-treated Multiple Well Plates ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: HEK293T cells were transfected with plasmid containing FCER1G (full length)-VenusFlag (Addgene Plasmid #211899) and GST-SYK (full-length ...
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bioRxiv - Biochemistry 2024Quote: The plasmid encoding full-length human dynein-1 was graciously provided by the Andrew Carter Lab (Addgene plasmid 11903). This plasmid featured the dynein-1 heavy chain fused to an N-terminus for the His-ZZ-SNAPf tag ...
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bioRxiv - Biochemistry 2024Quote: ... roseus were assembled using the GoldenBraid 2.0 kit (Sarrion-Perdigones et al., 2013) (Addgene kit # 11000000076) (Fig ...
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bioRxiv - Cell Biology 2024Quote: ... psPAX2 (Addgene, No. 12260); psMD2.G (Addgene ...
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bioRxiv - Cell Biology 2024Quote: ... psMD2.G (Addgene, No. 12259); pLH sgRNA Chr1-telo (Dumont et al. ...
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bioRxiv - Biochemistry 2024Quote: The plasmid for expression of His-ULP1 was from Addgene (Plasmid #64697), the His-Sortase plasmid was created as previously described 4 ...
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bioRxiv - Genomics 2024Quote: ... and the plasmids were obtained from Addgene (accession number 127916).
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bioRxiv - Genomics 2024Quote: ... Guide RNA plasmids were assembled with gRNA sequences using the protocol described by (Mali et al., 2013) into (Addgene 41824)or ordered as custom synthesized plasmids containing the entire gRNA expression cassette in a minimal backbone (IDT).
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bioRxiv - Developmental Biology 2024Quote: ... Both the CAG-Cas9-2A-Citrine (Addgene: 92393) and pcU6_3-MS2-sgRNA (Addgene ...
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bioRxiv - Genetics 2024Quote: ... along with 3 µg of pMXs.hOct4 (Octamer-Binding Protein 4, RRID:Addgene_17217), pMXs.hSox2 (SRY-Box Transcription Factor 2 ...
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bioRxiv - Biochemistry 2024Quote: HEK293FT cell were transiently co-transfected with the fluorescent citrate sensor Citron (CMV-Citron1, AddGene #134303 (Zhao et al., 2020)) and either pcDNA3.1 carrying the WT or mutant genes T227M ...
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bioRxiv - Biochemistry 2024Quote: ... and 2.768 μg of pVSV-G (Addgene #138479) into lentiviral particles with 50 μL of Lipofectamine 2000 on 80% confluent HEK-293T cells in 6 mL of medium ...
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bioRxiv - Biochemistry 2024Quote: To make plasmid-based R-loops 2µg of pUC19-Mu-switch-R-loop plasmid (Addgene: 134899) was incubated in a final reaction volume of 200µl containing 1x T7 polymerase reaction buffer (NEB) ...
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bioRxiv - Biochemistry 2024Quote: ... FOX templates were obtained from Addgene 45 and FOX-DBD DNA sequences were ligated and cloned into the pGEX-6P2 using Gibson Assembly Master Mix (NEB ...
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bioRxiv - Biochemistry 2024Quote: Mutations of phosphorylation sites within the SR region were generated via site-directed mutagenesis based on either the tag-free SARS-CoV-2 Nucleocapsid protein plasmid (Addgene, #177937) or the Strep-tagged SARS-CoV-2 Nucleocapsid protein plasmid (Dr ...
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bioRxiv - Biochemistry 2024Quote: ... and CLN5 were deleted in HEK293 cells by CRISPR/Cas9 using the pSpCas9(BB)-2A-Puro (PX459) V2.0 vector (Addgene, plasmid #62988) and two to three sgRNAs per gene (Table S1) ...
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bioRxiv - Biochemistry 2024Quote: ... A recombinant expression construct encoding the Haspin kinase domain (GSG2; Uniprot Q8TF76; residues 465-798) was purchased from AddGene (AddGene #38915).
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bioRxiv - Biochemistry 2024Quote: Cysteine-free eGFP was amplified from ss-cfSGFP2 (Addgene #37535). PCR products were inserted into pBH4 between BamHI and NotI restriction sites using Gibson assembly.
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bioRxiv - Biochemistry 2024Quote: ... lentiviral expression constructs were previously established in pINDUCER20 (a gift from Stephen Elledge (Addgene plasmid # 44012)85 ...
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bioRxiv - Bioengineering 2024Quote: ... YAP1 (S6A) - V5 in pLX304 (Addgene plasmid # 42562 ...
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bioRxiv - Bioengineering 2024Quote: ... SaKKH-BE3 (Addgene #85170) [32] ...
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bioRxiv - Biochemistry 2024Quote: ... ETV5_pet28a was a gift from Peter Hollenhorst (Addgene plasmid # 131639)52.
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bioRxiv - Bioengineering 2024Quote: ... and 7.5 μg of psPAX2 (Addgene, #12260) into Lenti-X™ 293T cells with 50 μL of Lipofectamine 3000 and 42 μL P3000 Enhancer reagents (ThermoFisher ...
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bioRxiv - Biochemistry 2024Quote: ... into the pLew100v5-HYG expression vector (Addgene, deposited by the George Cross lab ...
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bioRxiv - Bioengineering 2024Quote: CODE gene fragments were either obtained from Addgene or synthesized by Twist Biosciences ...
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bioRxiv - Biochemistry 2024Quote: ... which was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 17451)87 ...
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bioRxiv - Biochemistry 2024Quote: ... pCDFDuet-MKK6-EE was a gift from Kevin Janes (Addgene plasmid # 82718 ; http://n2t.net/addgene:82718 ; RRID:Addgene_82718). The cloning sequence was inserted into a pET47b vector with an N-terminal 6-His tag ...
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bioRxiv - Biochemistry 2024Quote: ... pcDNA3-CD14 was a gift from Doug Golenbock (Addgene plasmid # 13645 ...
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bioRxiv - Biochemistry 2024Quote: ... hTLR4 was a gift from Ruslan Medzhitov (Addgene plasmid # 13086; http://n2t.net/addgene:13086; RRID:Addgene_13086). hMD-2_pcDNA3.1+ was purchased from Genscript (LY96_OHu26610C_pcDNA3.1(+)) ...
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bioRxiv - Biochemistry 2024Quote: ... pcDNA3-CD14 was a gift from Doug Golenbock (Addgene plasmid # 13645 ...
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bioRxiv - Biochemistry 2024Quote: ... pcDNA3-CD14 was a gift from Doug Golenbock (Addgene plasmid # 13645; http://n2t.net/addgene:13645; RRID:Addgene_13645). To measure NF-κB activity ...
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bioRxiv - Biochemistry 2024Quote: ... pGL3-ELAM-luc was a gift from Doug Golenbock (Addgene plasmid # 13029; http://n2t.net/addgene:13029; RRID:Addgene_13029). pRL-TK was purchased from Promega ...
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bioRxiv - Biochemistry 2024Quote: ... The DIvA cells were plated 24 h before experiments onto 8 well ibidi glass bottom dishes and transiently transfected with eGFP-53BP1 (Fradet-Turcotte A, 2013) (Addgene #60813) via use of Lipofectamine 3000 according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: The inserts were assembled into a vector for viral transduction (pLenti, Addgene #73582). Plasmids were digested with ClaI (New England Biolabs ...
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bioRxiv - Biophysics 2024Quote: ... namely psPAX2 (plasmid #12260 Addgene) and pMD2.G (plasmid #12259 Addgene).
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bioRxiv - Biochemistry 2024Quote: NSUN2-depleted cell lines were generated by cloning NSUN2-targeting single guide RNA sequences into the pSpCas9(BB)−2A-Puro (PX459) V2.0 plasmid (Addgene plasmid no ...
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bioRxiv - Biochemistry 2024Quote: ... The ALYREF and YBX1 portions were amplified using pCMV-APOBEC1-YTH (Addgene plasmid no ...
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bioRxiv - Neuroscience 2024Quote: ... with the cap6 gene in pRepCap6 (gifted from David Russell [Addgene plasmid # 110770; http://n2t.net/addgene:110770; RRID:Addgene_110770]). These gene engineering experiments were approved by the Institutional Committee of Gunma University (approval No ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV2/5 (gifted from Melina Fan [Addgene plasmid # 104964 ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV2/5 (gifted from Melina Fan [Addgene plasmid # 104964; http://n2t.net/addgene:104964; RRID:Addgene_104964]), pAAV2/7 (gifted from James M ...
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bioRxiv - Neuroscience 2024Quote: ... and pAAV2/AAVrh10 (gifted from James M. Wilson [Addgene plasmid # 112866; http://n2t.net/addgene:112866; RRID:Addgene_112866]) were obtained from Addgene ...
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bioRxiv - Neuroscience 2024Quote: ... The oligodendrocyte-specific mouse-derived MBP promoter was amplified from the pAAV-MBP-2xNLS-tdTomato gift from Viviana Gradinaru (Addgene plasmid # 104054 ...
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bioRxiv - Neuroscience 2024Quote: ... University of Memphis) and packaging plasmids psPAX2 and pMD2.G (gift from Didier Trono (Addgene plasmid # 12260 and 12259 ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV2/8 (gifted from James M. Wilson [Addgene plasmid # 112864 ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV2/7 (gifted from James M. Wilson [Addgene plasmid # 112863; http://n2t.net/addgene:112863; RRID:Addgene_112863]), pAAV2/8 (gifted from James M ...
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bioRxiv - Neuroscience 2024Quote: ... The astrocyte-specific human-derived GFA(ABC1D) promoter from the pZac2.1-gfaABC1D-cyto-GCaMP6f gifted from Baljit Khakh (Addgene plasmid # 52925 ...