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Citations for Addgene :
3801 - 3850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... 0.83 µg of psPAX2 (Addgene plasmid # 12260), and 1.1 µg of sgRNA or shRNA plasmid ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were co-transfected with 0.56 µg of pMD2.G (Addgene plasmid # 12259), 0.83 µg of psPAX2 (Addgene plasmid # 12260) ...
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bioRxiv - Cancer Biology 2024Quote: NAMPT enhancer deletions were generated by cloning the first sgRNA targeting upstream of the enhancer into TLCV2 and the second sgRNA targeting 1kb downstream of the first sgRNA into pSpCas9(BB)-2A-GFP (PX458) (Addgene; 48138) which had Cas9 and EGFP replaced with RFP.
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bioRxiv - Cancer Biology 2024Quote: shMAFG cells were generated using pLKO.1 (Addgene; 10878) cloned with shRNA sequences (Supplementary Table ...
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bioRxiv - Cancer Biology 2024Quote: pLenti CMV V5-LUC Blast (Addgene; 21474) was used for luciferase overexpression.
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bioRxiv - Cancer Biology 2024Quote: Fn14 CRISPR KO cells were generated using TLCV2 (Addgene; 87360) cloned with two different sgRNA guides ...
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bioRxiv - Cancer Biology 2024Quote: The TWEAK overexpression construct was generated by amplifying the soluble TWEAK (sTWEAK) sequence from cDNA through PCR and this was cloned into pLJM1-EGFP (Addgene; 19319), replacing EGFP ...
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bioRxiv - Cancer Biology 2024Quote: ... NAMPT and mouse Fn14 CRISPR KD cells were generated using lentiCRISPR v2 (Addgene; 52961) cloned with two different sgRNA guides.
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bioRxiv - Cancer Biology 2024Quote: Cas9-gRNA-GFP vector (Addgene #124770) as described earlier (Giuliano et al ...
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bioRxiv - Cancer Biology 2024Quote: ... sgRNAs were cloned into the lentiGuide-Puro vector (Addgene #52963). The finished library consisted of 494 sgRNAs targeting 155 human and mouse genes ...
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bioRxiv - Cancer Biology 2024Quote: ... psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259 ...
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bioRxiv - Cancer Biology 2024Quote: The all-in-one version of the Human CRISPR knockout Pooled Library (Addgene #73179) was applied for in vitro genome-wide loss-function screen in PaTu-8988t cells23 ...
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bioRxiv - Cancer Biology 2024Quote: ... targeting a DNA sequence within the first exon of the FasL gene (5’-CTGGGCACAGAGGTTGGACA-3’) was cloned into the BsmBI restriction site of the 3rd generation LentiCRISPR.V2 (Addgene, #52961) vector ...
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bioRxiv - Cancer Biology 2024Quote: ... Experiments were performed with co-transfection of a constitutively active renilla reporter (Addgene #27163; RRID: Addgene_27163). Analysis was completed by dividing firefly activity by renilla activity.
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bioRxiv - Cancer Biology 2024Quote: ... Experiments were performed with co-transfection of a constitutively active renilla reporter (Addgene #27163; RRID: Addgene_27163). Analysis was completed by dividing firefly activity by renilla activity.
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bioRxiv - Cancer Biology 2024Quote: ... 1 μg of pMD2.G (AddGene, #12259), and 1.5 μg of psPAX2 (AddGene ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1.5 μg of psPAX2 (AddGene, #12260) mixed in 0.8 ml Opti-MEM medium (Thermo ...
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bioRxiv - Cancer Biology 2024Quote: ... DNMT1 and DNMT3B were obtained using lentiviral expression vector pLKO.1 (Addgene #10878) (shRNA sequences are listed in Supplementary Table 1 ...
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bioRxiv - Cancer Biology 2024Quote: ... The pcDNA3.1-p300 was purchased from Addgene (#23252). The wild-type AKT1 (NM_001014431.2 ...
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bioRxiv - Cancer Biology 2024Quote: ... The following guide sequences were cloned in the lentiCRISPRV2 vector that was a gift from Feng Zhang (Addgene plasmid #52961): sg BCL-xL ...
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bioRxiv - Cell Biology 2024Quote: ... the pLVX-KRT6A-mRb-IRES-Neo construct includes an mRuby2 (Addgene #40260) 99 sequence in-frame with the KRT6A sequence separated by the same linker.
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bioRxiv - Cell Biology 2024Quote: Keratin sequences were cloned by polymerase chain reaction (PCR) from pBabe-RFP1-KRT5-hygro (Addgene #58493) and pDONR22-KRT6A (DNASU clone HsCD00039474 ...
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bioRxiv - Cell Biology 2024Quote: ... the pAdDeltaF6 plasmid (Addgene #112867), and the pAAV2/8 plasmid (Addgene # 112864 ...
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bioRxiv - Cell Biology 2024Quote: ... and the pAAV2/8 plasmid (Addgene # 112864) using polyethylenimine (1 µg/µl ...
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bioRxiv - Neuroscience 2024Quote: ... full length Rab3A sequence was amplified from EGFP-Rab3A vector (Addgene plasmid # 49542) using PCR ...
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bioRxiv - Neuroscience 2024Quote: ... The EB3-Tdtomato construct was obtained from Addgene (Addgene plasmid #50708; http://n2t.net/addgene:50708; RRID:Addgene_50708). For rAAV9-syn-EGFP-Rab3A construct ...
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bioRxiv - Neuroscience 2024Quote: ... The EB3-Tdtomato construct was obtained from Addgene (Addgene plasmid #50708 ...
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bioRxiv - Neuroscience 2024Quote: ... were cloned by PCR amplification of human LGals3 and LGals8 cDNAs from the pHAGE-mKeima-LGALS3 and pHAGE-FLAG-APEX2-LGALS8 plasmids (Addgene plasmids #175780 and #175758 ...
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bioRxiv - Neuroscience 2024Quote: ... 6-week-old Slc17a7Cre mice were injected with pAAV-EF1a-DIO-tdTomato-WPRE virus (RRID:Addgene_133786 ...
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bioRxiv - Neuroscience 2024Quote: ... an oligonucleotide pair (Supplemental Table 3) was annealed and cloned into BbsI-digested pCFD3-dU6-3gRNA (Addgene #49410) as described (Port et al. ...
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bioRxiv - Neuroscience 2024Quote: ... oligonucleotide pairs (Supplemental Table 4) were used for PCR and inserted into pCFD5 (Addgene #73914) via Gibson Assembly ...
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bioRxiv - Neuroscience 2024Quote: ... sechellia UAS-SPARC2-D-CsChrimson: we digested a SPARC2-backbone vector (Addgene #133562) with SalI and inserted a CsChrimson-Venus cassette after PCR amplification from pBac(UAS-ChR2 CsChrimson,3xP3::dsRed ...
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bioRxiv - Neuroscience 2024Quote: ... sechellia nos-Cas9 (Auer et al., 2020) or co-injected with pHsp70-Cas9 (400 ng μl−1) (Addgene #45945; for D. simulans transgenesis) (Gratz et al. ...
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bioRxiv - Neuroscience 2024Quote: ... The Dsec-nSyb-ΦC31 line was generated by integration of nSyb-ΦC31 (Addgene #133868) into Dsec-attP26 (see next section).
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bioRxiv - Neuroscience 2024Quote: ... and a 2nd generation viral packaging system comprising gag/pol/tat/rev (psPAX2, Addgene, #12260) and VSV-G (pMD2.G ...
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bioRxiv - Neuroscience 2024Quote: ... and VSV-G (pMD2.G, Addgene, #12259). Cells were seeded in a 15 cm dish in 22.5 ml medium (DMEM with 4.5g/l glucose and pyruvate ...
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bioRxiv - Neuroscience 2024Quote: ... conferring resistance to puromycin (Addgene 52961). After cell transduction with the lentiviruses expressing the Cas9/gRNAs ...
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bioRxiv - Neuroscience 2024Quote: ... gRNAs were designed using the prediction software https://portals.broadinstitute.org/gpp/public/analysis-tools/sgrna-design and inserted in pX330-U6-Chimeric_BB-Cbh-hSpCas9 (gift from Feng Zhang, Addgene plasmid #42230 ...
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bioRxiv - Neuroscience 2024Quote: ... These mice were unilaterally injected with AAV2-EF1a-DIO-ChR2-eYFP (obtained from Addgene) in the left prefrontal cortex based on stereotaxic coordinates measured from bregma (AP 1.8mm ...
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bioRxiv - Neuroscience 2024Quote: ... and AAVrg-EF1a-Cre-mCherry (obtained from Addgene) was injected in the downstream target region based on stereotaxic coordinates measured from bregma (NAc ...
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bioRxiv - Neuroscience 2024Quote: ... thirteen F1 offspring were bilaterally injected with AAV2-hSyn-DIO-GqDREADD (obtained from Addgene) based on stereotaxic coordinates measured from bregma (AP -1.5mm ...
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bioRxiv - Neuroscience 2024Quote: ... AAV.9.FLEX.rev.ChR2(H134R).mCherry (optogenetics, 140 nl, Addgene, no. 18916), AAV.9.Ef1A.fDIO.EYFP (control for optogenetics ...
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bioRxiv - Neuroscience 2024Quote: ... AAV.9.Ef1A.fDIO.EYFP (control for optogenetics, 140 nl, Addgene, no55641).
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bioRxiv - Neuroscience 2024Quote: ... stGtACR2-expressing adeno-associated virus (AAV) vector (pAAV_hSyn1-SIO-stGtACR2-FusionRed, Addgene, #105677) stock solution was diluted to 5×1011 gc/ml titers ...
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bioRxiv - Molecular Biology 2024Quote: ... CMV promotor and tdTomato sequences were obtained from pMuLE ENTR CMV tdTomato L5-L2(43) (Addgene #62152), and CMV promotor for EGFP was obtained from pMuLE ENTR CMV eGFP L3-L2(43 ...
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bioRxiv - Molecular Biology 2024Quote: ... pUCmini-iCAP-PHP.eB(44) (Addgene #103005), pAdDeltaF6 (Addgene #112867) ...
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bioRxiv - Molecular Biology 2024Quote: ... pAdDeltaF6 (Addgene #112867), into HEK293T cell ...
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bioRxiv - Molecular Biology 2024Quote: ... and CMV promotor for EGFP was obtained from pMuLE ENTR CMV eGFP L3-L2(43) (Addgene #62143) by PCR amplification ...
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bioRxiv - Molecular Biology 2024Quote: ... All fragments were inserted into AAV backbone vector (pAAV-CAG-GFP, Addgene #37825) using NEB HiFi DNA Assembly Master Mix (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... and cloned into pU6-tevopreq1-GG-acceptor41 (a gift from David Liu; Addgene 174038). K562 cells stably expressing the mSc-TOSI reporter were transfected with PE3max-epegRNA (tevopreQ1 ...