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Citations for Addgene :
4001 - 4050 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... promoter from the pZac2.1-gfaABC1D-cyto-GCaMP6f gifted from Baljit Khakh (Addgene plasmid # 52925; http://n2t.net/addgene:52925; RRID:Addgene_52925) was amplified by KOD One PCR Master Mix (KMM-201 ...
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bioRxiv - Neuroscience 2024Quote: ... University of Memphis) and packaging plasmids psPAX2 and pMD2.G (gift from Didier Trono (Addgene plasmid # 12260 and 12259; RRID:Addgene_12260 and 12259)) ...
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bioRxiv - Neuroscience 2024Quote: ... mEmerald-ER3 (Addgene #54082), mTagBFP2-CAAX2 (synthesized with gBlock) ...
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bioRxiv - Neuroscience 2024Quote: ... We synthesized an AAV knock-in template vector (pAAV KI Cloning Vector_hSyn mTagBFP2-CAAX2) using pAAV hSyn intron Psam4GlyR m3m4 HA (Addgene, plasmid #196041) as backbone containing ...
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bioRxiv - Neuroscience 2024Quote: ... fused to the plasma membrane targeting sequence CAAX2 (Addgene plasmid #162247, eMagAF-EGFP-PM). This construct was synthesized by GenScript ...
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bioRxiv - Neuroscience 2024Quote: ... a 448 bp human synapsin-1 promoter element driving the neuronal specific expression of 4) the fluorescent protein mTagBFP2 (Addgene plasmid #191566 pLKO.1-TRC mTagBFP2) fused to the plasma membrane targeting sequence CAAX2 (Addgene plasmid #162247 ...
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bioRxiv - Neuroscience 2024Quote: ... pORANGE Tubb3-GFP KI was obtained from Addgene (Addgene plasmid #131497). The AAV vector pAAV hSyn INTRON mCherry-Jph3 WPRE ...
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bioRxiv - Neuroscience 2024Quote: ... 20172 (Addgene #86919). The post-synaptic marker PSD95-mCherry is described in Hanus et al. ...
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bioRxiv - Neuroscience 2024Quote: ... a VSV-G envelope-expressing plasmid (pMD2.G, Addgene 12259) and SIV_Gag_Pol and SIV_Rev plasmids (these two constructs will be available in Addgene upon publication of this manuscript ...
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bioRxiv - Neuroscience 2024Quote: ... and SIV_Gag_Pol and SIV_Rev plasmids (these two constructs will be available in Addgene upon publication of this manuscript) were co-transfected ...
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bioRxiv - Neuroscience 2024Quote: ... pORANGE Tubb3-GFP KI was obtained from Addgene (Addgene plasmid #131497). The AAV vector pAAV hSyn INTRON mCherry-Jph3 WPRE ...
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bioRxiv - Neuroscience 2024Quote: ... HaloTag-STIM1 (synthesized with gBlock) at BamHI and HindIII sites of pAAV Syn intron Psam4GlyR m3m4 HA (a gift from Scott Sternson, Addgene #196041). pAAV hSyn INTRON mEmerald-STIM1 was generated subcloning SS-mEmerald-linker (synthesized with gBlock ...
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bioRxiv - Neuroscience 2024Quote: ... We replaced YFP with HaloTag and mScarletI (from Addgene #85065) and subcloned the full cORF in AAV backbone at SpeI and HindIII sites to generate pAAV hSyn INTRON HaloTag-STIM2 WPRE and pAAV hSyn INTRON mScarlet-STIM2 WPRE ...
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bioRxiv - Microbiology 2024Quote: ... BHK-21/WI-2 culture supernatants were filtered (0.22 μm) and used to inoculate 293T cells that had been transfected 24 h previously with pMD2.G (Addgene 12259). At 24 h post-inoculation ...
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bioRxiv - Microbiology 2024Quote: ... BHK-21/WI-2 cells were infected with vTF7-3 for 45 min and then transfected with pVSV-EGFP-dG (Addgene 31842) or pVSV-FLuc-dG and pBS vectors encoding the N ...
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bioRxiv - Cell Biology 2024Quote: ... the lentiviral GFP expression vector (pLenti CMV GFP Puro; Addgene, plasmid 17448), and the plasmids encoding GFP (Clontech ...
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bioRxiv - Neuroscience 2024Quote: ... and pMD2-G (Gift from Didier Trono, Addgene #12260) into HEK293T cells and subsequent media collections ...
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bioRxiv - Neuroscience 2024Quote: ... Next the pL302-HA-SUMO2 plasmid was packaged into lentivirus by co-transfection of psPAX2 (Gift from Didier Trono, Addgene #12259), and pMD2-G (Gift from Didier Trono ...
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bioRxiv - Neuroscience 2024Quote: ... STIM2 construct design was based on SP-YFP-STM2(15-746) (Addgene #18862). We replaced YFP with HaloTag and mScarletI (from Addgene #85065 ...
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bioRxiv - Neuroscience 2024Quote: ... HindIII and XhoI sites where it is possible to subclone the donor sequence containing the (fluorescent) tag (the template for this region was the Addgene plasmid #131497, pORANGE Tubb3-GFP KI); 3 ...
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bioRxiv - Neuroscience 2024Quote: ... HA-SUMO2 was subcloned from pcDNA-HA-SUMO2 (gift from Guy Salvesen, Addgene #48967) and inserted into the pL302 lentiviral backbone (gift from Jacqueline Burré and Thomas Südhof at Stanford) ...
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bioRxiv - Neuroscience 2024Quote: ... and Slc17a6 knockout experiments (AAV1-CMV-FLEX-SaCas9-sgSlc17a6 (Addgene #124847), AAV1-CMV-FLEX-SaCas9-sgROSA26 gift from Dr Larry Zweifel and AAV8-Ef1a-DIO-oChief-tdTomato (Addgene# 51094) ...
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bioRxiv - Neuroscience 2024Quote: ... was used to inject a total of 800 nL of a 2:18 mixture of AAV-syn-jGCaMP7s-WPRE (Addgene: 104487-AAV1) and AAV-syn-FLEX-rc[ChrimsonR-tdTomato] (Addgene ...
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Recruitment of the m6A/Am demethylase FTO to target RNAs by the telomeric zinc finger protein ZBTB48bioRxiv - Molecular Biology 2024Quote: ... which was kindly provided by the Moffat Lab (Addgene plasmid #52961). Guide RNA sequences from the Toronto Knockout version 3 (TKOv3 ...
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bioRxiv - Plant Biology 2024Quote: ... with a double 35S promoter (pICH51277; Addgene catalog # 50268) and the same 35S terminator ...
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bioRxiv - Cell Biology 2024Quote: ... the pOPINE GFP nanobody (Addgene, plasmid 49172 [53]), non-tagged OPTN1-577 (Origene ...
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bioRxiv - Plant Biology 2024Quote: ... Plasmids pHEE401E and pCBC-DT1T2 were gifted by Qi-Jun Chen (Wang et□al.□2015, Addgene plasmid #71287 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and mouse RIPK3 (Addgene #78805) were cloned into apcDNA5/FRT/TO backbone (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: ... pcDNA3-EGFP-Rac1-Q61L was a gift from Gary Bokoch (Addgene plasmid # 12981 ...
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bioRxiv - Biochemistry 2024Quote: ... ASS1 and SLC7A5 cDNA in the vectors plX304 (Addgene #25890) and plX307 (#41392) ...
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bioRxiv - Cell Biology 2024Quote: ... sgRNAs were inserted into the BbsI site of the pX330 plasmid (pX330-U6-Chimeric_BB-CBh-hSpCas9 was a gift from Feng Zhang, Addgene plasmid # 42230) 49 ...
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bioRxiv - Cancer Biology 2024Quote: ... The M5 or SS1 CAR [36,37] was inserted into the backbone of the pSLCAR-CD19-BBz plasmid (Addgene 135992) by standard molecular cloning techniques using Gibson HiFi DNA assembly (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... pDule2-pCNF was a gift from Ryan Mehl (Addgene plasmid # 85495; http://n2t.net/addgene:85495; RRID: Addgene_85495).
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bioRxiv - Biochemistry 2024Quote: ... pDule2-pCNF was a gift from Ryan Mehl (Addgene plasmid # 85495 ...
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bioRxiv - Cancer Biology 2024Quote: ... mycBioID2 was amplified from mycBioID2-pBABE-puro (Addgene, 80900), CASP3-mycBioID2 and mycBioID2 – CASP3 were amplified from the previous developed pcDNA3 vectors using primers listed in table S1 ...
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bioRxiv - Biophysics 2024Quote: ... with 0.75 µg GFP-KASH2 vector (Addgene plasmid no. 187017) and 0.25 µg PiggyBac transposon vector ...
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bioRxiv - Cancer Biology 2024Quote: ... and psPAX2 (Addgene, Cat. 12260, RRID: Addgene_12260) at a 2:1:1 ratio using Lipofectamine 3000 transfection reagents (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: LentiGuide-Puro (Addgene, Cat. 52963, RRID: Addgene_52963) and lentiCas9-Blast (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: ... Lentiviruses were generated by transfecting sub-confluent HEK293T cells along with the lentiviral vector and packaging vectors pCMV-VSV-G (Addgene, Cat. 8454, RRID: Addgene_8454) and psPAX2 (Addgene ...
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bioRxiv - Biophysics 2024Quote: ... GCaMP plasmid (Addgene plasmid #100843) (Chen et al. ...
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bioRxiv - Cancer Biology 2024Quote: LentiGuide-Puro (Addgene, Cat. 52963, RRID: Addgene_52963) and lentiCas9-Blast (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: ... and psPAX2 (Addgene, Cat. 12260, RRID: Addgene_12260) at a 2:1:1 ratio using Lipofectamine 3000 transfection reagents (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... Lentiviruses were generated by transfecting sub-confluent HEK293T cells along with the lentiviral vector and packaging vectors pCMV-VSV-G (Addgene, Cat. 8454, RRID: Addgene_8454) and psPAX2 (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: ... and lentiCas9-Blast (Addgene, Cat. 52962, RRID: Addgene_52962) plasmids were used to generate CDK12 and CDK9 KO cell lines ...
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bioRxiv - Cell Biology 2024Quote: Individual sgRNAs were cloned into the pLenti-CRISPR-V2 (Addgene #98293) lentivectors for CRISPR Knock-out (CRISPR-KO ...
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bioRxiv - Cell Biology 2024Quote: ... and the envelope expressing plasmid (pCMV-VSV-G, Addgene #8454). Virial supernatant was harvested 48 h post-transfection ...
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bioRxiv - Cancer Biology 2024Quote: Enzymatically dead Cas9-KRAB fusion protein (Lenti-dCas9-KRAB-blast, Addgene, #89567) was introduced into three KP LUAD cell lines by lentiviral transduction and blasticidin selection ...
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bioRxiv - Cancer Biology 2024Quote: HEK293T cells were transfected with a shuttle vector plasmid and packaging plasmids pMD2.G (Addgene, #12259) and psPAX (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: ... All sgRNAs used for validation (Supplementary Table 7) were cloned into the pLenti-sgRNA(lib)-puro vector (Addgene, #119976) through Golden Gate assembly ...
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bioRxiv - Cancer Biology 2024Quote: pLenti-ABEmax-P2A-EGFP expression plasmid was constructed by cloning ABEmax_P2A_EGFP sequence from pCMV_ABEmax_P2A_GFP (Addgene, #112101) into the lentiviral vector ...