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Citations for Addgene :
4101 - 4150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... two packaging plasmids (pMDLg/pRRE, Addgene #12251 and pRSV-Rev, Addgene #12253) and the envelope expressing plasmid (pCMV-VSV-G ...
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bioRxiv - Cell Biology 2024Quote: ... two packaging plasmids (pMDLg/pRRE, Addgene #12251 and pRSV-Rev, Addgene #12253) and the envelope expressing plasmid (pCMV-VSV-G ...
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bioRxiv - Cell Biology 2024Quote: The constructs for SF-Ch2 assays were obtain from Addgene (plasmid #82604 and #82602). Constructs were then cloned and expressed using the lentiviral system as described above ...
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bioRxiv - Cell Biology 2024Quote: The monoclonal SK-N-DZ SCLYKO cell line was transduced with Toronto KnockOut (TKO) CRISPR Library - Version 3 (Addgene, 90294) at MOI of 0.3 ...
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bioRxiv - Biophysics 2024Quote: ... two primer sets were made for cpHaloTag in pCAG-HASAP (AddGene plasmid #138325) and rTRPV1 ...
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bioRxiv - Cancer Biology 2024Quote: ... The mAID-mCherry cassette was amplified from Addgene plasmid 72830 (pMK292)45 and mAID-HaloTag was amplified from Addgene plasmid 112852102 ...
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bioRxiv - Cancer Biology 2024Quote: ... to target NCL translation start site and the targeting sequence was cloned into pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem (1/110) (Addgene#71707)103 according to standard protocols104 ...
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bioRxiv - Cancer Biology 2024Quote: ... the lentiviral packaging plasmids pVSVg (Addgene, 8454) and psPAX2 (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: The PLenti.CMV.GFP.Puro (Addgene#17488) plasmid were transduced into the SCC070 cells ...
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bioRxiv - Cancer Biology 2024Quote: ... and mycBioID2 was amplified from mycBioID2-pBABE-puro (Addgene, 80900) using primers listed in Table S1 (designed using the Takara in-Fusion Cloning Design Primer tool (https://takara.teselagen.com/#/DesignPage)) ...
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bioRxiv - Cancer Biology 2024Quote: ... CASP3 was amplified from pcDNA3-Casp3-myc (Addgene, 11813) and mycBioID2 was amplified from mycBioID2-pBABE-puro (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: ... The CASP3 gene was amplified from the CASP3 coding plasmid (Addgene, 11813) using specific primers (detailed in Table S1) ...
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bioRxiv - Biophysics 2024Quote: His-tagged TEV protease (pDZ2087, a gift from David Waugh (Addgene plasmid # 92414 ...
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bioRxiv - Molecular Biology 2024Quote: ... psPAX2 (Addgene; plasmid #12260), and MD2.G (Addgene ...
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bioRxiv - Molecular Biology 2024Quote: For overexpression and co-immunoprecipitation experiments we used the following plasmids: pCMV-NEMO-FLAG (Addgene #11970 [59]), pMTBS-sT-YFP (Addgene #32226 [60] ...
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bioRxiv - Molecular Biology 2024Quote: ... the coding sequences of p62-HA (Addgene #28027), LC3-EGFP (Addgene #11546 ...
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bioRxiv - Molecular Biology 2024Quote: ... 8 µg sgRNA/Cas9 plasmid (Addgene 62988, Supplementary table 6), 200 µl Opti-Mem (Gibco ...
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bioRxiv - Molecular Biology 2024Quote: ... the FKBPv-V5 tag was obtained from Addgene plasmid 91798 and knocked into the Mlf2 and Smarca4 loci using HDR templates containing 0.5-1 kb homology arms flanking the C-terminal stop codons ...
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bioRxiv - Neuroscience 2024Quote: ... and C1(1-29)-TurboID-V5_pCDNA3 vectors were obtained from Addgene (149414, 149415, 166971, 107173).58–60 Each was separately inserted into the pZac2.1 GfaABC1D-tdTomato vector109 obtained from Addgene (44332 ...
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bioRxiv - Molecular Biology 2024Quote: ... The Sleeping Beauty vector was co-transfected with a Sleeping Beauty Transposase (Addgene #34879) using Lipofectamine3000 (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2024Quote: ... The chosen sgRNA for METTL3 or YTHDC1 was cloned into the pCAG-eCas9-GFP-U6-gRNA plasmid (Addgene #79145), which harbored the green fluorescent protein (GFP ...
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bioRxiv - Neuroscience 2024Quote: ... Briefly, we expressed GCaMP6s (Chen et al., 2013) in neurons by microinjecting AAV1.hSyn.GCaMP6s.WPRE.SV40 (Addgene) into layer 2/3 of targeted cortical areas at P13-32 ...
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bioRxiv - Molecular Biology 2024Quote: The Zαβ domain of human ZBP1 was cloned into the pLIX403_Capture1_APOBEC vector (Addgene #183951) utilizing the gateway cloning method ...
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bioRxiv - Molecular Biology 2024Quote: Oligoes containing shRNA target sequences were cloned into pLKO.1-blast lentiviral vector (Addgene #26655). shRNA sequences used in this study are listed below.
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bioRxiv - Molecular Biology 2024Quote: ... pLIX403_APOBEC (Addgene #183901), was utilized as control experiment (APOBEC1).
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bioRxiv - Neuroscience 2024Quote: All viral vectors were either purchased from Addgene.org ...
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bioRxiv - Microbiology 2024Quote: A custom gRNA-expressing plasmid was crafted following the gRNA synthesis protocol developed by Addgene Inc. ...
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bioRxiv - Microbiology 2024Quote: The TR146 cells were transduced with lentivirus that carried the spCas9-Blast construct (Addgene, #52962) at MOI = 0.3 as described above ...
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bioRxiv - Molecular Biology 2024Quote: The inducible CASPEX expression plasmid (Addgene #97421) was stably transfected into Dbp(luc ...
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bioRxiv - Molecular Biology 2024Quote: ... The hANP32A 1-149 construct was a gift from Cynthia Wolberger (Addgene plasmid # 67241 ...
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bioRxiv - Microbiology 2024Quote: ... and pMD2.G (Cat# 12259, Addgene) at a mass ratio of 2:1.33:1 ...
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bioRxiv - Microbiology 2024Quote: ... The cDNA of interest was amplified by PCR using primers (Table S4) with overhangs that allow directional cloning into EcoRV-linearized pHIVdTomato (#21374, Addgene) using the 2X Gibson Assembly Kit (Cat # E2611S ...
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bioRxiv - Microbiology 2024Quote: ... Roughly 1,000 base pairs upstream and downstream of the desired deletion location were amplified via PCR and assembled into allelic exchange vector pIMAY (Addgene plasmid # 68939, gifted by Tim Foster) (69 ...
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bioRxiv - Molecular Biology 2024Quote: ... Single sgRNA CDSs were cloned in pLentiCRISPRv2 (Addgene plasmid 52961) to perform stable knock-outs in HEK-293T cells ...
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bioRxiv - Microbiology 2024Quote: ... Staphylococcus aureus Newman expressing GFP from a low-copy plasmid (pTRKH3-ermGFP, Addgene #27169) (Lizier et al ...
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bioRxiv - Microbiology 2024Quote: ... together with lentiviral packaging vectors pMD2.G and psPAX2 (gifts from Didier Trono, Addgene). The lentiviral particles were harvested at 48 h and filtered through 0.45 μm syringe filter unit (Millipore ...
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bioRxiv - Microbiology 2024Quote: ... pyogenes Cas9 was amplified from px330 (Addgene, cat #42230, Feng Zhang) and linked with a P2A ribosomal skipping sequence to eGFP in rSeV ...
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bioRxiv - Microbiology 2024Quote: ... purchased from Addgene, was prepared from E ...
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bioRxiv - Microbiology 2024Quote: ... Cells were transduced with the Human CRISPR Knockout Pooled Library16 (Brunello, lentiCRISPR v2, Addgene 73179-LV) at ∼MOI 0.3 with 8 µg/ml polybrene ...
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bioRxiv - Microbiology 2024Quote: ... Approximately 200 million K562-Cas9-Blast cells were transduced with each GeCKOv2 library A or B [46] (Addgene #1000000048 or #1000000049 ...
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bioRxiv - Microbiology 2024Quote: ... and SV2B K562-DCSIGN KO cell lines were generated via nucleofection with top-ranking sgRNAs (Table S4) cloned into px458 (Cat# 48138, Addgene). One million cells resuspended in 100 μL of SF Cell Line Nucleofector solution (Lonza V4XC-2012 ...
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bioRxiv - Microbiology 2024Quote: ... Pooled gRNAs were synthesized (Twist Biosciences, South San Francisco, CA) and cloned into lentiCRISPRv2 (Cat# 52961, Addgene), packaged into lentivirus as described above ...
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bioRxiv - Microbiology 2024Quote: Lentivirus was produced in HEK-293T/17 cells by co-transfection of lentiviral plasmids with psPAX2 (Cat# 12259, Addgene) and pMD2.G (Cat# 12259 ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... and pTwist-SARS-CoV-2 d18 B.1.1.529 (Addgene #179907) were kindly shared by Dr ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... the packaging plasmid psPAX2 was used along with pCMV-VSV-G encoding the VSV G glycoprotein (Addgene #8454). Depending on the desired modification ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... TMPRSS2 was subcloned into the pLJM1 lentiviral vector (Addgene #19319) by standard cloning approaches ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... psPAX-2 (Addgene #12260) was used as a packaging plasmid ...
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The HUSH epigenetic repressor complex silences PML nuclear bodies-associated HSV-1 quiescent genomesbioRxiv - Microbiology 2024Quote: ... plasmids of interest (see plasmids section) were co-transfected with psPAX.2 (Addgene #12260) and pMD2.G (Addgene #12259 ...
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bioRxiv - Microbiology 2024Quote: K562-Cas9-Blast cells (provided by Andreas Puschnik, Chan Zuckerberg Biohub, San Francisco) were generated by transduction with lentiCas9-Blast (Cat# 52962, Addgene) and selection with blasticidin as described previously [38] ...
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bioRxiv - Microbiology 2024Quote: ... mCherry-2xFYVE plasmid was obtained from Addgene (#140050). SARS-CoV-2 subgenomic ΔS-E-M replicon was provided by Dr ...