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Citations for Addgene :
151 - 200 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... These parts were constructed by obtaining a template plasmid from Addgene, amplifying the desired fragment by PCR with primers that introduce the required SapI cut sites and overhangs ...
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bioRxiv - Synthetic Biology 2024Quote: ... pIB184-Km (Addgene plasmid # 90195) and pIB185 (Addgene plasmid # 90196 ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmids pIB166 (Addgene plasmid # 90189), pIB184-Km (Addgene plasmid # 90195 ...
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bioRxiv - Systems Biology 2024Quote: ... pcDNA3 VP4:VP2 (Addgene, 216787) was prepared by PCR cloning of amino acids 1–332 from pcDNA3 CVB3 into the BamHI–EcoRI sites of pcDNA3 ...
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bioRxiv - Systems Biology 2024Quote: ... 4.5 μg of psPAX2 (Addgene #12260), 1.5 μg of pMD2.G (Addgene #12259 ...
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bioRxiv - Synthetic Biology 2024Quote: ... and pIB185 (Addgene plasmid # 90196) were donated by Indranil Biswas ...
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bioRxiv - Microbiology 2024Quote: ... was procured from (Addgene 102939). The pSAM-Ec was introduced into B ...
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bioRxiv - Synthetic Biology 2024Quote: ... All WCFS1 transformations were done via electroporation following a previously documented protocol.24 WCFS1 was first transformed with helper plasmid pLH01 (Addgene Plasmid #117261) containing phase recombinases under an inducible promoter ...
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bioRxiv - Synthetic Biology 2024Quote: ... the BMP2 CDS was removed and replaced with EGFP excised from the pCAG_GFP plasmid previously purchased from Addgene (https://www.addgene.org/11150/). Novel SP fragment synthesis ...
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bioRxiv - Synthetic Biology 2024Quote: ... genome using a previously reported phage-recombinase method.23 Genetic sensor constructs were first constructed on the shuttle plasmid pTRKH2 (Addgene Plasmid #71312) and sequence verified ...
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bioRxiv - Synthetic Biology 2024Quote: ... pJFRC81-10XUAS:IVS-Syn21-GFP-p10 (hereafter pJFRC81) was a gift from Gerald Rubin (Addgene plasmid #36432 ; http://n2t.net/addgene:36432 ; RRID:Addgene_36432). The region 67 bp upstream of the start codon of the GFP CDS of pJFRC81 was used as an extended upstream homology arm to re-introduce the Syn21 5’UTR during assembly ...
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bioRxiv - Systems Biology 2024Quote: ... CYC1t and pTEF1 were both PCR amplified from BB3cK_pGAP_23*_pTEF_Cas9 (Addgene #104909) (Gassler et al. ...
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bioRxiv - Synthetic Biology 2024Quote: ... and the sgRNA for the TREE reporter system (164413) were all obtained from Addgene. To generate Ai9 reporter 293T cells ...
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bioRxiv - Synthetic Biology 2024Quote: ... pJFRC81-10XUAS:IVS-Syn21-GFP-p10 (hereafter pJFRC81) was a gift from Gerald Rubin (Addgene plasmid #36432 ...
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bioRxiv - Systems Biology 2024Quote: ... pEN_TT CVB3 A67G (Addgene, 216788) was prepared in a two-step procedure ...
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bioRxiv - Synthetic Biology 2024Quote: ... Select regions of EphA1 (excluding the extracellular regions of IM-EphA1Cry2 construct) were cloned into Cry2olig-mCherry (Addgene: #60032) using NheI and BmtI restriction sites ...
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bioRxiv - Synthetic Biology 2024Quote: ERK-KTR-mNeonGreen was subcloned from Addgene plasmid #129631 (Chavez-Abiega ...
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bioRxiv - Systems Biology 2024Quote: ... sgRNAs were cloned into the CROPseq-puro-v2 backbone (Addgene #127458). pXPR_BRD023 (lentiCRISPR v2 ...
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bioRxiv - Systems Biology 2024Quote: ... BJ1 cells were transduced with Cas9 (Addgene #96924) and selected using 10 µg/mL blasticidin for 7 days ...
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bioRxiv - Systems Biology 2024Quote: Libraries were cloned as previously described into a CROP-seq-puro-v2 (Addgene #127458) backbone ...
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bioRxiv - Neuroscience 2024Quote: ... Lentiviral particles were produced in the HEK293T cell line using psPAX2 (12260, Addgene) and pMD2.G (12259 ...
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bioRxiv - Neuroscience 2024Quote: ... cells were transduced with an inducible system Tet-pLKO-puro (21915, Addgene) encoding an shRNA targeting TARDBP sequence (TRCN0000016038 ...
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bioRxiv - Neuroscience 2024Quote: ... and pMD2.G (12259, Addgene) plasmids (both a gift from Dr ...
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bioRxiv - Neuroscience 2024Quote: ... peGFP-N1-FLAG (Addgene plasmid # 60360).
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bioRxiv - Neuroscience 2024Quote: ... into the BsmBI-digested pBPK1520 plasmid (BPK1520 was a gift from Keith Joung. Addgene#65777; http://n2t.net/addgene: 65777; RRID:Addgene_65777)138 ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli chromosome at the λ integration site by electroporating this plasmid into DH10B cells containing the helper plasmid pInt-ts (Addgene #66076) and selecting for kanamycin resistant colonies ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli DH10GFP (Addgene #109392), a strain constructed in the same way in the prior study of burden by Ceroni et al.21
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bioRxiv - Synthetic Biology 2024Quote: ... we inserted the constitutive GFP expression cassette cloned into pAH63 (Addgene #66073) into the E ...
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bioRxiv - Neuroscience 2024Quote: ... into the BsaI-digested pU6-peg-GG-acceptor (pU6-pegRNA-GG-acceptor was a gift from David Liu. Addgene #132777; http://n2t.net/addgene: 132777; RRID:Addgene_132777). Prime editing nicking guide plasmids (ngRNAs ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli BW25113 carrying pDY118A using a donor plasmid (pKAW086, Figure S3, Table S2, Addgene ID 217967) containing an editing cassette comprised of kanR flanked by regions of homology to the genome (Table S6) ...
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bioRxiv - Neuroscience 2024Quote: ... Plasmid based prime editing: 500 ng pCMV-PE2-GFP (a gift from David Liu, Addgene#132776)115 ...
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bioRxiv - Neuroscience 2024Quote: ... pegRNA plasmids for prime editing were cloned by ligating annealed oligonucleotide pairs (Supplemental table 5) into the BsaI-digested pU6-peg-GG-acceptor (pU6-pegRNA-GG-acceptor was a gift from David Liu. Addgene #132777 ...
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bioRxiv - Synthetic Biology 2024Quote: ... The editing cassette (Table S7) was amplified from pKAW066 (Figure S4, Table S2, Addgene ID 217968) using the manufacturer’s protocol for NEB Q5 Hot Start DNA Polymerase (M0494L ...
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bioRxiv - Neuroscience 2024Quote: ... AAV2/5.CaMKII.tdTomato (Neurophotonics, 661-aav5) was used to label excitatory neurons and AAV9.CaMKII.GCaMP6s.WPRE.SV40 (Addgene, 107790) was employed to image excitatory neuronal calcium activity ...
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bioRxiv - Neuroscience 2024Quote: ... 2.3 nL of AAV1-CAG-tdTomato (59462-AAV1; Addgene) were injected twice ...
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bioRxiv - Neuroscience 2024Quote: pGP-AAV-syn-jGCaMP7c variant 1513-WPRE was a gift from Douglas Kim & GENIE Project (Addgene viral prep # 105321-AAV1 ...
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bioRxiv - Neuroscience 2024Quote: ... A 603 bp long nucleotide encoding for cystatin-7 was cloned into the BamHI/EcoRI sites of pAAV-hSyn-EGFP (# 50465, Addgene) to receive the plasmid pAAV-hSyn-Cst7 ...
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bioRxiv - Neuroscience 2024Quote: ... and pAAV-hSyn1-GcaMP6s-P2A-nls-dTomato (which was a gift from Jonathan Ting [Addgene plasmid # 51084 ...
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bioRxiv - Neuroscience 2024Quote: pGP-AAV-syn-jGCaMP7c variant 1513-WPRE was a gift from Douglas Kim & GENIE Project (Addgene viral prep # 105321-AAV1; http://n2t.net/addgene:105321; RRID: Addgene_105321)54 ...
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bioRxiv - Molecular Biology 2024Quote: ... pKI1.1R plasmid (Addgene #85808) was linearized by incubating 1.5 µg of the plasmid with AarI restriction enzyme for 16 h ...
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bioRxiv - Synthetic Biology 2024Quote: ... RfxCas13d were amplified from pY108 (Addgene #84739), pXR001 (#84739) ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli DH10B M.XmnI (Addgene, 114269)34 and plasmid DNA was purified using the QIAprep Spin Miniprep Kit (Qiagen ...
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bioRxiv - Synthetic Biology 2024Quote: ... This plasmid is available from Addgene (#138589). A version of this plasmid encoding the Sav S112F K121Q mutant was used as the starting point for library generation.
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bioRxiv - Neuroscience 2024Quote: ... and pAAV-hSyn1-GcaMP6s-P2A-nls-dTomato (which was a gift from Jonathan Ting [Addgene plasmid # 51084; http://n2t.net/addgene:51084; RRID: Addgene_51084]), respectively8,34 ...
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bioRxiv - Neuroscience 2024Quote: ... gene fragments of GcaMP6s and P2A were obtained from pGP-CMV-GcaMP6s-WPRE (which was a gift from Douglas Kim & GENIE Project [Addgene plasmid # 40753 ...
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bioRxiv - Neuroscience 2024Quote: ... gene fragments of GcaMP6s and P2A were obtained from pGP-CMV-GcaMP6s-WPRE (which was a gift from Douglas Kim & GENIE Project [Addgene plasmid # 40753; http://n2t.net/addgene:40753; RRID: Addgene_40753]), and pAAV-hSyn1-GcaMP6s-P2A-nls-dTomato (which was a gift from Jonathan Ting [Addgene plasmid # 51084 ...
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bioRxiv - Synthetic Biology 2024Quote: ... carrying a previously constructed helper plasmid (24) (pDY118A, Figure S1, Addgene ID 182958). pDY118A encodes SsrA-tagged SpCas9 (10 ...
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bioRxiv - Synthetic Biology 2024Quote: ... sgRNAs containing 20-nucleotide (nt) spacer sequences to direct Cas9 cleavage were encoded on donor or guide plasmids constructed from pAM041 (Figure S2, Addgene ID 217969) as described below ...
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bioRxiv - Neuroscience 2024Quote: ... were cloned by ligating annealed oligonucleotide pairs (Supplemental table 5) into the BsmBI-digested pBPK1520 plasmid (BPK1520 was a gift from Keith Joung. Addgene#65777 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... with 0.5 µg of either a 1:1 mixture of the S plasmids and a Jun-Nt Venus fragment (Addgene 22012) plasmid or a mixture of hACE2 plasmid (kindly provided by Dr ...